LOCUS       NM_001047604            4489 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus tetratricopeptide repeat domain 21B (Ttc21b),
            transcript variant 1, mRNA.
ACCESSION   NM_001047604 XM_130319 XM_899617 XM_899621 XM_899628 XM_980434
            XM_991856
VERSION     NM_001047604.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Wang,W., Silva,L.M., Wang,H.H., Kavanaugh,M.A., Pottorf,T.S.,
            Allard,B.A., Jacobs,D.T., Dong,R., Cornelius,J.T., Chaturvedi,A.,
            Swenson-Fields,K.I., Fields,T.A., Pritchard,M.T., Sharma,M.,
            Slawson,C., Wallace,D.P., Calvet,J.P. and Tran,P.V.
  TITLE     Ttc21b deficiency attenuates autosomal dominant polycystic kidney
            disease in a kidney tubular- and maturation-dependent manner
  JOURNAL   Kidney Int 102 (3), 577-591 (2022)
   PUBMED   35644283
  REMARK    GeneRIF: Ttc21b deficiency attenuates autosomal dominant polycystic
            kidney disease in a kidney tubular- and maturation-dependent
            manner.
            Erratum:[Kidney Int. 2023 Feb;103(2):429. doi:
            10.1016/j.kint.2022.11.015.. PMID: 36681463]
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Bumann,E.E., Hahn Leat,P., Wang,H.H., Hufft-Martinez,B.M., Wang,W.
            and Tran,P.V.
  TITLE     Genetic Interaction of Thm2 and Thm1 Shapes Postnatal Craniofacial
            Bone
  JOURNAL   J Dev Biol 10 (2), 17 (2022)
   PUBMED   35645293
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4489)
  AUTHORS   Wang,W., Pottorf,T.S., Wang,H.H., Dong,R., Kavanaugh,M.A.,
            Cornelius,J.T., Dennis,K.L., Apte,U., Pritchard,M.T., Sharma,M. and
            Tran,P.V.
  TITLE     IFT-A deficiency in juvenile mice impairs biliary development and
            exacerbates ADPKD liver disease
  JOURNAL   J Pathol 254 (3), 289-302 (2021)
   PUBMED   33900625
  REMARK    GeneRIF: IFT-A deficiency in juvenile mice impairs biliary
            development and exacerbates ADPKD liver disease.
REFERENCE   4  (bases 1 to 4489)
  AUTHORS   Allard,B.A., Wang,W., Pottorf,T.S., Mumtaz,H., Jack,B.M.,
            Wang,H.H., Silva,L.M., Jacobs,D.T., Wang,J., Bumann,E.E. and
            Tran,P.V.
  TITLE     Thm2 interacts with paralog, Thm1, and sensitizes to Hedgehog
            signaling in postnatal skeletogenesis
  JOURNAL   Cell Mol Life Sci 78 (7), 3743-3762 (2021)
   PUBMED   33683377
  REMARK    GeneRIF: Thm2 interacts with paralog, Thm1, and sensitizes to
            Hedgehog signaling in postnatal skeletogenesis.
REFERENCE   5  (bases 1 to 4489)
  AUTHORS   Brooks,E.R., Islam,M.T., Anderson,K.V. and Zallen,J.A.
  TITLE     Sonic hedgehog signaling directs patterned cell remodeling during
            cranial neural tube closure
  JOURNAL   Elife 9, e60234 (2020)
   PUBMED   33103996
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4489)
  AUTHORS   Stottmann,R.W., Tran,P.V., Turbe-Doan,A. and Beier,D.R.
  TITLE     Ttc21b is required to restrict sonic hedgehog activity in the
            developing mouse forebrain
  JOURNAL   Dev Biol 335 (1), 166-178 (2009)
   PUBMED   19732765
  REMARK    GeneRIF: Data characterize the forebrain phenotype caused by loss
            of Ttc21b, a gene we identified in an ENU mutagenesis screen as a
            novel ciliary gene required for retrograde intraflagellar
            transport.
REFERENCE   7  (bases 1 to 4489)
  AUTHORS   Patterson,V.L., Damrau,C., Paudyal,A., Reeve,B., Grimes,D.T.,
            Stewart,M.E., Williams,D.J., Siggers,P., Greenfield,A. and
            Murdoch,J.N.
  TITLE     Mouse hitchhiker mutants have spina bifida, dorso-ventral
            patterning defects and polydactyly: identification of Tulp3 as a
            novel negative regulator of the Sonic hedgehog pathway
  JOURNAL   Hum Mol Genet 18 (10), 1719-1739 (2009)
   PUBMED   19223390
REFERENCE   8  (bases 1 to 4489)
  AUTHORS   Tran,P.V., Haycraft,C.J., Besschetnova,T.Y., Turbe-Doan,A.,
            Stottmann,R.W., Herron,B.J., Chesebro,A.L., Qiu,H., Scherz,P.J.,
            Shah,J.V., Yoder,B.K. and Beier,D.R.
  TITLE     THM1 negatively modulates mouse sonic hedgehog signal transduction
            and affects retrograde intraflagellar transport in cilia
  JOURNAL   Nat Genet 40 (4), 403-410 (2008)
   PUBMED   18327258
REFERENCE   9  (bases 1 to 4489)
  AUTHORS   Hou,Y., Qin,H., Follit,J.A., Pazour,G.J., Rosenbaum,J.L. and
            Witman,G.B.
  TITLE     Functional analysis of an individual IFT protein: IFT46 is required
            for transport of outer dynein arms into flagella
  JOURNAL   J Cell Biol 176 (5), 653-665 (2007)
   PUBMED   17312020
REFERENCE   10 (bases 1 to 4489)
  AUTHORS   Herron,B.J., Lu,W., Rao,C., Liu,S., Peters,H., Bronson,R.T.,
            Justice,M.J., McDonald,J.D. and Beier,D.R.
  TITLE     Efficient generation and mapping of recessive developmental
            mutations using ENU mutagenesis
  JOURNAL   Nat Genet 30 (2), 185-189 (2002)
   PUBMED   11818962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844526.5 and AL928586.10.
            
            On Oct 27, 2022 this sequence version replaced NM_001047604.2.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ011164.1, SRR9219379.30971.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AL844526.5         51092-51216         c
            126-255             AL844526.5         46681-46810         c
            256-366             AL844526.5         46472-46582         c
            367-533             AL844526.5         43973-44139         c
            534-656             AL844526.5         41886-42008         c
            657-814             AL844526.5         37209-37366         c
            815-899             AL844526.5         34101-34185         c
            900-998             AL844526.5         32365-32463         c
            999-1191            AL844526.5         30799-30991         c
            1192-1289           AL844526.5         30151-30248         c
            1290-1490           AL844526.5         29621-29821         c
            1491-1620           AL844526.5         25867-25996         c
            1621-1778           AL844526.5         23560-23717         c
            1779-2000           AL844526.5         21309-21530         c
            2001-2239           AL844526.5         20484-20722         c
            2240-2312           AL844526.5         18654-18726         c
            2313-2423           AL844526.5         18093-18203         c
            2424-2562           AL844526.5         17232-17370         c
            2563-2669           AL844526.5         11763-11869         c
            2670-2858           AL844526.5         4570-4758           c
            2859-2969           AL844526.5         3232-3342           c
            2970-3051           AL844526.5         2030-2111           c
            3052-3202           AL928586.10        99946-100096        c
            3203-3364           AL928586.10        99596-99757         c
            3365-3560           AL928586.10        96141-96336         c
            3561-3785           AL928586.10        90032-90256         c
            3786-3906           AL928586.10        86733-86853         c
            3907-3974           AL928586.10        84627-84694         c
            3975-4489           AL928586.10        82802-83316         c
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 39.08 cM"
     gene            1..4489
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /note="tetratricopeptide repeat domain 21B"
                     /db_xref="GeneID:73668"
                     /db_xref="MGI:MGI:1920918"
     exon            1..125
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..4052
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /note="isoform a is encoded by transcript variant 1;
                     tetratricopeptide repeat-containing hedgehog modulator 1;
                     tetratricopeptide repeat protein 21B; TPR repeat protein
                     21B; line 158; intraflagellar transport 139 homolog"
                     /codon_start=1
                     /product="tetratricopeptide repeat protein 21B isoform a"
                     /protein_id="NP_001041069.1"
                     /db_xref="CCDS:CCDS16076.1"
                     /db_xref="GeneID:73668"
                     /db_xref="MGI:MGI:1920918"
                     /translation="MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHA
                     YGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQ
                     RKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPY
                     AKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKK
                     MKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNA
                     LDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQM
                     ILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMG
                     KSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLE
                     VVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDT
                     EAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYH
                     LIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVE
                     VHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTE
                     QPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEA
                     IVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELL
                     LRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQA
                     RELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCE
                     TDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVY
                     HLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGL
                     HFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGT
                     SPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFT
                     EIAASEKDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLAD
                     IYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQ
                     TNPAVGYKLAFNYLKAKRYVDAIDVCHQVLEAHPTYPKIRKDILDKARASLRP"
     exon            126..255
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            256..366
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            367..533
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            534..656
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            657..814
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            815..899
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..998
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1191
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1289
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1490
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1620
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1778
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..2000
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2239
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2312
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2423
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2562
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2669
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2670..2858
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2969
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3051
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..3202
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3203..3364
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3365..3560
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3785
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3906
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3907..3974
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..4489
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4472..4477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /note="hexamer: AATAAA"
     polyA_site      4489
                     /gene="Ttc21b"
                     /gene_synonym="2410066K11Rik; aln; mKIAA1992; Thm1"
                     /note="major polyA site"
ORIGIN      
        1 gtgctgcacg ccgcgtccct tagggtttga gggcccggga agtgggtctg aacccctaac
       61 cctgcggcgg gcagcggtgg gcaccctccg ggtagccggc ggccatggac tcccaggggc
      121 tgaagacgct gattaactac tattgtcaag agcgctatta tcaccacgtg ctccttgttg
      181 ccagtgaagg gatgaagaag tatagcagcg acccagtctt ccgattttac cacgcctacg
      241 gcacactgat ggagggtaaa gcgcaagaag cccttcggga gtttgaggcc attaaaaaca
      301 aacaagacgt gtcactgtgt tctctgatgg cgctgatgta tgtccataaa atgagcccca
      361 atccagacag agaagctatc ctggaattag ataccaaaat gaaggagcaa cgcaaggagg
      421 ctggacgcaa agccctgtac catgcgggcc tgtttttatg gcacattggt cgtcatgaca
      481 aggcgagaga atatattgac agaatgtcaa aaatgccaca tgatagcaat gaggggccga
      541 ttttgaaagc gtggcttgat attacaagag ggaaggaacc ttatgctaaa aaagccctgc
      601 ggtattttga ggagggatta caagatggaa atgatatttt cgctcttctg ggtaaggttc
      661 tgtgccttga gattcgacag aattattccg gagctctgga gaccgtgagc cagataattg
      721 taaactttcc aagcttcctt cctgcctttg agaagaaaat gaaattacaa ctggctttac
      781 aggattggga tcagacagtg gagacagcac aaaggctgct gcttcaagac aatcacaacg
      841 tggaggcgct gaggatgctg gctctgtatt atctgtgtag ggaaggggac gtagagaagg
      901 ctgctaccaa gctggaaaat ttaggaaatg cattggatgt catggaacca cagaatgctc
      961 aactttttta taagattaca ctagccttca gcagaacgtg tggacgtaat caactcattc
     1021 tccagaaagt tcaaagtttc ctagaaaaag catttagttt aactccccag caagcagaaa
     1081 ttgctacaga gctcggctac caaatgattc tccaaggcaa ggtcaaggag gcctggaagt
     1141 ggtacaggac cgccatgacg ctgaatgaga gcaacatctc tgctgtcacc ggacttatcc
     1201 gatgtcagtt aatagagggg cagttgcaag atgcagacca gcagttggag ttcttcagtg
     1261 aattccagca gtcaatgggg aaatctgcgg aattaatgta tttgcatgcg gttcttgcta
     1321 cgaaaaaaaa taatcgtcag gatgaagtta taaatttgtt gaatgatgtt gtgaatactc
     1381 acttttcaca cttggaagac ctcccgctcg gcatacagta ttttgagaag ctcaaccctg
     1441 acttcttact agaagttgtt accgagtatc tgaatctctg tccaattcag cctgcaggtc
     1501 ctggacaacc tctttctcca gttctcagac gttgttcttc agtcctggag acgattataa
     1561 gaagtgtacc aggtcttcca caagctgtct tcctaatggc caaagtgaag tatttgtcag
     1621 gtgacactga agcggcatac aacaatctgc agcactgcct cgagcacagc ccctcctatg
     1681 cggaggctca cctcctgatg gcacaggtgt acctgtccca agacaaagtc aagctgtgtt
     1741 cgcagtcgct tgaactttgt ctgagctaca attttaatgt gagagagtac ccattatatc
     1801 atttaatcaa agcacagtca caaaagaaaa tgggggaagt agcagaagca attaaaactc
     1861 tgcacatggc aatgaacctg ccaggaatga ggagaagcag agcctcctcg aaatcaaagc
     1921 acagaactga ggtggacgcg agccatcggt tatccatctt cctggagttg gtggaggtcc
     1981 accgcttaaa tggagaacag catgaggcag caaaggtttt acaggatgcc atccatgagt
     2041 tttcgggaac ttgtgaagaa ttacgtgtca ctattgctaa tgcagacctg gctctggccc
     2101 aaggagatac ggatcgtgca ttaagcatgc ttcgaaatgt cacaaccgaa cagccttatt
     2161 ttatagaggc caaagaaaag atggcagata tttatctgaa gcataggaaa gagaaaatgt
     2221 tgtatatcac ttgttacaga gaaattgctg agagaatgcc cagtccccgg tctttcctgc
     2281 tcctcggcga tgcgtacatg aacattcagg agccagaaga agccatagtg gcctatgagc
     2341 aagcactcaa tcagaacccc aaagatggaa cactggcaag aaaaattgga aaagcacttg
     2401 tcaaaactca caattactca aaggcaatca cctattatga agcggctctg aaaagtggac
     2461 agcagaattg cctttgctat gacctggccg agctgctact gagactgaag ctgtacgaga
     2521 aagcagagaa agtgctccag cactctctag ctcatgagcc tgtaagtgag ttgtcagctc
     2581 tcatggtgga tgggcggtcc caagtccttc ttgcaaaagt ttactctaaa atggagagac
     2641 ccagcgacgc gattgccgca ttacagcagg ctcgagagct gcaggctcgg atattgaagc
     2701 gagttcagat ggagcagcca gatgcagttc cttcacagaa acacttcgca gctgaaattt
     2761 gtgcagagat tgcgaaacac tccgccgctc agcgagacta tgagaaagca attacatttt
     2821 atagagaagc cctggttcat tgtgaaacag acagtaagat aatgttggaa ctggcgcagt
     2881 tatacctggc ccaagaagac cttgacgcct ccctgcgaca ctgtgcgctg cttctccaga
     2941 gggaccagga caatgaacct gccaccatgt tgatggccga cctcatgttc agaaagcaag
     3001 actatgaaca agcagtgtat catctgcagc agcttttaga ccggaaacca gataatttta
     3061 tgactttgtc ccgtttgatt gatctcctga gaagatgtgg gaaacttgag gatgttccga
     3121 gatttttctt gatggctgag aaacacaact ccagaacaaa attggagcca ggatttcagt
     3181 actgtaaagg attacatttt tggtatactg gagaaccaaa tgatgccctt cgacatttta
     3241 ataaagctcg gaaagatagt gactggggtc agaatgcact ttataatatg atagaaatct
     3301 gcctaaatcc agacaatgaa actattggag gtgaagtgtt tgaaaacctg aatggagacc
     3361 tgggcacttc ccctgagaag caggagtctg tgcagttagc agtgaggaca gcagaaaagc
     3421 tccttaagga actgaagcct cagaccgtcc agggccgcct gcaactccgc ataatggaaa
     3481 actgctgctt gatggccacc aagcagaagt ccagtgtgga gcaggcactg aacaccttca
     3541 ccgaaatcgc agcctccgag aaggatcata tcccagctct cttgggaatg gcaacggctt
     3601 atatgatctt gaaacagact ccaaaagcca gaaaccagct gaagcggatt gcaaaaatgc
     3661 cttggaatcc tattgaagcc gaggatctgg agaagagctg gctgctgctt gctgatattt
     3721 atatccagtc agcaaaatat gacatggcag aggaattact aaagcggtgt ctctgccata
     3781 accggtcctg ctgcaaagct tatgaatata tggggtacat tatggagaaa gagcaagcat
     3841 acacagatgc ggccttcaac tatgagatgg catggaaaca tagcaaccag acaaatcctg
     3901 cagtgggata caaattggca tttaattacc taaaagcaaa acgatatgtg gatgcaattg
     3961 acgtatgtca ccaggttctt gaagcacatc caacttaccc caaaatcaga aaggatatac
     4021 ttgataaggc gcgtgcatct ttaagacctt gaaaatgatt tttactgaag tgttgggcaa
     4081 gagatgacag tggtccctca gctttcacag tctttgcagt tgacacaggc tccagctgac
     4141 agtgtgacag aagtgtgtcc tgccttctcc agcagaggct gctgctgtcc gtggacaaca
     4201 ctggtctgtg gcatccttgg tggagtggct gggtgagagc tcatctttct cccttccatt
     4261 aggcacaatg gtagttctgt gataaagctt aactcgtttc tacagaggat atttttgtta
     4321 aagtctgggt gatatatctt ttctgaagag tattagattt tccaacaatt ctatttaaag
     4381 tctactgtca tctgttaatt ttctattata gtttgtgata taatctcatg taatttatga
     4441 aaaatgaata catattttaa atgtatattt taataaagat ttttgtgaa
//
