LOCUS       NM_032004               1358 bp    mRNA    linear   ROD 20-SEP-2024
DEFINITION  Mus musculus testis-specific serine kinase 6 (Tssk6), mRNA.
ACCESSION   NM_032004
VERSION     NM_032004.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Jha,K.N., Tripurani,S.K. and Johnson,G.R.
  TITLE     TSSK6 is required for gammaH2AX formation and the
            histone-to-protamine transition during spermiogenesis
  JOURNAL   J Cell Sci 130 (10), 1835-1844 (2017)
   PUBMED   28389581
  REMARK    GeneRIF: TSSK6 is required for gammaH2AX formation during
            spermiogenesis, and also link gammaH2AX to the histone-to-protamine
            transition and male fertility.
REFERENCE   3  (bases 1 to 1358)
  AUTHORS   Jha,K.N., Coleman,A.R., Wong,L., Salicioni,A.M., Howcroft,E. and
            Johnson,G.R.
  TITLE     Heat shock protein 90 functions to stabilize and activate the
            testis-specific serine/threonine kinases, a family of kinases
            essential for male fertility
  JOURNAL   J Biol Chem 288 (23), 16308-16320 (2013)
   PUBMED   23599433
  REMARK    GeneRIF: HSP90 function abolished the catalytic activities of TSSK4
            and -6 but did not significantly alter the specific activities of
            TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK
            ubiquitination and proteasomal degradation
REFERENCE   4  (bases 1 to 1358)
  AUTHORS   Li,Y., Sosnik,J., Brassard,L., Reese,M., Spiridonov,N.A.,
            Bates,T.C., Johnson,G.R., Anguita,J., Visconti,P.E. and
            Salicioni,A.M.
  TITLE     Expression and localization of five members of the testis-specific
            serine kinase (Tssk) family in mouse and human sperm and testis
  JOURNAL   Mol Hum Reprod 17 (1), 42-56 (2011)
   PUBMED   20729278
REFERENCE   5  (bases 1 to 1358)
  AUTHORS   Jha,K.N., Wong,L., Zerfas,P.M., De Silva,R.S., Fan,Y.X.,
            Spiridonov,N.A. and Johnson,G.R.
  TITLE     Identification of a novel HSP70-binding cochaperone critical to
            HSP90-mediated activation of small serine/threonine kinase
  JOURNAL   J Biol Chem 285 (45), 35180-35187 (2010)
   PUBMED   20829357
  REMARK    GeneRIF: HSP90 is essential for functional maturation of the kinase
            and identify SIP as a cochaperone that is critical to the
            HSP90-mediated activation of SSTK.
REFERENCE   6  (bases 1 to 1358)
  AUTHORS   Sosnik,J., Miranda,P.V., Spiridonov,N.A., Yoon,S.Y., Fissore,R.A.,
            Johnson,G.R. and Visconti,P.E.
  TITLE     Tssk6 is required for Izumo relocalization and gamete fusion in the
            mouse
  JOURNAL   J Cell Sci 122 (Pt 15), 2741-2749 (2009)
   PUBMED   19596796
REFERENCE   7  (bases 1 to 1358)
  AUTHORS   Spiridonov,N.A., Wong,L., Zerfas,P.M., Starost,M.F., Pack,S.D.,
            Paweletz,C.P. and Johnson,G.R.
  TITLE     Identification and characterization of SSTK, a serine/threonine
            protein kinase essential for male fertility
  JOURNAL   Mol Cell Biol 25 (10), 4250-4261 (2005)
   PUBMED   15870294
  REMARK    GeneRIF: results demonstrate that SSTK is required for proper
            postmeiotic chromatin remodeling and male fertility
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158564.7 and AF329484.1.
            
            On Nov 20, 2015 this sequence version replaced NM_032004.1.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC049542.1, AF329484.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC158564.7         96210-96240
            32-888              AF329484.1         1-857
            889-999             AC158564.7         97098-97208
            1000-1358           AF329484.1         969-1327
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.05 cM"
     gene            1..1358
                     /gene="Tssk6"
                     /gene_synonym="Sstk; TSK-6; TSSK-6"
                     /note="testis-specific serine kinase 6"
                     /db_xref="GeneID:83984"
                     /db_xref="MGI:MGI:2148775"
     exon            1..1335
                     /gene="Tssk6"
                     /gene_synonym="Sstk; TSK-6; TSSK-6"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..946
                     /gene="Tssk6"
                     /gene_synonym="Sstk; TSK-6; TSSK-6"
                     /EC_number="2.7.11.1"
                     /note="serine/threonine protein kinase SSTK;
                     testis-specific kinase 6; small serine/threonine kinase"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     6"
                     /protein_id="NP_114393.1"
                     /db_xref="CCDS:CCDS22354.1"
                     /db_xref="GeneID:83984"
                     /db_xref="MGI:MGI:2148775"
                     /translation="MSGDKLLSELGYKLGRTIGEGSYSKVKVATSKKYKGTVAIKVVD
                     RRRAPPDFVNKFLPRELSILRGVRHPHIVHVFEFIEVCNGKLYIVMEAAATDLLQAVQ
                     RNGRIPGSQARELFSQIAGAVRYLHDHHLVHRDLKCENVLLSPDERRVKITDFGFGRQ
                     AHGYPDLSTTYCGSAAYASPEVLLGIPYDPKKYDVWSLGVVLYVMVTGCMPFDDSDIA
                     GLPRRQKRGVLYPDGLELSERCKSLIAELLQFSPSARPSAGQVARNGWLRAGDSG"
     regulatory      1313..1318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tssk6"
                     /gene_synonym="Sstk; TSK-6; TSSK-6"
                     /note="hexamer: AATAAA"
     polyA_site      1335
                     /gene="Tssk6"
                     /gene_synonym="Sstk; TSK-6; TSSK-6"
                     /note="major polyA site"
ORIGIN      
        1 gggagtgggc acatgggggc acgggggtgc aggtgccaag caccatgggt taggcccgag
       61 attggagtcc ggccgccccc cgacagcagc cgcctcctgc tccccgcgcg ccccgggcgc
      121 caccatgtcg ggcgacaaac tcctgagcga acttggctat aagctgggcc gcacgatagg
      181 tgagggcagc tactccaagg tgaaagtggc cacctccaag aagtataaag ggacggtggc
      241 catcaaggtg gtggaccgcc ggcgagcgcc tccggacttc gtcaacaagt tcctgcctcg
      301 agagctgtcc atcctgcggg gagtacggca cccgcacatc gtacacgttt tcgagttcat
      361 cgaagtgtgc aatgggaagc tgtacatcgt gatggaggca gcagccaccg acctgctgca
      421 ggcagtgcag cgcaacgggc gcatcccggg gtcgcaggcg cgcgaactct tctcgcagat
      481 cgcgggcgcc gtgcgctacc tgcacgacca ccacctggtg caccgcgacc tcaagtgcga
      541 aaacgtgctg ctaagccctg acgagcgccg cgtcaagatc acggatttcg gtttcggccg
      601 ccaggcgcac ggctacccag acctgagcac cacctactgc ggctcggccg cgtatgcgtc
      661 acctgaggtg ctcctgggca tcccctacga ccctaagaaa tacgacgtgt ggagcctggg
      721 cgttgtgctc tacgtcatgg tcaccgggtg catgcccttc gacgactcgg acattgctgg
      781 cctaccccgg cgccagaagc gcggcgtgct ctaccctgac ggcctcgaac tgtcggaacg
      841 atgcaaatcc ttgatcgccg agctgctaca gttcagcccg tctgcccggc cctcggcggg
      901 ccaggtggcg cgcaatggct ggctgcgggc cggggactcc ggctaggagc cggggttctc
      961 gctgttcccg ccgcaccagg cacagagcca gggcggcgca cgcgcgaagg gcgagcttcg
     1021 ggaaacccgc gaagtcgccg cgcgccactg cgcacgcgtc ctgtcccttc cccttccccc
     1081 acggcggcgg ccctctgagg cctgctgttg ggttctgatt cctcctgcac aaactgattg
     1141 ctggagagcg catgcgcatc ctcgatttct ggcgagaagg ccccgggagg ggcgcaaaga
     1201 gaagagagag aagcattgcg cctgcgcaga atgagctgtt gcacggtggg ttgccatgct
     1261 acagagaaac cgtgggccag ccgtgcacgc taagttccag gttggaacct gcaataaatt
     1321 cgctcttcct tgcaaaaaaa aaaaaaaaaa aaaaaaaa
//
