LOCUS       NM_001357217            7333 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tubulin tyrosine ligase-like family, member 7 (Ttll7),
            transcript variant 3, mRNA.
ACCESSION   NM_001357217 XM_006502088
VERSION     NM_001357217.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Ping,Y., Ohata,K., Kikushima,K., Sakamoto,T., Islam,A., Xu,L.,
            Zhang,H., Chen,B., Yan,J., Eto,F., Nakane,C., Takao,K.,
            Miyakawa,T., Kabashima,K., Watanabe,M., Kahyo,T., Yao,I.,
            Fukuda,A., Ikegami,K., Konishi,Y. and Setou,M.
  TITLE     Tubulin Polyglutamylation by TTLL1 and TTLL7 Regulate Glutamate
            Concentration in the Mice Brain
  JOURNAL   Biomolecules 13 (5), 784 (2023)
   PUBMED   37238654
  REMARK    GeneRIF: Tubulin Polyglutamylation by TTLL1 and TTLL7 Regulate
            Glutamate Concentration in the Mice Brain.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7333)
  AUTHORS   Genova,M., Grycova,L., Puttrich,V., Magiera,M.M., Lansky,Z.,
            Janke,C. and Braun,M.
  TITLE     Tubulin polyglutamylation differentially regulates
            microtubule-interacting proteins
  JOURNAL   EMBO J 42 (5), e112101 (2023)
   PUBMED   36636822
REFERENCE   4  (bases 1 to 7333)
  AUTHORS   Wu,H.Y., Rong,Y., Bansal,P.K., Wei,P., Guo,H. and Morgan,J.I.
  TITLE     TTLL1 and TTLL4 polyglutamylases are required for the
            neurodegenerative phenotypes in pcd mice
  JOURNAL   PLoS Genet 18 (4), e1010144 (2022)
   PUBMED   35404950
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7333)
  AUTHORS   Bodakuntla,S., Yuan,X., Genova,M., Gadadhar,S., Leboucher,S.,
            Birling,M.C., Klein,D., Martini,R., Janke,C. and Magiera,M.M.
  TITLE     Distinct roles of alpha- and beta-tubulin polyglutamylation in
            controlling axonal transport and in neurodegeneration
  JOURNAL   EMBO J 40 (17), e108498 (2021)
   PUBMED   34309047
  REMARK    GeneRIF: Distinct roles of alpha- and beta-tubulin
            polyglutamylation in controlling axonal transport and in
            neurodegeneration.
            Erratum:[EMBO J. 2022 Jun 1;41(11):e111373. doi:
            10.15252/embj.2022111373.. PMID: 35642378]
REFERENCE   6  (bases 1 to 7333)
  AUTHORS   Mukai,M., Ikegami,K., Sugiura,Y., Takeshita,K., Nakagawa,A. and
            Setou,M.
  TITLE     Recombinant mammalian tubulin polyglutamylase TTLL7 performs both
            initiation and elongation of polyglutamylation on beta-tubulin
            through a random sequential pathway
  JOURNAL   Biochemistry 48 (5), 1084-1093 (2009)
   PUBMED   19152315
  REMARK    GeneRIF: TTLL7 performs both the initiation and elongation of
            polyglutamylation on beta-tubulin; the mechanism of binding of
            three substrates glutamate, ATP, and tubulin to the enzyme is a
            random sequential pathway.
REFERENCE   7  (bases 1 to 7333)
  AUTHORS   van Dijk,J., Rogowski,K., Miro,J., Lacroix,B., Edde,B. and Janke,C.
  TITLE     A targeted multienzyme mechanism for selective microtubule
            polyglutamylation
  JOURNAL   Mol Cell 26 (3), 437-448 (2007)
   PUBMED   17499049
REFERENCE   8  (bases 1 to 7333)
  AUTHORS   Ikegami,K., Heier,R.L., Taruishi,M., Takagi,H., Mukai,M.,
            Shimma,S., Taira,S., Hatanaka,K., Morone,N., Yao,I., Campbell,P.K.,
            Yuasa,S., Janke,C., Macgregor,G.R. and Setou,M.
  TITLE     Loss of alpha-tubulin polyglutamylation in ROSA22 mice is
            associated with abnormal targeting of KIF1A and modulated synaptic
            function
  JOURNAL   Proc Natl Acad Sci U S A 104 (9), 3213-3218 (2007)
   PUBMED   17360631
REFERENCE   9  (bases 1 to 7333)
  AUTHORS   Ikegami,K., Mukai,M., Tsuchida,J., Heier,R.L., Macgregor,G.R. and
            Setou,M.
  TITLE     TTLL7 is a mammalian beta-tubulin polyglutamylase required for
            growth of MAP2-positive neurites
  JOURNAL   J Biol Chem 281 (41), 30707-30716 (2006)
   PUBMED   16901895
  REMARK    GeneRIF: TTLL7 is a mammalian beta-tubulin polyglutamylase required
            for growth of MAP2-positive neurites
REFERENCE   10 (bases 1 to 7333)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC115889.13, AC165225.5 and BM899995.1.
            
            On Oct 24, 2017 this sequence version replaced XM_006502088.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.58455.1,
                                           SRR10223970.5896.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-223               AC115889.13        98412-98634
            224-422             AC115889.13        140047-140245
            423-554             AC115889.13        140409-140540
            555-676             AC115889.13        142651-142772
            677-744             AC115889.13        143792-143859
            745-903             AC115889.13        147369-147527
            904-1120            AC115889.13        150079-150295
            1121-1285           AC115889.13        155551-155715
            1286-1444           AC115889.13        159275-159433
            1445-1539           AC115889.13        161740-161834
            1540-1687           AC165225.5         137305-137452       c
            1688-1761           AC165225.5         137040-137113       c
            1762-1897           AC165225.5         135894-136029       c
            1898-1984           AC165225.5         132893-132979       c
            1985-2186           AC165225.5         127330-127531       c
            2187-2389           AC165225.5         123299-123501       c
            2390-2524           AC165225.5         122279-122413       c
            2525-2605           AC165225.5         119849-119929       c
            2606-2766           AC165225.5         105743-105903       c
            2767-2940           AC165225.5         99338-99511         c
            2941-7318           AC165225.5         83499-87876         c
            7319-7333           BM899995.1         2-16                c
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 72.28 cM"
     gene            1..7333
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /note="tubulin tyrosine ligase-like family, member 7"
                     /db_xref="GeneID:70892"
                     /db_xref="MGI:MGI:1918142"
     exon            1..223
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            224..422
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     CDS             398..3061
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /note="isoform 3 is encoded by transcript variant 3;
                     tubulin polyglutamylase TTLL7; tubulin--tyrosine
                     ligase-like protein 7"
                     /codon_start=1
                     /product="tubulin polyglutamylase TTLL7 isoform 3"
                     /protein_id="NP_001344146.1"
                     /db_xref="CCDS:CCDS89701.1"
                     /db_xref="GeneID:70892"
                     /db_xref="MGI:MGI:1918142"
                     /translation="MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITAN
                     VAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMG
                     EICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVK
                     PANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKI
                     FLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKW
                     FTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLG
                     FDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQ
                     KAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRH
                     MGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLL
                     EQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELD
                     ENEKELCQKRLDQVPYSLKHTSHCKIIQQSNSMRRSVSCPRSISAHLPSRGDVRPFSS
                     QQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTS
                     QTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDI
                     VKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFS
                     KSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQ
                     FLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL"
     exon            423..554
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            555..676
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            677..744
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            745..903
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1120
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1285
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1444
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1539
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1687
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1761
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1897
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1984
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2186
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2389
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2524
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2605
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2766
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2940
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..7319
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7297..7302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /note="hexamer: AATAAA"
     polyA_site      7319
                     /gene="Ttll7"
                     /gene_synonym="1110049N09Rik; 4921517B04Rik; C630030B20;
                     mTTLL7"
                     /note="major polyA site"
ORIGIN      
        1 atcggtagct accaccccct tgggcacgac agctgctcca gctgctcctg gtccggtcga
       61 gcttctaagc tctgaaccgc ggctgccccg ggggggtggg cgagctgagg tgatcccaag
      121 acgacacgcg gagtggaccg accggggagc cgccagccgc tggcggggag tgtgggccac
      181 caagagaccg gccactggat ccagccagag aggtgcctca gctgtagcat cccagcccaa
      241 aacttgtgtc gtgaaatcca agactttgac ccagataatg tgataggatc ttcctgtgac
      301 ttggggccat attctactta gatgaactcg gaacttccag ctttcccgag cgcttggcac
      361 tacaggcagg cttcttgctt gcttggtata cgttaccatg ccatctctgc ctcaagatgg
      421 ggttattcag ggatcatctc ctgtggatct gggtacagaa ttaccttacc aatgcacaat
      481 gaaaaggaaa gtgagaaaaa agaagaagaa gggaattatt acagctaatg ttgccggaac
      541 aaagtttgaa attgttcgtt tagtaataga tgagatggga tttatgaaaa ctcctgatga
      601 agatgagaca agcaatctta tctggtgtga tgctgctgtt cagcaggaga agattacaga
      661 cctgcagaac taccagagaa tcaaccattt tccaggaatg ggagagatct gtaggaagga
      721 tttcttagca agaaatatga cgaaaatgat caagtctcgg cctatggatt atacctttgt
      781 tccgagaacg tggattttcc cttctgaata tactcagttc caaaactatg tgaaagaatt
      841 gaagaaaaaa cggaagcaga aaacttttat agtaaaacca gctaatggtg caatgggtca
      901 tgggatttca ttgataagaa atggggataa agtcccttct caggatcatt tgatagttca
      961 agaatacatt gagaaacctt tcctaatgga aggctacaag tttgacttac ggatttatat
     1021 tttggttaca tcctgtgatc cattaaagat atttctctac catgatgggc ttgtgcgcat
     1081 gggcactgag aagtacatcc caccaaacga gtccaacttg acccagttat acatgcatct
     1141 gaccaactac tcagtgaaca aacacaatga acggtttgag cgcaatgaaa ctgaagacaa
     1201 aggcagcaag cggtcaatca agtggtttac ggaattccta caggccaatc aacatgatgt
     1261 cactaagttc tggagtgata tttcagagtt ggtggtaaag actctgattg tggcagaacc
     1321 tcacgtcctg cacgcctacc ggatgtgcag acccggtcag ccccccggga gcgaaagtgt
     1381 ctgcttcgaa gtcctggggt tcgacatctt gttggataga aagctcaagc catggcttct
     1441 ggagattaat cgagccccaa gctttggaac tgatcagaaa atcgattatg atgttaaaag
     1501 gggggttctg ctaaacgcgc tgaagctgct gaacatcagg accagcgata aaaggaaaaa
     1561 cttggccaag caaaaagcgg aggctcagag gaggctgtac ggtcaaaacc cagtaagaag
     1621 gctttcgcca ggctcctccg actgggaaca gcagaggcac cagctggaga ggcggaaaga
     1681 agagttgaaa gagagactcc ttcaagtacg aaagcaggtt tcgcaggaag aacatgaaaa
     1741 ccgacatatg gggaattaca ggagaattta tcctcctgaa gacaaagcac tgcttgagaa
     1801 gtatgaaggt ttgctggccg tcgccttcca gaccttcctc tcgggaagag cagcttcatt
     1861 ccagcgagaa atgaacaatc ctctgaaaaa gatgcgggag gaagatctcc tggatcttct
     1921 ggagcagtgc gaaatcgatg atgaaaagct gatggggaaa accggccggg tccgaggacc
     1981 gaagcccctg tgctgcatgc cagagtgtgc agaggtcacg aagaagcaga agtattacgg
     2041 tagcagtgac agcagctacg acagcagcag cagcagcagc aattcagagc tggatgagaa
     2101 cgaaaaagag ctgtgtcaga agagactaga ccaagtccca tatagcctta agcacaccag
     2161 tcactgcaag ataatccagc aatccaattc catgaggcgt tcggtcagct gccctcggtc
     2221 catctctgct cacttacctt ccagagggga tgtgcgcccc ttttcttctc aacaagtgat
     2281 accattagca agaccaacgt cagcgtcccg gtcacattcc ttaaaccgcg cttcatccta
     2341 cgcgagacac ctgcctcacg gcagtgacac cggctctacc aacactctga acgagtcttt
     2401 gcggcaattg aaaaccaaag agcaggaaga cgacctcacc agtcagacct tgtttgtcct
     2461 caaggacatg agaatccgct ttccggggaa gtcagacgca gagtcggagc tcctgataga
     2521 agatatcatg gataactgga agcattataa aacaaaagtg gcttcatatt ggctcataaa
     2581 attggactct gtaaaacaac ggaaagtttt ggacatagta aaatcaagta ttcgtacagt
     2641 tcttccaaga atctggaggg tccctgatgc tgaagaactg agtttatacc ggattttcaa
     2701 cagggtattt aatcgcctac tctggagtca tggccaagga ctgtggagct gtttctgtga
     2761 ttcaggatcc tcttgggaga gcatcttcag taagagccct gaggtggtga cccctctgca
     2821 gctccagtgc tgccagcgcc tggtggagct ttgcaagcag tgcctgctgg tggtttacaa
     2881 atacacaaca gaaacaagag gacccatctc aggcatcggt cctgactggg ggaactccag
     2941 gtatttgcta ccaggaagta cccaatttct tatgagatca ccactgtaca acatgaaata
     3001 taattcaccc ggaatgactc gctccaatgt tttgtttacg tcccgctatg gccgtttgtg
     3061 aatggaagat tgccatgggc tcgcttaata gcaattcatt tattttctcc agttgaacct
     3121 taaggaagtg acccttatat gctgttttat gtatatatgt catagaagtg tgtgaagtac
     3181 atacacacag gcactcaagt aacatataaa tatatatatt tattatatgt aagaagaatt
     3241 agcagaaaac tgaatataag atttaacctt tctggaaatg taataaacca tgttaaagtt
     3301 gtttacacaa atacgcaaat gtgtagaatt tgctaggttt ataattaatt acttcctact
     3361 agaaatgtta tttttgctgc agagtcgatc ctgaagctcc catcatggcg ttataaatta
     3421 ttttctagat aatagggctt tcgacttgag aaaacactga tagtatctta ttacctttct
     3481 cactattagt agttatttaa cagtgaaaca gaagacttag atgcctgggg ttggcttgat
     3541 gatcagatta ggatcaaaca gatttgttgt tactatagca cactgtaaag tactagaaaa
     3601 tagcccagcc tgactctcca acattttact cccctaggtg ggaatttagc tgcttattct
     3661 agtgcacatg ttcaataaag gtctggtaag atcatgaatc atgcaaaaat gagaggcaac
     3721 ccagtggatt aagagtaaaa tgcaaacatg ctttaattcg actcttcatg gggagtccca
     3781 tagagaagca agacgatggc agggacagaa atttggttgt gtgacttctg tgtcactttc
     3841 ttttggaaag tatatcattg atgggcccaa gtctctcaac attgagacca atttacctcg
     3901 caaagtccaa taagagtagt ttctcatttc accccagaaa agactccgaa gagaagagtc
     3961 cagtgagtcc cattgctcat cggtgtcatc tgttgggagt ctatattcaa agttctgtat
     4021 aaagttgtct tcctgtccag aggattcatg cttgtttttc agaccatcat gctctcattc
     4081 tgcttcatct tccttgccat ctgaaaacca gtgtgtgctc tcggttttcc atacaaatag
     4141 agacatatgc cctatggatt cttgaactca gaaaatacaa tatttgctga aacttccctc
     4201 cagtgatttt actcatacat gttaagtttc tgcaaaactc agaatatttg ccagcttctt
     4261 atatttttac gtttgttccc cataaatgaa gaaaggaccc caaagcagta agaggtcatt
     4321 tggcctggtc tcattcgaag ctggatggat ggtgacccga aattgtaaga tttaagtatt
     4381 ttgctttaaa aactaccttg ggagagtata cttttaaact gtaatattca tttatcacac
     4441 acattaccgg cagctactta agttctttat ggctgcctac ccacatgcat tccagtagag
     4501 actttttttt actggcaaca tcccatggaa ttttgtgtta tgtttttgaa agagaataaa
     4561 gcctccagat caagcagatg agtgagtgtg gtacttagga gtccatattg tggattcttc
     4621 aacatggcag agagttctta gaatcactga tgcaaatact ggaacacttc aggattttta
     4681 tggaaataat aaacaaagca gccccttttg gggtttattt gtagccatat ttgttttcaa
     4741 ttcatgattt aaaaatcaag gaggaataaa agaagaaaca caaaagtcat ggatatgtca
     4801 cttaaaatga ctgataagtg ataccaaccc agtttgttgg ccattcactt tgtcttaaat
     4861 cacaaatgat catttactca gatagagtat atactctgaa acttctgtca catactagga
     4921 aggaattttc attttaaaaa aggcgagggg agatgtccac attaggaatt ctcaccctag
     4981 aaacacacat taaaagctaa gagtaaatgg aaatagaaag actgccaagc atttgtgaaa
     5041 ggccattatt tccttatcta tccaagcaag gccttgtttt gagcactgga atttcattgc
     5101 ctacattttc ctgttgggac aagttgggta gggtgaagtg gtgcaggaag ctgtgtgctc
     5161 attatttatg tgcattacct ggggtgctgt ggactgtggt tgtcatagca ccatccccta
     5221 atgagcagcc acgggaagac atgataatga accgtggaag tctcaggagg ccacagacag
     5281 gtagtagggg tatagaatat tccttagctt ttatcaattt cttatgctta tgcttaaaac
     5341 acatctgcag cagagacagt gtaaatcttt aatagaagag aaggaaaatt ccagaagcta
     5401 gttaatttca ccagggtgtt tgtttgtttg tttgtttgtt tgtttgtttg tttttcctta
     5461 gaatggattt tggtttacct acaaggaaag gaatgagtat agacctgcag tatagatggc
     5521 tgtttggttc atggccaggt agaaatgctt ccccaaacta agatacagat gtcaccccaa
     5581 ttgtctaagt agtaacttgc ttgttagttt gtcctcaccc acaaacagaa tctaaaggaa
     5641 cagtttagtt ttcactcgag taagccagcc ttgaaggttt gaatctggta aaagaccctg
     5701 ttgcaatatg agtattaagt tgtatatgga gcacagggga agcaactccg ttgtctgtaa
     5761 aatgccaggg tttgcctaga ttagatgaag tttttgttac ttgtctctaa agttcaaacc
     5821 aaaccaaaac caaagccctt tctttcacct gctatttatg catacataga gaacaagtgc
     5881 ctcccagaca tcaggaaaat gattttcttc aaattctctc gttagcttgg atagtagatg
     5941 agactgggat catgcgatgt gaaattgtag atatctgaat aggaattcaa tgctttcttc
     6001 cttaacatgt caatgatatt ctgtgtaaac attatcttca atatgcaata cacactattc
     6061 aaaaatacca aaaatgatag ctgcatacat gccattactt aatcttctat aaagcactta
     6121 ttcattctta aatatatatt cttttaaaaa tagtaagtac ctcaaaagtg acagaaaatt
     6181 ccttccttct ctatttagca attgctagtt tacattccga atctcatgta cttggttcta
     6241 agcattaaac aacaataaaa atataactat acaattatcg ttcttgtgac ctggtgattt
     6301 ttggacattt acttttctct ttaaattgag agctttaagt gtaaagggcc atgatatcta
     6361 catattaatt attagatatc taattgtttt gaacaatttc aatgttggct tataagtaaa
     6421 tgaagactgt agaaatatat ttgtaaagca aatgattcct cacattgctt tctctctctc
     6481 ctgttctcct ttcaattcac ttagaagctt tagttgtgca tggacaaagt aaagaaagaa
     6541 atactgcctt ttaaatgctg gtcttcctgg aaattttgta gtggtgtgtt tttacccagg
     6601 caacactaac actttgatgg actattgggt caattcagtt tgccgagaac tttaggaaca
     6661 aggttgaagt ctgtatgctt aaagcacact cagtgtcctt ccgtgtgaag tgagatgcac
     6721 tttaatagtc ttgcaacaga aattgatgtg ttgaaacggc acctgcggag attgcttctg
     6781 tttctgaggg tctgccatga agtagtatgt ggtgtgttgg gagaaattta ctcaggtact
     6841 cactgtgata ccaagagggg tgtgtcatct tgggagatct gtataagact tctgctttat
     6901 caccacttat gaactcagaa gctcttgctt tgacaaacat atcactcaac aaactaagca
     6961 ctcctaaatt agtaagaagg cttgcctttg aaaagtggtc agataattat tctatttctt
     7021 tcaaaggcta ccagccattt ttggtccatg aggtcatata acaactactc tatgtgctat
     7081 agcatctgaa ttttaatgga gtctgaattt gagggtgaga gaatacattt gctaacacga
     7141 atcatgggtc aaattatccc tgtgtgcttt gcttcctgta tatgaatgca aatgttacaa
     7201 agatgcgctt gaaatgtcaa caaattgtgc agttggcatt gccatagcat gatctactgt
     7261 gtgacacctg tatggtgaac ccacttcagt tactataata aaaatgtcaa cacacgaaaa
     7321 aaaaaaaaaa aaa
//
