LOCUS       NM_080788              10952 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tau tubulin kinase 2 (Ttbk2), transcript variant 1,
            mRNA.
ACCESSION   NM_080788 XM_619802
VERSION     NM_080788.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Nguyen,A. and Goetz,S.C.
  TITLE     TTBK2 controls cilium stability by regulating distinct modules of
            centrosomal proteins
  JOURNAL   Mol Biol Cell 34 (1), ar8 (2023)
   PUBMED   36322399
REFERENCE   3  (bases 1 to 10952)
  AUTHORS   Khang,R., Jo,A., Kang,H., Kim,H., Kwag,E., Lee,J.Y., Koo,O.,
            Park,J., Kim,H.K., Jo,D.G., Hwang,I., Ahn,J.Y., Lee,Y., Choi,J.Y.,
            Lee,Y.S. and Shin,J.H.
  TITLE     Loss of zinc-finger protein 212 leads to Purkinje cell death and
            locomotive abnormalities with phospholipase D3 downregulation
  JOURNAL   Sci Rep 11 (1), 22745 (2021)
   PUBMED   34815492
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10952)
  AUTHORS   Bao,C., Bajrami,B., Marcotte,D.J., Chodaparambil,J.V., Kerns,H.M.,
            Henderson,J., Wei,R., Gao,B. and Dillon,G.M.
  TITLE     Mechanisms of Regulation and Diverse Activities of Tau-Tubulin
            Kinase (TTBK) Isoforms
  JOURNAL   Cell Mol Neurobiol 41 (4), 669-685 (2021)
   PUBMED   32424773
  REMARK    GeneRIF: Mechanisms of Regulation and Diverse Activities of
            Tau-Tubulin Kinase (TTBK) Isoforms.
REFERENCE   5  (bases 1 to 10952)
  AUTHORS   Bowie,E. and Goetz,S.C.
  TITLE     TTBK2 and primary cilia are essential for the connectivity and
            survival of cerebellar Purkinje neurons
  JOURNAL   Elife 9, e51166 (2020)
   PUBMED   31934864
  REMARK    GeneRIF: TTBK2 and primary cilia are essential for the connectivity
            and survival of cerebellar Purkinje neurons.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10952)
  AUTHORS   Evangelista,M., Lim,T.Y., Lee,J., Parker,L., Ashique,A.,
            Peterson,A.S., Ye,W., Davis,D.P. and de Sauvage,F.J.
  TITLE     Kinome siRNA screen identifies regulators of ciliogenesis and
            hedgehog signal transduction
  JOURNAL   Sci Signal 1 (39), ra7 (2008)
   PUBMED   18827223
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10952)
  AUTHORS   Sato,S., Cerny,R.L., Buescher,J.L. and Ikezu,T.
  TITLE     Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase
            candidate, is involved in tau phosphorylation and aggregation
  JOURNAL   J Neurochem 98 (5), 1573-1584 (2006)
   PUBMED   16923168
REFERENCE   8  (bases 1 to 10952)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   9  (bases 1 to 10952)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 10952)
  AUTHORS   Tomizawa,K., Omori,A., Ohtake,A., Sato,K. and Takahashi,M.
  TITLE     Tau-tubulin kinase phosphorylates tau at Ser-208 and Ser-210, sites
            found in paired helical filament-tau
  JOURNAL   FEBS Lett 492 (3), 221-227 (2001)
   PUBMED   11257498
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL935168.11.
            
            On Dec 23, 2022 this sequence version replaced NM_080788.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077011.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               AL935168.11        139058-139220       c
            164-237             AL935168.11        113832-113905       c
            238-373             AL935168.11        111063-111198       c
            374-521             AL935168.11        95349-95496         c
            522-595             AL935168.11        78872-78945         c
            596-736             AL935168.11        74569-74709         c
            737-841             AL935168.11        72285-72389         c
            842-907             AL935168.11        66124-66189         c
            908-1000            AL935168.11        62410-62502         c
            1001-1126           AL935168.11        61819-61944         c
            1127-1284           AL935168.11        48787-48944         c
            1285-1501           AL935168.11        44420-44636         c
            1502-1713           AL935168.11        38828-39039         c
            1714-2302           AL935168.11        37025-37613         c
            2303-3573           AL935168.11        33609-34879         c
            3574-10952          AL935168.11        21406-28784         c
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..10952
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /note="tau tubulin kinase 2"
                     /db_xref="GeneID:140810"
                     /db_xref="MGI:MGI:2155779"
     exon            1..163
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..4036
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein bartleby"
                     /codon_start=1
                     /product="tau-tubulin kinase 2 isoform 1"
                     /protein_id="NP_542966.2"
                     /db_xref="CCDS:CCDS16626.1"
                     /db_xref="GeneID:140810"
                     /db_xref="MGI:MGI:2155779"
                     /translation="MGLANRRHRLGTCSPISTRGGENCKTGKIKCLLKETHSWLRREI
                     GNRLNGIHPWELDACVAVLPHQGFAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFG
                     EIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNY
                     VVMQLQGRNLADLRRSQSRGTFTISTTLRLGKQILESIESIHSVGFLHRDIKPSNFAM
                     GRFPSTCRKCFMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHD
                     DLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSTFLDHISSL
                     DYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKSGTDGSLTTTTTSATPQLHTRLTP
                     AAIGIANATPIPGDLLRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKD
                     VWEEMDINKNKIKLGICKAATEEENSHGQVNGILNAPSLGSPIRVRSEITQPDRDVPL
                     VRKLRSIHSFELEKRLTLEPKPDTDKFLETCMEKMQKDSSAGKEPVPPALPHKPCVPV
                     VTHTDHIWHYDDEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEVDSKEWVIV
                     DKEQDLQDFRTNEVLGHKTTGSPSDEEPEVLQVLEGSPQDEKIQVGPWTDNHHLKKES
                     SGVVLALSAECPATAASELYTDRLDLQAGAASQFITVTPTSPMEAQAEGPLTAITIPR
                     PSVASTQSTSGSFHYGPQPEKKDLQPLEPTVELYSPRENFSGLVVTEGELASGGSRVD
                     LGLQIDHTGHDMLPNMRDGDTSQDLGPKDPPDHNRLAVKEFEHLPGETEERSLLLGSE
                     NEDERLSKGQHCIEVSSPGELVTAERAQLAATEPLHVSETQNCSVLPNQDKTHEIMKL
                     LAVGTSEISPQAIDPHAEGQIGQMAAMQKNKLFKDDGIQSESLPRQQGDLSAFLHQEG
                     KREKVVPRNGELYHCVSENEHGPPTRKDMLRSSFVTRHSRIPVLAQEIDSTFESSSAI
                     SAKEKLLQKKAYQPEIVKLLVEKRQFKSFLGDLSSASDKLIEEKLAAVPVPFSEEEVF
                     APFSRLAADSHLSRSVEDSFLSPIISQARKSKIPRPVSWVSTDQINGSASPQFLPRPP
                     PGKPPVRPGVEARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKSRSTTQCKSPGSPHN
                     PKTPPKSPVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSH
                     SGSSSSRRSCQQEHCKPSKNGPKGSGSLHHHSTSSKTPPGKSKPASKLSR"
     exon            164..237
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            238..373
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            374..521
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            522..595
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            596..736
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            737..841
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            842..907
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1000
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1126
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1284
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1501
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1713
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..2302
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..3573
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..10952
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10927..10932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /note="hexamer: AATAAA"
     polyA_site      10952
                     /gene="Ttbk2"
                     /gene_synonym="2610507N02Rik; B930008N24Rik; mKIAA0847;
                     Ttbk; Ttbk1; TTK"
                     /note="major polyA site"
ORIGIN      
        1 aactcgccct ctcaggcaaa cctgaagata gcggcaattc tccggtgggg ggcggagaca
       61 ctctcggccc aattctccgc ccccttcctg ataggcaatg ggactagcca accgacgcca
      121 caggctggga acgtgctctc caatcagcac ccgcggcgga gagaattgta aaaccggtaa
      181 gatcaagtgc ctattgaaag agactcactc ttggctaaga agagaaatag gaaataggtt
      241 aaatggaatc cacccttggg agctagatgc ctgtgtagct gttttacctc atcagggttt
      301 tgcaatgagt ggaggaggag agcagccaga tatcctcagt gttggaatcc tggtcaaaga
      361 aagatggaaa gtgttaagaa agattggagg tgggggcttt ggagaaattt acgatgcctt
      421 ggacatgctc accagggaga atgtggcgct gaaggtggag tcagctcagc agccaaagca
      481 ggttctgaag atggaggttg ctgtgttgaa gaaactgcaa gggaaagacc atgtttgtag
      541 atttattggc tgtgggagaa atgatcgttt caactacgtg gtcatgcaat tgcagggacg
      601 gaatctggca gatctccgcc gtagccaatc ccggggcaca ttcactatta gcactaccct
      661 tcgtcttggg aaacagattc tggagtctat tgaaagcata cattctgtgg gattccttca
      721 cagagacatc aaaccgtcaa acttcgccat gggacgtttc cccagtacat gtaggaaatg
      781 tttcatgctt gattttggct tggctcgaca atttactaat tcctgtggtg acgtcagacc
      841 acctcgtgct gtggcaggct ttcgagggac agttcgttat gcatcaatca atgctcatcg
      901 gaacagggaa atgggaagac atgatgacct ttggtcttta ttctacatgt tggtggagtt
      961 tgtggttggc caactgcctt ggagaaaaat aaaggacaag gagcaagtag gctccattaa
     1021 ggagagatat gaccacaggc tcatgttaaa acacctccct ccagaattca gcacctttct
     1081 tgaccatatt tcctctttgg attattttac aaaaccggac taccagcttc taacatccgt
     1141 gtttgacaat agcatcaaga cctttggagt aattgagagt gacccgtttg actgggagaa
     1201 gagtggaact gatggctccc tgacaaccac caccacctct gccacccctc agctgcacac
     1261 ccgcctgacc cctgctgcta tcggaattgc aaatgccacc cccatcccag gagacttgct
     1321 tcgagaaaat acagatgaag tgtttccaga tgaacagctt agtgatgggg agaacggaat
     1381 ccctgttggt gtatcaccag ataaattgcc tggatctctg gggcacccac gccctcagga
     1441 aaaggatgtc tgggaagaga tggatatcaa caagaacaag ataaagctgg gaatttgcaa
     1501 agcagctact gaagaagaaa atagccatgg tcaagtaaat ggcatactca atgctccaag
     1561 ccttggttca ccaattcgtg tccgatcaga gattactcag ccagacagag atgttccgtt
     1621 agtaaggaag ctacgttcta ttcacagctt tgagctggaa aaacgtttga cacttgagcc
     1681 aaagccagat actgacaagt ttctggagac ctgcatggag aaaatgcaga aagattccag
     1741 tgcaggaaaa gaacctgtcc cccctgctct gcctcataag ccgtgtgtcc ccgtcgtgac
     1801 ccacactgac cacatctggc actatgatga cgaatatctt cctgatgcct ccaagcctgc
     1861 ctctgccaac accccggagc aggcagatgg tggtggcagc aacggattta tagctgttaa
     1921 cttaagctct tgcaaacagg aggttgattc caaagaatgg gtgattgtgg acaaggagca
     1981 agaccttcag gactttagga caaatgaggt gttaggccat aagacaactg gaagcccttc
     2041 agatgaggag cctgaagtgc ttcaggtcct tgagggatca cctcaagatg aaaagatcca
     2101 agtaggtcct tggactgaca accatcactt aaagaaggaa agctcaggtg tggttttagc
     2161 actttctgct gaatgccctg ctactgctgc ttcagaactg tacacagata ggctagacct
     2221 ccaggctgga gctgcaagtc agttcatcac agtgactccc acaagtccca tggaggcaca
     2281 agcagaagga cccctgactg cgattacaat tcctagacct tctgtggcat caacacagtc
     2341 aacttcagga agctttcact atggcccaca accagagaag aaagatcttc agcccttgga
     2401 gcccactgta gaactgtact ctccaaggga gaacttctcc ggcttggttg tgacagaggg
     2461 tgaactagct agcggaggaa gcagagtgga tttgggactt cagatagatc acactggtca
     2521 tgacatgtta cccaacatga gagatggtga cacatctcaa gacttgggac caaaagaccc
     2581 tcctgaccat aatagattag ctgtgaaaga atttgaacat ctccctggag aaacagaaga
     2641 gagaagcctt cttctgggct cagagaatga agatgagagg ttaagtaaag ggcagcactg
     2701 tattgaagtc tcttccccag gagagttagt gactgcagag agggctcagt tagctgccac
     2761 agaacctctg catgtatcag agacacagaa ctgtagtgtg ctaccaaatc aggacaaaac
     2821 ccatgagata atgaagcttt tggcagttgg aacttcagaa atttctcctc aagccattga
     2881 cccacatgct gaagggcaga taggccagat ggcagcaatg cagaaaaata agctatttaa
     2941 agatgatggt attcagagtg aaagcttgcc aaggcagcag ggagacctct ctgctttttt
     3001 gcaccaagag ggtaagagag agaaagttgt ccctagaaat ggagagctct atcattgtgt
     3061 ctcagagaat gagcatggtc ctcctactcg gaaggacatg ctccggtcat cctttgtgac
     3121 caggcacagc cggatccctg ttttagcaca agaaatagac tcaacttttg aatcatcctc
     3181 tgctatttct gcaaaagaaa agcttctaca gaagaaagcc tatcagccag aaatagtcaa
     3241 acttcttgta gaaaaaaggc agttcaagtc tttcctggga gacctctcaa gtgcctctga
     3301 taagctgata gaggagaaac tagccgctgt tccagtccct ttttctgagg aggaagtctt
     3361 cgctcccttt tctagactgg cagcagattc ccacctgagt agatcagttg aagatagctt
     3421 tctgtcaccc atcatctccc aggctagaaa gagcaagatt ccaaggccag tatcctgggt
     3481 cagcacagat caaattaatg gctccgcttc acctcagttc ttgcctcggc caccaccagg
     3541 aaagccacca gtcaggcctg gagtagaagc caggctacgc agatataaag ttctagggag
     3601 tagtaactct gactcagacc ttttctctcg cctggcccaa attcttcaaa atggatctca
     3661 gaaatcccgg agtactaccc aatgcaagag cccaggatct cctcacaatc caaaaacacc
     3721 acccaagagt ccagttgtac ctcgaaggag tcccagtgcc tctcctcgaa gctcatcctt
     3781 gcctcgaaca tctagttcct caccatctag ggctggacgg ccccaccatg accagaggag
     3841 ttcttcccca catctgggga gaagcaagtc accccctagc cactcaggat catcgtcctc
     3901 caggaggtcc tgccaacagg agcattgcaa acccagcaag aatggcccaa aaggatctgg
     3961 cagcctccac caccactcaa ccagctctaa aactccccca gggaagagta agccagccag
     4021 taaactcagc agataggaag tgagcaggct acatctctgg gaaaggcgtg agatcttctt
     4081 cctcctagac ctgatgcatg tgtgtccctg tactgtctgt ttcaaacaat cagtgttgat
     4141 cttctcttgc aaaagaaagt aacatgatca gttctttata agaagaccta agtataacta
     4201 gctagggcag gtagaagcac atcaggagtc cgtgcctctg cacaggaagc gtcagtccag
     4261 cagtgtccga ggggaagaaa ctcaggcctg agctcttgtt tatagctgcc tttgaaacag
     4321 aaaattgagg ataggaaata gaatgggatt ttaggtgatt ttgcctaatt tgttaatctt
     4381 ctactcaaaa agtatatagc aaaattgttt gatattttta tttaaaactt cctaaccttg
     4441 gagagtcatt gcgcagacat tcaaatataa gaagcttgtt gccaggaaac cattacctgg
     4501 gtatatttgg tttgtgtgtt tatttagtga atctacacct ttctttccac tcattcctag
     4561 tatcttccct gaattttaca ttttttaaaa ttaatgtttt tacctcttat ttttaacaat
     4621 acaccctaat gtgtgttttc atcagcatag gataatccaa ttacctttat ttagtcaaac
     4681 cttgctattt tcgtttgagc aacagttctt attcttaact cctatatttt tatctcaaaa
     4741 atgaaagaaa tgtctatcta gagctatttt gggcaaaagt atctggagag ggacagagtg
     4801 ggacaatggc tttcaaaggt acaagcacct ttcccctgac tgcacttcct cctctcagga
     4861 gcctgcggag gagacagctt ccctgggttc tttctagtgt cagccttcag ggtgatctag
     4921 gaaagaataa cacttcggaa tgtggcttat acttttagag atttagaaag aaaatatgtt
     4981 actaaggctt acaatcagat tttgtatttt atacatttag ccaataagga atgaatatct
     5041 gggaaataaa tttagactaa atagttttct ttcaatgttt ttattacctg gttcttgtgt
     5101 tgtttaatgc ttgggcttct tagcctgact tccacattta taagaagacg taagtataac
     5161 tagctagggc aggtaggaag cacatgagga gtccgtgcct ctgcacagga agcgtcagtc
     5221 cagcacagtc caaggggaag aaactcaggc ctgaattctt gtttatagct gctgttgaca
     5281 tctaaatttc agtttataaa gctgtaagga tgatcatatt cgttctaatc ttactctctt
     5341 actagcagaa atcaggcaaa ttaaagacac cagacttttt aagtggacta tttgatgctc
     5401 tgtgggtgta taaaaatctt attgaggtca gggaaattga tctgcctgct gttgaaatct
     5461 gccttctaac aaacatctac tttttgactt tgtattgctg ccaaacctgt aactgttgaa
     5521 tttcaggagt ggggtggaaa aggaaacgtg tttcttcact aagtgagcat gttccagggg
     5581 tgcatcccag ctgcccaggc actaaactaa cttagcttgt tctgtgttga gcgttttcaa
     5641 acttgggaca ctacactgag acataaggtc tcccatgaga aatggggcat agtgtggaat
     5701 cttaatttct gtccaggttc aggcacttga gagcagtgca gtaggtaagg cgcatttgtt
     5761 gtttatctat tacagctgta atactggaga ctgatcctta tgtattatat tgtataacag
     5821 ttactgcagt actgcttgca tgtctttcat ggtaaattat ataaatattt ataaatatag
     5881 aagagacata tgcttatata aatccatttt tttctcattc tgcatttgta atttcaagat
     5941 tatagatgat aaaattcctt ttctattgtg tgcttcatgt acacttgggc attgcctgtc
     6001 ttcattttct gaacacatgt tcttggcatg tggtccctaa gagtagtcct gccttccggg
     6061 tgcacactga agcagctggc acttgttacc aagccaaggt gtaaggtact taattttcac
     6121 ttaaaaagta ttttaatagg ggaaaatttt aacaagatat gcttgtaata caggctcaaa
     6181 gattactcat gttagccaca tagttctgtc catatggtta gagtggatag atgggcatta
     6241 taattatgca gctgacatca actgtttttt aaaacatttc ctgaaagttg cctcttccat
     6301 gtcacttttt tgaagggaag tggggatggg atgtagggta actaaaattc tttctgttac
     6361 attagctaaa tgatgctaaa agtagccggc tttcttgctg tcctctctag tcaaacatgt
     6421 gctaatagta actgggaaag ctaacagggc ccagcctttt tctttgtgaa tttctctgct
     6481 ggcagtcatg ctgacagaga tgctttctgt ctgtctttca gtgtgttgcc tccttttctg
     6541 catgccgttc acaaactcat caaaatccag agacaaaacg cctcattatg tgagaatgtt
     6601 ttattttgta tgtagggaaa agaaccaacc aaagatgttt ctgctctgaa gaaaaggaaa
     6661 tggactaatt aattatatcg tatgtatcag tagttacatt tattattaag tgctgtcctg
     6721 atacagagat gaaaaagtag aacctggtgt gatggcacac actttgatcc tagcactctg
     6781 gaggaagggg caagtggatt tctgagtttg aggccagcct ggtctacaga gtgagttcca
     6841 gcacagctag ggccacacag agaaaccctg tcttaaaaaa acaaacaaaa gattaaaaaa
     6901 catagtttgt gcttttacat gttacatttc tttcaggtta aaaataaaaa ttggcagtct
     6961 taagccagct cttcactcag gagtatgatc tagagaatca agctttcggt ttggtttggg
     7021 ttttcagttc tttgaggttt ttattgatgg catatttcat atttcagtca caaatcatcc
     7081 aaataaggta aagaacatga ctgtagccat aatagaagta gggtgtgaca gaaattatct
     7141 tttgggagac aagtacctcc tggcttctat ctacacaggt tactaactca ggcctgtcct
     7201 gtgcttattt cagtggagca aagaatgaac acttagttcc taattttagg taatgttgcc
     7261 cccaaattat actactgacc cgtaggcttt gtgaattgct tgaaatatta aaatcatacc
     7321 tcgtaatctt gcacatccta aagatatctt gactttaatg gatagtgaaa acaggaggga
     7381 attgtatcct atctgattca gcattaagat agagatctta aaacatctag gcaagctggg
     7441 cgtggtggcg cacgccttta atcccagcac tctagaggca gaggcaggtg gatttctgag
     7501 ttagaggcca gcctggtcta caaagtgagt tccaggacag ccagggttat acagagaaac
     7561 cctggctaca aaaacaaaaa cacaaacaaa caaaacacca aaaaacaaaa aaaatctagg
     7621 ctcttctctc cagctctgca cacaggctgg ccttgcaaac aggagcacag ccacttttag
     7681 ttctttagtt gcatgctttg caatgaatat ctttggtaaa agttgcaaca agttaatcaa
     7741 ggcctaaaaa aaaaagtaac aaacggtcaa agagtccttg acgtcattat ccactgcaca
     7801 ttatttgctc cagcccactg aagcactgta tgctgatgcg gtgtttcatt ttactcctgg
     7861 acactgcggg aacttgaggg gctgcagcaa gtaccagact ccctctcagt cacagagtct
     7921 taggcttctg tttctgtgat tgcatccatt gtacaaaagc aaaggtgtac attgctgcat
     7981 gttttataaa cctagactgc atagctagct aagtaatgag attttctaca cccaataata
     8041 accttgacaa acaggtgcct tttcctcaag ggcagaaaat tttacatttc taatctaaca
     8101 ttaggaattg gtgataggag aaacaggatc cacaatttag tggctttagg aaaaagatgc
     8161 atcttgaaaa tgttatctta aaatatactt atagataatc tagatttagc cttggagatc
     8221 aaattttctc atattttaaa acttgttagc taggtgtgat ggctaatgtt tctaaccaat
     8281 acttgaggga ctgaggtgga aagatggaag agagtctgaa gccaacctct gcttcatagt
     8341 gattgcaagt ccacctgggc tgtagtgtgg gatcctctcc caaaaaacaa aaacaaaaac
     8401 attatttttt taattatttt tttaagtttt catacatgta tgtatatatt atatgttgag
     8461 tacacagctc tcatttgtgt ataattactt ctgtcatgtt catggtttgc attcactgtt
     8521 ttacattgtg cctatttatg gctttggaat gttgtttctg gggggaggtc atctagcacc
     8581 tctgtcactg acgtaatagg acatttgtaa ccaacacctc tgtcactgag gtaataggac
     8641 atttgtaacc aactgtactg cttaaatttc ttgaatttgg ttctatagac aacatatttg
     8701 cagaatagct ttttatgttt taatccaaat gcattataag attacagcat gtgacgactc
     8761 tgatggtcct tacatgccaa caacacttag agtcccaact cagaagccac agtgatgcat
     8821 attagacctt aactcataga agaaaactag aggcagttcc acaacccagt cagtgggagg
     8881 tgtctggtaa acatgcccca cttcctatcc tcaccaaaac tttacaaaac tcgagtgtgc
     8941 actctgagaa tgtgtaagta aagtagaaag tcctcatatt attgggtatt tgccttattt
     9001 tggccatttc tccagaaatt ttacacaatc ttggagcctt aaggatcttg ttatgcagca
     9061 ttgctttcca taactgcagg aagtgggaaa ttcctaggta actcccaagt atggaacaaa
     9121 aagaacccct attcacttag aggaaaatat gggtaaaata tatatgtttt caggtaagtt
     9181 cttcctcttg ggagaggatt tgcatcagaa aattagtgac aaattcatct cttggtttca
     9241 caatatctca agatgctaaa tagcaaagat ttactgagtc atgatttcag tatttgctcc
     9301 tatataagta ctaataaaca tgacagctaa ggcaagttca aggtgatgag atgatttcct
     9361 atactgggct caatccttgg ttttaaggag cccttaacaa aagtcctttc attgccctta
     9421 aaatgtaaca agtttctgcc ttctctaatt ggaggtgata gtatctacac tgtaggctca
     9481 aggtgatgct gaccacatct cctggtacct tcaactagaa tttctagacc aacaacagta
     9541 tgaatgacgc agatctctga gactaataga attacagaat ttgctaataa cgttatttta
     9601 cattaacagt gggatttatc tcttgataat ttaaaattat tttgtactgt atacatgatt
     9661 attcacctaa accaagtttt tatgtagact attcttgcca tatgatactt tttaaaactc
     9721 ccataaaaca taattgaaaa catcaaaaat tcacttgtat caaagttgga atgtgccagg
     9781 tgttttttgc tcacttacag tagtaaacaa ggctatacag agtttgggct gtgatttatt
     9841 attttaggga gtttgctgag aagaaacctt aagactttat tcaaaacagt ttggtttggt
     9901 ttgatttttt gagatggggg ttttttcagt gtagccctga gctggaactt gctctgtaga
     9961 ttaggctggc cttaaactcc agacctacct acctctgcct ccagagtgct gagataaaaa
    10021 ggtgtgagcc accatgcctg gctaaaaaca gttttattaa actataaatc aatgaaatta
    10081 gttctctaag ataattaaga aattaaaata taggaaatag atcttctttt taagacaaag
    10141 gaaatgtagc actgggccac tcaggacttt gaaattggat tggatttcct agattttggt
    10201 ggtatatgcc tataatctca acattgaaga ggtaagggca ggaacaggag ttgaaggtca
    10261 tacataatga gctgaataat gagttcgaag ctaacttagg ctctatgaga ccctgtctca
    10321 aaataggggg gaggggaaag gcagaaacaa aatcttaggt aagagagatt ggattgttgg
    10381 attggatgag tagacagatc tgatggcctg ggctcgagcc actttgctgt tccctgatgc
    10441 acagttctca ttctcctttg acacctttcc taaatggcat ctatttaatc agatcattta
    10501 tttacagatt ccaagttttt cagaaaagga ggcacatctc attttgtatt agatataatg
    10561 agttcataaa ttattactgg caaatgttat caaacttagt cataacccga attctcttac
    10621 tgaacaagag aaataagtca atgttctgct gtcctcctct taatgagatg cgaaattgct
    10681 ctttgaagtt cttttcaatg tgttacattg tatcctttct ccttcaagca tcttcccttt
    10741 atgtgttttt gctcaggcaa ccatgggcat agagtattga aaaacatgca gtgcttttgt
    10801 ataggccatc tgtacataaa agtgtaatta tttatttaat tttctcattt gtaccatatt
    10861 aaagctttgt acagtgtttt aagttctgtt ttaaaattat tttgtatttt atttttataa
    10921 tctagtaata aaacattcag tccgcatgca aa
//
