LOCUS       NM_001162864            8734 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tau tubulin kinase 1 (Ttbk1), mRNA.
ACCESSION   NM_001162864 XM_001476670 XM_001480065
VERSION     NM_001162864.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Bao,C., Bajrami,B., Marcotte,D.J., Chodaparambil,J.V., Kerns,H.M.,
            Henderson,J., Wei,R., Gao,B. and Dillon,G.M.
  TITLE     Mechanisms of Regulation and Diverse Activities of Tau-Tubulin
            Kinase (TTBK) Isoforms
  JOURNAL   Cell Mol Neurobiol 41 (4), 669-685 (2021)
   PUBMED   32424773
  REMARK    GeneRIF: Mechanisms of Regulation and Diverse Activities of
            Tau-Tubulin Kinase (TTBK) Isoforms.
REFERENCE   3  (bases 1 to 8734)
  AUTHORS   Yan,Y., Yan,H., Teng,Y., Wang,Q., Yang,P., Zhang,L., Cheng,H. and
            Fu,S.
  TITLE     Long non-coding RNA 00507/miRNA-181c-5p/TTBK1/MAPT axis regulates
            tau hyperphosphorylation in Alzheimer's disease
  JOURNAL   J Gene Med 22 (12), e3268 (2020)
   PUBMED   32891070
  REMARK    GeneRIF: Long non-coding RNA 00507/miRNA-181c-5p/TTBK1/MAPT axis
            regulates tau hyperphosphorylation in Alzheimer's disease.
REFERENCE   4  (bases 1 to 8734)
  AUTHORS   Dillon,G.M., Henderson,J.L., Bao,C., Joyce,J.A., Calhoun,M.,
            Amaral,B., King,K.W., Bajrami,B. and Rabah,D.
  TITLE     Acute inhibition of the CNS-specific kinase TTBK1 significantly
            lowers tau phosphorylation at several disease relevant sites
  JOURNAL   PLoS One 15 (4), e0228771 (2020)
   PUBMED   32255788
  REMARK    GeneRIF: the acute effects of TTBK1 inhibition on tau
            phosphorylation in vivo, is reported.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8734)
  AUTHORS   Ikezu,S., Ingraham Dixie,K.L., Koro,L., Watanabe,T., Kaibuchi,K.
            and Ikezu,T.
  TITLE     Tau-tubulin kinase 1 and amyloid-beta peptide induce
            phosphorylation of collapsin response mediator protein-2 and
            enhance neurite degeneration in Alzheimer disease mouse models
  JOURNAL   Acta Neuropathol Commun 8 (1), 12 (2020)
   PUBMED   32019603
  REMARK    GeneRIF: Tau-tubulin kinase 1 and amyloid-beta peptide induce
            phosphorylation of collapsin response mediator protein-2 and
            enhance neurite degeneration in Alzheimer disease mouse models.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8734)
  AUTHORS   Asai,H., Ikezu,S., Woodbury,M.E., Yonemoto,G.M., Cui,L. and
            Ikezu,T.
  TITLE     Accelerated neurodegeneration and neuroinflammation in transgenic
            mice expressing P301L tau mutant and tau-tubulin kinase 1
  JOURNAL   Am J Pathol 184 (3), 808-818 (2014)
   PUBMED   24418258
  REMARK    GeneRIF: TTBK1 accelerates motor neuron neurodegeneration by
            recruiting proinflammatory monocytes and enhancing sensitivity to
            neurotoxicity in inflammatory conditions.
REFERENCE   7  (bases 1 to 8734)
  AUTHORS   Xu,J., Sato,S., Okuyama,S., Swan,R.J., Jacobsen,M.T., Strunk,E. and
            Ikezu,T.
  TITLE     Tau-tubulin kinase 1 enhances prefibrillar tau aggregation and
            motor neuron degeneration in P301L FTDP-17 tau-mutant mice
  JOURNAL   FASEB J 24 (8), 2904-2915 (2010)
   PUBMED   20354135
  REMARK    GeneRIF: TTBK1 up-regulation enhances tau phosphorylation and
            oligomerization, whose toxicity results in enhanced
            neurodegeneration and locomotor dysfunction in a tauopathy animal
            model.
REFERENCE   8  (bases 1 to 8734)
  AUTHORS   Sato,S., Cerny,R.L., Buescher,J.L. and Ikezu,T.
  TITLE     Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase
            candidate, is involved in tau phosphorylation and aggregation
  JOURNAL   J Neurochem 98 (5), 1573-1584 (2006)
   PUBMED   16923168
REFERENCE   9  (bases 1 to 8734)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151275.3.
            
            On Oct 18, 2022 this sequence version replaced NM_001162864.1.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164141
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               AC151275.3         23253-23480
            229-390             AC151275.3         26595-26756
            391-538             AC151275.3         30658-30805
            539-612             AC151275.3         31645-31718
            613-753             AC151275.3         31901-32041
            754-858             AC151275.3         33041-33145
            859-924             AC151275.3         33393-33458
            925-1017            AC151275.3         33894-33986
            1018-1143           AC151275.3         34067-34192
            1144-1298           AC151275.3         37230-37384
            1299-1530           AC151275.3         40097-40328
            1531-1709           AC151275.3         40509-40687
            1710-2271           AC151275.3         43219-43780
            2272-3827           AC151275.3         63189-64744
            3828-8734           AC151275.3         65349-70255
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.9 cM"
     gene            1..8734
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /note="tau tubulin kinase 1"
                     /db_xref="GeneID:106763"
                     /db_xref="MGI:MGI:2147036"
     exon            1..228
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            229..390
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..4209
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="tau-tubulin kinase 1"
                     /protein_id="NP_001156336.1"
                     /db_xref="CCDS:CCDS50125.1"
                     /db_xref="GeneID:106763"
                     /db_xref="MGI:MGI:2147036"
                     /translation="MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGG
                     FGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKF
                     NYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNF
                     AMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKNREMGR
                     HDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIA
                     SLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQT
                     AAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPPEGLGPGPHLVPHP
                     GGPEAEVWEETDVNRNKLRINIGKTPCVEEEQSRGVGVPSSPVRAPPDSPTTPVRSLC
                     YRRVNSPESERLSTAADGRVELQERRSRMDLPGSPSRQACSSQPAQMLSVDTGHADRQ
                     ASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGAEPSTSG
                     TTDEEPEELRPLPEEGEERRRLGTEPTVRPRGRGMHTLTEEDPRQMLPQPAPPQLSQA
                     DGRSETSQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPL
                     ALPYQDFKRDLSDYRERARLLNRVRRVGFSHMLLTAPQVPLAPFQPQANGKEGEEEEE
                     EEEEEEEEEEEEEEEEEEEEEEEEEEEEAGALGEVLGPRSGSSSEGSERSTERSQEGA
                     PSTLLADDQKEARGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPDPDLGTL
                     AALTPQHERPQPTGSQLDVSEPGTLSSILKSEPKPSGPGAGGGVGLVAPGAGVTAVTS
                     PFTKVERTFVHIAEKSHLNVMSSGGQASRPEELSTGGELGLEVLSEGGIAEEGAPAPL
                     ENGMALAGLDGTEMESCALSGPPGETPSEVVTDSLPNGPALADGPAPASQQEPVTKKG
                     TTISPSRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVME
                     KRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPTTALPRKAVRAATTR
                     SRIPRPISVSMPVEGQQLPGRPHGAASATDLAITSRLQLQKPSGLAPAADLRPKQSAS
                     RGPGPGRAQVSKPAAPRSPGLPASTARHPSGSPRSQSLSRKESSSPSHQARPGVPPSR
                     GVLQVRSQPEASPVAPKKGPKGKQLQTQRAATKGRAVVSEGRPGAR"
     exon            391..538
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            539..612
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            613..753
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            754..858
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            859..924
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1017
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1143
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1298
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1530
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1709
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..2271
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..3827
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3828..8734
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6915..6920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      6937
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /note="major polyA site"
     regulatory      8698..8703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
                     /note="hexamer: AATAAA"
     polyA_site      8734
                     /gene="Ttbk1"
                     /gene_synonym="C330008L01Rik"
ORIGIN      
        1 acttcgcagc cgccgccgtc gcccgaggag gatcggggcc gggccgggcc gggccgggat
       61 ggtcgcggtc gggaggccgc cgccgccacc gccggaggga gcgggccgcc cagcgctgca
      121 ctgagccgcc cccgccccgc cccggccccg ggggatgcgc cgccccgcgc cgctgccttc
      181 accgccgccg ccgcagccgc agccgcagcc gcagcggaca ccgagcaggt agatggcccc
      241 ccaagggcag gccctgcggg cacccctccc tccggctggc ggatgcagtg cctagcggca
      301 gctcttaagg acgaaaccaa catgagtggg ggaggggagc aggccgacat cctgccggcc
      361 aactacgtgg tcaaagatcg ctggaaggtg ctgaaaaaga tcgggggcgg gggcttcggt
      421 gagatctatg aggccatgga cctgctgacc agggagaacg tggctctcaa ggtggagtca
      481 gcccaacagc ctaagcaggt cctcaagatg gaggtggctg tgctcaaaaa gcttcaaggc
      541 aaggaccacg tgtgcaggtt cattggctgt ggccgtaacg agaagtttaa ctatgtggtg
      601 atgcagctcc agggccggaa cctggctgac ctgcgccgca gccagccaag gggcactttc
      661 acgttgagta ccacactgcg cctgggcaag cagatcctgg agtccattga agccattcac
      721 tccgtgggct tcctgcaccg tgacatcaag ccgtccaact ttgccatggg ccggctgccc
      781 tcgacctaca ggaagtgcta catgttggac tttgggctag ccaggcagta cactaacacc
      841 actggggacg tccggcctcc tcggaatgtg gccgggttcc gggggactgt ccgctacgcc
      901 tcggtcaatg ctcacaagaa ccgggagatg ggccgccatg atgacctgtg gtccctcttc
      961 tacatgctgg tggagtttgc tgtgggccag ctgccctgga ggaagatcaa agacaaggag
     1021 caggtgggga tgatcaaaga gaaatacgag caccggatgc tactgaagca catgccatcc
     1081 gagtttcatc tcttcctgga ccacattgcc agcctcgact acttcaccaa gcccgattac
     1141 cagttgatca tgtcagtgtt tgagaacagc atgaaggagc ggggcattgc cgagaacgag
     1201 gcctttgact gggagaaggc aggcactgat gccctcctgt ccacgagcac ctccacccca
     1261 ccccagcaga acacgcgaca gaccgcagcc atgtttggag tggtcaatgt gacaccggtg
     1321 cctggggacc tgcttcgtga gaacactgag gacgtactgc agggagagca cctgagtgac
     1381 caggagaacg cacccccaat cctgcccggg aggcctccag agggcttggg ccctggtccc
     1441 catcttgttc cccaccctgg gggtcctgag gctgaagtgt gggaggagac agatgtcaac
     1501 cgcaacaaac tgcgcatcaa cattggcaaa accccctgtg tggaggaaga gcagagtcga
     1561 ggagtagggg tccccagttc cccagtgcgt gcccccccag actccccaac aaccccagtg
     1621 cggtctctat gctaccggag ggtcaacagc ccagagtcgg agaggctgtc tacagctgct
     1681 gatggtcggg tagagctgca ggagaggagg tcacggatgg atctgcctgg ctccccctcc
     1741 cgccaggcct gctcctcaca accagctcag atgctgtcag tggacacggg ccatgctgac
     1801 aggcaggcca gtggccgcat ggacgtctct gcctctgtgg agcaggaggc cctgagcaac
     1861 gccttccgct cagtgccact ggctgaggag gaagactttg acagcaagga gtgggtcatt
     1921 attgacaagg agacggagct caaggacttc cctcccgggg ctgagcctag cacatcaggc
     1981 accactgatg aagagcccga ggagctgcgg cccctgcccg aggagggcga ggaaaggagg
     2041 cgactgggga cagagcccac tgtcaggccg cgtggacgtg gtatgcacac gctgacagag
     2101 gaggacccac ggcagatgct gccccagcct gcaccacctc agttgagcca ggctgatggc
     2161 cggtcagaga catcacagcc acccacgcct ggcagccctt cccactctcc cttgcactcg
     2221 ggaccccgac ctcgacggag agagtcagat cccacgggcc ctcagagaca ggtgttctct
     2281 gtggcgcccc cgttcgaggt gaatggcctt ccacgagctg tgcctctagc cttgccctac
     2341 caggacttca agagagacct ctctgattat cgagaacggg cccggctgct caacagggtc
     2401 cggagggtgg gcttctcaca catgctactc accgcccctc aggtcccatt ggctcccttt
     2461 cagcctcagg ccaatgggaa agagggagaa gaggaagagg aggaggaaga ggaggaggag
     2521 gaagaagaag aagaagaaga ggaagaagaa gaggaggaag aggaggaaga ggaagaggag
     2581 gaggaggcag gggcccttgg ggaggtgctg gggcctcgaa gtggctcaag cagtgagggt
     2641 agcgagcgga gcaccgagcg gagccaggag ggcgccccgt ccacactgct ggctgacgac
     2701 cagaaagagg ccaggggccg ggcctccatg gctgatgggg acctggagcc tgaggagggc
     2761 tccaaaacgc tggtgctcgt ctcccctggt gacatgaaga agtcaccagt cactgccgaa
     2821 ctggcccccg accctgatct gggcaccttg gctgcgctca cccctcagca cgagcggcct
     2881 cagcccactg gaagccagct ggacgtatca gagccaggca ccttgtcctc catcctcaag
     2941 tccgaaccca agccctcagg acctggagca gggggtgggg ttgggctggt ggcccctggg
     3001 gctggggtca cagcagtgac gtcaccattc accaaagtcg aaaggacttt tgttcacatt
     3061 gcagagaagt cccacctcaa cgtcatgtct tccgggggac aggcctccag gccagaggag
     3121 ttgagcactg ggggcgagtt gggcctggag gtgctctctg aggggggcat cgcagaggaa
     3181 ggggccccag cacctctgga gaatggcatg gctttggcag ggctggacgg gactgagatg
     3241 gaaagttgtg ctctgtcggg ccctcccgga gaaacgccct cagaagtagt cacagactcc
     3301 ttgcccaatg ggccagccct tgctgatgga ccagctccag catcccaaca agagccagta
     3361 accaagaaag ggaccaccat ctcccccagt cgccatgcta tgccaggatc tcgccccaga
     3421 agccgtattc ctgtcttgtt gtctgaagag gatacaggct cagagccttc gggctcactg
     3481 tcggccaaag agcggtggag caagagggct cggccacagc aggacctggc acggctggtg
     3541 atggagaaaa ggcagggtcg gctgttgctg cggctggcct caggagcctc atcctcctct
     3601 agtgaggagc agcgccgtgc ctctgagaca ctctcaggca ctggatctga ggaggacaca
     3661 cctgcctcag aacccacaac agccttgcct aggaaggctg tgcgggcagc gaccacccgg
     3721 agccggattc cccgtcccat cagtgtgtcc atgcctgtgg aaggccagca gcttcctgga
     3781 agaccccacg gtgcggcctc agcgaccgac ttggccatca ccagcaggct ccagctgcag
     3841 aagccctcag ggttggcccc tgctgctgac ctccgtccca aacagtccgc gagccggggt
     3901 cccggcccag gtcgcgccca agtctccaag cccgcagcac cccgcagtcc cggcctcccc
     3961 gcctccaccg cgcgccaccc cagcgggtcc ccgcggagcc agtccctgtc ccgtaaagag
     4021 agctcctccc cctcgcacca agcccggcct ggcgtccctc catcccgggg ggtcctccaa
     4081 gtcagatctc agccggaggc ctccccggtg gcccccaaga aaggacccaa ggggaaacaa
     4141 ctgcagactc agcgcgcagc aaccaaaggc cgggcggtgg tttcagaggg tcggcccggg
     4201 gccagataat gacacccacc cgcggctctc tgcggcaccc cctaccctca ccctggtccc
     4261 cacccgcagc cggccacact ggagccgctt ccagcacagc cttacgcgcc cgcgcaccac
     4321 cgcggcccca gcttcccgcc tgcacccacc aggacgcacg tgagcacatg ctgtgcccag
     4381 cagcccagcc agtccactga cccaggaggg tgcgtctttc tgtctgtctc tgcgtcctgc
     4441 ctctctcctg ccgcccctct tgcagggaca ggctcgcaac agatcctctg ccccgggaag
     4501 gctcagccac ctatcaactc tgaagactcc acttatggag cccatcccct tcgctcacca
     4561 ggtcttggct gatctctaac gccccccctc ccccgcccca accaaggggc ttacacctgc
     4621 ttacacctcc tgccatccac cttgttctct tcatccccac cttcttgtag ccagcctcca
     4681 aacctaccca acaggagaaa cagcctcaaa ttccataagt gcgcaggctc tggcccgccc
     4741 agtctcttag gagccatggt gaccatagaa ggccagtgtg ggatcgggga agtgggaccc
     4801 aggggtcatt ggtctattta gtcttaaggg gacaccacct ccggccttgc caaagtcagc
     4861 tctatgaaag aaaggaacag ggtgtatgat tctttgctcc ctggtgtctc tggggagcag
     4921 ataccccttt cctgaatagg aatgaatgga gcctctggga gcaagcacct gccctcccgg
     4981 gcaagcgttt cccagaggga atttggaatg gggctttaaa aagattactc acttctctgg
     5041 tcctctggtc tagtactcag gaccccttac catcaggaat tccttcctgc tgtctaacct
     5101 cactcctgcg tactgcagtt gccgccaact ctcttctctc aagttccaca caggaggaga
     5161 gtggttggcc atcatcttct gtgcgttggt catttggggg cttggctctc atccttcttc
     5221 ctgatgtctc tgctgtggga acaggctggc tcaggcatcc tcttcctgag tggacagagg
     5281 agacaggatg aggccgacca gctcctccgg gaggcagaag gctcttctca tgttagagtg
     5341 tgtgtgatgt agctggtgcc caccccaccc acccacaccc agttcaccct gggcccttct
     5401 tgctctgagc cagcccccac agctgagctg ctcggtacct gacatggggt cttctgctgc
     5461 attttggctt ctgcatagct tgtgcccgcc tcatcaggag ccccagctgc taacatctgg
     5521 agcaaacagc tgcagatggg acaggcaacc cctcctgtct gggacaggga ctccctgcag
     5581 gcctcctaga aagcactccc agccgcctcc gctcccctgt tctggtccct gtgtccttcc
     5641 ctgagccaca gagagcaaac taagacattc tggtgaaggc aggcctccgt atgccaccgg
     5701 cctggtcatg tctacacccg tgatgactgt ctgggagctg ctcatgccag ctgaactcct
     5761 cacatagggt cagcgggtgt gggcagtcgt ttccatgcca agagggcagc tctacagacc
     5821 agctggggag ggcaccaggg tggataggct ttgagatcta tcccacgctg acacagaagc
     5881 tcccagaaca ctcaggaaac ctctctggat gcagttatct ttgtcacccc aaggccctct
     5941 aattgtctcc tagtcccggg aaggggcaga gggctcactt cctcccaccc agagctttta
     6001 tgaatgacaa tcagctcatg ccaggtgggg accagggcat gatcccagcg cccagaccct
     6061 gggcctgccc cttgccacca acctaactgt gagtgtaggt gcccccctca gtctcccttc
     6121 tgccgtagga ccaacaacac ccaggtttgg agattgaaag aaggggaggc ctaggaaagc
     6181 cccgggctca ccccaccccc attacagcca atgcttgagt tggtacagat cagatgcgaa
     6241 ggccagtcta cccttgggtt ccaggcctct gagtcagtta tgtcactgga ttgtgaggaa
     6301 ggagggacag gggaccagca gagggggccg gtgagggggc taagtggttc ctcgccccag
     6361 gaaggaagca gggcagagga aaccacagct ccctcccgat gccccacgcc cgagctgctg
     6421 ccctttcctt gaggatcatc cgtcccctgt cctaggccct tcaggttccg ttccctcctc
     6481 tccgagagcg gcacagtacc actggctctt atgaccccca tcctgactgc ctctgtcact
     6541 ggctcagatg tggggactgc tcaccttaat gcgccttccc gccccagccg tactgtgtgc
     6601 agagggtgca ggagaagggt cctgccccag gcacatggct ctttggcgtc tcagctcctc
     6661 cccaggggac tggctcgctc agtcttctga gccccttggg caggtgttgg ggggcaggga
     6721 gttgccctcc ccctccctgg gaagcaaccg aagaatgttt acatgccaca cagaatgtaa
     6781 cagcccttgc ccaccccttc cagtcactgc atggcctctg cccaccctgt gcctgcccac
     6841 tcccctgaca ccaacacctt gcaagtcgct gtcacaacta gatcgggtct cagaagggaa
     6901 gtagccatca caccattaaa aagcctgtgg acctttctgt tggcctggac tctttttccc
     6961 ttcagggagg gcagggcaaa acccagcccc cgagggtggg ggtggggtga gcgggagaga
     7021 agatacagta tggtgtgagg tgggtggaga catcatgtcc gcagaggggg gaaagaaagt
     7081 tcagctccga ataggtgatt tggtcagggt ctgtcctagc agagcttccg cggagctcct
     7141 tgcttgtttc ggctgacggc tgtagtgtct ccattcggct gttcggctcg gcacagagag
     7201 agggaagaag caaagctggc atttccccga gggaagcaga gagagtcaag tgtttccagg
     7261 gaggcagaaa gcagagcagc ccacaggcgg tgccatagca aagcccatcc tgcccaccca
     7321 cctgtgccca ccctgcccac ccacctgtgg tatggcctct gcacctgcac aagtgtatcc
     7381 cctggataca cctgtgaagc accagagctt cgtctaccaa tctgaaacat cagaagtttg
     7441 gaaaccagca agcatctccc ttagctgggc acgctcgttc agggcatggt catttggtat
     7501 cagagcccga agggaaagcc aggcttgtcg gccttgtgcc tgtaatccta gcaaccacag
     7561 gacccaaacc agagggattc cagagagttc aaggccagcc tggactatgt aggaagttca
     7621 agaccagcct aggctaaatg ttagaccatc tcaaaaaaag aaaaaagaaa aagccagggg
     7681 aagctattta tattcactaa tatcacttat acattgaaaa aaaacttgag acaaggtctt
     7741 tccaggtagc ccaggctggc tgcaagtgtg ccatcctcct gcctcacact cccacgtgct
     7801 gggataacag agtgcactct gcttggttag atggagacag taatgtcttg gattatgaga
     7861 cactgctctt gacccctatt tataatatgt ggactatgca tggaatcact ttccttcctt
     7921 taaaaaaaaa aagtatttca tctctctatc ttctgtgatg cgaaatggtc ttggtaagag
     7981 gttgtagacc agaaagagaa aagattgctc aagagcaacc tcaactgcca ttggttgagg
     8041 ctgctcagca gagcaaggtc catacatgtg aggaggactc caccgtgggg tgctgtgaga
     8101 ctccatggac gaagtggccc ttctgtgttc ctataggcca gtctccacaa ctaagaagcc
     8161 acatgagggg gtacagcgag gcaggaggag gcttagtgtc tgtggcccac agggggagac
     8221 tgccttggaa agaaacagga agttcccttt gattttgaat cctaaactca ctagaagtga
     8281 atcttttcac ttagcggaca caaaaccagt tttcttcccc caggcctggc tccgggccac
     8341 acctggggtg ggaggtgaga ccacctgggt ttgaaagccc ctcccttcct gggggtgctg
     8401 cccttggata tccagaacct acgcttcctg ggaccccccc ccctctaact tgaacactgt
     8461 gggttcctgg ggccttgggt ggggcctgac gcaccatcac ttcatccccg tttaacatgt
     8521 cccgagcagg acatacttgt agtaaatgct aaaagcatct cagataaaag acaacccccg
     8581 tgcacgactc tgaccatcgg gagatgactt gggaatggct tggtatgtac cgtcacagac
     8641 cttttttatg tttataataa tgcctctctc tctctctgtc catatatatt tcttataaat
     8701 aaaagaggag catattatgc ctactgttca gaaa
//
