LOCUS       NM_175274               4676 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus tweety family member 3 (Ttyh3), transcript variant 2,
            mRNA.
ACCESSION   NM_175274
VERSION     NM_175274.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Melvin,E., Kalaninova,Z., Shlush,E., Man,P., Giladi,M. and
            Haitin,Y.
  TITLE     TTYH family members form tetrameric complexes at the cell membrane
  JOURNAL   Commun Biol 5 (1), 886 (2022)
   PUBMED   36042377
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4676)
  AUTHORS   Li,B., Hoel,C.M. and Brohawn,S.G.
  TITLE     Structures of tweety homolog proteins TTYH2 and TTYH3 reveal a
            Ca2+-dependent switch from intra- to intermembrane dimerization
  JOURNAL   Nat Commun 12 (1), 6913 (2021)
   PUBMED   34824283
  REMARK    GeneRIF: Structures of tweety homolog proteins TTYH2 and TTYH3
            reveal a Ca(2+)-dependent switch from intra- to intermembrane
            dimerization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4676)
  AUTHORS   Han,Y.E., Kwon,J., Won,J., An,H., Jang,M.W., Woo,J., Lee,J.S.,
            Park,M.G., Yoon,B.E., Lee,S.E., Hwang,E.M., Jung,J.Y., Park,H.,
            Oh,S.J. and Lee,C.J.
  TITLE     Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of
            the Swelling-dependent Volume-regulated Anion Channel (VRACswell)
            in the Brain
  JOURNAL   Exp Neurobiol 28 (2), 183-215 (2019)
   PUBMED   31138989
REFERENCE   5  (bases 1 to 4676)
  AUTHORS   Lumayag,S., Haldin,C.E., Corbett,N.J., Wahlin,K.J., Cowan,C.,
            Turturro,S., Larsen,P.E., Kovacs,B., Witmer,P.D., Valle,D.,
            Zack,D.J., Nicholson,D.A. and Xu,S.
  TITLE     Inactivation of the microRNA-183/96/182 cluster results in
            syndromic retinal degeneration
  JOURNAL   Proc Natl Acad Sci U S A 110 (6), E507-E516 (2013)
   PUBMED   23341629
REFERENCE   6  (bases 1 to 4676)
  AUTHORS   Templin,J.S., Bang,S.J., Soiza-Reilly,M., Berde,C.B. and
            Commons,K.G.
  TITLE     Patterned expression of ion channel genes in mouse dorsal raphe
            nucleus determined with the Allen Mouse Brain Atlas
  JOURNAL   Brain Res 1457, 1-12 (2012)
   PUBMED   22534482
  REMARK    GeneRIF: This study demonistrated that Ttyh3 gene expression in
            mouse dorsal raphe nucleus
REFERENCE   7  (bases 1 to 4676)
  AUTHORS   Braun,J., Mundhenk,L., Range,F. and Gruber,A.D.
  TITLE     Quantitative expression analyses of candidates for alternative
            anion conductance in cystic fibrosis mouse models
  JOURNAL   J Cyst Fibros 9 (5), 351-364 (2010)
   PUBMED   20624691
  REMARK    GeneRIF: Down-regulation of mTTYH3 may point towards its
            involvement in complex cystic fibrosis pathology. However, markedly
            reduced expression argues against direct compensatory action as
            alternative anion conductance.
REFERENCE   8  (bases 1 to 4676)
  AUTHORS   He,Y., Hryciw,D.H., Carroll,M.L., Myers,S.A., Whitbread,A.K.,
            Kumar,S., Poronnik,P. and Hooper,J.D.
  TITLE     The ubiquitin-protein ligase Nedd4-2 differentially interacts with
            and regulates members of the Tweety family of chloride ion channels
  JOURNAL   J Biol Chem 283 (35), 24000-24010 (2008)
   PUBMED   18577513
REFERENCE   9  (bases 1 to 4676)
  AUTHORS   Suzuki,M. and Mizuno,A.
  TITLE     A novel human Cl(-) channel family related to Drosophila flightless
            locus
  JOURNAL   J Biol Chem 279 (21), 22461-22468 (2004)
   PUBMED   15010458
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC062917.1 and AI843734.1.
            
            On Apr 21, 2015 this sequence version replaced NM_175274.4.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062917.1, AK052340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4658              BC062917.1         1-4658
            4659-4676           AI843734.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 79.16 cM"
     gene            1..4676
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /note="tweety family member 3"
                     /db_xref="GeneID:78339"
                     /db_xref="MGI:MGI:1925589"
     exon            1..327
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1779
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein tweety homolog 3; volume-regulated anion channel
                     subunit Ttyh3"
                     /codon_start=1
                     /product="protein tweety homolog 3 isoform 2"
                     /protein_id="NP_780483.2"
                     /db_xref="CCDS:CCDS19822.1"
                     /db_xref="GeneID:78339"
                     /db_xref="MGI:MGI:1925589"
                     /translation="MAGVSYAAPWWVSLLHRLPHFDLRWEATSSQFRPEDADYQQALL
                     LLGATALACLALDLLFLLFYSFWLCCRRRKTDEHLDADCCCTAWCVIITTLVCSAGIA
                     VGFYGNGETSDGIHRATYSLRHANRTVAGVQDRVWDTAAALNRTAEPNLQSLERQLAG
                     RQEPLRAVQRLQTLLGTLLGYTAAIPFWRNPGVSLEVLAEQVDLYDWYRWLGYLGLLL
                     LDVIICLLVLVGLIRSSKGILVGVCLLGVLALVISWGALGLELAVSVGSSDFCVDPDT
                     FVTKMVEEHSVLSGDILQYYLACSPRATNPFQQKLSGSHKALVEMQDVVAELLRNVPR
                     EHPATKDPLLRVQEVLNGTEVNLQHLTALVDCRSLHLDYVQALTGFCYDGVEGLIYLA
                     LFSFVTALMFSSIVCSIPHTWQQKRGPDDDGEEETAPGPRQAHDSLYRVHMPSLYSCG
                     SSYGSEASIPAAAHTVSNAPVTEYMSQNANFQNPRCENTPLIGRESPPPSYTSSMRAK
                     YLATSQPRPDSSGSGH"
     exon            328..497
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            498..609
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            610..830
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            831..926
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            927..999
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1075
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1131
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1224
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1317
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1454
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1628
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1704
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..4659
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4638..4643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /note="hexamer: AATAAA"
     polyA_site      4658
                     /gene="Ttyh3"
                     /gene_synonym="2900029G13Rik; mTTY3; TTY3"
                     /note="major polyA site"
ORIGIN      
        1 ggtgaacaaa gaggcggcgg gcgcgggcgg ccgagcggag ccgagcgcag ccgagccggg
       61 ccgagccggg ccgagccggg ccgggccagg gcccctggaa gcgggcacga tgagggcagc
      121 tgagcagggg tcggcgggtc cctgagcccc gtgcatccgg cagcgaggga agcccggcgc
      181 agcgcccgtg gccgcccgcc cgccatggcc ggggtcagct acgcggcgcc ctggtgggtg
      241 agcctcctgc accggctgcc gcacttcgac ctgcggtggg aggccaccag cagccaattc
      301 cggcccgagg acgccgacta ccagcaggcg ttgctgctgc tgggggccac cgccctggcc
      361 tgcctggccc tagatctcct cttcctactc ttctattcct tctggctgtg ctgccggagg
      421 agaaagacag acgagcacct tgatgcagac tgctgctgca ctgcctggtg cgtcatcatc
      481 accacactgg tctgcagcgc gggcattgca gtgggattct acggcaacgg cgagaccagc
      541 gatggcattc atcgagccac ctactcacta cgccatgcca accgcacagt ggcaggggtc
      601 caggaccgcg tgtgggacac ggctgccgcc ctgaaccgca cagcggagcc caacctgcag
      661 agcctggaga ggcagctggc tgggcggcag gagccactga gggctgtgca gcggctgcag
      721 accctgctgg ggaccctgct gggctacacc gctgccatcc ccttttggag gaaccccgga
      781 gtatcgctgg aggtgctagc tgaacaggtg gacctctatg actggtacag gtggctgggg
      841 tacctgggcc tgctcttgct tgatgttatc atctgcctcc tggtgctggt gggcctcatc
      901 cgaagctcca agggtatctt ggttggggtc tgcttgctgg gtgtcttggc cctggtcatc
      961 agctggggtg cgctcggctt ggagctggct gtgtctgtgg gctccagcga cttctgcgta
     1021 gaccctgaca cctttgtgac caagatggtg gaggagcact cagtactgag tggggacatt
     1081 ttgcaatact acttggcttg ctcaccccgt gccaccaacc ccttccagca gaagctgtcg
     1141 ggcagccaca aggctctggt ggaaatgcaa gatgttgtcg cggagctgct gaggaacgtc
     1201 ccccgggagc accccgccac caaggatccc ttgctccgag tccaggaggt gctaaatgga
     1261 acggaagtga atctccagca cctcaccgcc ctggtggact gccgcagctt gcacctggac
     1321 tacgtgcagg ctctgacagg cttctgctat gatggtgtgg agggcctcat ctacctggcc
     1381 ctcttctcct ttgtcacagc cctcatgttc agctccatcg tctgcagcat cccacacacc
     1441 tggcagcaga agagaggtcc agatgacgat ggggaggaag agactgcgcc agggccgcgg
     1501 caggcacatg acagccttta ccgagtgcac atgcccagtt tgtacagctg cggaagcagc
     1561 tatggcagcg aagccagcat cccagccgct gcccacaccg tcagcaatgc cccagtcacc
     1621 gagtacatga gccagaatgc caatttccag aacccccgct gtgagaacac ccccctcatt
     1681 gggcgcgagt ccccaccgcc ctcatacacc tccagcatga gagccaaata cctcgccacg
     1741 agccagcctc gccccgactc cagcggcagc gggcactaga ccgcacaccg gccagccatc
     1801 tgcccccacg tgccaatgcc ccacccttcc tatgccagca ccgctgcttc cgccctgctg
     1861 gaccttccac aagccacacc gggccagccc taggcacacc tgaaggcccc cccgctatac
     1921 ccaccctgca ggaggcatgg aggtgcccag catgagctcc gggccagcct gtgaagtgag
     1981 gcttctgggg tgctcacctg gacagtcgct gccaccagca acctgaccct gcctgcctcc
     2041 gtctcccctg cccttctgtc ctcttgggct ccctgagccc tgctctgtgc cgggtgcttc
     2101 caccctgcct gcgttccttc tacaggtgac ccttcatgct gccccttgca ctctgggcgt
     2161 ccccctcccc acagctacag cgagcctttc ctggtactgc cctaccgcca cactcctgag
     2221 aatcccatct ccattcacag gcaggggacc ctgtgcctgg cgcccacctg ctcatcaccg
     2281 aacggcccct tcatactctc tggtctttgc aacgtccggc gcctgccttc ccttagccac
     2341 ctggcttact tggctcttcc tgttggttct gagctgcctc tagggtgggg ctcttcttac
     2401 acctcctccc cacatgactc ttccctcagt gcccagccag ccctccccag acagcaggtg
     2461 ccaagctgag cccaggtcac tccctggcct ctcagtgctg gccaccttct tggtgccaaa
     2521 ccctctttcc cattcattcc tgaagagtgg cagctttgtc ttcccctttg tttttgcact
     2581 gaccacactt gtcatctcca gggcagtctg ggacataggc gacgcaggac actgcccact
     2641 ttcctctcgg tttcattctc acccaggaca agcggcagtg ccctctggag cccagccttg
     2701 ggggctggac ctgtgacggc tgtgaatgtg cccccagcct cagaaggccc cccccacccc
     2761 ccgctgggac taaccactaa cctcactctc agctgccatg ggcactcctg gctgaccctg
     2821 agccagcaag cgtgacccta agtctagcct ggagccaggg ctagagtggt catttctttg
     2881 tggggtgctg ccagggaggg gccagaccca caggctactc aaagggccta gagacccctc
     2941 cccaggcagg tgctgcccca ggaggagcat gtcctggggt ccggggactg aagtccatgt
     3001 ggcctcagcc ccccacaccc agaacaccgc ttgcctaagg tgcttttggc tttagtgtgt
     3061 gatgtttgct gtgcttctgg gctgaattag cttccaaatc aggacctgga gcctctaccc
     3121 tggcccagcc agccagtgtg agctctggtc tgtgagatgg gcagctacgg gccagtggag
     3181 cagcatgtgg tgggaggggc aaggctggga cccagtggtt tacagacctg tggccctcct
     3241 ggagcaacct ggcagctacg gatcccagaa ccccctgggc ttcagctccc ccagagggga
     3301 gaggctccac gttgctttcc ttccccaaaa tccctttctt tgtgctggtg tctgggacca
     3361 aaaggagtgg gcagaggact cggagggcct aggggtccca gtcggggcat ctgtagctcc
     3421 taagcacgac aacatcagtg caggggaccc tggccttgac tccaactggc ctggcgccag
     3481 gaacctccag ggccagagca gcccagctgc agccagcctg cccactatgg gtatgttcct
     3541 ggcctaaggt ccggagggag gtttggggta tccctgcctg ggtgcctggg tgtgccctgg
     3601 ggcctctcag aagcacaaat gctgccccct ggccgtgagc aggccacaag gtgaatgtat
     3661 atagcatgag aggcgggcac tgcccggacg tggctgtgaa cttgtgctgt ctcgggagtc
     3721 ctgaccttct gtgcgtgagt gcccccatct gtgacgtttc actcaccgag gctgaagaaa
     3781 ggaagcaggg gaaatgaaag caggggtttc tcgccctgac ccctgcggag gagacggctc
     3841 ctaccactgc ggttggcttc atttcgtttt cctgatttct ggggtgccac ttacctactc
     3901 aatcccagtg gtccaccccc acatccccag ggagtgagca gtccagtgcc agctgcctgt
     3961 gattggtccc cagtccctat tacccaaggg gaccctacag ctctggtggg taacaaggag
     4021 ggctaagcca ccaaaccaga gcccgatccc ttgccgagcc aggaggaggg atctggctga
     4081 gaaaactgat aggactggag gcccccaccc caaccaacac tctctggttt atgtgagtag
     4141 cagaagatcc cggcctggag catccttcaa gcccttctcc ctgtgcccac cccgcccccc
     4201 ccccccccca tatcactatg caattcttga ccccagctcc aaagcttgcc ctacccggtc
     4261 ccagctctgt ccggcccaga aggtggctag ctggtgggcc acaggtgacc agggtctctt
     4321 tgtttttcat cacagcggtg gtgtgccgca cccttcctcc catatgtgat tttgtgagat
     4381 tgcctcccag ttacggtccc tctgcctgca tctgccccca gtggactatg tcatctgaat
     4441 cgagccagcc ccaagttccc ctccagcctc tgtagggcca tggctgtgtg ttactgttgc
     4501 tgtgctttca ttttttaaac tgggtttggg gtttgatttt tatttctgtg gggaacttta
     4561 tttttcttgg caaataacta aagttcttgt ccatgtaatt tctgtggtct ctattcagct
     4621 tgggtttcat gttttaaaat aaacaatttt aagaaacaga aaaaaaaaaa aaaaaa
//
