LOCUS       NM_008971               2995 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus twinfilin actin binding protein 1 (Twf1), transcript
            variant 1, mRNA.
ACCESSION   NM_008971
VERSION     NM_008971.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Nguyen,M.T., Won,Y.H., Kwon,T.W. and Lee,W.
  TITLE     Twinfilin-1 is an essential regulator of myogenic differentiation
            through the modulation of YAP in C2C12 myoblasts
  JOURNAL   Biochem Biophys Res Commun 599, 17-23 (2022)
   PUBMED   35168059
  REMARK    GeneRIF: Twinfilin-1 is an essential regulator of myogenic
            differentiation through the modulation of YAP in C2C12 myoblasts.
REFERENCE   3  (bases 1 to 2995)
  AUTHORS   Takeda,S., Koike,R., Fujiwara,I., Narita,A., Miyata,M., Ota,M. and
            Maeda,Y.
  TITLE     Structural Insights into the Regulation of Actin Capping Protein by
            Twinfilin C-terminal Tail
  JOURNAL   J Mol Biol 433 (9), 166891 (2021)
   PUBMED   33639213
  REMARK    GeneRIF: Structural Insights into the Regulation of Actin Capping
            Protein by Twinfilin C-terminal Tail.
REFERENCE   4  (bases 1 to 2995)
  AUTHORS   Hakala,M., Wioland,H., Tolonen,M., Kotila,T., Jegou,A.,
            Romet-Lemonne,G. and Lappalainen,P.
  TITLE     Twinfilin uncaps filament barbed ends to promote turnover of
            lamellipodial actin networks
  JOURNAL   Nat Cell Biol 23 (2), 147-159 (2021)
   PUBMED   33558729
  REMARK    GeneRIF: Twinfilin uncaps filament barbed ends to promote turnover
            of lamellipodial actin networks.
            Erratum:[Nat Cell Biol. 2021 Apr;23(4):437-438. doi:
            10.1038/s41556-021-00651-8.. PMID: 33654262]
REFERENCE   5  (bases 1 to 2995)
  AUTHORS   Shekhar,S., Hoeprich,G.J., Gelles,J. and Goode,B.L.
  TITLE     Twinfilin bypasses assembly conditions and actin filament aging to
            drive barbed end depolymerization
  JOURNAL   J Cell Biol 220 (1) (2021)
   PUBMED   33226418
  REMARK    GeneRIF: Twinfilin bypasses assembly conditions and actin filament
            aging to drive barbed end depolymerization.
REFERENCE   6  (bases 1 to 2995)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 2995)
  AUTHORS   Paavilainen,V.O., Merckel,M.C., Falck,S., Ojala,P.J., Pohl,E.,
            Wilmanns,M. and Lappalainen,P.
  TITLE     Structural conservation between the actin monomer-binding sites of
            twinfilin and actin-depolymerizing factor (ADF)/cofilin
  JOURNAL   J Biol Chem 277 (45), 43089-43095 (2002)
   PUBMED   12207032
  REMARK    GeneRIF: structural conservation between its actin monomer-binding
            sites and the binding site of actin-depolymerizing factor
            (ADF)/cofilin
REFERENCE   8  (bases 1 to 2995)
  AUTHORS   Palmgren,S., Ojala,P.J., Wear,M.A., Cooper,J.A. and Lappalainen,P.
  TITLE     Interactions with PIP2, ADP-actin monomers, and capping protein
            regulate the activity and localization of yeast twinfilin
  JOURNAL   J Cell Biol 155 (2), 251-260 (2001)
   PUBMED   11604420
REFERENCE   9  (bases 1 to 2995)
  AUTHORS   Vartiainen,M., Ojala,P.J., Auvinen,P., Peranen,J. and
            Lappalainen,P.
  TITLE     Mouse A6/twinfilin is an actin monomer-binding protein that
            localizes to the regions of rapid actin dynamics
  JOURNAL   Mol Cell Biol 20 (5), 1772-1783 (2000)
   PUBMED   10669753
REFERENCE   10 (bases 1 to 2995)
  AUTHORS   Beeler,J.F., Patel,B.K., Chedid,M. and LaRochelle,W.J.
  TITLE     Cloning and characterization of the mouse homolog of the human A6
            gene
  JOURNAL   Gene 193 (1), 31-37 (1997)
   PUBMED   9249064
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147160.2.
            
            On Mar 23, 2022 this sequence version replaced NM_008971.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094034.1, AK150852.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                AC147160.2         88936-89030         c
            96-173              AC147160.2         87412-87489         c
            174-352             AC147160.2         85542-85720         c
            353-448             AC147160.2         84632-84727         c
            449-553             AC147160.2         83565-83669         c
            554-679             AC147160.2         81934-82059         c
            680-830             AC147160.2         80401-80551         c
            831-952             AC147160.2         80128-80249         c
            953-2995            AC147160.2         77132-79174         c
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 48.59 cM"
     gene            1..2995
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /note="twinfilin actin binding protein 1"
                     /db_xref="GeneID:19230"
                     /db_xref="MGI:MGI:1100520"
     exon            1..95
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..1123
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /note="isoform 1 is encoded by transcript variant 1; PTK9
                     protein tyrosine kinase 9; twinfilin-1; protein A6; actin
                     monomer-binding protein; twinfilin, actin-binding protein,
                     homolog 1"
                     /codon_start=1
                     /product="twinfilin-1 isoform 1"
                     /protein_id="NP_032997.3"
                     /db_xref="CCDS:CCDS27773.1"
                     /db_xref="GeneID:19230"
                     /db_xref="MGI:MGI:1100520"
                     /translation="MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVVGSCSP
                     PSDSWEQDYDSFVLPLLEDKQPCYVLFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYA
                     ATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKIN
                     EVQTDVSVDTKHQTLQGVAFPISRDAFQALEKLSKKQLNYVQLEIDIKNETIILANTE
                     NTELRDLPKRIPKDSARYHFFLYKHSHEGDYLESVVFIYSMPGYTCSIRERMLYSSCK
                     SPLLEIVERQLQMDVIRKIEIDNGDELTADFLYDEVHPKQHAHKQSFAKPKGPAGKRG
                     IRRLIRGPAEAEATTD"
     exon            96..173
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            174..352
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            353..448
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            449..553
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            554..679
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            680..830
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            831..952
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     exon            953..2995
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2976..2981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /note="hexamer: AATAAA"
     polyA_site      2995
                     /gene="Twf1"
                     /gene_synonym="A6; Ptk9; twinfilin"
                     /note="major polyA site"
ORIGIN      
        1 acttcctgga tccgccggcc ggggccgcta ccgcaccctg cgccggagcc gggagcctgg
       61 ggccgccgcc atgtcccacc agacaggcat ccaagcaagc gaagatgtca aagagatctt
      121 tgcccgagcc agaaacggga aatacagact tctgaaaata tctatcgaaa atgagcaact
      181 ggtggttggc tcctgtagtc caccttcaga ctcctgggaa caggactacg actcctttgt
      241 cttgcccctg ctggaggaca agcagccgtg ctatgtatta ttcaggttag actctcagaa
      301 tgcccaggga tatgaatgga tattcattgc atggtctcca gatcattctc atgtacgtca
      361 gaaaatgctg tatgcggcaa caagagcgac tctgaagaaa gaatttggag gtggccacat
      421 taaagatgaa gtgtttggaa cagtaaagga ggacgtatca ttacatggat ataaaaagta
      481 tttgctgtcc cagtcttccc ctgccccgct gaccgccgct gaggaagaat tacggcagat
      541 taaaatcaac gaggtccaaa ctgatgtgag tgtggacaca aagcaccaga cactccaagg
      601 cgtagccttt cctatttctc gagatgcctt ccaggctttg gagaagctga gtaagaagca
      661 gctcaactat gtgcagttgg aaatagatat aaaaaatgaa acaataattt tggccaacac
      721 agaaaataca gaactgagag atttgccaaa gaggattccc aaggattcag cgcgttacca
      781 tttctttctg tacaagcatt cccacgaagg agactactta gagtccgtag tttttattta
      841 ttcgatgcct ggatacacgt gcagcataag agagcggatg ctgtattcca gctgcaagag
      901 ccctctgctc gagattgtag agcgacagct acagatggat gtgatcagaa agattgagat
      961 agacaatggg gatgagctga ctgcggattt cctgtacgat gaagtccacc ccaagcagca
     1021 tgcacataag cagagttttg ctaaaccgaa aggtcctgcg ggaaagaggg ggatccgaag
     1081 attgattagg ggcccagcgg aagcagaagc cactactgac tagaactgtc gtattaaatg
     1141 ttgcaatata gttttgtaaa gtttccagct tttagtacaa ggagaactga aatcattcca
     1201 tgttgataga gttgggagga aaattgtact ttttgaaaaa tagcactttt cacttctatg
     1261 tattttttaa attaatgcta tagaagactc atgattttta ttttttagtt aaaactagac
     1321 aaggtttcaa catagtgatg tttggttttg tcacactgtt ttcataacgt gttgattcca
     1381 cactttcaca taatcttcaa tatttcccat aggtgggcca gttccaccaa gcataaggtc
     1441 ttgctctgta gaactctgta gtgaggtcta acgttcccta tgaagggaag cgaacgacga
     1501 acaaccctga gatactcaca cctgtcgagt gtgttaagga gcctgggagg aggcgggcag
     1561 ctgattgctt tgagcatgct caggaagggc tgtctgccgc ggtggagaaa ggcaaactgc
     1621 agcagggctt cctgtccctc ctctccattg tagtagcatc agatctcaga aacgaagttc
     1681 acactgaagc tcgaaaggta ccagacagtg caggcttccc atcccatccc ccgcccccct
     1741 gcactggaag actcgcgtcc agtaaggtct ttgcagaact ttcgagctat ctatttccaa
     1801 agtgagtcca gagggctgac gccagacgtt catgttcgaa agccaaggat cgctcaaaaa
     1861 aaaacaaaac taaagaaaaa tgttggaagt ttcaaggggt tttttttttt ttttttttgg
     1921 ttttgtgttt tgtttttgtt tttgtttttc tctttgaaac catgtatctt aaagcaaatt
     1981 gtcctgttgc ttgggagcaa gtgttcagat ctaaaattat tgatttctac tacttagtag
     2041 gctgacacag ataatactta gcccagggat cttgtttcag tgaataatgt ctcagaacac
     2101 gggttgtctg aagctttgtg ttttcagtta caacctagag agtttagagc ctcaagagtt
     2161 ctttgttact tgaaatagag ggagttagaa cacttggtga tttcatctgc taaacctgct
     2221 gcagagccat aactgagaag ttctgaagtg gaccatcgtg tccaggacct ggaagtcctc
     2281 gacctgaact tcatgctgca caagataatg tcagtaatgt acatttcatg gtatgaaata
     2341 tgaagcaccc attgcagagc cttattatct aatacacacg cctttcagaa attcagcgtg
     2401 tattcacata gaccataaga ggaattacag gctgatggaa attttttaaa atagatttga
     2461 ttattgtgtg ttttgtttgt ttacaaatac gtagacttta ctacttacat atgcaggaca
     2521 gtcgacagtg acgtcacact tgtttacgag aggtaaactt tttagttgta cgtgtgtaca
     2581 gttgtcacac gtgccataga gtgctgcact gagagtcctg gagccctgtc tagaccatca
     2641 ccacagctgc cacttcaagc agagggagca ctacgtgtcc tggatccaga gccagtgggg
     2701 gactgttctg aggcatcacg tgcaacgatg ctctgaagcc tagtacacta catcgaagtc
     2761 aatgcacatt ccagaatata gatgtgatcc tgtaacccta ctaacgatta aaccaatgat
     2821 tgctgagaat cacgtatgca tttgttttag ggtataactc attgtttaca ctatgtttta
     2881 ggtgacaata gatgtgaata acatccccaa taaatttata tagcagtgaa aaattacatg
     2941 tcttctgtgg acattttata agtgcatttt atatcaataa aatttttctc tttga
//
