LOCUS       NM_021324               3057 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus tweety family member 1 (Ttyh1), transcript variant 2,
            mRNA.
ACCESSION   NM_021324
VERSION     NM_021324.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Qu,F., Li,W., Xu,J., Zhang,R., Ke,J., Ren,X., Meng,X., Qin,L.,
            Zhang,J., Lu,F., Zhou,X., Luo,X., Zhang,Z., Wang,M., Wu,G., Pei,D.,
            Chen,J., Cui,G., Suo,S. and Peng,G.
  TITLE     Three-dimensional molecular architecture of mouse organogenesis
  JOURNAL   Nat Commun 14 (1), 4599 (2023)
   PUBMED   37524711
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Su-Feher,L., Rubin,A.N., Silberberg,S.N., Catta-Preta,R., Lim,K.J.,
            Ypsilanti,A.R., Zdilar,I., McGinnis,C.S., McKinsey,G.L.,
            Rubino,T.E. Jr., Hawrylycz,M.J., Thompson,C., Gartner,Z.J.,
            Puelles,L., Zeng,H., Rubenstein,J.L.R. and Nord,A.S.
  TITLE     Single cell enhancer activity distinguishes GABAergic and
            cholinergic lineages in embryonic mouse basal ganglia
  JOURNAL   Proc Natl Acad Sci U S A 119 (15), e2108760119 (2022)
   PUBMED   35377797
REFERENCE   3  (bases 1 to 3057)
  AUTHORS   Cao,Y., Wu,H.N., Cao,X.L., Yue,K.Y., Han,W.J., Cao,Z.P.,
            Zhang,Y.F., Gao,X.Y., Luo,C., Jiang,X.F., Han,H. and Zheng,M.H.
  TITLE     Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem
            Cells Through Ca2+/NFATc3 Signaling
  JOURNAL   Front Cell Dev Biol 9, 779373 (2021)
   PUBMED   34869383
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3057)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3057)
  AUTHORS   Kumada,T., Yamanaka,Y., Kitano,A., Shibata,M., Awaya,T., Kato,T.,
            Okawa,K., Abe,T., Oshima,N., Nakahata,T. and Heike,T.
  TITLE     Ttyh1, a Ca(2+)-binding protein localized to the endoplasmic
            reticulum, is required for early embryonic development
  JOURNAL   Dev Dyn 239 (8), 2233-2245 (2010)
   PUBMED   20568244
  REMARK    GeneRIF: Ttyh1 plays an indispensable role during mitosis in early
            embryogenesis, possibly by maintaining Ca(2+) homeostasis in the
            ER.
REFERENCE   6  (bases 1 to 3057)
  AUTHORS   Matthews,C.A., Shaw,J.E., Hooper,J.A., Young,I.G., Crouch,M.F. and
            Campbell,H.D.
  TITLE     Expression and evolution of the mammalian brain gene Ttyh1
  JOURNAL   J Neurochem 100 (3), 693-707 (2007)
   PUBMED   17116230
REFERENCE   7  (bases 1 to 3057)
  AUTHORS   Sansom,S.N., Hebert,J.M., Thammongkol,U., Smith,J., Nisbet,G.,
            Surani,M.A., McConnell,S.K. and Livesey,F.J.
  TITLE     Genomic characterisation of a Fgf-regulated gradient-based
            neocortical protomap
  JOURNAL   Development 132 (17), 3947-3961 (2005)
   PUBMED   16079153
REFERENCE   8  (bases 1 to 3057)
  AUTHORS   Abramova,N., Charniga,C., Goderie,S.K. and Temple,S.
  TITLE     Stage-specific changes in gene expression in acutely isolated mouse
            CNS progenitor cells
  JOURNAL   Dev Biol 283 (2), 269-281 (2005)
   PUBMED   15890332
REFERENCE   9  (bases 1 to 3057)
  AUTHORS   Suzuki,M. and Mizuno,A.
  TITLE     A novel human Cl(-) channel family related to Drosophila flightless
            locus
  JOURNAL   J Biol Chem 279 (21), 22461-22468 (2004)
   PUBMED   15010458
REFERENCE   10 (bases 1 to 3057)
  AUTHORS   Campbell,H.D., Kamei,M., Claudianos,C., Woollatt,E.,
            Sutherland,G.R., Suzuki,Y., Hida,M., Sugano,S. and Young,I.G.
  TITLE     Human and mouse homologues of the Drosophila melanogaster tweety
            (tty) gene: a novel gene family encoding predicted transmembrane
            proteins
  JOURNAL   Genomics 68 (1), 89-92 (2000)
   PUBMED   10950931
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK043998.1, AK018148.1 and
            AK081274.1.
            
            On Apr 4, 2015 this sequence version replaced NM_021324.5.
            
            Summary: This gene encodes a member of the Tweety family of
            membrane proteins. Members of this family contain five predicted
            transmembrane regions that are arranged in a characteristic
            pattern. In mouse, the protein is predominantly localized to the
            endoplasmic reticulum and displays calcium binding activity.
            Targeted knock out of this gene results in early embryonic
            lethality prior to the blastocyst stage. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Apr
            2015].
            
            Transcript Variant: This variant (2) uses an alternate exon in the
            3' coding region that causes a frameshift compared to variant 1. It
            encodes isoform 2, which has a shorter and novel C-terminus
            compared to isoform 1. Variants 2 and 3 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK018148.1, AK043998.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AK043998.1         2-57
            57-2993             AK018148.1         2-2938
            2994-3057           AK081274.1         1240-1303
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 2.38 cM"
     gene            1..3057
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="tweety family member 1"
                     /db_xref="GeneID:57776"
                     /db_xref="MGI:MGI:1889007"
     exon            1..251
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1478
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein tweety homolog 1; volume-regulated anion channel
                     subunit Ttyh1"
                     /codon_start=1
                     /product="protein tweety homolog 1 isoform 2"
                     /protein_id="NP_067299.2"
                     /db_xref="CCDS:CCDS20730.1"
                     /db_xref="GeneID:57776"
                     /db_xref="MGI:MGI:1889007"
                     /translation="MGAPPGYRPSAWVHLLHQLPRADFQLRPVPSGFAPRDQEYQQAL
                     LLVAALAGLGLGLSLIFIAVYLIRFCCCRPPEPHGAKSPPPGGGCVTWSCIAALLVGC
                     AGIGIGFYGNSETSDGVSQLSSALLHANHTLSTIDDVVLETVERLGEAVKTELTTLEE
                     VLSVRMELVAATRGARRQAEAAAQYLQGLAFWQGVSLSPVQVAEDVTFVEEYRWLAYV
                     LLLLLVLLVCLFTLLGLAKQSKWLVVVMTAMSLLVLVLSWGSMGLEAATAVGLSDFCS
                     NPDTYVLNLTQEETGLSSDILSYYFLCNQAVSNPFQQRLTLSQRALASIHSQLQGLER
                     EAIPQFSAAQKPLLSLEETLNVTERSFHQLVALLHCRSLHKDYGSALRGLCEDALEGL
                     LFLMLFSLLSAGALATTLCSLPRAWALFPPSDDYDDTDDDDPFNPQESKRFVQWQSSI
                     "
     exon            252..430
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            431..542
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..763
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..859
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..932
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1008
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1064
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1157
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1250
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1393
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1439
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1515
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..3057
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2955..2960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="hexamer: AATAAT"
     regulatory      2965..2970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="hexamer: AACAAA"
     regulatory      2970..2975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="hexamer: AACAAA"
     polyA_site      2971
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="major polyA site"
     regulatory      2974..2979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="hexamer: AATAAA"
     polyA_site      2983
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
                     /note="major polyA site"
     polyA_site      2989
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
     polyA_site      2997
                     /gene="Ttyh1"
                     /gene_synonym="4930459B04Rik; 6330408P11Rik; mTTY1; tty;
                     TTY1"
ORIGIN      
        1 cccaatctcc acccccggcc ctccgggctc ctgctcccgg gagccccgca aaccccgctc
       61 cgcccgcgcc ccgcacagca ccgaggctcc cggcccggtc cgccccgctg cccctccccg
      121 gggccatggg ggcacccccg ggctaccgac cctctgcctg ggtgcatctc ctccaccagc
      181 tgccccgcgc cgacttccag ctccgcccgg tgcccagcgg ctttgctccc cgcgaccaag
      241 agtaccagca ggccctgctg ctggtggcag ccttggctgg gcttggttta ggcctgagcc
      301 tcatcttcat cgctgtctac ctcatccgct tctgctgctg caggcctcca gaaccccatg
      361 gggccaagag tcccccaccc ggaggaggtt gcgtcacctg gagctgcatt gctgcccttc
      421 tcgtgggctg tgccggcatt ggcattggtt tctatggtaa cagcgagacc agcgatgggg
      481 tgtcccagct cagctcagca ctgctgcacg ccaaccacac gctcagcacc atcgatgacg
      541 tggtgctgga aacggtggag aggctgggtg aggcagtgaa gacggagctg accaccctgg
      601 aggaagtgct gtcagtccgc atggagctgg tggctgccac caggggagcc cgaaggcagg
      661 ctgaagctgc tgctcagtac ctgcaaggac tggccttctg gcaaggagtg tccctgagcc
      721 ccgtacaggt ggctgaagat gtgactttcg tggaggagta caggtggctg gcctatgtcc
      781 tcctgctgct cttggtgctt ctggtttgtc tcttcaccct cctgggcctg gcaaagcaga
      841 gcaaatggct ggtggttgtg atgactgcca tgagtctcct ggtgcttgtc ctgagctggg
      901 gttctatggg cctagaggct gccacagctg tgggcctcag tgacttctgc tccaacccag
      961 acacctatgt gctgaacctg acccaggagg agacagggct cagctcagac atcctcagct
     1021 attatttcct gtgcaaccag gcggtctcca accccttcca acagaggctg acgctgtccc
     1081 agcgtgctct agccagcatc cactctcagc tgcagggcct ggagcgggaa gctatccctc
     1141 aattttctgc tgcacagaag cccttgttat ccttggagga gacactgaat gtgacagaaa
     1201 gaagcttcca ccagttggtg gcgctgctgc actgccgcag tctgcacaag gactatggct
     1261 cagcactccg aggcctgtgt gaagatgccc tagagggcct actgttcctg atgctctttt
     1321 ctctcctgtc tgcgggggcc ctagccacca ctctgtgcag cctgccccgc gcctgggccc
     1381 tcttcccacc cagtgatgac tacgatgaca cagatgacga tgaccctttc aaccctcagg
     1441 aatccaaacg ctttgtgcag tggcagtctt ccatctgagc ctcccctcca gtctggagcc
     1501 tgcctctcct ctatgctcct ttcctggctg ctggagaagg ccccactaac ctgaccttga
     1561 ctgggctctg accactaaca ctgctggcta tggacatctc acttaggatg gcaacctgct
     1621 ctggccctaa cacccacccc cccatcccca tttctcctta gcacagggga ttgctgaggt
     1681 ggcacaacag ggaccagggg tggctgggaa gggggctgac ctatacagcc tggtgttcct
     1741 cacctctggc tgccagtcct gtccttggag ggatgaagct ggaggcaggt acaggcacgt
     1801 ccctgtttcc tgccataccc cagcgatctc aacctatggc actaacttgg aagagggctg
     1861 atttgaaata aaagggaagg ctttatttta caagcagcta ggtccttctt atccacccca
     1921 actactgaga gaaggaagtc ctgactgggc tagggacact cctcctctgg tggttgaaag
     1981 gcaactctcc tcttgtccag gctcagcact gcattgcttc tataagtaac atgtgaatcc
     2041 tgacaagggg gaagaagcag ataccggaag agaataagcc tagcactaaa tctaaacagg
     2101 cgtgcagcca caggaacagt caagcttcca gtcccgtgga atgtacaaag cctgctgggt
     2161 ttaggaaagg cgtggaatca acttgcttcc tagcagctca cacccagcac aagactccag
     2221 aaaaccaacg cttgtatcag aatagggaca gaagtcttcg aagagaaagt agaatatttg
     2281 cttgtagtgc caccagggtg atatgaaggg ggaaggttta aggaagatat tataaatcca
     2341 ggcaggaaaa gatgaggcac tcaataagtt atcaagtaaa aacaaacatg gcagagtcaa
     2401 aagggaaatt tattgtctgg ggaacaatat gtatgtagtt ctcatttgtt taaaaaacaa
     2461 atagcataca tggcaaagaa aaacgtggtt aatacataaa cataattcaa aaagcttcta
     2521 ttatctaaca aaaaagacct caatttaata ataagtaact gagaaaaaca aaaaccccaa
     2581 aacaaatgga taaaattgtc ttttttgtct ttgttgttgt tgttttggag acagggtttc
     2641 tctgtgaaac agccctgggc tgtcctggaa ctctctttgt agaccaggct ggccttgaac
     2701 tcacagagaa gagcctgcct cagcctctgc ctccctagtg aggagattaa cggcgtgtga
     2761 cggcgtgtat caccacaccc aactaaagtg ttcaaattct aatgattagc aaacacaatg
     2821 tgctactttc ccctttcgag tacctatttg ttgtgggatt tagagtggag tgtgggctgg
     2881 tgctggtctg gaggcccagg gaacaggttc tctaaatatg ctgcccagag actgtgaatc
     2941 accagctatg tgaaaataat aataaacaaa acaaataaac aaacataaat atgcttacat
     3001 gtgagaggat aaaaactgca tctcttatcc tggtaatcct ttgagggacc agcttcc
//
