LOCUS       NM_175472               6070 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus terminal uridylyl transferase 4 (Tut4), transcript
            variant 3, mRNA.
ACCESSION   NM_175472 XM_620089 XM_886995 XM_897175 XM_897183 XM_897194
            XM_897202 XM_897210 XM_897221 XM_897225 XM_897236 XM_916010
            XM_924060 XM_924064 XM_924066 XM_924069 XM_924071 XM_924077
            XM_924079 XM_924083 XM_924089
VERSION     NM_175472.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Piasecka,A., Szczesniak,M.W., Sekrecki,M., Kajdasz,A.,
            Sznajder,L.J., Baud,A. and Sobczak,K.
  TITLE     MBNL splicing factors regulate the microtranscriptome of skeletal
            muscles
  JOURNAL   Nucleic Acids Res 52 (19), 12055-12073 (2024)
   PUBMED   39258536
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6070)
  AUTHORS   Gupta,A., Li,Y., Chen,S.H., Papas,B.N., Martin,N.P. and Morgan,M.
  TITLE     TUT4/7-mediated uridylation of a coronavirus subgenomic RNAs delays
            viral replication
  JOURNAL   Commun Biol 6 (1), 438 (2023)
   PUBMED   37085578
  REMARK    GeneRIF: TUT4/7-mediated uridylation of a coronavirus subgenomic
            RNAs delays viral replication.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   Sha,Q.Q., Zhu,Y.Z., Li,S., Jiang,Y., Chen,L., Sun,X.H., Shen,L.,
            Ou,X.H. and Fan,H.Y.
  TITLE     Characterization of zygotic genome activation-dependent maternal
            mRNA clearance in mouse
  JOURNAL   Nucleic Acids Res 48 (2), 879-894 (2020)
   PUBMED   31777931
REFERENCE   5  (bases 1 to 6070)
  AUTHORS   Morgan,M., Kabayama,Y., Much,C., Ivanova,I., Di Giacomo,M.,
            Auchynnikava,T., Monahan,J.M., Vitsios,D.M., Vasiliauskaite,L.,
            Comazzetto,S., Rappsilber,J., Allshire,R.C., Porse,B.T.,
            Enright,A.J. and O'Carroll,D.
  TITLE     A programmed wave of uridylation-primed mRNA degradation is
            essential for meiotic progression and mammalian spermatogenesis
  JOURNAL   Cell Res 29 (3), 221-232 (2019)
   PUBMED   30617251
REFERENCE   6  (bases 1 to 6070)
  AUTHORS   Jones,M.R., Blahna,M.T., Kozlowski,E., Matsuura,K.Y., Ferrari,J.D.,
            Morris,S.A., Powers,J.T., Daley,G.Q., Quinton,L.J. and Mizgerd,J.P.
  TITLE     Zcchc11 uridylates mature miRNAs to enhance neonatal IGF-1
            expression, growth, and survival
  JOURNAL   PLoS Genet 8 (11), e1003105 (2012)
   PUBMED   23209448
  REMARK    GeneRIF: We conclude that the Zcchc11-mediated terminal uridylation
            of mature miRNAs is pervasive and physiologically significant,
            especially important in the neonatal period for fostering IGF-1
            expression and enhancing postnatal growth and survival
REFERENCE   7  (bases 1 to 6070)
  AUTHORS   Hagan,J.P., Piskounova,E. and Gregory,R.I.
  TITLE     Lin28 recruits the TUTase Zcchc11 to inhibit let-7 maturation in
            mouse embryonic stem cells
  JOURNAL   Nat Struct Mol Biol 16 (10), 1021-1025 (2009)
   PUBMED   19713958
  REMARK    GeneRIF: Lin28 recruits the TUTase Zcchc11 to inhibit let-7
            maturation in mouse embryonic stem cells
REFERENCE   8  (bases 1 to 6070)
  AUTHORS   Jones,M.R., Quinton,L.J., Blahna,M.T., Neilson,J.R., Fu,S.,
            Ivanov,A.R., Wolf,D.A. and Mizgerd,J.P.
  TITLE     Zcchc11-dependent uridylation of microRNA directs cytokine
            expression
  JOURNAL   Nat Cell Biol 11 (9), 1157-1163 (2009)
   PUBMED   19701194
  REMARK    GeneRIF: Zcchc11 fine tunes IL-6 production by uridylating miR-26a.
REFERENCE   9  (bases 1 to 6070)
  AUTHORS   Minoda,Y., Saeki,K., Aki,D., Takaki,H., Sanada,T., Koga,K.,
            Kobayashi,T., Takaesu,G. and Yoshimura,A.
  TITLE     A novel Zinc finger protein, ZCCHC11, interacts with TIFA and
            modulates TLR signaling
  JOURNAL   Biochem Biophys Res Commun 344 (3), 1023-1030 (2006)
   PUBMED   16643855
  REMARK    GeneRIF: We propose that ZCCHC11 is a unique TLR signal regulator,
            which interacts with TIFA after LPS treatment and suppresses the
            TRAF6-dependent activation of NF-kappaB.
            Erratum:[Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121]
REFERENCE   10 (bases 1 to 6070)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627238.15 and AL626783.16.
            
            On Apr 10, 2020 this sequence version replaced NM_175472.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC150791.1, SRR1660813.133460.1
                                    [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN01164131, SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-230               AL627238.15        186004-186233
            231-414             AL627238.15        186611-186794
            415-1283            AL627238.15        205467-206335
            1284-1447           AL626783.16        4668-4831
            1448-1564           AL626783.16        9562-9678
            1565-1742           AL626783.16        13364-13541
            1743-1831           AL626783.16        15181-15269
            1832-1883           AL626783.16        17561-17612
            1884-1953           AL626783.16        21180-21249
            1954-2080           AL626783.16        24038-24164
            2081-2200           AL626783.16        26121-26240
            2201-2413           AL626783.16        26435-26647
            2414-2588           AL626783.16        30250-30424
            2589-3283           AL626783.16        31086-31780
            3284-3434           AL626783.16        35597-35747
            3435-3521           AL626783.16        38421-38507
            3522-3625           AL626783.16        41418-41521
            3626-3683           AL626783.16        44982-45039
            3684-3787           AL626783.16        45130-45233
            3788-3877           AL626783.16        47431-47520
            3878-3991           AL626783.16        49050-49163
            3992-4069           AL626783.16        60225-60302
            4070-4247           AL626783.16        60480-60657
            4248-4295           AL626783.16        60876-60923
            4296-4378           AL626783.16        61008-61090
            4379-4494           AL626783.16        66269-66384
            4495-4667           AL626783.16        67455-67627
            4668-4768           AL626783.16        68927-69027
            4769-5216           AL626783.16        73596-74043
            5217-5369           AL626783.16        75536-75688
            5370-6070           AL626783.16        76929-77629
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 50.55 cM"
     gene            1..6070
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /note="terminal uridylyl transferase 4"
                     /db_xref="GeneID:230594"
                     /db_xref="MGI:MGI:2445126"
     exon            1..230
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            231..414
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            415..1283
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     CDS             506..5440
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /EC_number="2.7.7.52"
                     /note="isoform 3 is encoded by transcript variant 3;
                     terminal uridylyltransferase 4; TUTase 4; zinc finger,
                     CCHC domain containing 11; zinc finger CCHC
                     domain-containing protein 11"
                     /codon_start=1
                     /product="terminal uridylyltransferase 4 isoform 3"
                     /protein_id="NP_780681.2"
                     /db_xref="CCDS:CCDS18451.1"
                     /db_xref="GeneID:230594"
                     /db_xref="MGI:MGI:2445126"
                     /translation="MEEPKTSKNENHEPKKNIICEESKAVKIISNQTLKPRNDKSEIG
                     TSSLNRNSSKKTKQNDICIEKTEAKSCKVNAASVPGPKDLGLVHRDQSHCKMKKLPNS
                     PMKAQKGSSQTKLEKTPSLQTKAEKVPKSPNLPVKAEKAPCTTAEATTEKALNSQRKE
                     ENTPTSQMKLQKTPRSPLEPENVPSLLLKENVKQTESQQTGKKLTSSFVSMDKRKSEA
                     LQGEKSALENSSLSQKQQTQTDNIADSDDSASGIEDTADDLSKMKSEESNKENSSEMD
                     YLENATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIE
                     NIQGAHKHIKEKRHKKNILEKQEESELRSLPSPSSAHLAALSVAVVELAKEQGITDDD
                     LRIRQDIVEEMSKVIMTFLPECSLRLYGSSLTKFALKSSDVNIDIKFPPKMNHPDLLI
                     QVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGK
                     VEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWI
                     EGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETD
                     NQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRE
                     NKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGGNKSTM
                     DPKKKEKGKLSSKKPVKSDCSATNCCILGESAEKIHMERGQPAKHDETEFTSQRCIVD
                     NDSLLVNELGLANHGQDSSSLSTASGGSDLKQKSAEKQGDLTPSETSLKKELSQCICI
                     GTPDGAESAGTDCRSNLEMDSSHQIVCNNVSATSCNCKATEVTSDLVDEDNLPSQELY
                     YVFDKFILTSGKPPTIVCSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVC
                     KRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDIC
                     MTLEGHENAEKLNCKEIIENLAKILKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDI
                     SLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVL
                     YFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGE
                     LWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSR
                     KMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAPNDRCCRVCGKIGH
                     YMKDCPKRKRLKKKDSEEEKEGNEEEKDSRDLLDSRDLRCFICGDAGHVRRECPEVKM
                     ARQRNSSVAAAQLVRNLVNAQQVAGSAQQQSDQSIRTRQSSECSDSPSYSPQPQPFPQ
                     NSPQPSALPPPPSQPGSQPKLGPPQQGGQPPHQVQMPLYNFPQSPPAHYSPMHSMGLL
                     PMHPLQIPAPSWPIHGPMLHSAPGSTPSNIGLNDPSIIFAQPAARPMAIPSPSHDGHW
                     PRTVAPNSLVNNGAVGNSEPRFRGLNPPIPWEHAPRHFPLVPASWPYGLHQNFMHQGN
                     PRFQPKPFYAQADRCATRRCRERCPHPPRGNVSE"
     exon            1284..1447
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1564
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1742
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1831
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1883
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..1953
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2080
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2200
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2413
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2588
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..3283
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3284..3434
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3521
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3522..3625
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3626..3683
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3684..3787
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3788..3877
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..3991
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            3992..4069
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4070..4247
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4248..4295
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4296..4378
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4379..4494
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4495..4667
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4668..4768
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            4769..5216
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            5217..5369
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     exon            5370..6070
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6051..6056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /note="hexamer: AATAAA"
     polyA_site      6070
                     /gene="Tut4"
                     /gene_synonym="6030404K05Rik; 9230115F04Rik; mKIAA0191;
                     PPAPD3; Tent3a; Zcchc11"
                     /note="major polyA site"
ORIGIN      
        1 aagctcgccg gcgtcctcgc gccgcttccc ggcgtcggcg tgctggaccg caggaccctc
       61 tctctctctt tctctctccc tgcaggcccg ctccagccag cgagccgcgg gtagctccga
      121 ccctttccct ccctccctcc cttcctctct ccctcggggg cccgcccgcc cccagctcgg
      181 gcgccgatcg gagcccgcgc gcgctcgccc gctctccctc ctcagcgcgg ttcgcggcgc
      241 ggcggacatg gcggtggagt cctgagctcg tcgtgtcctg gcctccatcg gcggcggcga
      301 cggcggcggc ggcgacggcg acgggggcgg cagcggcggc gggaggcgga gggggagccg
      361 gagcctctga gatgaggagg cgtcgcgcgc gcctgtagct cgcgggcttc tcagtttgct
      421 tgcctaaagt agatttggag cagttcatac acctgagccc tgctattcta cagcgtcaga
      481 ttggaaagag tcagattttc aaataatgga agagcccaag acttctaaaa acgaaaatca
      541 tgaaccaaag aagaatatta tttgtgaaga aagcaaagca gttaaaatta tatctaatca
      601 aaccttgaag cctagaaatg ataagtcaga aattgggacc agctctctga atagaaatag
      661 tagtaaaaaa accaagcaaa atgatatttg tatagaaaaa acagaagcta aatcatgtaa
      721 agtaaacgct gccagtgttc caggccctaa agatttgggg ttagtacatc gagatcaaag
      781 tcattgcaaa atgaaaaagt taccaaattc acccatgaaa gcccagaagg gatcttcaca
      841 gacaaaatta gaaaagacac ctagtttaca gacaaaagca gaaaaagtac caaagtctcc
      901 aaatttacca gtaaaagcag aaaaagcgcc ctgtacaaca gcagaagcaa caacagaaaa
      961 agcacttaat tcacagagaa aagaagaaaa cacacccact tctcagatga aattacagaa
     1021 gacacccaga tcacctttag agccagaaaa tgtgcccagt ttactattga aagaaaatgt
     1081 gaaacagaca gaatcacaac agactgggaa gaaacttaca agctcctttg tttctatgga
     1141 taaaaggaag agtgaagctc tacagggaga aaagtcagct ctggaaaact catcactatc
     1201 tcagaagcag cagactcaga cagataatat tgctgattct gatgacagtg cttcagggat
     1261 tgaagataca gcagatgatt tgagtaaaat gaagagtgag gaatctaaca aagaaaattc
     1321 ttctgagatg gactatttag aaaatgccac tgtaatagat gaatctgctt tgacacctga
     1381 gcagaggcta ggcttgaaac aagcagaaga acgtctagaa agagatcaca tcttccgact
     1441 tgaaaaacgg tcaccagaat ataccaattg ccgctatcta tgcaaacttt gcttaattca
     1501 cattgaaaac atccaaggag cacataaaca tataaaagaa aaacgacata agaaaaatat
     1561 tttggaaaaa caagaagaga gtgaacttcg gtctcttcca tctccttcct ctgctcactt
     1621 ggctgcttta agtgttgcag ttgttgagtt agcaaaagaa caaggaataa cagatgatga
     1681 cctcagaatc cgccaggaca ttgtggagga aatgtcaaag gttataatga catttttacc
     1741 agaatgttca ctgaggttgt atggttcatc tctgacaaag tttgctctga aaagcagtga
     1801 tgttaatata gatataaaat ttcctcccaa gatgaatcat ccagatcttc tgatacaagt
     1861 gcttggaatt ctgaaaaaaa gtgcattata tatagatgtg gaatctgatt ttcatgctaa
     1921 agttcctgtt gtggtgtgca aagatcggaa aagtgcttta ctgtgtagag taagtgcagg
     1981 aaatgatatg gcgtgtctca caactgactt acttgctgct cttggcaaag tggaacccgt
     2041 cttcactccc ttagtgctag cttttcgcta ttgggctaag ctgtgctata ttgactccca
     2101 aactgatggt ggaatccctt cttactgctt tgctttgatg gtgatgtttt ttcttcaaca
     2161 aagaaaaccc cctcttcttc cttgcttact tggaagttgg attgaaggct ttgacccgaa
     2221 aagaatggat gactttcagc tgaagggcat agtagaagag aagtttgtga agtgggaata
     2281 taattcaagt agtgcaactg agaaaaactt aattgctgat gaaaacaaag ctaaggcaga
     2341 cgaaccaaaa gatgatacca agaagacaga aacagacaac caaagtaatg ctgcgaaggc
     2401 aaaacatggc aaatctcctt taacattgga agcaccaaat caggtaccct tgggacagtt
     2461 gtggttggag ctgctaaaat tttacacact ggattttgct ttggaggaat atgtcatatg
     2521 tgtacggata caagatattc taactagaga gaacaaaaac tggcctaaaa ggcggatagc
     2581 cattgaagat ccattttcag tcaagaggaa tgttgcccgg agcttaaaca gccagcttgt
     2641 atacgaatat gttgtggaaa gattcagagc agcttatcga tattttgcct gtcctcaaaa
     2701 gaagggtgga aataaatcta caatggatcc taagaagaaa gagaagggga agctaagcag
     2761 taagaaacca gtgaagtctg attgttcggc aaccaattgt tgcattcttg gggaaagtgc
     2821 agaaaaaata catatggaaa gaggccaacc tgctaaacat gatgaaacgg agtttacatc
     2881 acagagatgt attgttgata atgacagttt attggtaaat gaactaggtt tggctaacca
     2941 tggacaagat tcttcatctc tttctactgc cagtggaggc agtgacttaa agcaaaaatc
     3001 agctgagaaa caaggtgatt taacaccttc agaaacttcc ttaaagaagg aactcagcca
     3061 gtgtatttgc attgggaccc ctgatggagc tgaatctgct ggaacagact gcagatcaaa
     3121 cttagaaatg gacagttcac atcagattgt gtgcaacaat gtatctgcta cctcttgcaa
     3181 ctgcaaagct acagaagtta cttctgacct tgttgatgag gataacctcc cctcccagga
     3241 attatattat gtgtttgata agttcatttt aacctctggc aagccgccaa caatagtatg
     3301 cagcatctgc aaaaaggatg gccattcaaa aaatgattgt ccagaggatt tcaggaaaat
     3361 tgatctaaaa cctctaccac caatgacaaa ccgatttcga gaaatacttg atttagtatg
     3421 taaaagatgt tttgatgaat tatcaccacc ttgctctgaa caacacaaca gggaacaaat
     3481 tttaattggc ttggaaaagt ttattcaaaa agaatatgat gaaaaagcaa gattgtgctt
     3541 atttggttct tctaagaatg gatttggatt ccgagatagt gatctggata tttgtatgac
     3601 tctggaaggc catgaaaatg cagagaaatt aaactgtaaa gaaataattg aaaatttggc
     3661 gaaaattctt aagaggcatc caggtttaag aaacattctg cctataacta ctgccaaagt
     3721 gcctattgta aaatttgaac acaggagaag tggactagaa ggtgatatca gtttatataa
     3781 cacattggct caacacaaca caagaatgct agctacttat gcagctattg atcctagagt
     3841 acagtacttg ggatatacta tgaaagtgtt tgcaaagcgc tgtgacattg gagatgcatc
     3901 tcggggaagt ttatcttcat atgcttacat ccttatggtg ctgtactttc tgcagcaaag
     3961 aaagccacca gttatcccag ttctacaaga gatctttgat gggaagcaga ttccgcagag
     4021 aatggttgat ggatggaatg cctttttctt tgataaaaca gaagagctga aaaagcgttt
     4081 accttcactt gggaagaaca cggaatcact gggagagctt tggttaggac tgcttcgctt
     4141 ctatacagaa gagtttgatt ttaaggagta tgtaatcagt attcggcaga agaagctgct
     4201 gaccactttt gaaaagcagt ggacttctaa atgcattgca attgaagatc cttttgactt
     4261 gaatcataac cttggtgctg gtgtttctag aaaaatgact aattttatta tgaaggcgtt
     4321 tatcaatgga aggaaacttt ttggtacccc cttttatcca ctcattggca gagaagctga
     4381 gtacttcttt gattcaagag tactaacaga tggagagttg gctcctaatg atcgttgttg
     4441 ccgtgtgtgt ggaaaaatag gtcactacat gaaagactgt cccaaaagga aaagactaaa
     4501 gaagaaagac agtgaagaag agaaggaagg gaacgaagaa gaaaaagact cccgtgacct
     4561 tcttgactcc cgagacctta ggtgtttcat atgtggagat gctggacatg ttcgccgcga
     4621 atgcccagag gtcaagatgg cccgccagag gaatagtagt gtggcagcag cccagctggt
     4681 ccgtaacctt gtaaatgctc agcaggtggc tggttcagcg cagcaacaga gtgaccagtc
     4741 cataaggacc agacagtctt cagaatgttc tgattcacca tcttattctc ctcagcctca
     4801 gccttttcca cagaattctc cccaaccatc tgctcttccc ccacctccat cccagccagg
     4861 atcccagcct aagcttggcc caccccagca gggagggcag ccccctcatc aggttcagat
     4921 gcccttgtat aactttcctc agtcacctcc agctcattat tcacccatgc acagtatggg
     4981 gttactgcca atgcaccccc tgcagatccc tgccccatcg tggcccatcc acggcccgat
     5041 gctgcactct gcacctggca gcacccccag caacattggc ttgaatgatc ccagcatcat
     5101 ctttgcacag cctgctgcca ggcccatggc aattcccagc ccttctcacg atggacactg
     5161 gcctcggacc gtggctccca attccctggt gaacaatggt gcagtgggta attcggagcc
     5221 acgctttcga ggactaaatc ctccaattcc atgggaacat gcaccacgcc atttccccct
     5281 tgtcccagct tcgtggcctt acggtttgca tcaaaatttc atgcatcagg gaaatccaag
     5341 atttcagccc aaacccttct atgctcaagc agacagatgt gccacgcgtc gctgtagaga
     5401 gcgctgtccc cacccaccac gaggaaacgt gtccgagtaa tgccagccca cagcaaccag
     5461 ccaatcctgc tgtctcaagg gtatccgtac ctcagtgtca gttacactca gcagaaaaag
     5521 tgacattaag gaaaacaaaa caaatcaggt tctgatttaa aattttaaca cacgtttaga
     5581 tagcttattt aaattctaag actgttttta aacactgtat tatttgtata taaatatgaa
     5641 ctatagtttt tactggtatc actaaattga gtgatacaaa tttcatctat tttattaccc
     5701 tatttttaag ggaattttat gttttgttta accttgatga attgtataaa gagagaattt
     5761 aaaaaattac attctttatt ttctattagc tgtattcata ctttggttgc ttcagacttt
     5821 atatatattg ttcttaagga ttaggaaagg atttcatgtg ttttaaattt gtcacttcta
     5881 ttctttacag aaccttgtag tgccaaaaac tttttaaaag gtcaaaaaaa aaaataaaat
     5941 tacaacataa agattctgaa atgtttttcc tttacatact tgtatattga atatattcaa
     6001 atttgaatgg aaagttgctt ttgtctgtat ttgaagttct attttaagtt aataaatctt
     6061 tttgacaaga
//
