LOCUS       NM_054040               9714 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus TUB like protein 4 (Tulp4), transcript variant 1,
            mRNA.
ACCESSION   NM_054040 XM_909108
VERSION     NM_054040.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Rybiczka-Tesulov,M., Garritsen,O., Veno,M.T., Wieg,L., Dijk,R.V.,
            Rahimi,K., Gomes-Duarte,A., Wit,M., van de Haar,L.L., Michels,L.,
            van Kronenburg,N.C.H., van der Meer,C., Kjems,J., Vangoor,V.R. and
            Pasterkamp,R.J.
  TITLE     Circular RNAs regulate neuron size and migration of midbrain
            dopamine neurons during development
  JOURNAL   Nat Commun 15 (1), 6773 (2024)
   PUBMED   39117691
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Giusti,S.A., Pino,N.S., Pannunzio,C., Ogando,M.B., Armando,N.G.,
            Garrett,L., Zimprich,A., Becker,L., Gimeno,M.L., Lukin,J.,
            Merino,F.L., Pardi,M.B., Pedroncini,O., Di Mauro,G.C., Durner,V.G.,
            Fuchs,H., de Angelis,M.H., Patop,I.L., Turck,C.W., Deussing,J.M.,
            Vogt Weisenhorn,D.M., Jahn,O., Kadener,S., Holter,S.M., Brose,N.,
            Giesert,F., Wurst,W., Marin-Burgin,A. and Refojo,D.
  TITLE     A brain-enriched circular RNA controls excitatory neurotransmission
            and restricts sensitivity to aversive stimuli
  JOURNAL   Sci Adv 10 (21), eadj8769 (2024)
   PUBMED   38787942
REFERENCE   3  (bases 1 to 9714)
  AUTHORS   Jager,R., Geyer,S.H., Kavirayani,A., Kiss,M.G., Waltenberger,E.,
            Rulicke,T., Binder,C.J., Weninger,W.J. and Kralovics,R.
  TITLE     Effects of Tulp4 deficiency on murine embryonic development and
            adult phenotype
  JOURNAL   Microsc Res Tech 87 (4), 854-866 (2024)
   PUBMED   38115643
  REMARK    GeneRIF: Effects of Tulp4 deficiency on murine embryonic
            development and adult phenotype.
REFERENCE   4  (bases 1 to 9714)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 9714)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9714)
  AUTHORS   Gurdziel,K., Vogt,K.R., Walton,K.D., Schneider,G.K. and
            Gumucio,D.L.
  TITLE     Transcriptome of the inner circular smooth muscle of the developing
            mouse intestine: Evidence for regulation of visceral smooth muscle
            genes by the hedgehog target gene, cJun
  JOURNAL   Dev Dyn 245 (5), 614-626 (2016)
   PUBMED   26930384
REFERENCE   7  (bases 1 to 9714)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   8  (bases 1 to 9714)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   9  (bases 1 to 9714)
  AUTHORS   Li,Q.Z., Wang,C.Y., Shi,J.D., Ruan,Q.G., Eckenrode,S.,
            Davoodi-Semiromi,A., Kukar,T., Gu,Y., Lian,W., Wu,D. and She,J.X.
  TITLE     Molecular cloning and characterization of the mouse and human TUSP
            gene, a novel member of the tubby superfamily
  JOURNAL   Gene 273 (2), 275-284 (2001)
   PUBMED   11595174
REFERENCE   10 (bases 1 to 9714)
  AUTHORS   Planchart,A. and Schimenti,J.C.
  TITLE     Experimental and computational approaches yield a high-resolution,
            1-Mb physical map of the region harboring the mouse t haplotype
            sterility factor, tcs1
  JOURNAL   Mamm Genome 12 (8), 668-670 (2001)
   PUBMED   11471064
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK173165.1, AF219945.1, AK039439.1, BU510078.1, CX219752.1,
            CN669880.1, AK004288.1 and BP764413.1.
            
            On Sep 7, 2007 this sequence version replaced NM_054040.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF219945.1, BC138024.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-596               AK173165.1         1-596
            597-5750            AF219945.1         1-5154
            5751-6989           AK039439.1         1633-2871
            6990-7557           BU510078.1         261-828
            7558-8226           CX219752.1         43-711
            8227-8630           CN669880.1         118-521
            8631-9681           AK004288.1         203-1253
            9682-9714           BP764413.1         29-61               c
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 3.72 cM"
     gene            1..9714
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /note="TUB like protein 4"
                     /db_xref="GeneID:68842"
                     /db_xref="MGI:MGI:1916092"
     exon            1..1009
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     CDS             758..5401
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /note="isoform a is encoded by transcript variant 1; tubby
                     super-family protein; tubby-related protein 4; tubby
                     superfamily protein; tubby like protein 4"
                     /codon_start=1
                     /product="tubby-related protein 4 isoform a"
                     /protein_id="NP_473381.1"
                     /db_xref="CCDS:CCDS28370.1"
                     /db_xref="GeneID:68842"
                     /db_xref="MGI:MGI:1916092"
                     /translation="MYAAVEHGPVLCSDSNILCLSWKGRVPKSEKEKPVCRRRYYEEG
                     WLATGNGRGVVGVTFTSSHCRRDRSTPQRINFNLRGHNSEVVLVRWNEPYQKLATCDA
                     DGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHW
                     SSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHVLLHESDGILSMSWN
                     YPIFLVEDSSESDTDSDDYSPPQDGPAAYPIPVQNTKPLLTVSFTSGDISLMNNYDDL
                     SPTVIRSGLKEVVAQWCTQGDLLAVAGMEQQAQLSELPNGPLLKSAMVKFYNVRGEHI
                     FTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLRED
                     KDVNKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDD
                     PEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDSKSDEIYGNSLISTVI
                     DSCNCSDSSDIELSDDWAAKKSPKISRSSKSPKLPRISIEARKSPKLPRAAQEISRSP
                     RLPMRKPSMGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAV
                     IYKTSLLHLQPRQMTIYLPEVRKISMDYINLPVFNPNVFSEDEDDLPVTGASGVPENN
                     PPCTVNIPIAPIHSSAQALSPTQSIGLVQSLLANQNVQLDVLTNQTTAVGSAEHAGDA
                     ATQYPVSSRYSNPGQVIFGGVEMGRIIQNPPQLPLPPPPPPPPQAPMQLSAVDHGDRD
                     HEHLQKSAKALRPVPQLAAEGDAVVFSAPQEVQVAKMNPPPPYPGTIPAAPTTAAPPP
                     PLPPPQPPVDVCLKKGDFSLYPTAAHYQPPLGYERITTFDSSGNVEEVCRPRTRMLCS
                     QNTYTLPGPGSSATLRLTATEKKVPQPCTSATLNRLTVPRYSIPTGDPPPYPEIASQL
                     AQGRSAAQRLDNSLIHATLRRNNREVALKMAQLADSSRAPLQPLAKPKGGAAGAVAQL
                     PARPPPALYTCSQCSGAGPSSQSGAALAHAISTSPLASQSSYNLLSPPDTSRDRTDYV
                     NSAFTEDEALSQHCQLEKPLRHPPLPEAAVTMKRPPPYQWDPMLGEDVWVPQERTAQP
                     TVPNPLKLSPLMLGQGQHLDVARVPFVPPKSPSSPTATFPTGYGMGMPYPGSYNNPSL
                     PGVQAPCSPKDALSQAQFAQQESAVVLQPAYPPSLSYCTLPPTYPGSSTCSSVQLPPI
                     ALHPWNSYSTCPPMQNTQGTLPPKPHLVVEKPLVSPPPAELQSHMGTEVMVETADNFQ
                     EVLSLTESPVPQRTEKFGKKNRKRLDSRAEEGSVQAITEGKVKKDARTLSDFNSLISS
                     PRLGREKKKVKSQKDQLKSKKLNKTNEFQDSSESEPELFISGDELMNQSQGSKKGWKS
                     KRSLRTASELEEFKCRKASEKEDGRLGSQGFVYVMANKQPLWNEATQVYQLDFGGRVT
                     QESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK"
     exon            1010..1138
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1300
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1481
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1616
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1783
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..2008
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2243
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2388
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2533
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2627
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..2771
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..5284
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     exon            5285..9696
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9664..9669
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /note="hexamer: AATAAA"
     polyA_site      9689
                     /gene="Tulp4"
                     /gene_synonym="1110057P05Rik; 2210038L17Rik; Tusp"
                     /note="major polyA site"
ORIGIN      
        1 ccatggagga gtcattctag gaagccctgt gttcctaggg acacagggcc aggctttgag
       61 acaggaagct tctggctgtg agcagtgggg gaaagagtga ttttcttgtt aaagctttga
      121 ccattgtctg catgagctct ggtgtgactt tgcacgttag tgtgcctttc cccttatgca
      181 accttttcca gcttacagca gaaacttgcc gagttcagaa aacgtgccag agggtggctt
      241 cagagggaag atgatcttgt gtgatcagtc tctgcacttg aactattgaa tagagaaatc
      301 cagctagagg aattcttacc gccttaagtt acttgaaatc tatgtgtttg taaccctttg
      361 tctctggaat tacattacaa aaaaaactgg aatctcaggc tgagaataac gaggctgagt
      421 aaaagcgaag agaactgcct cttcatcatc acttactaac agctctttct ccaaaggatt
      481 ggtgtggttt cccgctaaga acttgaaaat gagaacggac cctgtgtatt tttaggcatt
      541 acctttcttc gccgactgac gtcttttata gaggagtttt ttcactatgc atttggtgga
      601 gctttataag ctattgacct aattggactc tagatcagtt gtaactaaag gagaaaaaaa
      661 caaaccaacg gaacccaacc acaaaaataa gccaataaaa gaacttggtt tgaaattcct
      721 cagtactttt aaagtgaaat acttcattga aaaaagtatg tatgcagcag tggaacatgg
      781 gcctgtgctt tgcagcgatt ccaacatcct ctgcctgtcc tggaaggggc gtgttcccaa
      841 gagtgagaag gagaaacctg tgtgcagaag gcgctactat gaagagggat ggttggccac
      901 aggcaatggg cgaggtgtgg tgggagtgac tttcacctcg agtcactgtc gcagagatag
      961 gagtacacca cagagaataa acttcaacct gcgaggccac aacagtgagg ttgtgctggt
     1021 gagatggaat gagccatacc agaaactggc cacatgtgat gcagacggag gaatctttgt
     1081 gtggattcaa tatgaaggca ggtggtctgt ggaactggtc aatgaccgtg gggctcaggt
     1141 gagtgatttc acgtggagcc atgatgggac tcaagctctc atttcatatc gagatgggtt
     1201 tgtcctggtt ggttctgtca gtggacaaag acactggtca tctgaaatca acctggagag
     1261 ccagatcacc tgtggtatat ggactcccga tgaccagcag gtgttgtttg gcacagcaga
     1321 tgggcaggtg atcgtcatgg actgccacgg cagaatgcta gcccatgtcc tcctgcatga
     1381 atctgatggc atcctcagca tgtcctggaa ttaccccatc ttcctggtgg aggacagcag
     1441 tgagagtgac actgactcgg atgattattc tcctccccaa gatgggcccg cggcttaccc
     1501 catccctgtg cagaacacca agccgctgct caccgtcagc ttcacctccg gagacatcag
     1561 cttaatgaac aactatgacg acttgtctcc cactgtcatc cgttcagggc tgaaagaggt
     1621 ggtggcccag tggtgtacgc agggagactt actggcagtt gctgggatgg agcagcaggc
     1681 acagctcagc gaactgccca atggtcctct tctgaagagt gccatggtca agttctacaa
     1741 tgttagagga gagcacattt tcactctgga tacacttgta cagcgcccta tcatctctat
     1801 ctgttggggc catcgggact ctaggctgct tatggcttca ggaccagccc tgtatgtggt
     1861 tcgtgtggaa caccgagtgt ccagtctcca gctgttgtgc caacaggcca tcgccagcac
     1921 tcttcgagaa gacaaggatg ttaacaagct gaccttgccc cctcgtctct gttcctacct
     1981 ctccactgcc ttcattccca ctattaagcc cccgattcca gaccccaaca acatgcgaga
     2041 ctttgtcagc tacccctcag cgggcaatga acggctgcac tgcaccatga agcgcacaga
     2101 ggatgaccca gaggtcggcg ggccctgcta cacactctac ttggagtacc tgggagggct
     2161 cgtgcccatt ctcaagggga ggcgcatcag caaactgcgg ccagagtttg tcattatgga
     2221 ccctcggaca gacagcaaat cagatgagat ctatgggaac agcttaattt ctactgtgat
     2281 tgacagctgc aactgctcag actccagtga tatcgaactg agtgatgatt gggctgccaa
     2341 gaagtctccc aaaatctcca gatctagcaa atcgcccaaa ctcccaagga tcagcattga
     2401 ggcccgaaag tcacccaagc ttccacgggc tgctcaagag atttctcggt ctccccgatt
     2461 gcccatgcgc aagccctcca tgggctcgcc cagcctgaca cggagagagt ttccttttga
     2521 agacatcact cagcacaact atcttgctca ggtcacatcc aatatctggg gaaccaagtt
     2581 taagattgtg ggcttggctg cattcctacc aaccaacctc ggtgcagtaa tctataaaac
     2641 cagcctgctg catctccagc ctcggcagat gaccatttat ctcccggaag ttaggaagat
     2701 ttccatggac tatattaacc tgcctgtctt caacccaaac gttttcagtg aagatgagga
     2761 tgacttacca gttacaggag catcaggtgt ccctgaaaac aacccgccct gtacagtgaa
     2821 catccctatt gcaccaatcc acagctctgc tcaagctctg tctcccaccc agagcatagg
     2881 gctggtgcag tccctgctgg ccaatcaaaa tgtgcagttg gatgtcttaa ccaatcagac
     2941 aacagctgta gggtcagcag agcatgcagg tgatgctgct acccagtacc cagtctccag
     3001 tcggtactcc aatcctggac aggtgatttt tggaggtgta gaaatgggcc gcatcattca
     3061 gaatcctcct caattgcctt tgccaccacc gccaccacca ccaccacagg cgcctatgca
     3121 gctgtctgcg gtggaccatg gagatcggga ccatgagcac ctgcagaagt cagccaaggc
     3181 cctgcgccca gtgcctcagc tggctgccga gggggatgcg gtagttttca gtgccccgca
     3241 ggaggtccag gtggcgaaga tgaatccccc acccccgtat ccaggaacta tccctgctgc
     3301 ccccaccacg gctgcacccc caccccctct gccaccacca cagcccccag tggatgtgtg
     3361 tttgaagaag ggtgacttct cactgtaccc tacagcagca cattaccagc ctccactagg
     3421 ctatgagagg attaccacct ttgacagcag tggcaatgtg gaagaggtgt gccggcctcg
     3481 gactcggatg ctctgttccc agaacaccta cactctccct ggcccaggca gttctgccac
     3541 cttgagactc acagccactg agaagaaggt cccccaaccc tgcaccagtg cgactctgaa
     3601 ccgactaact gtccctcgat actccatccc cacaggggac ccacctcctt accctgaaat
     3661 tgctagccag ctggcccagg ggcggagtgc cgctcaaagg ctggacaaca gtctcatcca
     3721 tgccacccta cgaaggaaca accgagaggt ggcacttaag atggcccagc tggcagacag
     3781 ctcccgtgcc ccactgcagc ccctagccaa gcctaagggg ggtgctgctg gggcagtggc
     3841 acagctccca gcaagacctc cacctgccct atacacctgc agccagtgca gtggagcagg
     3901 gcccagctcc cagtcaggtg cagccctggc ccatgctatc agcacctccc cactggcctc
     3961 ccagtcctcc tataatcttc taagccctcc tgacacctcc cgtgatcgta ctgactatgt
     4021 caactcagcc ttcaccgagg atgaggccct gtctcagcac tgtcagctcg agaagcctct
     4081 gaggcacccc ccacttcctg aagctgctgt caccatgaag cgacctcccc cttaccagtg
     4141 ggaccccatg ctgggtgagg acgtttgggt tccccaggaa aggacagcac aacctacagt
     4201 gcccaacccc ttaaaactgt cccccctcat gctagggcaa ggccagcacc tggatgtggc
     4261 tcgagtgccc tttgtgcctc ccaagtctcc gtccagccct actgccacct tcccgacagg
     4321 ctatgggatg ggaatgccat atccaggcag ctataacaac ccctctttgc ctggagtaca
     4381 agctccatgc tcccctaagg atgccctgtc ccaggcacag tttgcacaac aggagtctgc
     4441 tgtggtactt cagccagcat acccacccag cctttcctac tgcaccttgc cccccacata
     4501 cccaggaagc agcacctgct ccagtgtaca gctgccgccc atcgccttgc acccttggaa
     4561 ctcctacagc acctgcccgc ccatgcagaa cacccagggc actcttcccc ccaagcccca
     4621 cttagtggtg gagaagcctc ttgtgtcccc tccacctgcc gagctccaaa gccacatggg
     4681 cacagaggtg atggtagaga ctgcagacaa tttccaagaa gtcctctctc tgacagaaag
     4741 ccctgtaccg cagagaacgg aaaaattcgg aaagaagaac cggaagcgcc tggatagccg
     4801 agcagaggaa ggaagtgttc aggccatcac tgagggcaaa gtgaaaaagg atgctaggac
     4861 tttgagtgac tttaattctc tcatctccag cccccgccta gggagagaga agaagaaagt
     4921 gaaaagtcag aaagaccaac tgaagtcaaa gaagttgaac aagacaaacg agttccagga
     4981 cagctcagag agtgagcccg agctgttcat cagcggggat gagctgatga accaaagcca
     5041 gggcagcaag aaggggtgga aaagcaagag gagcctgcgg accgccagtg agctggagga
     5101 attcaagtgc cgcaaagcca gtgagaagga agatgggcgg ctgggcagcc agggcttcgt
     5161 gtacgtgatg gccaacaagc agccactgtg gaacgaagcc acacaggtgt accagctgga
     5221 cttcggagga agggtgaccc aggagtctgc caagaatttc cagattgagt tggaaggacg
     5281 acaggtgatg cagtttggac ggattgatgg caacgcatac atcctagact tccagtaccc
     5341 cttctcagct gttcaggctt ttgctgttgc cctggccaat gtgactcagc gcctaaaatg
     5401 aagagacaga tgcaggagta gacatggcag gagcctttgg aggcggagct catactgcta
     5461 ctacctgaga agaacagggg aagccagcag gccaggaggg tgctggtctg taggcatggc
     5521 ccggtcgttc acttagcctt tattgtctct tttcttttgc tagagtggtg tttggaagtg
     5581 aagaatttga ggtaccttgt gttcttgaag gcggtgtagc ttggatatag agtgtactgc
     5641 atttacttct catggccagg accaagggcc tttgcagaga agataggttg ctcggccttg
     5701 ctcttctttt aaacataggg agtgttagtg ttcgttcttc atgtcatctg ccctttattc
     5761 caacattcac tcatgaaaaa aaaatttttt aaacacaacc aaaactctgg aagagtcagc
     5821 ttgtactggg ttgtggagga cagcatttca ttcacagatg tgtgctgctc actaaaaggt
     5881 acctggatga atgagggata catatgcaaa tgccttgtgc tctgcttagg taaaccacca
     5941 tagatgggta ctgccaccat tgtcaacgtg atttgatagg cagtgtgtga gggaagagag
     6001 actgcccatg gacacaggct cacatgcaga cagacaactg gatgagctga tagtgtggct
     6061 catgcaaggg tgattttcca aggatggtag ttggaaaccc cactggtttc ttgtgtcaga
     6121 agtaatccta tctgactttg aaagaaggta agagacttca gtcagtgggc cgatccaggc
     6181 tggggtggag ctgggtttac atcatagtca ttagactgct cactcaccag atacctccct
     6241 ccacttgtca agaacctgcc ttggctctcc ttcccccttt gtttgactct ggagcatttt
     6301 actgtagacg tctttctctt tagatcctat tgctctgtct tttctaaacc gtgaatgaga
     6361 cttgatttgc aactacacct ttccttccct tgtccacatt aaaattcagt tgttttgaat
     6421 gctttccagc agttataagt gtaatcaaaa atatgtgtgc atttcctagc atactaaaag
     6481 atagagccat agtcacatct ttggggaaaa caccacaata tgctgtaagg gggaaaaaaa
     6541 actgtttttg ggtgcataga ctgtactaaa agattgacca caactatggg agagccttgt
     6601 taagactgtc tgtgctttat tcattctgcc tgtagcatgt ttgcaaaatt ttttctcgtg
     6661 ggaacacaca cacccaggaa aacacccaga ccttgggagt aaggcccagt tctcgctgtc
     6721 gctgcaacct gtgctgcttg ttcccagagc gcaagagctt taacttttga cttgggtctt
     6781 atttgaaagc tgacaagtgg aagatgctaa ggaaattctt ttcaccttaa atgttgattt
     6841 agtttcctcc tctttttcct cttcctcctc ctccttgtgt ttggtttaaa aataatgaaa
     6901 gaaactgtct gattccttta gctgagaaaa attaaattac tcatcatttt ttcctagatt
     6961 ccaaggaact atggacaaga acatctccca gctgatagtg gggttttcca ttttataatt
     7021 taaaatatga gagctgattc ttaggtgggc cagttgtccc ggaacaagtt gagcagtatg
     7081 ttcagagtta gtgtgtttac gtaagtccct gtagcttgat aaggcatcca gtgcttagca
     7141 ttagctgtgc aatactgttg tcgcctctct gtgtatggaa taatttaaat gcagttttct
     7201 ttgttttgtt gtctaagaga tagttattta tcttgctctt tatagtattc ttgggaacta
     7261 ttttgttatt atgtattggt gatttatgcc cattttatta tttatttaga aaaatagtac
     7321 ctttgtaatg ccttgtttgg tggcaatata ttttgctgca aaaaataaag agaatatgac
     7381 agaaggtttt tgtgggggac ggggaggcta ggtgctttgc aactttcttg agagttggag
     7441 aaaatcttat tgatccccta aaaaaataaa ttcactcttt atctcagtat tgattatatg
     7501 caaaataggc aatatctttg agatagcata ccctaagaca gtatttctta ttttgaaaat
     7561 gttaatttaa aataaaatgt gataagacac aggaagggca agacatctga gataagagtc
     7621 tcaaaagagt cccttagtaa atgatcttca agttttaggg gttttttttt ttgttgttgt
     7681 ttgttttgtt tgtttgtttt gtttttttta gttaaaattt accttttcca gactgcaaaa
     7741 gttatactta caactttgct aaataagaac atccctaaaa gatactcttt gtctgtggag
     7801 atgcaggctt ctggccttga gttgcttgac ctgtgagctg caggaacagc agccactcac
     7861 cagcctcctc ccccaggtgg agagtgagtc tcaggttcca catgggcaga aagctgctgg
     7921 ctgcagcagt tgtggcatcg tatcttttaa gataatggct tcttccagtc aatatcactt
     7981 gattaaattc tcctcaaatg aagactttta agtaacagaa ttttgggtac ttcatggtta
     8041 ttaatagcaa tttgtgaatg tcccaatgtc tcattggata ctgaaattaa aaacagtcaa
     8101 ctatatagag acccatagga aaagagtata ttaaccaaca tgaaaactag tgttagattt
     8161 atttggctct agtatcatca aaacaaaagt tatgggtgaa tatacttatt tgaatgtata
     8221 gcctgagaaa aactgggacc accacagcta gaaatactcg gctctaatga gcatacaaag
     8281 cgaaagcttt tgcatgttgg ttttgtccat ccagagactg gccttctgtg taatactcac
     8341 tctgtagtca aaggccaggg actccaggtc cacttgtcgt gccacgggga taggggtgac
     8401 tttggccagg cccctagggt gcctggcctc cttttgtcca tctcttaaat gaaaagtcat
     8461 acctggagaa gcttaaacac tgcattctgg ctctggtagc ctgagagctc agaattcagc
     8521 agcttgtctt ccctttcacg agggtgggag tatgtgtcaa aacgtggcac ctgtgtagac
     8581 aaaagttttc atgagtctgt aagctgtcta ttgtctcctg aaaagaaacc agaagttttc
     8641 ccctaaatgt gtttagaatg cttattctaa taaaatgatg gaagcatcaa atccaagtgt
     8701 agtggaagtc cccaaggagt ccccaaggag tggggggtcc tcaagtaaaa caaggcactg
     8761 tgtggctgta aaactgggtg tgggttgccg cctttggtgt gctgtcctgt gagctgggga
     8821 caggttggcc acagtagctg ggtttgagct ggcatttgct cctgggagtt tttggtaaca
     8881 tacagttcag aagactacac cgctgtgtat cgcctcatct ctggcagatg aactctggag
     8941 gattgtgact gacttggttt ccattttctt cagctttgtg cagttctcct gtgactttgt
     9001 atccgtgttt tctgtttaca agtcaatttt aaggggaagg gtaggataag atctagttta
     9061 ttgtagatat tctttctcgt gttgtagaaa agcatgggtt atacatttga gtggaaacgg
     9121 cactgtatta tttaccaaag gtgtattgtt tgcatttgtt tataaatgca ttattttagt
     9181 actgtaaatt tgggcataat ttccgagttt actactattg ctactgctgg catctgtctt
     9241 tctccttctc tgtcatcttt tttatcccca taaatgtgca atgtggggtg catatgtcac
     9301 agaccaaaca agaccaccag catgttcttg tccacacttt gggaacagcg tgcactgttt
     9361 catacacatc ggtccctgca gtgttggttt cttcatttcc tttgaaaagt cagcattttg
     9421 ctttaacctt ccagacaaga tctgttgaga gtcaccattg catctcaatg agctctccct
     9481 tatttgccta tctttgtatg tattttgtat actagattgt ccaggaagta ttctagaagg
     9541 gagtaatggt ttgcattcaa aatgatgtaa ttttccaaat tattttgcct ttaaaattga
     9601 aatgggttaa tctcattttt ttcttgatga tttgaaattg taagatttgt ccagctattg
     9661 cttaataaaa ttttgcagat caaaaaatat caattaaaaa aaaaaaaaaa aaaa
//
