LOCUS       NM_183017               3770 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tubulin tyrosine ligase-like family, member 12
            (Ttll12), mRNA.
ACCESSION   NM_183017
VERSION     NM_183017.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   van Dijk,J., Rogowski,K., Miro,J., Lacroix,B., Edde,B. and Janke,C.
  TITLE     A targeted multienzyme mechanism for selective microtubule
            polyglutamylation
  JOURNAL   Mol Cell 26 (3), 437-448 (2007)
   PUBMED   17499049
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK143496.1, AK150116.1 and AK084879.1.
            
            On Nov 26, 2009 this sequence version replaced NM_183017.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK084879.1, BC055368.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AK143496.1         2-36
            36-2483             AK150116.1         2-2449
            2484-3745           AK143496.1         2317-3578
            3746-3770           AK084879.1         3715-3739
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.4 cM"
     gene            1..3770
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /note="tubulin tyrosine ligase-like family, member 12"
                     /db_xref="GeneID:223723"
                     /db_xref="MGI:MGI:3039573"
     exon            1..209
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1967
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /note="TTL domain protein; inactive tubulin--tyrosine
                     ligase-like protein 12"
                     /codon_start=1
                     /product="tubulin--tyrosine ligase-like protein 12"
                     /protein_id="NP_898838.2"
                     /db_xref="CCDS:CCDS27706.1"
                     /db_xref="GeneID:223723"
                     /db_xref="MGI:MGI:3039573"
                     /translation="MEIQSGPQPGSPGRAERLNARLLDEFVSLHGPTLRASGVPERLW
                     GRLLHKLEHEVFDAGEMFGIMQVEEVEEAEDEAAREAQRKQPNPGGELCYKVIVTSES
                     GVRADDPNSIFLIDHAWTCRVEHARKQLQQVPGLLHRMANLMGIEFHGEVPSPEVVAL
                     VLEEMWKFNQTYQLAHGTAEEKVPVWYIMDEFGSRIQHSDMPSFATAPFFYMPQQVAY
                     TLLWPLRDLDTGEEVTRDFAYGEADPLIRKCMLLPWAPADMLDLSFSTPEPPAKYYQA
                     ILEENKEKLPLAISPVARPQGHVFRVHCDVQQVLGHLTHPRFTFTDSEADADIFFHFS
                     HFKDYMKLSQESPQVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTEL
                     PQFVSYFQHRERRGEDNHWICKPWNLARSLDTHVTNNLHSIIRHRESTPKVVSKYIES
                     PVLFLREDVGNVKFDIRYIVLLRSVRPLRLFAYDVFWLRFSNRPFALDDLDDYEKHFT
                     VMNYDPDVVLKQVHYNEFIPQFEKQYPEFPWSDVQAEIFKAFTELFQVACAKPPPMGL
                     CDYPSSRAMYAIDLMLNWDNHPDGKRVMQPQILEVNFNPDCERACRYHPSFFNDVFST
                     LFLDETDNCHVTRII"
     exon            210..379
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            380..578
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            579..738
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            739..872
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            873..949
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1066
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1261
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1373
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1525
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1607
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1676
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1815
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..3770
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3054
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
     regulatory      3201..3206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /note="hexamer: AGTAAA"
     polyA_site      3222
                     /gene="Ttll12"
                     /gene_synonym="D430005B17"
                     /note="major polyA site"
ORIGIN      
        1 gtccgcttaa atcctgcggt gggcggcgcc gtgctcttcc gagcactatg gagatccagt
       61 ccgggccgca gcctggcagt cccggccgcg ccgagaggct gaacgcgcgg ctgctggacg
      121 agttcgtgtc gctgcacggc cctacgctgc gcgcctcggg agtcccagag cgactatggg
      181 gccgccttct gcacaagctg gagcacgagg ttttcgatgc cggggagatg tttgggataa
      241 tgcaggtgga ggaggtggag gaggctgagg acgaggccgc cagggaggct cagaggaagc
      301 agcccaaccc tgggggcgag ctgtgctaca aggtcattgt gaccagtgag agcggcgtcc
      361 gggctgacga ccccaacagc atcttcctca tcgaccatgc ctggacgtgc cgtgtggagc
      421 atgcacgcaa acagctgcag caggtacctg gactcctgca ccggatggcc aacctcatgg
      481 gcatcgagtt ccatggggaa gtgcccagcc cagaggttgt ggccctagtg ctggaggaga
      541 tgtggaaatt caaccagacc tatcagctgg cccatgggac agccgaagag aaggtgccag
      601 tgtggtacat catggacgag tttggctcga ggatccagca ctcagacatg cccagcttcg
      661 ccactgctcc cttcttctac atgccccagc aggtggcata cacgctgctg tggcccctaa
      721 gggacctaga cacaggcgag gaggtgaccc gggactttgc ctacggagag gcagaccctc
      781 tgatccggaa atgcatgctg ctgccctggg cccccgccga catgctggac ctcagcttct
      841 ctacgcctga gcctcctgcc aagtactacc aggccatttt ggaggagaac aaggagaaac
      901 tgccgctcgc cattagccca gtggcacgtc cccagggcca cgtcttcaga gtccactgcg
      961 acgtgcagca ggtgctcggc caccttaccc accctcgctt caccttcact gatagcgagg
     1021 ctgatgccga catcttcttc cacttctcac acttcaagga ctacatgaaa ctcagccaag
     1081 agagcccaca ggtgctactc aaccagttcc cctgtgagaa tctgctgact gttaaggact
     1141 gtctggcctc catcgctcgc cgggctgggg gtcctgaggg tcccccctgg ctaccccgaa
     1201 ccttcaacct gcgcactgag ctgccccagt ttgtcagcta tttccagcac cgggagaggc
     1261 ggggtgaaga caaccactgg atctgcaagc cctggaacct ggcccgcagc ctggacactc
     1321 acgtcaccaa caacctccac agtatcattc ggcaccgaga gagcaccccc aaggttgtgt
     1381 ccaagtacat cgaaagtccc gtcttgttcc tccgagaaga tgtggggaac gtcaagtttg
     1441 acatccgcta cattgtgctg ctgcgctcag tgaggccgct gaggttgttt gcttacgatg
     1501 tgttttggct acgcttctcc aatcggccct tcgccctcga tgacctagac gactatgaga
     1561 agcatttcac cgtcatgaac tatgacccgg atgtggtgct gaagcaggtg cactataatg
     1621 agttcatccc tcagtttgag aagcagtacc cagagtttcc ctggagtgat gttcaggctg
     1681 agatcttcaa ggccttcacg gagctgttcc aagtggcatg tgccaagcca ccgcccatgg
     1741 gcctctgtga ctacccctca tcccgggcca tgtatgccat tgatctcatg ctcaactggg
     1801 acaaccaccc agatggaaag cgggtgatgc agccccaaat cctggaggtg aacttcaacc
     1861 ccgactgcga gcgagcctgc agataccacc cctcattctt caatgatgtc ttcagcactt
     1921 tgtttctgga cgagactgac aactgccatg tcacccgcat catctaggtg cccagagttc
     1981 tgcctgttct catagccagt tccagccggt tccagccggt ccagcctctg ctcacagaac
     2041 tgcctttgca gtcccaggct tctccctccc tccctctctc ccctccttcc ttcctcagcc
     2101 agagccttgg cctaagttat actcaaccct cctgagccgt gttcctgctc catatgtggg
     2161 atatcaggtc catgtgtggc agagctggac agtgagtggt gtcccaagga ctgggctccg
     2221 accagctgta cctggtctct gtacctctta acgccctctc ttctctgaag agtctgcagc
     2281 tcagctaagg gcttttctcc tggcatccag aaagttcgca gaccaagaaa tgttctgcag
     2341 aaacagggtt cgacgaggga agtgtttggg aacccggtat ccacaagggc cagggagtac
     2401 tctcagaatc tctgccctcc tgtcccatgg ctgctgcaag cagggtacgg gaaccctgtg
     2461 ttgcacccac aggccccaga acagccaagg ggaaacattt gccaacccgg ccttatgcct
     2521 cggcatcctc cgccagagcc ttcaccagca gacttctcag ccatgcctac cctgggtgtt
     2581 tcccttctgg gaagctgggc tatgcagagt cccatggtct ctgcatgttg gtacctcacc
     2641 aagtcccact gtgctcttgt ggtagacagg gtgctaggtt gctctagggc tgtgacattt
     2701 catggcagaa tccaagcagg agtggctgtg gaaatgccag agcggctaag atggctctgg
     2761 agagaacttc atgtgggaat caggctgcct cttgccaaag ttcagagtcc tagtgttaag
     2821 gttttgggtt tggttttcct tcaacctgca ggaaagacaa tgttacgtgg agttcctaat
     2881 tttgttgctg gaatttgaag tcagccacca ttgcttctgt aactgctggg cgctgggatc
     2941 ctgcccccag gaagttaaga gtgggcagga gctccttcca gatgtcctac tgttcgggtg
     3001 ggacactgtg gtggctgttg tacttgtgaa ggtaaaagtg accttctctt agcatgactg
     3061 gacatggttc ttcctgctca tctctgggct tgtaggccac ctgttaggga gcctgccccc
     3121 gccaccatca gggtcagggt agagacactt gtggtggtcg cacccctgga aagcggctgg
     3181 catctgccct tttatactgc agtaaaatac gcctcctttc cacctggtct tttctctttg
     3241 gggccctaga ggcctgggct gggctggcag ggcggctgtg tccctccaag gttgcaagca
     3301 gacccaacat gtcaccacgt gtgctttgag aaatgagaag gaaaagaaag acctccgagc
     3361 tccctaaggc agtctagctt cctagttaga gtcccagttc caagctggag aagagatctg
     3421 acctgaccag tccacactac caacccaagg gggcgttggg ggccgcaggg tctgaggctt
     3481 ggtcccacct ggtggccttg agaaatagca ttctctatgt tacctccttg ttctggagtg
     3541 gggatgagag gccataattc atgaggtagt caagaggctc tgggggctgg cagaggacca
     3601 gcaagacagc tcaccgagta aaaatgcctg ctgccaagcc tgaagacttc cctgggagct
     3661 acaggagaga gaaccaactc ccacgggttg tcctctgacc tccatgtgta taccatggga
     3721 catgtgactg gacaggcatg tacacacaaa aatataattt aaaagtcttc
//
