LOCUS       NM_030254               3486 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus tumor suppressor candidate 3 (Tusc3), transcript
            variant 1, mRNA.
ACCESSION   NM_030254
VERSION     NM_030254.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3486)
  AUTHORS   Horiuchi,K., Perez-Cerezales,S., Papasaikas,P., Ramos-Ibeas,P.,
            Lopez-Cardona,A.P., Laguna-Barraza,R., Fonseca Balvis,N.,
            Pericuesta,E., Fernandez-Gonzalez,R., Planells,B., Viera,A.,
            Suja,J.A., Ross,P.J., Alen,F., Orio,L., Rodriguez de Fonseca,F.,
            Pintado,B., Valcarcel,J. and Gutierrez-Adan,A.
  TITLE     Impaired Spermatogenesis, Muscle, and Erythrocyte Function in U12
            Intron Splicing-Defective Zrsr1 Mutant Mice
  JOURNAL   Cell Rep 23 (1), 143-155 (2018)
   PUBMED   29617656
REFERENCE   4  (bases 1 to 3486)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   5  (bases 1 to 3486)
  AUTHORS   Kelleher,D.J., Karaoglu,D., Mandon,E.C. and Gilmore,R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132766.1, BC069898.1, AK081223.1 and BP765707.1.
            
            On Oct 25, 2017 this sequence version replaced NM_030254.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028182.1, SRR1660821.129557.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AK132766.1         2-23
            23-1535             BC069898.1         1-1513
            1536-3345           AK081223.1         425-2234
            3346-3486           BP765707.1         1-141               c
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 23.89 cM"
     gene            1..3486
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="tumor suppressor candidate 3"
                     /db_xref="GeneID:80286"
                     /db_xref="MGI:MGI:1933134"
     exon            1..356
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..1262
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; putative prostate cancer tumor suppressor;
                     magnesium uptake/transporter TUSC3; oligosaccharyl
                     transferase subunit TUSC3;
                     dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3 isoform 1 precursor"
                     /protein_id="NP_084530.3"
                     /db_xref="CCDS:CCDS85529.1"
                     /db_xref="GeneID:80286"
                     /db_xref="MGI:MGI:1933134"
                     /translation="MSARAAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKK
                     KENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFVKAPPRNYSMIVMFTALQPQRQCSVC
                     RQANEEYQILANSWRYSSAFCNKLFFGMVDYDEGTDVFQQLNMNSAPTFMHFPSKGRP
                     KRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLR
                     RNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQ
                     FVAESHIILVLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFR
                     SKYHGYPYSFLIK"
     exon            357..526
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            527..644
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            645..785
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            786..926
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1016
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1080
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1155
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1246
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..3471
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1512..1517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: AATAAA"
     polyA_site      1535
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="major polyA site"
     regulatory      1693..1698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: TATAAA"
     polyA_site      1713
                     /gene="Tusc3"
                     /gene_synonym="N33"
     regulatory      2039..2044
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: AATACA"
     polyA_site      2061
                     /gene="Tusc3"
                     /gene_synonym="N33"
     regulatory      2494..2499
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: AATAAA"
     polyA_site      2529
                     /gene="Tusc3"
                     /gene_synonym="N33"
     regulatory      2689..2694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: ATTAAA"
     polyA_site      2694
                     /gene="Tusc3"
                     /gene_synonym="N33"
     polyA_site      2719
                     /gene="Tusc3"
                     /gene_synonym="N33"
     regulatory      2734..2739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: ATTAAA"
     polyA_site      2755
                     /gene="Tusc3"
                     /gene_synonym="N33"
     regulatory      3441..3446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tusc3"
                     /gene_synonym="N33"
                     /note="hexamer: AATAAA"
     polyA_site      3467
                     /gene="Tusc3"
                     /gene_synonym="N33"
ORIGIN      
        1 atcccgcgcc attcttgtcc ctctcctggt gaaccggatg cgctgtcagt ctcctcctcc
       61 tccgcgtcct tagcctccac ccagctccct ggagaagccg ctgccacccc gaggccttgg
      121 ccaggagagg ctgctggcga ctggcctggc gggcgcgggc acggtaggag ctgggcgcgc
      181 acggctaccg cgcgtggagg agacacagcc ctgcggccat gagtgccagg gccgctcctt
      241 cacgccggag acaggcgggc cgccggttgc gctacctgcc tactggtagc tttcccttcc
      301 tcctcttgct gctgctgctc tgcatccagc tcgggggcgg acagaagaaa aaggagaacc
      361 ttttggctga aaaagtggag cagctgatgg aatggagttc caggcgctcg atcttcagaa
      421 tgaacggcga caagttccgg aagtttgtaa aagccccacc tcggaactac tccatgatcg
      481 tcatgttcac tgctctgcag cctcagcggc agtgttctgt gtgcaggcag gctaacgaag
      541 aatatcaaat cctggctaat tcctggcgtt attcatctgc tttttgcaac aaactgtttt
      601 ttggaatggt ggactatgat gaagggacag atgtttttca acagctcaac atgaactccg
      661 ctcccacatt catgcatttt ccttcaaaag gcagacccaa gagagctgat acttttgacc
      721 ttcaacgaat tggatttgca gctgagcagc tagcaaaatg gattgccgac aggacggatg
      781 ttcatattcg agttttccgg ccacccaact actcaggcac cattgctttg gccctgttag
      841 tgtcactggt tggtggcttg ctttatctaa ggaggaacaa cttggagttt atctataaca
      901 agactggttg ggccatggta tctctgtgta tagtctttgc tatgacgtct ggccagatgt
      961 ggaatcatat ccgtggacct ccatatgctc ataagaaccc acacaatgga caagtgagct
     1021 acattcatgg aagcagccag gctcagtttg tggcagagtc acacatcatt ctagtactga
     1081 atgctgctat caccatgggg atggttcttc taaatgaagc agcaacttcc aaaggggatg
     1141 tcgggaaaag acgcatcatt tgcctcgtgg gattgggctt ggtggtcttc ttcttcagtt
     1201 ttctcctttc aatatttcgt tcaaagtacc atggctatcc atatagcttt ttaattaaat
     1261 gaagccaaat gggatttgcg taaagtgagt gtttactgtg aagagaagca gttcctgaca
     1321 ttatattatt tggaattctt gaggttcatt ctttgttgat tgtgatatac tagcttattc
     1381 ttgtataatt tttttaaact gtggattttc ctaaaacatt taatttatag aaatggatgt
     1441 gtaacattcc ataatccgca atggactatc aaagtgtttt taaatattct caatgtatgc
     1501 cacaggagtg aaataaatga cattgtaact atgaattcat gttttagaat tgtgtgtgct
     1561 cccatgtaag gaaaccactt gttagtaaag aaatccatgg attatatgta aaagaattgc
     1621 tagtactgta caaggataga gcatatgcat gaaatatgtc gtgtggacaa taattcttac
     1681 ttcagtttca cttataaagg atgaacactg ttacaaaagt gttttgttat tcttcttcac
     1741 aactagatca taaatacata ggacaaagag gtggataagt gttaatgcca gttcagatcc
     1801 ttaacctaaa gaacgaagct agaaggaacc ttagacttca ccatacctcc aatggagtca
     1861 ggcaatagaa ccatgtgtca ttattcaatg tttttgtgaa gctctttaca aattctgact
     1921 catgaaatcc aatctactac ggtcactttc cttttgttta atggaccggg cagggacctg
     1981 gatccctatg aattgttagt tttcccagaa tgactcagat gaagcttctt gataaccaaa
     2041 tacagaagga atgtaactat tgttattgtt tgttgttaac gaacgtaact gtgtatagca
     2101 tacccatgta catcccagta ttcctcacat cttttcaata gacaggcaat gcaagaactt
     2161 ttctgtgagt tatagagata gtagaaacca gctgaaaacc aaaatataag ttgtacttat
     2221 aatcagcctt ttaaacattt tttgtaaaac tctgtactgc aactgaaaga aatatttcct
     2281 taattggttc agtaagtttt aacattttta aaagcttata tctgaatagt aaaactgtgg
     2341 agtgatttat ccagtggcaa tcccagctgt atagtcacaa ataggagtaa tactattctc
     2401 tgaatttgca taataattta gaactacatt aactttatca tactctgata tctcttacta
     2461 aagcatagtt tccttagttc acttaagaga tctaataaac tcagcattga gattcctttt
     2521 atggaaaaat tcggcataat tttctgttcg atgcctgtcc ctaggtatca tttgaattgg
     2581 agttgtacca ttcaactatt gctatagtct caaacttaac tgactctgaa cttcagagtg
     2641 caaatggaat ttgtttccag accttaaaaa taacagatat ttgttataat taaatgtcgt
     2701 cattccctat atctcaaaat gatcttctag ttaattaaat catattgaca aaacacgtca
     2761 aatctcactt taaaggctaa tagtatatat gcataacccc agccaggcca tccaagccca
     2821 tggaatggag gtttggaggt agatatttct ttatcttaca ttattaagaa attttccagt
     2881 ttgtaacaat ttgtaatttt cttaagatca aaaagaagtt aattaattac aggtttgttc
     2941 atggggtatg aggccagatc caaggggctc cagtgcctct gggccttcca ggcacctgca
     3001 cttgtgtgta catatgcaaa cacattcaca aaaaaagaaa aacagttcaa gttttctttt
     3061 tcataatttc atatcttaga atgggacagg tggggcaaaa gagatggctc accaggtaaa
     3121 tgttgcttac tgttcttcca gaagaccagg gtatggttcc cagccttcag caggtgcctt
     3181 cctaccagct accgataact gcagttccat ggctctgcca cttttaacct ttattcactg
     3241 tattgagaat tttcattcaa atcaatcata catttgacag tttctcacta gaatttaaaa
     3301 attacttttc tcctgtgaca gaaaagtcct gatagaaaaa aaaaatctcc tttcagggag
     3361 tctcatttta tagagacatt tgttggtcat ctccttgtgt tgtcttcagc atagtgcttc
     3421 ttgaattatg cattcaaaca aataaaccca atactatgtg ccaaccataa aaaaaaaaaa
     3481 aaaaaa
//
