LOCUS       NM_146116               1600 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tubulin, beta 4B class IVB (Tubb4b), mRNA.
ACCESSION   NM_146116
VERSION     NM_146116.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Sanzhaeva,U., Boyd-Pratt,H., Bender,P.T.R., Saravanan,T.,
            Rhodes,S.B., Guan,T., Billington,N., Boye,S.E., Cunningham,C.L.,
            Anderson,C.T. and Ramamurthy,V.
  TITLE     TUBB4B is essential for the cytoskeletal architecture of cochlear
            supporting cells and motile cilia development
  JOURNAL   Commun Biol 7 (1), 1146 (2024)
   PUBMED   39277687
  REMARK    GeneRIF: TUBB4B is essential for the cytoskeletal architecture of
            cochlear supporting cells and motile cilia development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Sanzhaeva,U., Wonsettler,N.R., Rhodes,S.B. and Ramamurthy,V.
  TITLE     TUBB4B is essential for the expansion of differentiating
            spermatogonia
  JOURNAL   Sci Rep 14 (1), 20889 (2024)
   PUBMED   39244620
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1600)
  AUTHORS   Dodd,D.O., Mechaussier,S., Yeyati,P.L., McPhie,F., Anderson,J.R.,
            Khoo,C.J., Shoemark,A., Gupta,D.K., Attard,T., Zariwala,M.A.,
            Legendre,M., Bracht,D., Wallmeier,J., Gui,M., Fassad,M.R.,
            Parry,D.A., Tennant,P.A., Meynert,A., Wheway,G., Fares-Taie,L.,
            Black,H.A., Mitri-Frangieh,R., Faucon,C., Kaplan,J., Patel,M.,
            McKie,L., Megaw,R., Gatsogiannis,C., Mohamed,M.A., Aitken,S.,
            Gautier,P., Reinholt,F.R., Hirst,R.A., O'Callaghan,C., Heimdal,K.,
            Bottier,M., Escudier,E., Crowley,S., Descartes,M., Jabs,E.W.,
            Kenia,P., Amiel,J., Bacci,G.M., Calogero,C., Palazzo,V., Tiberi,L.,
            Blumlein,U., Rogers,A., Wambach,J.A., Wegner,D.J., Fulton,A.B.,
            Kenna,M., Rosenfeld,M., Holm,I.A., Quigley,A., Hall,E.A.,
            Murphy,L.C., Cassidy,D.M., von Kriegsheim,A., Papon,J.F.,
            Pasquier,L., Murris,M.S., Chalmers,J.D., Hogg,C., Macleod,K.A.,
            Urquhart,D.S., Unger,S., Aitman,T.J., Amselem,S., Leigh,M.W.,
            Knowles,M.R., Omran,H., Mitchison,H.M., Brown,A., Marsh,J.A.,
            Welburn,J.P.I., Ti,S.C., Horani,A., Rozet,J.M., Perrault,I. and
            Mill,P.
  CONSRTM   Scottish Genomes Partnership16; Genomics England Research
            Consortium45; Undiagnosed Diseases Network46
  TITLE     Ciliopathy patient variants reveal organelle-specific functions for
            TUBB4B in axonemal microtubules
  JOURNAL   Science 384 (6694), eadf5489 (2024)
   PUBMED   38662826
  REMARK    Erratum:[Science. 2024 May 10;384(6696):eadq2178. doi:
            10.1126/science.adq2178.. PMID: 38723101]
REFERENCE   4  (bases 1 to 1600)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 1600)
  AUTHORS   Sewell,M.T., Legue,E. and Liem,K.F. Jr.
  TITLE     Tubb4b is required for multi-ciliogenesis in the mouse
  JOURNAL   Development 151 (1) (2024)
   PUBMED   38031972
  REMARK    GeneRIF: Tubb4b is required for multi-ciliogenesis in the mouse.
REFERENCE   6  (bases 1 to 1600)
  AUTHORS   Miller,L.A., Wert,S.E. and Whitsett,J.A.
  TITLE     Immunolocalization of sonic hedgehog (Shh) in developing mouse lung
  JOURNAL   J Histochem Cytochem 49 (12), 1593-1604 (2001)
   PUBMED   11724907
REFERENCE   7  (bases 1 to 1600)
  AUTHORS   Tichelaar,J.W., Lim,L., Costa,R.H. and Whitsett,J.A.
  TITLE     HNF-3/forkhead homologue-4 influences lung morphogenesis and
            respiratory epithelial cell differentiation in vivo
  JOURNAL   Dev Biol 213 (2), 405-417 (1999)
   PUBMED   10479457
REFERENCE   8  (bases 1 to 1600)
  AUTHORS   Blatt,E.N., Yan,X.H., Wuerffel,M.K., Hamilos,D.L. and Brody,S.L.
  TITLE     Forkhead transcription factor HFH-4 expression is temporally
            related to ciliogenesis
  JOURNAL   Am J Respir Cell Mol Biol 21 (2), 168-176 (1999)
   PUBMED   10423398
REFERENCE   9  (bases 1 to 1600)
  AUTHORS   Tichelaar,J.W., Wert,S.E., Costa,R.H., Kimura,S. and Whitsett,J.A.
  TITLE     HNF-3/forkhead homologue-4 (HFH-4) is expressed in ciliated
            epithelial cells in the developing mouse lung
  JOURNAL   J Histochem Cytochem 47 (6), 823-832 (1999)
   PUBMED   10330459
REFERENCE   10 (bases 1 to 1600)
  AUTHORS   Wang,D., Villasante,A., Lewis,S.A. and Cowan,N.J.
  TITLE     The mammalian beta-tubulin repertoire: hematopoietic expression of
            a novel, heterologous beta-tubulin isotype
  JOURNAL   J Cell Biol 103 (5), 1903-1910 (1986)
   PUBMED   3782288
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732309.9, AK167022.1 and CJ309661.1.
            
            On Apr 23, 2010 this sequence version replaced NM_146116.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083319.1, AK167022.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN02415128
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               AL732309.9         117166-117334       c
            170-1590            AK167022.1         125-1545
            1591-1600           CJ309661.1         421-430
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 17.08 cM"
     gene            1..1600
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /note="tubulin, beta 4B class IVB"
                     /db_xref="GeneID:227613"
                     /db_xref="MGI:MGI:1915472"
     exon            1..169
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1450
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /note="tubulin beta-2C chain; novel tubulin protein;
                     tubulin, beta 2c1; tubulin, beta, 2; tubulin, beta 2C"
                     /codon_start=1
                     /product="tubulin beta-4B chain"
                     /protein_id="NP_666228.1"
                     /db_xref="CCDS:CCDS15753.1"
                     /db_xref="GeneID:227613"
                     /db_xref="MGI:MGI:1915472"
                     /translation="MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL
                     ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWA
                     KGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYP
                     DRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLT
                     TPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR
                     GSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQ
                     NKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK
                     AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEAEEEVA"
     exon            170..278
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..389
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            390..1600
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1576..1581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /note="hexamer: ATTAAA"
     polyA_site      1596
                     /gene="Tubb4b"
                     /gene_synonym="4930542G03Rik; Tubb2c; Tubb2c1"
                     /note="major polyA site"
ORIGIN      
        1 ggcgggtcat aagaggtata taaatgttgg cagagcgtcg gttgtagcac tctgtgcgcc
       61 tgctcctccc gaagtgctcc tcttctacag ctgttccgca gtcgccgccg ccatgaggga
      121 gatcgtgcac ctgcaggctg ggcagtgcgg caaccagatt ggcgccaagt tctgggaggt
      181 aatcagcgac gagcatggca ttgatcccac tggcacttac cacggagata gcgacctcca
      241 gctggagcgc atcaacgtgt actacaacga agccaccggt ggcaagtatg tgccccgcgc
      301 cgtgctcgtg gacttggagc ccggcaccat ggactcggtg cgttcagggc ccttcgggca
      361 gatcttcaga cctgataact tcgtctttgg tcagagtggg gctgggaaca actgggccaa
      421 ggggcactac acagaaggtg cagagctggt tgactcggtg ttggacgttg tgagaaagga
      481 agctgagagc tgtgactgcc tgcagggctt ccagctgacc cactccctgg gtggggggac
      541 tgggtctggg atgggtaccc tccttatcag caagatccgg gaggagtacc cagacagaat
      601 catgaacacc ttcagtgtgg taccttcccc caaggtgtcg gacacagtgg ttgagcccta
      661 caatgccacc ctttcagtcc accagctggt tgaaaacaca gatgagacct attgtattga
      721 taatgaagca ctttatgaca tctgcttcag aaccctaaag ctgaccacac ccacttacgg
      781 tgacctgaac catctagtgt ccgccaccat gagtggggta accacttgcc tgcgattccc
      841 tggccagcta aatgctgacc tgcggaaact ggctgtaaat atggtgccct tccctcgcct
      901 gcacttcttc atgcctggct ttgccccctt gaccagccgg ggcagccagc agtaccgtgc
      961 cctgacagtt cctgagctca cccagcagat gtttgatgcc aagaacatga tggctgcctg
     1021 tgatccaaga catgggcgct acttgactgt ggctgccgtg ttcaggggcc gcatgtctat
     1081 gaaggaggtg gacgaacaga tgcttaatgt ccaaaacaag aacagcagct actttgttga
     1141 gtggatcccc aacaatgtga agacagctgt ctgtgacatt ccacctcggg gcctgaaaat
     1201 gtcggccacc ttcattggca acagcaccgc tattcaggag ctgttcaaac gcatctcaga
     1261 gcagttcaca gccatgttcc gacgcaaggc cttcctacac tggtacacgg gtgaaggcat
     1321 ggatgagatg gagttcactg aggctgagag caacatgaac gacctggtgt ccgagtacca
     1381 gcagtaccag gacgctacag ctgaggaaga gggagagttt gaggaggagg ctgaggagga
     1441 ggtggcttag agctgtctta gtcactaaag catgggagca gtgtgaactc tttattcatt
     1501 cacagcctgt ctgctagcca tgtccactgt gcatttgctg tcctgtgtcc tgacatcact
     1561 tgtacagata ccaccattaa agcaattcat agtgtgtggc
//
