LOCUS       NM_153387               4230 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tubulin, gamma complex component 4 (Tubgcp4),
            transcript variant 1, mRNA.
ACCESSION   NM_153387
VERSION     NM_153387.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Camblor-Perujo,S., Ozer Yildiz,E., Kupper,H., Overhoff,M.,
            Rastogi,S., Bazzi,H. and Kononenko,N.L.
  TITLE     The AP-2 complex interacts with gamma-TuRC and regulates the
            proliferative capacity of neural progenitors
  JOURNAL   Life Sci Alliance 7 (2), e202302029 (2023)
   PUBMED   38086550
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4230)
  AUTHORS   Li,Z., Li,H., Xu,X., Wang,L., Liu,B., Zheng,W., Lian,L., Song,Y.,
            Xia,X., Hou,L., Cheng,H. and Zhou,R.
  TITLE     Haploinsufficiency of GCP4 induces autophagy and leads to
            photoreceptor degeneration due to defective spindle assembly in
            retina
  JOURNAL   Cell Death Differ 27 (2), 556-572 (2020)
   PUBMED   31209365
  REMARK    GeneRIF: Haploinsufficiency of GCP4 induces autophagy and leads to
            photoreceptor degeneration due to defective spindle assembly in
            retina.
REFERENCE   4  (bases 1 to 4230)
  AUTHORS   Xu,X., Shang,D., Cheng,H., Klionsky,D.J. and Zhou,R.
  TITLE     Gene essentiality of Tubgcp4: dosage effect and autophagy
            regulation in retinal photoreceptors
  JOURNAL   Autophagy 15 (10), 1834-1837 (2019)
   PUBMED   31345090
REFERENCE   5  (bases 1 to 4230)
  AUTHORS   Sulimenko,V., Draberova,E., Sulimenko,T., Macurek,L.,
            Richterova,V., Draber,P. and Draber,P.
  TITLE     Regulation of microtubule formation in activated mast cells by
            complexes of gamma-tubulin with Fyn and Syk kinases
  JOURNAL   J Immunol 176 (12), 7243-7253 (2006)
   PUBMED   16751367
  REMARK    GeneRIF: Gamma-tubulin forms complexes with Fyn and Syk kinases and
            other signal transduction molecules in the process of bone
            marrow-derived mast cell activation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845479.8 and AL929059.5.
            
            On Jan 5, 2022 this sequence version replaced NM_153387.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029106.1, AK045305.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               AL845479.8         83233-83513
            282-410             AL845479.8         85600-85728
            411-533             AL845479.8         87382-87504
            534-587             AL845479.8         87813-87866
            588-644             AL845479.8         88570-88626
            645-724             AL845479.8         90712-90791
            725-926             AL845479.8         95531-95732
            927-1092            AL845479.8         96376-96541
            1093-1217           AL845479.8         96744-96868
            1218-1268           AL845479.8         100174-100224
            1269-1374           AL845479.8         100567-100672
            1375-1482           AL845479.8         101404-101511
            1483-1624           AL845479.8         101866-102007
            1625-1802           AL929059.5         3992-4169
            1803-1937           AL929059.5         5701-5835
            1938-2054           AL929059.5         7169-7285
            2055-2194           AL929059.5         7862-8001
            2195-4230           AL929059.5         8494-10529
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..4230
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="tubulin, gamma complex component 4"
                     /db_xref="GeneID:51885"
                     /db_xref="MGI:MGI:1196293"
     exon            1..281
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..2207
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="isoform a is encoded by transcript variant 1; gamma
                     tubulin ring complex protein; gamma-tubulin complex
                     component 4; tubulin, gamma complex associated protein 4"
                     /codon_start=1
                     /product="gamma-tubulin complex component 4 isoform a"
                     /protein_id="NP_700436.1"
                     /db_xref="CCDS:CCDS16637.1"
                     /db_xref="GeneID:51885"
                     /db_xref="MGI:MGI:1196293"
                     /translation="MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNR
                     LCRLGTDYIRFTEFIEQYTGHVQQQDHHPPQQGQGGLHGIYLRAFCTGLDSVLQPYRQ
                     ALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKH
                     SCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGTLSAQL
                     EEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKI
                     LFVGESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRS
                     TVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDV
                     NVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKDHKADATQPREVPSRETSPREAPSSGW
                     AALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSN
                     QTDAVKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLS
                     NLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSS
                     LLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM"
     exon            282..410
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            411..533
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            534..587
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            588..644
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            645..724
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            725..926
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1092
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1217
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1268
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1374
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1482
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1624
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1802
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1937
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2054
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2194
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..4230
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3571..3576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="hexamer: AATAAA"
     polyA_site      3589
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="major polyA site"
     regulatory      4211..4216
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="hexamer: AATAAA"
     polyA_site      4230
                     /gene="Tubgcp4"
                     /gene_synonym="4932441P04Rik; 76p; D2Ertd435e; GCP-4"
                     /note="major polyA site"
ORIGIN      
        1 ctcgcggctc cgcggcggga gcgaggcggt gggcgcctgg gccgcgcgca cttccgggat
       61 gcggcgcgcg gctctgtggc atctcctctc ccggtggact gcggggattc agggcaagag
      121 attccgctgg gtgtgagggg tcgcccggag aggcgctgaa aagaggaggt ggtagatcta
      181 gggactcccg agccgcccta gaaatgattc acgaactgct cttggccctg agtgggtacc
      241 cagggtccat tttcacctgg aacaagcgga gtggcctgca ggtttcccag gattttccat
      301 ttcttcaccc cagtgagact agtgtcctga atcgactctg ccggcttggc acagactaca
      361 ttcgattcac tgagttcatt gaacagtata caggccatgt gcagcaacag gaccaccatc
      421 ctccccagca gggccaaggg ggactgcatg ggatctacct gagagccttc tgcacagggc
      481 tggattcagt tttgcagccg tatcgccaag cactgcttga tttggaacaa gagttcctgg
      541 ctgacccgca tctctccata tcacatgtca attactcctt agatcagttc cagctccttt
      601 tcccctctgt gatggttgta gtggagcaaa ttaaaagcca aaagattcat ggctgtcaaa
      661 tcctggaaac agtttacaaa catagctgtg ggggcttgcc tccagtccga agcgcattag
      721 aaaaaatcct ggctgtgtgc catggagtca tgtataaaca gctctcagcc tggatgctgc
      781 atggactcct tttggatcag catgaagagt tcttcatcaa acagggccca tcttctggta
      841 ctctcagtgc gcagctggaa gaggatgagg aggacctggg cattggaggc ctgacaggaa
      901 aacagctgag agaactgcag gacctgcgcc taattgagga agagaatatg ctggccccat
      961 ctctgaagca gttttcgctg agggtagaga ttttaccatc atacattcca gtgagagttg
     1021 ctgaaaaaat cctctttgtt ggagaatctg tccaaatgtt tgagaatcaa aatgtgaacc
     1081 tgacaaggaa agggtccatc ttgaaaaacc aggaggacac attcgctgcc gagctgcacc
     1141 ggctcaagca gcagccactc ttcagcctgg tggattttga gcaggtggta gatcggattc
     1201 gtagcactgt ggctgagcac ctctggaagc taatggtgga agagtcagat ttactgggtc
     1261 agctgaagat cattaaagac ttttacctgc tgggacgagg agaactgttc caggccttca
     1321 ttgacacagc tcagcacatg ttgaaaacac cacccactgc agtaacagag catgatgtga
     1381 atgtggcctt tcaacagtca gcacacaagg tgttgctaga tgacgacaac cttctccctc
     1441 tgttgcacct gacgattgag tatcatggga aggaccacaa agcagatgcc acccagccaa
     1501 gagaagtgcc ttctcgggag acatcccccc gggaagctcc ttcttccggc tgggctgctc
     1561 tagggctttc ctacaaagta cagtggccac tgcacattct cttcacccca gctgtcttgg
     1621 aaaagtacaa tgttgttttt aagtacttac tgagtgtgcg ccgtgtccaa gctgaactgc
     1681 agcactgctg ggcactccag atgcagcgca aacacctcaa atctaaccag acggacgcag
     1741 tcaagtggcg cctaagaaac cacatggcct tcttggtaga taatcttcag tactatctcc
     1801 aggtagatgt gctggagtct cagttctcac agctgcttca tcagatcaat tctacgaggg
     1861 actttgaaag catccgattg gcacacgacc acttcctaag caatctgctg gcccaatcct
     1921 ttattttgct gaagcctgtg tttcactgcc tgaatgaaat cctagatctc tgtcacagtt
     1981 tttgttcact ggtcagtcag aacctaggtc cactggatga gcgcggagcg gcccagctga
     2041 gcattctcgt gaagggcttt agtcgccagt cttcactcct gttcaagatt ctttccagtg
     2101 ttcggaatca ccagatcaac tcagatttgg ctcagttact gctccggcta gactataata
     2161 aatactatac ccaggctggt gggactctgg gcagttttgg gatgtaaaag aaaactctgg
     2221 ttcaaaaaat gaaacagccc tgccacattc tgatgtctgt aacaaatctc tcaaatcatt
     2281 catcaaatgc ctgcagactg ctgaaagggt cttctagaag aaatgcagag gtataaaaat
     2341 cctccctgcc attcctgtag gggaactggg gtctgatatc cctctgctga ccacacgaag
     2401 gattttgaag acatctgttg ggtgggttca tttgtgtaac cggggtttat tgaacactta
     2461 ctatattcct atggaagata accacagtaa acagtgggtt ggctcatgta agaacggaat
     2521 cacgctcctg aacaggtcag cttctgtgtc accaccaaat gagaaaaccc ttcagagagc
     2581 ctgtgagctt tccctctttc tgtaccttgt aactacacag actatcttta tccttcccta
     2641 aaatttaaga tttgagtatc ctttatctga agaattgagc cagtgagttc taattctttc
     2701 agactagtaa aagggcaatt ctggctaaag aggatagagg tcaaattgtt tagatttaga
     2761 aacaatttag ttttcactgc aacccagttt tcccacctta gacctgccac atgagttaag
     2821 gttttatggt cagatagcct agccaccacg ggctgagaac atactaatgt tctcatcagc
     2881 ataaacaaaa cacttcactg acatctttcc ctccaagctg gacaagaatg aaacatccgg
     2941 gcctcagtct gtagtcactg agtcctctct tcatctacac tgtctaccaa ccactatgaa
     3001 agcctgagcc cgtacttgtc aactacccag gaggattatc ccaccttgtt acctcacctc
     3061 taaaagcaga taacagcctg ctgcttgttt ttgtaaataa agtactattc aaacagccac
     3121 acatactcaa tttagctatt gtcggtgatt gctcacagac aagacaagtt gttgagacag
     3181 acaagtgtgg tcacaaagcc taaaagtatt tactatttgg cactatagaa aaaatgagac
     3241 cgctggcttt atttagagaa tgagaagccg ttcgctaaca gggatgatga tgatgagtgt
     3301 gaggaaggaa taacttccaa cagttgtgac agcttatttt atagaaaacc gtcccagcaa
     3361 atttatagtc actgtccatt cattaacaac tggtcatgtt catgttccca gtagcaggtc
     3421 atctgtcaat aaactcctga tacccagagc tgttcccagt cccactcaac tttagcacta
     3481 ctgtttaccc aggccctcac ctactttaaa ctgtttttgg aagtataaca ttttaggtag
     3541 cctgggataa ccttgacagt gatgccacaa aataaagctc actcctctat ttttatctcc
     3601 ctttgtttgc tgtactgcta aagtgacaag cctgctgaca acttcagaga agatcctatg
     3661 tttatcatgt tctgagatta agctcaaaga aacagatgaa gacatcccag ttactacaac
     3721 caaagagatt caacatttat tttatcataa aagtccagca aataaaacta tgtacaaggt
     3781 ccatgcaaag aatacaatta cacacaagac acacttaaaa cctgattaaa acacaatctc
     3841 cacaatagca gggaataaaa ccagtaagac caagtatctt tagtgagaaa cataatcatg
     3901 tttatatttt ggatgctgct tgaatccaat tctctcccca acaatgaggc actggatcac
     3961 ccattcttgt gataccacag gcagttgcaa ggcttcagca cacttgagca ccgaggctgg
     4021 gcatgagggg tctgtcacca ccacatcaaa tacccctaaa gcaatgtctg taaaaagtag
     4081 gggaagtgaa tgagggaggg acactcagtt tagctcctat taaaacaaga caggtgtgcc
     4141 ttctaacagg tattccctac tctgccaaac caaagccctc tactctaggt actgtatgtt
     4201 cataatctaa aataaaagtt gcaatcacca
//
