LOCUS       NM_009451               2068 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tubulin, beta 4A class IVA (Tubb4a), mRNA.
ACCESSION   NM_009451
VERSION     NM_009451.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Sewell,M.T., Legue,E. and Liem,K.F. Jr.
  TITLE     Tubb4b is required for multi-ciliogenesis in the mouse
  JOURNAL   Development 151 (1) (2024)
   PUBMED   38031972
REFERENCE   3  (bases 1 to 2068)
  AUTHORS   Liu,H., Zhou,R., Li,S., Dong,J., Fang,Y., Luo,Y., Su,H., Lai,B.,
            Liang,L., Zhang,D., Zhang,Y., Shyy,J.Y., Zhou,B., Yuan,Z. and
            Wang,Y.
  TITLE     Epigenetic repression of Cend1 by lysine-specific demethylase 1 is
            essential for murine heart development
  JOURNAL   iScience 27 (1), 108722 (2023)
   PUBMED   38226173
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2068)
  AUTHORS   Jia,S., Wilbourne,J., Crossen,M.J. and Zhao,F.
  TITLE     Morphogenesis of the female reproductive tract along
            antero-posterior and dorso-ventral axes is dependent on Amhr2+
            mesenchyme in micedagger
  JOURNAL   Biol Reprod 107 (6), 1477-1489 (2022)
   PUBMED   36130202
REFERENCE   5  (bases 1 to 2068)
  AUTHORS   Serra,C.F.H., Liu,H., Qian,J., Mori,M., Lu,J. and Cardoso,W.V.
  TITLE     Prominin 1 and Notch regulate ciliary length and dynamics in
            multiciliated cells of the airway epithelium
  JOURNAL   iScience 25 (8), 104751 (2022)
   PUBMED   35942101
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2068)
  AUTHORS   Cascio,S. and Zaret,K.S.
  TITLE     Hepatocyte differentiation initiates during endodermal-mesenchymal
            interactions prior to liver formation
  JOURNAL   Development 113 (1), 217-225 (1991)
   PUBMED   1764997
REFERENCE   7  (bases 1 to 2068)
  AUTHORS   Johnson,K.R., Disney,J.E., Wyatt,C.R. and Reeves,R.
  TITLE     Expression of mRNAs encoding mammalian chromosomal proteins HMG-I
            and HMG-Y during cellular proliferation
  JOURNAL   Exp Cell Res 187 (1), 69-76 (1990)
   PUBMED   2404776
REFERENCE   8  (bases 1 to 2068)
  AUTHORS   Rienhoff,H.Y. Jr. and Groudine,M.
  TITLE     Regulation of amyloid A gene expression in cultured cells
  JOURNAL   Mol Cell Biol 8 (9), 3710-3716 (1988)
   PUBMED   3221863
REFERENCE   9  (bases 1 to 2068)
  AUTHORS   Schneider,J., Erickson,R.P. and Hanash,S.
  TITLE     Mouse testicular protein variants, including tubulin, detected by
            isoelectrofocusing two-dimensional gel electrophoresis
  JOURNAL   Genet Res 50 (3), 225-233 (1987)
   PUBMED   3443294
REFERENCE   10 (bases 1 to 2068)
  AUTHORS   Fukumoto,M. and Roninson,I.B.
  TITLE     Detection of amplified sequences in mammalian DNA by in-gel
            renaturation and SINE hybridization
  JOURNAL   Somat Cell Mol Genet 12 (6), 611-623 (1986)
   PUBMED   3024334
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC049112.1 and AI837276.1.
            
            On Jul 14, 2018 this sequence version replaced NM_009451.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049112.1, AK013717.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2046              BC049112.1         183-2228
            2047-2068           AI837276.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 29.66 cM"
     gene            1..2068
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /note="tubulin, beta 4A class IVA"
                     /db_xref="GeneID:22153"
                     /db_xref="MGI:MGI:107848"
     exon            1..144
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1422
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /note="tubulin beta-4 chain"
                     /codon_start=1
                     /product="tubulin beta-4A chain"
                     /protein_id="NP_033477.2"
                     /db_xref="CCDS:CCDS28925.1"
                     /db_xref="GeneID:22153"
                     /db_xref="MGI:MGI:107848"
                     /translation="MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL
                     ERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWA
                     KGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFP
                     DRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLT
                     TPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR
                     GSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQ
                     SKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRK
                     AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA"
     exon            145..253
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /inference="alignment:Splign:2.1.0"
     exon            254..364
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /inference="alignment:Splign:2.1.0"
     exon            365..2051
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2025..2030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /note="hexamer: AATAAA"
     polyA_site      2046
                     /gene="Tubb4a"
                     /gene_synonym="M(beta)4; Tubb; Tubb4"
                     /note="major polyA site"
ORIGIN      
        1 agagctccgc agccatctcg tccagagcta gagccacagc ggccggtcga cacccgtcca
       61 tcagacgcca ccagcagcgc caccaccatg cgggaaatcg tgcacctgca agccggtcaa
      121 tgcggtaacc agatcggggc caagttctgg gaggtcatca gtgatgagca cggcatcgac
      181 cccactggga cctatcatgg ggacagtgac ctccagctgg agaggatcaa tgtctactac
      241 aatgaggcca caggtggaaa ctatgttccc agagccgtgc tggtcgacct ggaacccggc
      301 accatggact ctgtccgctc cggccctttt ggccagatct ttcggccaga caactttgta
      361 tttggtcaat ccggagcagg caacaactgg gccaagggtc actacaccga gggcgcggag
      421 ttagtggatg ccgtcctgga cgtggtgcgc aaagaggcgg aaagctgcga ctgtctccag
      481 ggcttccagc tcacccactc gctcggaggt ggcaccggct caggcatggg gaccttgctc
      541 atcagcaaga tccgagagga gtttccagac aggatcatga atacgttcag cgtggtgcca
      601 tcacccaagg tgtctgacac ggtggtggag ccctacaatg ccacactgtc tgtgcatcag
      661 ctggtggaga acactgatga gacctactgc atcgacaacg aggccttgta cgacatctgc
      721 ttccgcacgc tcaagctgac cacgcccacc tacggggacc tcaaccacct cgtgtcagcc
      781 accatgagtg gagtcaccac ctgcctacgt ttcccgggcc agctcaatgc agatctacgc
      841 aagctggctg tgaatatggt gccattcccc cgtctccact tcttcatgcc aggattcgca
      901 cccttgacca gcaggggcag ccagcagtac cgggccctca ccgttcctga gctgacccaa
      961 cagatgttcg atgctaagaa catgatggct gcgtgtgacc cgagacacgg tcgctacctg
     1021 actgtggctg ctgtcttccg gggacggatg tccatgaagg aggtagacga gcagatgtta
     1081 agtgtgcaga gcaagaacag cagttacttc gttgagtgga tccccaacaa tgtcaagaca
     1141 gccgtatgtg acatcccacc ccggggcctg aagatggcag ccaccttcat cggcaacagc
     1201 actgccatcc aggagctgtt caagcgcatc tcggagcagt tcaccgccat gttcagacgc
     1261 aaggccttcc tgcactggta cacgggcgaa ggcatggacg agatggagtt tacggaagca
     1321 gagagcaata tgaacgacct ggtgtccgag taccagcagt accaggatgc cactgctgaa
     1381 gagggcgagt tcgaagagga ggctgaagag gaggtggctt aagtctcctg ccatcactct
     1441 gtccctgggg cccaccagca aagctttgac cctaagcatc acacccctgc acctagttgc
     1501 ctcattccct aggaccccat gagcatcttc accatgaggg ccaagcccag gttgcttcta
     1561 tttgcttcac ctttaactcc taaaccccac tgtctctcca acctgccagg gaagggctct
     1621 tctagttccc atgagcgccc ctcaacacat gtacacacac acacacactc caccttctta
     1681 gatcttgaaa atcctttcct ttatgccctg tcccttcccc agcactcctg aaccattctc
     1741 ctccacctct gacatgccca gatatttctc tagatcttaa cattttcccc agcctccaag
     1801 tcctctcacc ttgccttacc tctgacctca cagctaccca ctcctgaccc agcaccttca
     1861 ctctggctcc cgtaagtccc cgtatgttcc tgcccttgtg ccttagttca gttcctacca
     1921 gagaagtgct gaaggacgag gaaaaagaga ggcctgttgc ctggggtgag agagcccctc
     1981 cctaaaatcc accccaccct gctctcccct gctgaccctc catcaataaa tgagtcattg
     2041 ctttggtgtg gaaaaaaaaa aaaaaaaa
//
