LOCUS       NM_172818               2976 bp    mRNA    linear   ROD 06-FEB-2024
DEFINITION  Mus musculus tubulin tyrosine ligase-like family, member 8 (Ttll8),
            transcript variant 1, mRNA.
ACCESSION   NM_172818
VERSION     NM_172818.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ho,K.H., Candat,A., Scarpetta,V., Faucourt,M., Weill,S., Salio,C.,
            D'Este,E., Meschkat,M., Wurm,C.A., Kneussel,M., Janke,C.,
            Magiera,M.M., Genovesio,A., Meunier,A., Sassoe-Pognetto,M.,
            Brill,M.S., Spassky,N. and Patrizi,A.
  TITLE     Choroid plexuses carry nodal-like cilia that undergo axoneme
            regression from early adult stage
  JOURNAL   Dev Cell 58 (23), 2641-2651 (2023)
   PUBMED   37890489
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Gadadhar,S., Alvarez Viar,G., Hansen,J.N., Gong,A., Kostarev,A.,
            Ialy-Radio,C., Leboucher,S., Whitfield,M., Ziyyat,A., Toure,A.,
            Alvarez,L., Pigino,G. and Janke,C.
  TITLE     Tubulin glycylation controls axonemal dynein activity, flagellar
            beat, and male fertility
  JOURNAL   Science 371 (6525) (2021)
   PUBMED   33414192
  REMARK    GeneRIF: Tubulin glycylation controls axonemal dynein activity,
            flagellar beat, and male fertility.
REFERENCE   3  (bases 1 to 2976)
  AUTHORS   Garnham,C.P., Yu,I., Li,Y. and Roll-Mecak,A.
  TITLE     Crystal structure of tubulin tyrosine ligase-like 3 reveals
            essential architectural elements unique to tubulin monoglycylases
  JOURNAL   Proc Natl Acad Sci U S A 114 (25), 6545-6550 (2017)
   PUBMED   28576883
REFERENCE   4  (bases 1 to 2976)
  AUTHORS   Rocha,C., Papon,L., Cacheux,W., Marques Sousa,P., Lascano,V.,
            Tort,O., Giordano,T., Vacher,S., Lemmers,B., Mariani,P.,
            Meseure,D., Medema,J.P., Bieche,I., Hahne,M. and Janke,C.
  TITLE     Tubulin glycylases are required for primary cilia, control of cell
            proliferation and tumor development in colon
  JOURNAL   EMBO J 33 (19), 2247-2260 (2014)
   PUBMED   25180231
  REMARK    Erratum:[EMBO J. 2014 Nov 18;33(22):2735]
REFERENCE   5  (bases 1 to 2976)
  AUTHORS   Harrow,J.L., Steward,C.A., Frankish,A., Gilbert,J.G.,
            Gonzalez,J.M., Loveland,J.E., Mudge,J., Sheppard,D., Thomas,M.,
            Trevanion,S. and Wilming,L.G.
  TITLE     The Vertebrate Genome Annotation browser 10 years on
  JOURNAL   Nucleic Acids Res 42 (Database issue), D771-D779 (2014)
   PUBMED   24316575
REFERENCE   6  (bases 1 to 2976)
  AUTHORS   Bosch Grau,M., Gonzalez Curto,G., Rocha,C., Magiera,M.M., Marques
            Sousa,P., Giordano,T., Spassky,N. and Janke,C.
  TITLE     Tubulin glycylases and glutamylases have distinct functions in
            stabilization and motility of ependymal cilia
  JOURNAL   J Cell Biol 202 (3), 441-451 (2013)
   PUBMED   23897886
  REMARK    GeneRIF: The TTLL8 was required for stability and maintenance of
            ependymal cilia, whereas the polyglutamylase TTLL6 was necessary
            for coordinated beating behavior.
REFERENCE   7  (bases 1 to 2976)
  AUTHORS   Ikegami,K. and Setou,M.
  TITLE     TTLL10 can perform tubulin glycylation when co-expressed with TTLL8
  JOURNAL   FEBS Lett 583 (12), 1957-1963 (2009)
   PUBMED   19427864
  REMARK    GeneRIF: TTLL10 can strongly glycylate tubulin in a TTLL8-dependent
            manner. Furthermore, these two TTLL proteins can glycylate
            unidentified 170-, 110-, 75-, 40-, 35-, and 30-kDa acidic proteins.
REFERENCE   8  (bases 1 to 2976)
  AUTHORS   Rogowski,K., Juge,F., van Dijk,J., Wloga,D., Strub,J.M.,
            Levilliers,N., Thomas,D., Bre,M.H., Van Dorsselaer,A., Gaertig,J.
            and Janke,C.
  TITLE     Evolutionary divergence of enzymatic mechanisms for
            posttranslational polyglycylation
  JOURNAL   Cell 137 (6), 1076-1087 (2009)
   PUBMED   19524510
REFERENCE   9  (bases 1 to 2976)
  AUTHORS   van Dijk,J., Rogowski,K., Miro,J., Lacroix,B., Edde,B. and Janke,C.
  TITLE     A targeted multienzyme mechanism for selective microtubule
            polyglutamylation
  JOURNAL   Mol Cell 26 (3), 437-448 (2007)
   PUBMED   17499049
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029745.1, AK030151.1, BB616904.1 and AM690752.1.
            
            [WARNING] On Mar 25, 2024 this sequence was replaced by
            NM_172818.4.
            
            On Apr 27, 2010 this sequence version replaced NM_172818.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to an upstream in-frame start codon. The uAUG appears to
            be restricted to rat and mouse, and has a weak Kozak signal
            compared to a strong one for the better conserved downstream AUG,
            which raises the possibility of the dAUG being use due to leaky
            scanning. The update changes the protein length from 781 aa to 832
            aa, but the splice sites are unaffected.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030151.1, BC116293.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849381,
                                           SAMN00849384 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AK029745.1         2-10
            10-104              AK030151.1         3-97
            105-343             BB616904.1         81-319
            344-2842            AM690752.1         1-2499
            2843-2976           AK030151.1         2836-2969
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 44.39 cM"
     gene            1..2976
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /note="tubulin tyrosine ligase-like family, member 8"
                     /db_xref="GeneID:239591"
                     /db_xref="MGI:MGI:1922902"
     exon            1..563
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..2842
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein monoglycylase TTLL8; TTL domain protein;
                     tubulin--tyrosine ligase-like protein 8"
                     /codon_start=1
                     /product="protein monoglycylase TTLL8 isoform 1"
                     /protein_id="NP_766406.2"
                     /db_xref="CCDS:CCDS27735.2"
                     /db_xref="GeneID:239591"
                     /db_xref="MGI:MGI:1922902"
                     /translation="MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQK
                     NQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKA
                     IKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALE
                     RFDDIHDVMSRLVKNEIPYLLWTIKRDVVDYHSLTCDQMLNHYGKTASFTTKIGLCLN
                     MRSLPWYVQANPNTFFPRCYGLCTESEKQEFLDDFRRTVAASILKWVVLHQNYCSKVK
                     GKSKKEEAKNSDPSPKKDPENPDLKLPSLSGQVVDTACKVCQAYLGQLEHEDIDVSEA
                     STEALSEEEWNDLTQQYYLLVHGNASITDSKSYFAQCQALLSKISSVNPQTEIDGIRN
                     IWIIKPAAKSRGRDIVCMDRVENILSLVAADSQTTKDNKWVVQKYIETPMLIYDTKFD
                     IRQWFLVTDWNPLTIWFYKESYLRFSTQRFSLDKLDSAIHLCNNSIQRRLKNDKERSP
                     LLPCHNMWTSTRFQEYLQKRGRGGTWGSIIYPSMKRAVTNAMRVAQDHVEARKNSFEL
                     YGADFILGRDFKPWLIEINSSPTMHPSTPVTAQLCAQVQEDTIKVVVDRKLDRNCDIG
                     NFELLWRQPAVELPPFNGSDLCVEGISVKKAKKQMPPIASVGLSESLLDAPPKVRSAR
                     ALMETVIRPPRTTVRQDWKREEAKVLSTTWSMPVMDAEVRGRAKPIYAFEVNDYQHVD
                     NKSHKSGYTRVQSSKVPGVTLTSAQHPALFAQTMKPTQMTSSPPPTASGNHRDSSPFC
                     PIVFEELWLHPNSQRRPSSCILQSRAQGWIRGIP"
     exon            564..655
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            656..779
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            780..850
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            851..979
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1094
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1223
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1410
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1559
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1803
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..2239
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2976
                     /gene="Ttll8"
                     /gene_synonym="1700019P01Rik; 4933401B17"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atccctccac tttcacccca ccacgcctgt tcaaccttct ttccctcacc tccccacttt
       61 cctccaaact tcgtaacggc ctcccctttc tgaccctaag cctgcgcccc aatttgtgtc
      121 ctgtctccct acctcgacct ctccttctta gctccagacc tgtatttctc tccagactgg
      181 atgtccctct tggacttccc taactctggc ccaagtctat ctcttcaacc ccatacttac
      241 ttccttaccc ccgagttgtc cctctaccct aacttcttca tctcatctgt ctcccactga
      301 cctttcttct catctgactt ctcacacctc aagtttccca gtcatgtcgt gtcccccgac
      361 cccaaaccct cctttcaggc ctccctccca cacccgggtc ctccgaaccc cacccttgcc
      421 tccctgggta tgtctgaact ctaaatcttt gtcaacaggg gttgggggcc aaaagaacca
      481 gctacgagag gcgagcatgg agaacggcga gaggaagaag ctgtcttcca ctttgtcaga
      541 tggagatcac aaagaagaaa ataagttaaa acaaggaatt ccccaagatt tgtcttcttc
      601 ccccaaatta gacagataca aaatagcaag acagttaaca gaaaaagcca tcaaggagag
      661 gaagattttc tccatctatg gacactaccc tgtcatccgg gccaccttgc gaagaaaggg
      721 ctgggtggag aagaagttca atttcttccc caaagctctc cagaaccttg gaagtgaaga
      781 taaaagtgct gagaccaaag aaaatcaaga aatagccttg gagagattcg atgatatcca
      841 cgatgtgatg tccaggttgg taaagaacga gatcccatac ctcctctgga ctatcaagag
      901 ggatgttgtg gactaccaca gcctgacctg tgaccagatg ctaaatcact acgggaagac
      961 agcatcattc acaaccaaga ttggactctg cctgaacatg agaagtctgc cttggtacgt
     1021 ccaagccaac ccgaacacct tcttcccacg gtgctatggc ctttgcactg agagcgagaa
     1081 gcaggaattc ttggatgact tccggcgcac ggtggctgcc agcatcctca agtgggtagt
     1141 ccttcaccag aattactgca gcaaagtcaa gggcaagagt aagaaggagg aggccaagaa
     1201 cagtgacccc agccccaaga aagatcctga aaatcctgac ctcaagctac caagcctctc
     1261 gggacaggtt gtagacacag cttgcaaggt gtgccaggcc tacctgggac agctagagca
     1321 tgaagacata gacgtatcgg aggccagcac cgaggccttg tctgaggagg aatggaatga
     1381 cctgacacag cagtactatc tactggttca tggcaatgct tccatcactg attcgaaaag
     1441 ttactttgcg caatgccaag ctctgctgag taagatcagt tctgtgaacc ctcagacaga
     1501 gatcgatggg atccggaaca tctggatcat aaagcctgca gccaagtccc ggggtcgaga
     1561 tattgtgtgt atggaccgtg tggagaacat cctgagtctg gtggccgcag acagccagac
     1621 cacaaaggac aacaaatggg tggtccagaa gtacatcgag accccaatgc tcatctacga
     1681 caccaagttt gacatcagac agtggttcct cgtcacagac tggaatcccc taaccatctg
     1741 gttctacaaa gaaagctacc tccgcttctc cacacagcgc ttctccctgg acaagctgga
     1801 cagcgccatc cacctgtgta acaactccat ccagaggcgc ctcaaaaatg ataaagagcg
     1861 cagtccgctg ctaccttgtc ataacatgtg gaccagcact cgtttccagg agtacctgca
     1921 gaagaggggc cgaggaggaa cgtggggtag catcatctac ccgtctatga aaagagctgt
     1981 caccaatgcc atgagggtag cccaggacca cgtagaagcc cgtaagaaca gcttcgagct
     2041 ctacggagcc gacttcatcc tggggcgaga cttcaagcca tggcttatcg agatcaactc
     2101 cagccccacc atgcacccct ccactcccgt cacagctcag ctctgtgccc aggtgcagga
     2161 ggacaccatc aaggtggtgg tggatcgcaa actggaccga aactgtgaca tcggcaactt
     2221 tgagcttctg tggcggcagc ctgccgtgga gctgccaccg ttcaacgggt ctgacctctg
     2281 tgtggaaggt atcagcgtga agaaagccaa gaagcaaatg ccgcccattg ccagcgtcgg
     2341 cctctcagaa tcactcttag atgctccacc caaagtgcgg agtgcccggg ccctgatgga
     2401 aacagtgatc aggcctccaa gaacgaccgt gcggcaggac tggaaacggg aagaagcaaa
     2461 ggtactttca accacttggt ccatgccagt aatggatgca gaggtcagag gtcgagcaaa
     2521 gcccatctat gcattcgagg tcaatgacta ccaacatgta gacaataagt cccacaagag
     2581 cggttatacc agagtccaat ccagtaaagt cccaggggta accttaacaa gtgcacagca
     2641 ccctgccctg tttgcacaga ctatgaaacc cacacagatg acctcgtctc caccacccac
     2701 tgcgtcaggt aaccatagag actcttcgcc tttctgtccc attgtatttg aggaactctg
     2761 gctccacccc aattctcaga gacgaccttc aagctgtatt ctccaaagca gggcacaggg
     2821 ctggatcagg gggatcccct gagaggatct cacaaggggt gcgggggcaa cactggcact
     2881 gacctagcca ggcactaggg tctgcggtgt aatgttggcc aaggtgagca gcatacttgg
     2941 gtcctattgc aatagtccca gtggggtttc ctcagt
//
