LOCUS       NM_009445               2890 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Ttk protein kinase (Ttk), transcript variant 1, mRNA.
ACCESSION   NM_009445
VERSION     NM_009445.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Li,L., Sun,Y., Davis,A.E., Shah,S.H., Hamed,L.K., Wu,M.R.,
            Lin,C.H., Ding,J.B. and Wang,S.
  TITLE     Mettl14-mediated m6A modification ensures the cell-cycle
            progression of late-born retinal progenitor cells
  JOURNAL   Cell Rep 42 (6), 112596 (2023)
   PUBMED   37269288
REFERENCE   3  (bases 1 to 2890)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2890)
  AUTHORS   Fang,Q., Chen,X.L., Zhang,L., Li,Y.B., Sun,T.Z., Yang,C.X.,
            Chang,J.F., Yang,X.M. and Sun,F.
  TITLE     The essential roles of Mps1 in spermatogenesis and fertility in
            mice
  JOURNAL   Cell Death Dis 12 (6), 531 (2021)
   PUBMED   34031364
  REMARK    GeneRIF: The essential roles of Mps1 in spermatogenesis and
            fertility in mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2890)
  AUTHORS   Schukken,K.M., Zhu,Y., Bakker,P.L., Koster,M.H., Harkema,L.,
            Youssef,S.A., de Bruin,A. and Foijer,F.
  TITLE     Acute systemic loss of Mad2 leads to intestinal atrophy in adult
            mice
  JOURNAL   Sci Rep 11 (1), 68 (2021)
   PUBMED   33420244
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2890)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   7  (bases 1 to 2890)
  AUTHORS   Pritsker,M., Doniger,T.T., Kramer,L.C., Westcot,S.E. and
            Lemischka,I.R.
  TITLE     Diversification of stem cell molecular repertoire by alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 102 (40), 14290-14295 (2005)
   PUBMED   16183747
REFERENCE   8  (bases 1 to 2890)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2890)
  AUTHORS   Hwang,S.Y., Oh,B., Knowles,B.B., Solter,D. and Lee,J.S.
  TITLE     Expression of genes involved in mammalian meiosis during the
            transition from egg to embryo
  JOURNAL   Mol Reprod Dev 59 (2), 144-158 (2001)
   PUBMED   11389549
REFERENCE   10 (bases 1 to 2890)
  AUTHORS   Douville,E.M., Afar,D.E., Howell,B.W., Letwin,K., Tannock,L.,
            Ben-David,Y., Pawson,T. and Bell,J.C.
  TITLE     Multiple cDNAs encoding the esk kinase predict transmembrane and
            intracellular enzyme isoforms
  JOURNAL   Mol Cell Biol 12 (6), 2681-2689 (1992)
   PUBMED   1375325
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124011.4.
            
            On May 13, 2022 this sequence version replaced NM_009445.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058851.1, SRR17253011.4489224.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                AC124011.4         106186-106246
            62-215              AC124011.4         107258-107411
            216-429             AC124011.4         110687-110900
            430-536             AC124011.4         111213-111319
            537-680             AC124011.4         114568-114711
            681-795             AC124011.4         115115-115229
            796-868             AC124011.4         115371-115443
            869-963             AC124011.4         115534-115628
            964-1048            AC124011.4         117400-117484
            1049-1166           AC124011.4         118622-118739
            1167-1303           AC124011.4         122059-122195
            1304-1392           AC124011.4         124574-124662
            1393-1519           AC124011.4         126290-126416
            1520-1612           AC124011.4         128805-128897
            1613-1770           AC124011.4         133649-133806
            1771-1922           AC124011.4         133935-134086
            1923-2047           AC124011.4         134932-135056
            2048-2128           AC124011.4         136565-136645
            2129-2305           AC124011.4         139530-139706
            2306-2390           AC124011.4         140063-140147
            2391-2488           AC124011.4         140670-140767
            2489-2890           AC124011.4         143472-143873
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 45.61 cM"
     gene            1..2890
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /note="Ttk protein kinase"
                     /db_xref="GeneID:22137"
                     /db_xref="MGI:MGI:1194921"
     exon            1..61
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            62..215
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..2572
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /EC_number="2.7.12.1"
                     /note="isoform 1 is encoded by transcript variant 1; dual
                     specificity protein kinase TTK"
                     /codon_start=1
                     /product="dual specificity protein kinase TTK isoform 1"
                     /protein_id="NP_033471.2"
                     /db_xref="CCDS:CCDS52874.1"
                     /db_xref="GeneID:22137"
                     /db_xref="MGI:MGI:1194921"
                     /translation="MEAEELIGSSVTIDSIMSKMRDIKNKINEDCTDELSLSKICADH
                     TETVNQIMRVGNTPENWLNFLLKLEKNSSPLNDDLLNKLIGRYSQAIEVLPPDKYGQN
                     ESFARIQVRLAELKAIQEPDDARDYFQMARENCKKFAFVHVSFAQFELSQGNLKKSEQ
                     LLHKAVETGAVPLQMLETAMRNLHLQKKQLLPEEDKKSVSASTVLSAQEPFSSSLGNV
                     QNRSISCESRGQAGAARVLYGENLPPQDAEVRHQNPFKQTHAAKRSCPFGRVPVNLLN
                     SPDFYVKTDSSAVTQLTTRKGSGPDRDAILPGSRPRGSDSYELRGLKPIQTIYLKDSL
                     VSNEKSSELMSDLIALKSKTDSSLTKLEETKPEIAERRPMQWQSTRKPECVFQNPAAF
                     APLRHVPDVTPKADKESPPISVPKWLDPKSACETPSSSSLDDYMKCFKTPVVKNDFPP
                     ACPSSTPYSQLARLQQQQQQGLSTPLQSLQISGSSSINECISVNGRIYSILKQIGSGG
                     SSKVFQVLNEKKQINAIKYVNLEDADSQTIESYRNEIAFLNKLQQHSDKIIRLYDYEI
                     TEQYIYMVMECGNIDLNSWLKKKKSINPWERKSYWKNMLEAVHIIHQHGIVHSDLKPA
                     NFVIVDGMLKLIDFGIANQMQPDTTSIVKDSQVGTVNYMAPEAIRDMSSSRENSKIRT
                     KVSPRSDVWSLGCILYYMTYGRTPFQHIINQVSKLHAIINPAHEIEFPEISEKDLRDV
                     LKCCLVRNPKERISIPELLTHPYVQIQPHPGSQMARGATDEMKYVLGQLVGLNSPNSI
                     LKTAKTLYERYNCGEGQDSSSSKTFDKKRERK"
     exon            216..429
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            430..536
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            537..680
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            681..795
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            796..868
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            869..963
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1048
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1166
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1303
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1392
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1519
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1612
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1770
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1922
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2047
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2128
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2305
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2390
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2488
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2890
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2865..2870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /note="hexamer: AATAAA"
     polyA_site      2890
                     /gene="Ttk"
                     /gene_synonym="esk; Esk1; Mps1; PYT"
                     /note="major polyA site"
ORIGIN      
        1 aaacgtgttt ccggagaggg gttggggtgc tgctgtttgg gctgcagctt ggctttcaga
       61 gacgagttac aatttagaaa tggaggctga agagttaatt ggcagcagtg tgacgattga
      121 ttccatcatg agcaaaatga gagatattaa aaataagata aatgaagact gtactgatga
      181 gctaagcttg tctaaaatct gtgccgatca cacggaaact gttaaccaaa ttatgagggt
      241 tgggaacacc ccagagaact ggttgaattt cttgctgaaa ctagagaaaa acagctcacc
      301 tctaaatgac gatcttttaa ataagctgat tggtcggtat agtcaagcga ttgaagtact
      361 tcctccagat aaatacggcc agaatgagag ctttgctcga atacaagtga gacttgctga
      421 actaaaagct attcaagagc ctgatgatgc ccgtgactac ttccagatgg ccagggaaaa
      481 ctgcaagaag tttgcttttg tgcacgtatc ttttgcacag tttgaactgt ctcaaggcaa
      541 tcttaaaaaa agtgagcagc ttcttcataa agctgtagag actggggcgg tgccgctgca
      601 gatgctggag acggccatgc gtaacttaca cctccagaaa aagcagctgc ttccggagga
      661 ggacaagaag agtgtgtcag catcgacagt actaagtgcc caagagccgt tctccagctc
      721 acttggaaat gtacagaatc ggagcatcag ctgtgagtcc agaggacagg ctggggcagc
      781 cagggtttta tatggagaga acttgcctcc acaagatgcc gaagtgaggc atcaaaaccc
      841 cttcaagcag actcacgcag ctaaacggtc atgccccttt ggaagagtcc cagtcaatct
      901 tctaaacagc ccagatttct atgtgaagac agatagctca gctgtgacac agttaacaac
      961 aagaaagggg tcaggaccag accgagacgc gattctgccc ggctccagac cacgtggcag
     1021 tgattcctat gaactgagag gtttaaagcc cattcaaact atctatttga aagactcttt
     1081 ggtgtccaat gaaaagagtt ctgaacttat gtctgattta atagccttga agagtaaaac
     1141 agattcaagt ctaacaaaat tggaagaaac taagccagag attgcagaaa gaaggcccat
     1201 gcagtggcag tctaccagaa agcccgagtg tgtgttccag aaccctgctg cctttgcacc
     1261 cctgcggcac gttccagatg tcaccccgaa ggctgacaaa gagtcaccac caatatcagt
     1321 tcctaaatgg cttgatccaa agtctgcttg tgagacacct agtagcagct ccttggatga
     1381 ttacatgaaa tgttttaaga ctccagttgt aaagaatgac tttccacctg cctgtccatc
     1441 atcaacacct tacagccagc ttgcccgcct ccagcagcaa cagcagcagg gactcagcac
     1501 tcctcttcaa agcttgcaga tttcaggttc ttcatcaata aatgaatgca tttcagttaa
     1561 cggaagaatt tattccatat taaagcagat aggcagtgga ggttccagta aggtgtttca
     1621 ggtattgaat gagaaaaaac agataaacgc tatcaaatat gtgaacctag aagacgccga
     1681 tagccaaact attgagagct accgcaacga gatagcgttt ttgaacaaac tacagcaaca
     1741 cagtgataag atcatccgcc tctatgatta tgaaatcacc gagcagtaca tctacatggt
     1801 aatggaatgt ggaaacattg acctaaatag ttggcttaaa aagaaaaaat ccatcaatcc
     1861 atgggaacgc aagagctact ggaaaaacat gttggaggca gtacacataa tccatcagca
     1921 tggtattgtt catagtgatc tgaagcctgc taactttgtg atagtggatg gaatgctaaa
     1981 gctaattgat tttgggattg caaaccaaat gcagccagac acaacaagca ttgttaaaga
     2041 ttctcaggtt ggcacagtta actatatggc cccagaagca atcagagaca tgtcttcttc
     2101 aagagaaaat tcgaaaatca ggaccaaggt aagtcccaga agtgatgtct ggtccttggg
     2161 gtgcattttg tactacatga cttatgggag gacgccattt cagcacatca tcaatcaggt
     2221 ctctaaactg cacgccataa tcaaccctgc tcatgagatt gaatttcccg agatttcgga
     2281 aaaagatctt cgagacgtgt taaagtgctg tttagtgagg aaccctaaag agaggatatc
     2341 tatccctgag cttctcacac atccgtatgt tcaaattcag ccccatccag gcagccaaat
     2401 ggctagggga gccactgatg aaatgaaata tgtgttgggt caacttgttg gtctgaattc
     2461 tcctaactcc atcttgaaaa ctgcaaaaac tttgtatgaa cgttataatt gtggtgaagg
     2521 tcaagattct tcgtcatcca agacttttga caaaaagaga gaaagaaagt gatgcacagc
     2581 tacgtacaaa ccaagaacac tagattgttt cctctgccat actcttgaat ctctgaggaa
     2641 atctaccagt tggaaacaac ctcacctgga ttttatcagt taaaaaaaca aacaaacaaa
     2701 acttcagtag attatcctca aaaggaagct gtaaagttaa ccactcatag cactgtgtat
     2761 attaaattat agagttgtgc ttttctttta tgcttttctg taaatctgct aatgttttac
     2821 gtttagaaca gtgaatgata gctggaatgt tgaagagctc tgtaaataaa gcgtcaccac
     2881 agttccagaa
//
