LOCUS       NM_001083618            2939 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tubulin tyrosine ligase-like family, member 9 (Ttll9),
            transcript variant 1, mRNA.
ACCESSION   NM_001083618
VERSION     NM_001083618.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ye,B., Liu,B., Hao,L., Zhu,X., Yang,L., Wang,S., Xia,P., Du,Y.,
            Meng,S., Huang,G., Qin,X., Wang,Y., Yan,X., Li,C., Hao,J., Zhu,P.,
            He,L., Tian,Y. and Fan,Z.
  TITLE     Klf4 glutamylation is required for cell reprogramming and early
            embryonic development in mice
  JOURNAL   Nat Commun 9 (1), 1261 (2018)
   PUBMED   29593216
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Konno,A., Ikegami,K., Konishi,Y., Yang,H.J., Abe,M., Yamazaki,M.,
            Sakimura,K., Yao,I., Shiba,K., Inaba,K. and Setou,M.
  TITLE     Ttll9-/- mice sperm flagella show shortening of doublet 7,
            reduction of doublet 5 polyglutamylation and a stall in beating
  JOURNAL   J Cell Sci 129 (14), 2757-2766 (2016)
   PUBMED   27257088
  REMARK    GeneRIF: Ttll9 plays essential roles in maintaining the normal
            structure and beating pattern of sperm flagella by establishing
            normal heterogeneous polyglutamylation patterns.
REFERENCE   3  (bases 1 to 2939)
  AUTHORS   van Dijk,J., Rogowski,K., Miro,J., Lacroix,B., Edde,B. and Janke,C.
  TITLE     A targeted multienzyme mechanism for selective microtubule
            polyglutamylation
  JOURNAL   Mol Cell 26 (3), 437-448 (2007)
   PUBMED   17499049
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK006027.1, AK015388.1 and AK041200.1.
            
            Transcript Variant: This variant (1) differs in the 3' UTR and
            coding sequence compared to variant 2. The resulting isoform (1)
            has a shorter and distinct C-terminus compared to isoform 2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006027.1, AK015388.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-384               AK006027.1         1-384
            385-921             AK015388.1         365-901
            922-922             AK006027.1         918-918
            923-1797            AK015388.1         902-1776
            1798-2939           AK041200.1         624-1765
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.41 cM"
     gene            1..2939
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /note="tubulin tyrosine ligase-like family, member 9"
                     /db_xref="GeneID:74711"
                     /db_xref="MGI:MGI:1913589"
     exon            1..71
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..1442
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tubulin tyrosine ligase like family 9; probable tubulin
                     polyglutamylase TTLL9; putative polyglutamylase;
                     tubulin--tyrosine ligase-like protein 9"
                     /codon_start=1
                     /product="probable tubulin polyglutamylase TTLL9 isoform
                     1"
                     /protein_id="NP_001077087.1"
                     /db_xref="CCDS:CCDS38283.1"
                     /db_xref="GeneID:74711"
                     /db_xref="MGI:MGI:1913589"
                     /translation="MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRP
                     GWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRF
                     RKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIF
                     LFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQ
                     RYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLT
                     NVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKV
                     IISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDM
                     EARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGCVNDRKEQLRQLF
                     RSLQAQRKAPS"
     exon            72..115
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            116..208
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            209..320
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            321..506
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            507..575
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            576..650
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            651..741
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            742..782
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            783..870
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            871..929
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1126
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1240
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1365
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..2939
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1747..1752
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1769
                     /gene="Ttll9"
                     /gene_synonym="1700016F23Rik; 4930509O20Rik"
                     /note="major polyA site"
ORIGIN      
        1 aacgagacgc acatgctttc tagggtgccc gcggcggttt ggtttgggcc caactcatgt
       61 cgcgacagaa gaatcaaaat tccaagggcc atggagtgtc gaaaggaaaa gagagagagc
      121 agagaacctt aatccgcttc aagaccaccc tcatgaacac actcatggat gttctgcgcc
      181 acaggcccgg atgggtggaa gtcaaagatg aaggcgaatg ggatttctat tggtgtgatg
      241 tcagctggct gcgggagaac tttgaccaca cctacatgga tgagcacgtg cgcataagcc
      301 acttccggaa ccactacgag cttaccagga aaaactacat ggtaaagaac ctgaagcgct
      361 tccggaagta cctggagcgt gagtcaggaa agacggaggc agccaagtgt gacttcttcc
      421 ccaaaacctt tgagatgccc tgtgagtacc atctgtttgt ggaggagttt cgcaagaacc
      481 ccgggatcac ctggatcatg aagcctgtcg cccgatctca ggggaagggc atatttctct
      541 tccggagact gaaagacatc atggactgga ggaagggcac ttcggggaag aaacccaccg
      601 gtgtggagac gcagcctgct cgagccaaca tgaacccctc aggcagccat gacacaagga
      661 gttctgatga ccagaaagat gacctcccgg tggagaacta cgtggctcag cgttacgtgg
      721 aaaaccccta cttaatagga ggccgcaagt ttgacctgcg cgtctacgtg ctggtgatgt
      781 cgtacatccc tctgcgggca tggctgtacc gtgatggctt tgccaggttc tccaacaccc
      841 gattcacact gaatagcatc gatgaccact atgttcacct caccaatgtt gctgtgcaga
      901 agacatcgcc tgactaccac ctaaaaaagg gttgcaagtg gatgcttcag cgcttcaggc
      961 agtacctggc ttccaagcat ggtcctaagg ccgtggagac actcttcagt gacatggaca
     1021 atatcttcat caagagcctg cagagcgtgc agaaggtgat catcagtgat aagcactgct
     1081 ttgagctcta tggctatgac atcctcattg accaggacct caagccgtgg ctcctagaag
     1141 tcaatgcctc tccttccctg acggccagca gccaggagga ctatgaactc aagacctgtc
     1201 tgctggagga taccctgcac gtggtggaca tggaggccag gctcacagga aaggagaaga
     1261 gagtcggggg cttcgatctc atgtggaatg atggccctgt cagccgagag gatgggcctt
     1321 ctgacctatc agggatgggg aactttgtga ccaacactca cctaggctgc gtcaatgacc
     1381 ggaaggagca gctcaggcag ctgttccgat cccttcaagc gcagaggaaa gcccctagct
     1441 gacccccagt cagaagaaat gaggccctgg caggtgccgc cctggcctct ggcagcgcga
     1501 ttccagcaca gcgcaccttt ttccagtctt cacctttttc cagcgtggca gctgtttcac
     1561 tggacaggac cagcttgtac ttggaccaga ctccctggac tccccactcc agccctgtgc
     1621 atctttgaac caggaacagc caatctttgg aactgggtgg gcttatcctt gactgattgg
     1681 caactgggcc caaagaggcc aagcagcaag ttccactgct cagagagcac gtctaatacc
     1741 acatctaata aagcacaggc ttagagctac tcggcactgg gcgtagcttg ggatgctgct
     1801 gacgttttga tgtctcttta atcatgggtc cggtgtgctg agaaactcgt ccacaggagg
     1861 tctgggatag tgccaagccc caaatgtatg gtgtcctctg ctatgtgaaa accagagcta
     1921 gggcctcctg tgtctcagct ggaaatgaga actgggtagt cagggctgct tggccagggt
     1981 ccccgggaaa acccacctcc catttggggc ttttgagttt aaaaccagaa agcaaggctc
     2041 cagttgacag gtagcctggg ctggaaggca acttacccca cggagaaggg gcctccaggg
     2101 ggcccaagca gctctcccta ccttctgggt catctttgtc cataaatgca aacctggcta
     2161 cctaaagtaa gcccgaagga acgtctggga atcaccagca aatcaagaag tgaactgacc
     2221 atgggaggct ggtagcccaa acccacaacc cccaagtatt gacttgaggt tcttgcttcc
     2281 agaatacaga accgatgtgc tctttggctt agcaccctgg caggcaggag aaattccagt
     2341 ggagaaataa tgtgccccac aaacccacca ggtagctgcc atcaaagtga attctgttgg
     2401 gtctgcatct tattcacatg gtttgggagt tcagagctta tgaaaagagt tgggtagaca
     2461 gtatcatctc cccttttacc ctgcaatttg tccttcaggg accacacagc ctctgcctac
     2521 agtcaggagg tcacacattg ctcagccctt gctcaaggtt caagtacctc cttcatcctg
     2581 agctaagctg ccgccttcct ctgaagctgt ggtcttctaa ctagagccca gtgcagtggc
     2641 ctggtcatac catgggaatg cctgtaaaat ccaaggaaac aaatcttcag gcacggacct
     2701 tatttgcctt acatgctttt cttcatcaac acattttaaa atattccttg aaggcatcag
     2761 tattattaaa aaatatttct aatttctagt ttctttccta ttttttgaca gggtccaccc
     2821 tggaacttcc tctgtagacc aggctggcct taaacacatg caatgtgtat tacagttgga
     2881 aatagaaatt tccacctacc tctgcctccc atgtgctggg attaaaagtg tataccacc
//
