LOCUS       NM_027192               4562 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tubulin tyrosine ligase (Ttl), mRNA.
ACCESSION   NM_027192
VERSION     NM_027192.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Barnat,M., Benassy,M.N., Vincensini,L., Soares,S., Fassier,C.,
            Propst,F., Andrieux,A., von Boxberg,Y. and Nothias,F.
  TITLE     The GSK3-MAP1B pathway controls neurite branching and microtubule
            dynamics
  JOURNAL   Mol Cell Neurosci 72, 9-21 (2016)
   PUBMED   26773468
REFERENCE   3  (bases 1 to 4562)
  AUTHORS   Song,W., Cho,Y., Watt,D. and Cavalli,V.
  TITLE     Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated
            alpha-Tubulin in Injured Axons Is Required for Retrograde Injury
            Signaling and Axon Regeneration
  JOURNAL   J Biol Chem 290 (23), 14765-14775 (2015)
   PUBMED   25911101
  REMARK    GeneRIF: Tubulin-tyrosine ligase (TTL) is required to increase the
            levels of tyrosinated alpha-tubulin at the axon injury site and
            plays an important role in injury signaling.
REFERENCE   4  (bases 1 to 4562)
  AUTHORS   Bosson,A., Soleilhac,J.M., Valiron,O., Job,D., Andrieux,A. and
            Moutin,M.J.
  TITLE     Cap-Gly proteins at microtubule plus ends: is EB1 detyrosination
            involved?
  JOURNAL   PLoS One 7 (3), e33490 (2012)
   PUBMED   22432029
REFERENCE   5  (bases 1 to 4562)
  AUTHORS   Peris,L., Wagenbach,M., Lafanechere,L., Brocard,J., Moore,A.T.,
            Kozielski,F., Job,D., Wordeman,L. and Andrieux,A.
  TITLE     Motor-dependent microtubule disassembly driven by tubulin
            tyrosination
  JOURNAL   J Cell Biol 185 (7), 1159-1166 (2009)
   PUBMED   19564401
REFERENCE   6  (bases 1 to 4562)
  AUTHORS   Peris,L., Thery,M., Faure,J., Saoudi,Y., Lafanechere,L.,
            Chilton,J.K., Gordon-Weeks,P., Galjart,N., Bornens,M., Wordeman,L.,
            Wehland,J., Andrieux,A. and Job,D.
  TITLE     Tubulin tyrosination is a major factor affecting the recruitment of
            CAP-Gly proteins at microtubule plus ends
  JOURNAL   J Cell Biol 174 (6), 839-849 (2006)
   PUBMED   16954346
REFERENCE   7  (bases 1 to 4562)
  AUTHORS   Erck,C., Peris,L., Andrieux,A., Meissirel,C., Gruber,A.D.,
            Vernet,M., Schweitzer,A., Saoudi,Y., Pointu,H., Bosc,C.,
            Salin,P.A., Job,D. and Wehland,J.
  TITLE     A vital role of tubulin-tyrosine-ligase for neuronal organization
  JOURNAL   Proc Natl Acad Sci U S A 102 (22), 7853-7858 (2005)
   PUBMED   15899979
REFERENCE   8  (bases 1 to 4562)
  AUTHORS   Erck,C., MacLeod,R.A. and Wehland,J.
  TITLE     Cloning and genomic organization of the TTL gene on mouse
            chromosome 2 and human chromosome 2q13
  JOURNAL   Cytogenet Genome Res 101 (1), 47-53 (2003)
   PUBMED   14571137
REFERENCE   9  (bases 1 to 4562)
  AUTHORS   Lafanechere,L., Courtay-Cahen,C., Kawakami,T., Jacrot,M.,
            Rudiger,M., Wehland,J., Job,D. and Margolis,R.L.
  TITLE     Suppression of tubulin tyrosine ligase during tumor growth
  JOURNAL   J Cell Sci 111 (Pt 2), 171-181 (1998)
   PUBMED   9405300
REFERENCE   10 (bases 1 to 4562)
  AUTHORS   Ersfeld,K., Wehland,J., Plessmann,U., Dodemont,H., Gerke,V. and
            Weber,K.
  TITLE     Characterization of the tubulin-tyrosine ligase
  JOURNAL   J Cell Biol 120 (3), 725-732 (1993)
   PUBMED   8093886
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833780.6.
            
            On Aug 15, 2009 this sequence version replaced NM_027192.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024414.1, SRR7652917.799796.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-357               AL833780.6         151267-151623
            358-436             AL833780.6         154175-154253
            437-669             AL833780.6         161176-161408
            670-805             AL833780.6         166530-166665
            806-1075            AL833780.6         167294-167563
            1076-1219           AL833780.6         174314-174457
            1220-4562           AL833780.6         178261-181603
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.65 cM"
     gene            1..4562
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /note="tubulin tyrosine ligase"
                     /db_xref="GeneID:69737"
                     /db_xref="MGI:MGI:1916987"
     exon            1..357
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..1334
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /EC_number="6.3.2.25"
                     /codon_start=1
                     /product="tubulin--tyrosine ligase"
                     /protein_id="NP_081468.1"
                     /db_xref="CCDS:CCDS16719.1"
                     /db_xref="GeneID:69737"
                     /db_xref="MGI:MGI:1916987"
                     /translation="MYTFVVRDENSSVYAEVSRLLLATGYWKRLRRDNPRFNLMLGER
                     NRLPFGRLGHEPGLAQLVNYYRGADKLCRKASLVKLVKTSPELSESCSWFPESYVIYP
                     TNLKTPVAPAQNGIQLPVSNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGI
                     LISSEASELLDFIDSQGQVHVIQKYLERPLLLEPGHRKFDIRSWVLVDHQYNIYLYRE
                     GVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFEEFNQYLTSALN
                     ITLESSILLQIKHIIRSCLMSVEPAISTKHLPYQSFQLLGFDFMVDEELKVWLIEVNG
                     APACAQKLYAELCQGIVDIAISSVFPPPDTEQVPQQPAAFVKL"
     exon            358..436
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            437..669
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            670..805
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            806..1075
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1219
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..4562
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4535..4540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4553
                     /gene="Ttl"
                     /gene_synonym="2410003M22Rik; 2700049H19Rik"
                     /note="major polyA site"
ORIGIN      
        1 tgggggtggg cgcggcgtcc gggtggcgct cgccactcgg gcggtggcgc gggcgcccag
       61 ggcagacact agggtaatcc ggaggcgcgg ccctggaatt agaccgacag cggggctgag
      121 gctgaggctg gggcagtgtg cacgcggggc cgccgtgccc tggactcgtg tagcggctgc
      181 ggggcgcgct gcccttcgcc atgtacacct tcgtggtgcg cgacgagaat agcagcgtct
      241 acgccgaggt ctcccggctg ctgctggcca ccggctactg gaagaggcta cggcgagaca
      301 accccaggtt caacctgatg ctgggggaga ggaaccgact gcccttcggg agactaggtc
      361 acgagcctgg gctggcacag ctggtgaatt actaccgggg ggcggacaaa ctgtgccgca
      421 aagcttcctt agtaaagcta gtcaagacca gtccggaact gtctgagtcc tgctcatggt
      481 tccccgagtc gtatgtgatt tatccaacta acctcaagac gccagtcgct ccggcgcaga
      541 atggcatcca acttccggtc agtaactcca ggacagatga gagagaattc ttcttggctt
      601 cttataacag aaagaaagag gacggggagg gcaacgtttg gattgcaaag tcatcagctg
      661 gtgccaaagg tgagggcatc ctcatctcct cagaggcatc ggagcttctg gatttcatcg
      721 acagccaggg ccaagtgcac gtgatccaga aatacctcga gcgccctctg ctcctcgagc
      781 ccggccaccg caagtttgac attcgaagct gggtcctggt tgaccatcag tataacatct
      841 acctctatag agagggtgtg cttcgaactg cttcagaacc atatcatgtt gataatttcc
      901 aagataagac ctgccatctg accaatcact gcattcagaa agagtactca aagaactatg
      961 ggaaatacga agaagggaat gaaatgttct tcgaagagtt caatcagtac ctaaccagcg
     1021 ctctgaacat caccttagaa agtagcattt tgctgcaaat caaacacata ataaggagct
     1081 gcctcatgag tgtggagccc gccatcagca ccaaacacct cccttaccag agcttccagc
     1141 tcctcggctt tgacttcatg gtggacgagg agctgaaggt ctggctcatt gaagtcaacg
     1201 gcgccccagc gtgtgcccag aaactctatg cagaactgtg ccagggcatc gtggacatag
     1261 ccatatccag tgtcttccca cccccggaca cggagcaggt gccgcagcag ccggctgcgt
     1321 tcgtcaagct gtgaagcacc ggaaggcaca gagccacctt gggaaatgta cggggtcctg
     1381 ctccagagga aagagagaga actggactgc tccttgtcaa ggcatgctgg gggacagatg
     1441 tgcaggaaga gtgtgaagag atggcggctg tggaagagca gctgagctgc ccacagtgct
     1501 gtggagacct tgggaagctg gcgcaagcga ctgagaggga gagcccagca cttctctgaa
     1561 gggctgggac cagggatttg ttatggcgtg atctgatcct ttgagcggtt tctggccttg
     1621 gatggtactg ggaacagagc gccttctccc cgggatgcag cccaggggcc tctgaggggg
     1681 tgtctgctca cctgctgcag ctttagtgcc ttagtctgtg catggtgcag cctcactgga
     1741 cacagactgg accttagagc attttagagg ttgccacatg ttccctgcga cactatgtcc
     1801 cgctgaactc tggaccagca tgcatcacag tccgggctgt gccctggcct gtcagtgaag
     1861 gcctcttgct tgtcaccatc ccggtgggct atgagtgagc gcctgccagg ggtcctgtgg
     1921 acgccagtgt atgctgctca ctgcatgttt tagaaacaaa agccgcaggc ctgacgaatc
     1981 tgcaagcatc aaataagcat gagagagaga aaaacaaaaa caaaaccatg ataatcttac
     2041 tttacttaaa gggatgggtg gatgggtggg tgggagggag tctctgaaga gtcttgagtc
     2101 tcagttatct ttggtttaac cagaattctg aatgccgctt tgcaatttct ctccccttta
     2161 tccccccaca accccctccc cccacctttg aacctttgta gcaatccttt ctgggagtca
     2221 gagaaggtag aatccttttc ctcacactcc tcacctcata gctctcccca tgcggggcct
     2281 ccttggctcc aagggcctct gcaggagctg gagggccagg ttccattcca gaaagggccc
     2341 agtctgcata tgcatgtaag tgacaggaga gacgccctct cagtgagggt gtgcctgcct
     2401 gagtgagaaa gaagtatttt tggagctctg cactgcttct tttaagtgtc cacaaaagtg
     2461 taccatgaga tgttgtttct gggtcatggg tccattcata ttttatagac ttctagggag
     2521 cccgcttcca tggtggtgtc aggaagagtc tcgtgtattc agggtgagcg tgtgaacagg
     2581 cgcttctgaa gagtgtcctg gaaccacggc agacgaagcg aatctgccct tctccccaga
     2641 gcacagtgtg ttctttgctg gtgtaaccgt gtgcgtcttc atttgcattt acttcgtaca
     2701 ctctacagtc gagtgaaatg tccacagaag acctgctgaa gcacctcttc ctcagacagg
     2761 gtgaggtgtg ggcgcttgcg gcgaggaaag gaaagatgag acaattttag aatgtgcacc
     2821 agtgggtggc agcatgtgac agtatttact gaagcaagga aagttctaac tcatgggagc
     2881 ctggagttag agggcaaagg tgccagggtt acctggaatg tgggtaacag aggcggcacc
     2941 aagagcaaag ttaaacactc agtatgaaat cagttctaga ggcccagcac agctgacggg
     3001 gagtggtctg agaaggcagt gtgcacactc acttagaaag ggaggggggt gctgggtcct
     3061 cggctaggct aggtgggctg gtaatggaga aagcagggag gtctgtgggt tcttggagcc
     3121 aaatcacagg gacattcaag agtgtttagc tgttatggag ctggaaaagc agatgtcagt
     3181 aaatctttat gaattctgac aggttttgta ataagcctaa acaaatccat accctctgta
     3241 taaaggaagt gaggtgttac aaagtgggaa gggttgagtt ccttcctcac atagcttcaa
     3301 ccatttttat gcatttcctt cccaccaagt aggaagatcg gctggctttg agccgtttca
     3361 taccattata tggtatccct ggcacagatg gccatgcctg gcctcccagt cactcttcat
     3421 ggtcagttgg tgtaagtgct gcccatcacc cagggttggg gggttgtttg gagatgccaa
     3481 ttccacccat ggcactgtca ttagactgca caatgtcaca caagccaaat aacccactgt
     3541 gccttagtgt ctcgtggagg agacattacc tgacccaggg gctacctcag ggaggttttg
     3601 tgagaacaaa agagagtaag tggaagactc aggggccttt ggtaccaagg ttctcaatgc
     3661 cgaccgcatg ctacactctc catgaagtta agtaagtggc taacccttcc cacctgtggc
     3721 taattgaatc agaacctcag gtaacagtat gccttgaggt gccttgaacc attgctttgg
     3781 gaaagagggc aacacattat tatcttggaa gaatcactta aggattaggc tcaaaaaaaa
     3841 atactctttg aagcaagcaa ttctgtaaga acaatcagat gctgatatgt gtattcatag
     3901 tctaaatatt atccaagcag tgtctcagtt tgcttctcgt ccaggcatct gaaaaatcaa
     3961 acatgcgttc atttacctaa gcagtagtca agtccttggt agcaaatgta cacagacact
     4021 aatctataaa tctaatcata tatattagag ttatccatta attatgggag aaaagcccct
     4081 cccctcacac ttgacttcaa tggcaattat aaagtccatt ttgccaagaa tatataagtg
     4141 cctctgtgtt gtgaaacagc atggagctgg cctgcacagc agctgacagt gtgtgtcagt
     4201 agctctgtgg gatgacacat gcacaggaca tcggggagcg ctgggaatgg tctgtgaccc
     4261 acagggccag ccagtgatag tgatgctata aggtattctt ataaacaggc gtggttgtgt
     4321 tcttgggtga actcagtcct agtaccagtg tggcagggag gggacccatg aacaaaatga
     4381 caaggtgcca aagtgggttg catgtgggtt gtgggtgctg tcctgaggat tcagtagccg
     4441 atgtatgtgc ctccaatcct ggacacagag cctgcaccga agagggcatt tctgaaataa
     4501 ggcaactata ttgaatgttt taaatgcctg gagcattaaa gatatttcaa gtgttttgtg
     4561 tc
//
