LOCUS       NM_146190               3716 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus tubulin, gamma complex component 5 (Tubgcp5),
            transcript variant 1, mRNA.
ACCESSION   NM_146190
VERSION     NM_146190.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Remy,M.H., Merdes,A. and Gregory-Pauron,L.
  TITLE     Assembly of gamma-tubulin ring complexes: implications for cell
            biology and disease
  JOURNAL   Prog Mol Biol Transl Sci 117, 511-530 (2013)
   PUBMED   23663981
  REMARK    Review article
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Kollman,J.M., Merdes,A., Mourey,L. and Agard,D.A.
  TITLE     Microtubule nucleation by gamma-tubulin complexes
  JOURNAL   Nat Rev Mol Cell Biol 12 (11), 709-721 (2011)
   PUBMED   21993292
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3716)
  AUTHORS   Stefan,M., Claiborn,K.C., Stasiek,E., Chai,J.H., Ohta,T.,
            Longnecker,R., Greally,J.M. and Nicholls,R.D.
  TITLE     Genetic mapping of putative Chrna7 and Luzp2 neuronal
            transcriptional enhancers due to impact of a transgene-insertion
            and 6.8 Mb deletion in a mouse model of Prader-Willi and Angelman
            syndromes
  JOURNAL   BMC Genomics 6, 157 (2005)
   PUBMED   16280085
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3716)
  AUTHORS   Chai,J.H., Locke,D.P., Greally,J.M., Knoll,J.H., Ohta,T., Dunai,J.,
            Yavor,A., Eichler,E.E. and Nicholls,R.D.
  TITLE     Identification of four highly conserved genes between breakpoint
            hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes
            deletion region that have undergone evolutionary transposition
            mediated by flanking duplicons
  JOURNAL   Am J Hum Genet 73 (4), 898-925 (2003)
   PUBMED   14508708
  REMARK    GeneRIF: indentification on mouse chromosome 7C
REFERENCE   5  (bases 1 to 3716)
  AUTHORS   Jeng,R. and Stearns,T.
  TITLE     Gamma-tubulin complexes: size does matter
  JOURNAL   Trends Cell Biol 9 (9), 339-342 (1999)
   PUBMED   10461186
  REMARK    Review article
REFERENCE   6  (bases 1 to 3716)
  AUTHORS   Wiese,C. and Zheng,Y.
  TITLE     Gamma-tubulin complexes and their interaction with
            microtubule-organizing centers
  JOURNAL   Curr Opin Struct Biol 9 (2), 250-259 (1999)
   PUBMED   10322210
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160534.5, BB859898.1, AK044887.1, AK051326.1 and AI608079.1.
            
            On Feb 16, 2018 this sequence version replaced NM_146190.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051326.1, AK032031.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AC160534.5         31494-31516         c
            24-49               BB859898.1         5-30
            50-2997             AK044887.1         11-2958
            2998-3704           AK051326.1         2970-3676
            3705-3716           AI608079.1         359-370
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 33.42 cM"
     gene            1..3716
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /note="tubulin, gamma complex component 5"
                     /db_xref="GeneID:233276"
                     /db_xref="MGI:MGI:2178836"
     exon            1..216
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..3145
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /note="isoform 1 is encoded by transcript variant 1;
                     gamma-tubulin complex component 5; tubulin, gamma complex
                     associated protein 5"
                     /codon_start=1
                     /product="gamma-tubulin complex component 5 isoform 1"
                     /protein_id="NP_666302.2"
                     /db_xref="CCDS:CCDS21314.1"
                     /db_xref="GeneID:233276"
                     /db_xref="MGI:MGI:2178836"
                     /translation="MARPKSSGSRMDRQLEHDVCELVRQVTGLQDEADPNFQLALDFV
                     WSNFRFHRFLDVNSHKVEKTIEGIYEKFTIHSDLNKAASWKRLTKEFLNASLPSTEKI
                     KTDAHYSILSLLLCLSDSPSNSNYVETPREKEVEKKDDFDWGKYLMEGEEIGLGPNID
                     TPNWSEDSDDEDAQQPLSREDSGIQVDRTPLEEQDHNRKGGPQVCWEDEPDSRSWLEQ
                     HVVHQYWTTRRFRIPHSAHLHSNLAAVWDQHLYSSDPLYVPDDRVVVTETQVIRETLW
                     LLSGVKKMFIFQLIDGKVTVRNNIIVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEV
                     MGHSSESLPPGNGPIPKKQPDAPFRTYQAFMWALYKYFINFKEELTDIEKCVISSDTT
                     ITLAIVVNKLAPRLAQLKVLDKVFSTGVAEVPPDTRNVVRASHLLNTLYKAILEYDNV
                     GEASEQTVSLLFSLWVETVRPYLQTVDEWIVHGHLWDGAREFIIQRNKNVPVNHRDFW
                     YATYTLYSVSEKTENEDKVSDSASASSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSM
                     QLLKNLNCAEGPACQAAARDAERKSLYTLFLESIQLRLQHGEDSAPHIVNEDQTTKEN
                     LIKMQSIAERHLELDDIHDPLLAINFARLYLEQSDFHEKFAGGDICVDRSSESVTCQT
                     FELTLRSCLYPHIDKQYLHCCGNLMQTLKRDFRLVEYLQAMRNFFLMEGGDTMYDFYT
                     SIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSLRLSISFENVDTTKKKLPVHILDG
                     LTLSYKVPWPVDIVISVECQKIYNQVFLLLLQIKWAKYSLDVLLFGELGNAAERSQAK
                     EDIPRDQDTPSQFGPPKESLRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQ
                     HQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNLALMFAEGWQAGL
                     GAWQMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAGMEQS"
     exon            217..270
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            271..379
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            380..476
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            477..556
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            557..692
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            693..807
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            808..897
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            898..991
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1238
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1441
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1557
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1826
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..2025
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2214
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2397
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2482
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2483..2603
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2782
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2908
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2909..2997
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3098
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3716
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3681..3686
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /note="hexamer: AATAAA"
     polyA_site      3704
                     /gene="Tubgcp5"
                     /gene_synonym="B130010C12Rik; GCP5; mKIAA1899"
                     /note="major polyA site"
ORIGIN      
        1 gccgggacgg gcggaactct cgcgagaaca gtcgccactt agcagagatc ctgataggta
       61 cgccgcggca atggcccggc ctaagtcgtc cgggagccgg atggacagac agctggagca
      121 tgatgtgtgc gagctcgtcc ggcaggttac ggggctgcag gacgaggcgg accccaactt
      181 ccagctggcc ctggacttcg tctggtccaa cttcagattt catcgtttct tggatgtcaa
      241 cagtcacaaa gtagaaaaaa caatagaagg aatttatgaa aaattcacca ttcattctga
      301 tctcaacaaa gctgctagtt ggaagagatt aactaaggag ttcctaaatg catctcttcc
      361 cagcacagag aaaataaaga cagatgcaca ttattccata ctgtcactcc ttctgtgtct
      421 gtctgattct ccttcaaaca gcaattatgt ggaaacaccg agagagaaag aagtggaaaa
      481 gaaggatgat tttgactggg ggaagtacct gatggaaggt gaagaaattg gccttggtcc
      541 aaatattgat acgccgaatt ggtctgaaga cagtgatgat gaagatgcac agcagccctt
      601 gagcagagaa gactctggga tacaggtaga caggacccca ttagaagagc aagaccacaa
      661 cagaaaaggg gggcctcaag tctgctggga agatgagcca gatagccgga gctggctgga
      721 acagcatgta gttcatcagt actggacaac caggcgcttc cgaattccac acagtgcaca
      781 tctgcactcc aatttagctg ctgtctggga tcaacacctg tacagcagtg accctctgta
      841 cgtgccagat gacagggtgg tggttactga gacacaggtc attcgggaga ccctgtggtt
      901 actttcaggg gtgaaaaaga tgtttatatt tcagttgata gatgggaagg taactgtgag
      961 aaacaatatt attgtaactc acttgacaca tagctgttta cgatctgtgc tggaacaaat
     1021 agcagcgtat ggccaggttg tgtttcggct ccaggagttc attgatgaag tcatggggca
     1081 cagctctgaa agtctaccac cgggaaatgg ccccatacct aagaagcaac ctgacgctcc
     1141 ctttagaacc taccaggcct tcatgtgggc cctctacaaa tacttcatta acttcaagga
     1201 agagcttaca gacattgaga agtgtgttat cagtagtgat acaaccataa cacttgcaat
     1261 agtggtgaac aagttggcac ctcgattggc gcagctaaag gtcctggaca aagtgtttag
     1321 tactggagtg gcagaagttc cacctgatac tcgcaatgtc gtccgggcat ctcacctgct
     1381 taacaccctg tacaaggcca ttcttgaata tgacaatgtt ggcgaagcct ctgaacaaac
     1441 agtctccctc ctgttctctc tctgggtgga aacagtgagg ccctacctac agacggtaga
     1501 tgaatggatc gtccatggac acctatggga cggcgcaagg gagtttatca tacagaggaa
     1561 taaaaacgtt ccagtaaatc acagagactt ctggtatgca acgtacacac tctatagcgt
     1621 atctgagaag acagagaatg aagacaaagt gagtgacagt gccagtgcca gctctggcag
     1681 tgaccagggg ccctcgagca gacagcacac catggtgtcc ttcctgaagc ctgtcctgaa
     1741 gcagatcata atggctggca agtcaatgca gctgctgaag aacctcaact gtgcagaggg
     1801 ccctgcgtgc caggcagcag ccagagatgc agaaagaaaa agcttatata ccctctttct
     1861 ggaatctata cagttacgtc ttcaacatgg agaagattct gctccacaca ttgttaatga
     1921 agatcaaaca accaaagaga accttataaa gatgcagtcc attgctgaaa ggcaccttga
     1981 gctagatgat attcatgacc cactgttggc catcaacttt gcaaggttgt atttggagca
     2041 gagcgacttt cacgagaagt ttgctggtgg tgacatatgc gtggatagat catcagagtc
     2101 tgtgacctgc cagacttttg aattaacact gaggtcttgc ctctatcctc acattgataa
     2161 gcagtatcta cattgctgtg ggaatctcat gcaaacctta aagagagatt tcaggctggt
     2221 ggagtacttg caggccatga ggaatttttt cttgatggaa ggtggagaca ccatgtatga
     2281 cttctatact tcaatttttg ataaaataag agaaaaggaa acctggcaga atgtgtcttt
     2341 tcttaatgtg cagctccaag aagcagtagg acagcgctat cctgaagaca gtttacgttt
     2401 atctatatct tttgaaaatg ttgatacaac taagaagaaa ctacccgttc atatcttaga
     2461 tggtcttacc ctaagctaca aggtcccatg gcctgtggac attgttataa gtgtagaatg
     2521 tcaaaaaatt tataatcagg tgttccttct cctattgcaa ataaaatggg caaaatacag
     2581 cctggatgtt ctactatttg gtgaactggg taatgctgct gaaagatccc aagcaaagga
     2641 agacattcca cgtgatcagg acacaccctc ccagtttgga ccaccaaaag agtcattaag
     2701 acagcagatt catcgcatgt ttctcttaag agtgaagctc atgcactttg tgaatagttt
     2761 acacaactac atcatgacca ggattcttca cagtacaggg ctggagtttc agcatcaagt
     2821 tgaggaagcc aaagatttag atcagttgat taaaattcac tacaggtatt tgtcaaccat
     2881 ccatgatcga tgtctgctga gagaaaaggt cagcttcgtg aaagaagcca tcatgaaagt
     2941 gttgaacttg gcgctcatgt ttgcagaagg ttggcaggca ggcctagggg cttggcaaat
     3001 ggaatctata gagaagatgg aatctgactt taaaaactgc cacatgtttc tggtaaccat
     3061 cttaaataaa gctgtgtgta gaggatcttt tccccacttg gaatctcttg cactgtcgct
     3121 catggctggc atggagcaaa gttaaatatc tctaatttaa caagtacctt agacttcagc
     3181 atcatccatg tacatattca ctgtttgcag ctgagaaata agaatctgtt tatttttatt
     3241 tatttttatt gttaaacctg tctagacaaa tggattgtag gtatttttct gaagattatt
     3301 atcgagtatg tagagttatg tatataatat tgtatatata attgtaaaat atataggagt
     3361 tgtataaaag tagactacta atatttcttt tcttctaaaa gattgactta ttttaatgta
     3421 tatgagtatt ttgcctgctt gtatatatgt acatacctgg tgctcagaag caataaaaga
     3481 acatcagata ccctgtaagt tttacatttc ctcttaccta aaaattgtct tagatttcac
     3541 tatcctggat gtatttttat gtgcatagaa aatcccaaaa gaaaaataac aaccaatatc
     3601 aatatataaa ctgtacttta cttaaggcca gctgttatat atcgattttt ggcatttatt
     3661 agctaaccag taatatctca aataaaagta tgtatatcat cccatttttc taccta
//
