LOCUS       NM_001014974            8164 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4),
            mRNA.
ACCESSION   NM_001014974 XM_129771
VERSION     NM_001014974.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Ho,K.H., Candat,A., Scarpetta,V., Faucourt,M., Weill,S., Salio,C.,
            D'Este,E., Meschkat,M., Wurm,C.A., Kneussel,M., Janke,C.,
            Magiera,M.M., Genovesio,A., Meunier,A., Sassoe-Pognetto,M.,
            Brill,M.S., Spassky,N. and Patrizi,A.
  TITLE     Choroid plexuses carry nodal-like cilia that undergo axoneme
            regression from early adult stage
  JOURNAL   Dev Cell 58 (23), 2641-2651 (2023)
   PUBMED   37890489
REFERENCE   3  (bases 1 to 8164)
  AUTHORS   Wu,H.Y., Rong,Y., Bansal,P.K., Wei,P., Guo,H. and Morgan,J.I.
  TITLE     TTLL1 and TTLL4 polyglutamylases are required for the
            neurodegenerative phenotypes in pcd mice
  JOURNAL   PLoS Genet 18 (4), e1010144 (2022)
   PUBMED   35404950
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8164)
  AUTHORS   Wang,F., Tan,P., Zhang,P., Ren,Y., Zhou,J., Li,Y., Hou,S., Li,S.,
            Zhang,L., Ma,Y., Wang,C., Tang,W., Wang,X., Huo,Y., Hu,Y., Cui,T.,
            Niu,C., Wang,D., Liu,B., Lan,Y. and Yu,J.
  TITLE     Single-cell architecture and functional requirement of alternative
            splicing during hematopoietic stem cell formation
  JOURNAL   Sci Adv 8 (1), eabg5369 (2022)
   PUBMED   34995116
REFERENCE   5  (bases 1 to 8164)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8164)
  AUTHORS   van Dijk,J., Rogowski,K., Miro,J., Lacroix,B., Edde,B. and Janke,C.
  TITLE     A targeted multienzyme mechanism for selective microtubule
            polyglutamylation
  JOURNAL   Mol Cell 26 (3), 437-448 (2007)
   PUBMED   17499049
REFERENCE   7  (bases 1 to 8164)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   8  (bases 1 to 8164)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 8164)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 8164)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC152409.8.
            
            On Feb 2, 2019 this sequence version replaced NM_001014974.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085151.1, SRR10223971.522.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               AC152409.8         147998-148185       c
            189-355             AC152409.8         136877-137043       c
            356-1921            AC152409.8         129424-130989       c
            1922-2031           AC152409.8         128478-128587       c
            2032-2095           AC152409.8         128065-128128       c
            2096-2220           AC152409.8         124444-124568       c
            2221-2331           AC152409.8         123917-124027       c
            2332-2408           AC152409.8         123494-123570       c
            2409-2600           AC152409.8         122492-122683       c
            2601-2683           AC152409.8         122327-122409       c
            2684-2812           AC152409.8         122036-122164       c
            2813-2935           AC152409.8         121601-121723       c
            2936-3034           AC152409.8         121222-121320       c
            3035-3169           AC152409.8         120430-120564       c
            3170-3316           AC152409.8         119781-119927       c
            3317-3419           AC152409.8         114077-114179       c
            3420-3630           AC152409.8         113272-113482       c
            3631-3701           AC152409.8         113075-113145       c
            3702-3772           AC152409.8         112638-112708       c
            3773-8164           AC152409.8         108055-112446       c
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.54 cM"
     gene            1..8164
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="tubulin tyrosine ligase-like family, member 4"
                     /db_xref="GeneID:67534"
                     /db_xref="MGI:MGI:1914784"
     exon            1..188
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            189..355
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            356..1921
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..4031
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="tubulin polyglutamylase TTLL4; tubulin--tyrosine
                     ligase-like protein 4; protein monoglutamylase TTLL4"
                     /codon_start=1
                     /product="tubulin monoglutamylase TTLL4"
                     /protein_id="NP_001014974.1"
                     /db_xref="CCDS:CCDS15053.1"
                     /db_xref="GeneID:67534"
                     /db_xref="MGI:MGI:1914784"
                     /translation="MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQ
                     AHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNP
                     CYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTAN
                     KATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNN
                     SFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVL
                     DDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTE
                     AVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDS
                     AILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPP
                     SSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTED
                     ELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVV
                     RPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFK
                     ISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGK
                     KEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRP
                     LLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSN
                     KFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVV
                     VKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPL
                     DISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHF
                     TAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRIS
                     SRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLP
                     TSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSR
                     SNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS"
     exon            1922..2031
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2095
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2220
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2331
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2408
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2600
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2683
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2812
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..2935
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3034
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3035..3169
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..3316
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3419
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3630
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..3701
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3702..3772
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     exon            3773..8164
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4283..4288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="hexamer: TATAAA"
     polyA_site      4305
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="major polyA site"
     regulatory      8139..8144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="hexamer: AATAAA"
     polyA_site      8159
                     /gene="Ttll4"
                     /gene_synonym="4632407P03Rik; mKIAA0173"
                     /note="major polyA site"
ORIGIN      
        1 ccattccttt ctggccttag atctgtccgc tgggggcgcg ctctgtgtgg gacagaaagc
       61 ggcagagcgt gggtcgcgtg gcggagcttg gttgctaggg acacctgagg tcgcccgctc
      121 gcccagcagg cgcagcgaag aagcggcgcg gagccggcgt ccagtcgcgg cgaagtaggg
      181 taccgtagaa tactgcaact ctgaggcctc gcactgaaag tgattaattg ctgagccctg
      241 gatgtgatga actgctgact ggtgcttggc aggcactcct gtgaggtggt ggctgctgat
      301 gcagcgtggt tgggaagagg aagagtgccc tggaaactcg ggagacttca cacaagctga
      361 cggattccaa gatgcagctg gtgctagagg tgtgtggccc cttacctgag ccaggccatg
      421 aggccatgtt gccatgatgt gggcccctca tggcctcagc aggaacagag cactacagta
      481 ttggcctgcg ccgaggaaac agcttcaagc agagacatcc ctcaggcaca gtgtctgctt
      541 caccatctga gaaaccctcg gaggtcaaag tctggtctca ggcccatcag caggtgaagc
      601 caatctggaa actggaaaag aagcacgtag gcacactgtc agcagggctg ggcaccagtt
      661 tcttgggagt cccatcccag ccagcctatt tcctttgccc tagcacttta tgtagctctg
      721 ggaccacagc agtcattgca ggccacagca acccctgtta cctgcagtcc ctccctaact
      781 tgttcagcaa tactctgctg taccgccgca ccaacgtgag gcaaaaacca taccagcaac
      841 tggaatcttt ctgcttgcgt tccagccctt cagagaaaag atctttttct ctccctcaga
      901 agggtcttcc tgtcagtgtc actgccaata aagccacatc ttctacagtc ttccccatgg
      961 cccagcccat ggcaacttca cccactgatc catacctctc actggcagca gctggggaga
     1021 acccttcaag gaagagcctg gcctctgcta tctcagggaa gattgcatct ccactgtcct
     1081 ataagcccat gctgaacaac aactccttca tgaggccaaa tagcactaaa gtgcccttat
     1141 cacaagccac agatggcctg aagccagtat cctcacccaa gatccagcct gtctcatggc
     1201 atcattcagg gggtactggg gactgtgtac cccaacctgg tgaccataag gtgccccaaa
     1261 acattgctac tgtcttagat gatgtcactg cccctatcac cccatctatc cctagcaccc
     1321 tcaacatatc cacagccagt gttacctctt cccagtgcag tcagagtaac tttaggatgg
     1381 aggcacatcc ctgtggcctg gatgaaaacc cggattccca gagtgctact aaagaggtac
     1441 acttcactga ggctgtgagg aagttggctg aaaaaggctt ggagaagatg ccacgacaag
     1501 gctaccagtt tgaacaggct tgttttgtga accccagctt ccagtggggt cttctcaaca
     1561 gaagcaggcg gtggaaacct ctgatgggcc agcggtttcc tcaggaggat attggattag
     1621 acagtgcgat ccttcctggt acttcagaca ccttggggtt ggacagcaca gtcttctgta
     1681 ccaagaggat cagcattcac ctccttgcct cacatgtcca tgggctcaat cccagccctg
     1741 cctgtgggtc tgcagttgac ccccaggtac ttggagaaga cagagctcct gttccccctt
     1801 cttccctcca gcctcttggt gtagctgaag tggccactcg tctttcttct gtccatcttg
     1861 accagcctgg gaaggagcct gaggaagcca aggacctgaa ctcttgtact aagggtggtg
     1921 gttcagctac tgacctccag ccaaaccagg tggagcctga agatacagaa gatgaactgg
     1981 gagatggttt ggaagatagc tgcagccatg atgagaatga agaagaggaa ggagactcag
     2041 agtgctcttc tttaagtgtt gtctccccca gcgagtcagt ggcattaata tctcggaact
     2101 gtgtggattt gatgtccaaa tcccttccca atcatgagaa agttgtacgg ccagccctca
     2161 tctacagtct cttccccaat gtaaccccta ccatctattt tgggactcgg gatgaaagag
     2221 tggagaaact tccttgggaa cagaggaggc tgcttcggtg gaagatgagc acagtgaccc
     2281 ccaacattgt caagcagacc atcggtcggt cacacttcaa aatcagcaag agaaatgatg
     2341 actggctggg ctgctggggt caccacatga agtctcctgg ttttcgatcc attagagagc
     2401 atcagaagct aaaccacttc ccaggctcct tccagattgg gaggaaggac cggctgtggc
     2461 ggaacctgtc tcgcatgcaa agccgttttg gcaagaagga gttcagtttc tttcctcagt
     2521 cctttatcct gccccaggat tccaagctct tgcgcaaagc ctgggagagc agcagccgcc
     2581 agaagtggat tgtgaagcca ccagcatctg ctcgaggcat tggcattcag gtcattcaca
     2641 aatggagtca gctccccaag cgcaggcccc tcctggtaca gaggtatcta cacaagccct
     2701 acctcattag tggcagcaaa tttgatcttc ggatctatgt ttacgtgact tcttatgatc
     2761 ctctacggat ttacctcttt tcggatggac tcgtccgttt tgccagttgc aagtattccc
     2821 cttccatgaa gagtcttagc aacaagttca tgcacctgac caactacagt gtcaataaaa
     2881 agaacacaga ataccaggct aatgctgatg agactgcatg ccaagggcac aaatgggcat
     2941 tgaaagcttt gtggaactat ctgagccaga aaggaattaa tagtgatgcc atctgggaga
     3001 agataaagga tgttgtagtc aaaaccatca tctcgtcaga gccctatgtg actaacctac
     3061 tgaagttata tgtgcggcgc ccctatagct gtcatgagct ctttggtttt gacatcatgc
     3121 tggatgagaa cctgaaaccc tgggtcctag aagtcaacat ttctccaagc ctccattcca
     3181 actctcctct ggacatcagc atcaaaggcc agatgatccg agaccttctg aacctggctg
     3241 gctttgttct gcccaacatg gaggatatca tctctagttc cagcagcccc agcagctcca
     3301 gcggctccag caccagcttg ccaagctctc ctagagacaa atgtcaaatg actccagagc
     3361 acttcactgc acagaagatg aagaaagcct attacttgac ccagaaaatt cctgaccagg
     3421 acttctatgc gtccgtgctg gacgtcttga caccagatga tgttcgggtt ttggtggaga
     3481 tggaagatga gttttctcgt cgtggtcaat ttgaaagaat ttttccttcc cgaatctctt
     3541 ctcgatatct tcggtttttt gagcagccac gatatttcaa cattctcacc acccagtggg
     3601 aacagaagta ccatggaaac aagctcaaag gagtagatct gcttcggaat tggtgctaca
     3661 aaggcttcca cacggggatt gtctctgact cagccccact gtggtctctg ccaacatcgc
     3721 tcatgactac ctcaaagggt gatgggacac caaattccgc cagcaagtca cgaaagaaga
     3781 gcgcctctga gggaaccaca ctgtcctctg aagacaggtc aacacccaag tctaagaaga
     3841 gtcaagctgg cctgtctccc atatccagga agactttgtc ctcaaggagc aatgagaaca
     3901 ccagcaaaca gtccaagcgc tctaccccgg ggttacctgt cctcaagtac tctgggcaga
     3961 gttccagact ttctgctgct tctgcttccc agtcagtcac tgactcccgc ttgactgctg
     4021 tgagctcgtg actggtcctg cacaggagca ctcagctacc tcaagggaac cagcctgctg
     4081 accagcctga gcctctaccc cgcctccact ttgcccctca tcagagtatt tttgaagtgg
     4141 ttctattgta gatatgggca tctggagggc tggaagggcg atggtgatgg ggagaaggtg
     4201 aggaaggttc gccctctgtc accttactgt ctggctagca cctcatttct cagtagagaa
     4261 gacagtgaca gtcaagcagc tttataaagc agggtttggt ttctacactg agagccatgt
     4321 gtgatttatt tgaggccctg gtacagggct ggattgtagt ttaagaggag aaaacatgct
     4381 aaacattgaa gtctcttgtt ctgtaaatga gaggaattct ttgaaggtcc tgatttcctt
     4441 aggacctctg tcctctactt atccttgtct tgaagagaca gttttagaaa ccttcagact
     4501 tcttcaaaga caagcaagaa gaaaccatga caggctacac ccatttcctg gcctcctggc
     4561 cttgggtgac ctccacacca tatccattta agcttccacc ccatcagtag ctctccatct
     4621 tctgggagtc tctagggctc cagtgaagga tcctgatcat tttagttgct gctgtgtaga
     4681 ggagggtgga atgcgctgtg ctttcctcag tgtgggcaaa tcttgtagtc tcttttactt
     4741 ttagcagggt ttgtgccttg gtgggtcact cttatagaat aggcttctca tctctgacag
     4801 ctaatactca gtggcatcta cctcccccag gactcagtcc acactgagca ctgagtaaac
     4861 actgactgag tgaactgtgc attcagatct ccaaggacac tggcccctga agcactactg
     4921 ttctccccag tgtgaggaag tagagccaag gaaggaggcc aagatgagac agtttggatt
     4981 ggaagaggtt tcactagaac catggtcctc cttagcctag tgttccccag cactcacctc
     5041 ctgggacctg tggtctctaa ttttcacagc agaggggcct tgtatctgat aagcaagatt
     5101 tgtcctttgt gaccatctct gctagcattt tcctcattct tctgagtttt ggaaagagga
     5161 agacttcttt ctttccacat gactcctaga gcccttgacc agttggcatg gagccctgga
     5221 aagatgacct aactgggttc aaggtcacag aatgggtgaa catgacagtc agtcctaatg
     5281 ctgtgttgac agttgtgaag aaagctggat agcaaagtcc taagccgtgc ttcctgctta
     5341 atgataaagg cttcaaagtc agacagtgga gagtccaagc tgtactggaa aatctgttct
     5401 gatatcaaca gccaacaccc tcttgtcctg gttacagtat cctagtgcac atgtgacaaa
     5461 gaggcctgtt ttagctgatt cactccttca aagtagactc ttaattcctc agcctgcctc
     5521 tgagagtctc ccatcatagt ttccaactct gtagctgcag aaacaggcca acactgacca
     5581 gcccactagt tgcctggagt ccaccagact tggggacccc ttgatgagac acagcagtat
     5641 actggcagtt ctgttttgtg ttctggggtg atccagcctt gcctgggact tgtaatcttt
     5701 gcctcaaaaa tgccagattg tctgggtgtg ttgcgcccag ctaaatacac agtattcagt
     5761 cttcgttaca tttgaaggct ggtgaatctg tttgctagtt ggagaaaaag atagggcttg
     5821 tatatcacat ttcattttca agagtgtaat cttaagttat ttcacatgca tgttttagca
     5881 ctggctagtc aacaatagat agcattcggc tgaatgagga cttttaaagt aaaagctttt
     5941 gaaagtttta aatttgtaag tgtacttgaa tacaatttat cagtacatca gatgtttttc
     6001 caagttggta agttacccag cctctggagg aaggctcctg agacaaggaa taaagtcgtc
     6061 ttttccttta taatggaacg tgagacctac cctgcagggt tgtttagtaa gctaagccaa
     6121 agtattgggg attgcagttt ccgtttgttg gtttattgtc caaacattgt tctgtgaaag
     6181 aaatgtaaaa aaatctttcc ttgaaactga gtatgatagc tcataccctt aatcctagca
     6241 ctctgggaag gcaggagtgg gttgataaaa cctctccttt aaagaaaaga acagctacag
     6301 aagtctcaga agaggccaga gcagtgtcta tacctatcac ccttcactca gagcttgccg
     6361 aagcacaatt ttcataattc cctctaatct ttatcgttcc agcgggagtc tcttgtctgg
     6421 cttcatcttt atagcatccc aggaaaggga acattaaaca taaatgtgtg tgaagtgata
     6481 gtttggttct cccaagagcc ttgattggca aagaataaca agagtgtcta ggacagaaac
     6541 tcacagaata cagaccatga ggactgctgt gtggacctag caactaccag gggattgtgt
     6601 catttctggc ccatcgtcag ggtggcagct cttttgctga aagcgaccat agccaaagaa
     6661 ctttcttggg ctaatccaca cagtgatttc cacttctcct gggatacgac aatggttaag
     6721 gctgctaggg aacttgccct gttggcttct tgttagctct gaagaacccc tccgattact
     6781 gctccaacaa atgtctagtt tgctctgggc ctggaaacaa tggtttctag agagctgcat
     6841 cttatcgttt ggaattttgg ttcaagcctc ttcagcatgc ttagccttga acattaatct
     6901 gtagtccctg gggaccagcg tgtattcacc agcttcaggt cagtgggagc agcaagcagt
     6961 cagtagaatt tcctatgtga gtgaatgatc acagcagcat cggcaggcat gtgacattct
     7021 cagacctgtt tctgcttttg tcattagaat ggactaagat ttggtacatc agggattacc
     7081 acagaagtca acaaatttca gtttagtaac cagggatatc agtttcttag ctgtagtaac
     7141 ggggattaaa ttgtgtttaa aaagtgcctc ataactcaga taattcctaa aatatcaaat
     7201 gattccccca aaaccatttc ctcagagcag caggagtgcg gcgccaggaa ttgcagtact
     7261 cttagcttag cagttctgtg gcggagctgt cacagctcag ctagtgtgct caggcctgtg
     7321 gtcaccgttt gataggatca ggcttgtttt gttagtgttc ctaaactgtg caactatttg
     7381 catagcattt ccgatgtgtt gggtatagag ttaatctaga ctccatttga agtccacagg
     7441 aggatgtgtg caggttaaac ttgagcagca aatgcagctc catgttgcct aagggattga
     7501 acccctgtgt gctttggtat ctgagaagtt gtagaagcag tccccacaga ggctgcagaa
     7561 ctgctgtggt cctgtctgtg ggagattatt agagctgaag atttgatcaa gataattacc
     7621 ttaagagttt agacccctct ctgcttcccg agaggcacaa gtaaaactta acactaagtg
     7681 gtccagctgg aatgccagtg tctggaaatg gagagccatt ggtagggatg cgtgtattgc
     7741 tggcaaggtg cacaggtcca ctgatggttt ggtagtttct ttaaaagtag aacagcccat
     7801 gtggacagca gtcaatgaaa gaacaaatta tttagtagat actgtctaaa catctgcaag
     7861 gaaaaaccaa tgccacatct gggatgatct ctacagtatt gtgctgagtg aaagaagcca
     7921 gaagcaagcc tatatgcaga ttccagtcta gaaaatgaaa caaagcttac aaaacacagg
     7981 gattccttgg ggactacatc tggaggggac atgggattga ctgtaaagag gcacatggga
     8041 atattctttg tcttaattgt ggttgtggta acaactgtat atagttggcc gacctcaaaa
     8101 ttgtcctgtt gaaatgagta ttatacataa attttataaa taaagttgat tttctttaac
     8161 tttt
//
