LOCUS       NM_001253888            1273 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus testis-specific serine kinase 4 (Tssk4), transcript
            variant 3, mRNA.
ACCESSION   NM_001253888
VERSION     NM_001253888.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Fu,G., Wei,Y., Wang,X. and Yu,L.
  TITLE     Phosphorylated testis-specific serine/threonine kinase 4 may
            phosphorylate Crem at Ser-117
  JOURNAL   Biosci Biotechnol Biochem 80 (6), 1088-1094 (2016)
   PUBMED   26940607
  REMARK    GeneRIF: Phosphorylated Tssk4 might participate in testis genes
            expressions by phosphorylating Crem at Ser-117.
REFERENCE   3  (bases 1 to 1273)
  AUTHORS   Wang,X., Li,H., Fu,G., Wang,Y., Du,S., Yu,L., Wei,Y. and Chen,S.
  TITLE     Testis-specific serine/threonine protein kinase 4 (Tssk4)
            phosphorylates Odf2 at Ser-76
  JOURNAL   Sci Rep 6, 22861 (2016)
   PUBMED   26961893
  REMARK    GeneRIF: Testis-specific serine/threonine protein kinase 4 (Tssk4)
            phosphorylates Odf2 at Ser-76
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1273)
  AUTHORS   Wang,X., Wei,Y., Fu,G., Li,H., Saiyin,H., Lin,G., Wang,Z., Chen,S.
            and Yu,L.
  TITLE     Tssk4 is essential for maintaining the structural integrity of
            sperm flagellum
  JOURNAL   Mol Hum Reprod 21 (2), 136-145 (2015)
   PUBMED   25361759
  REMARK    GeneRIF: findings reveal that Tssk4 is required for maintaining the
            structural integrity of sperm flagellum and male fertility.
REFERENCE   5  (bases 1 to 1273)
  AUTHORS   Jha,K.N., Coleman,A.R., Wong,L., Salicioni,A.M., Howcroft,E. and
            Johnson,G.R.
  TITLE     Heat shock protein 90 functions to stabilize and activate the
            testis-specific serine/threonine kinases, a family of kinases
            essential for male fertility
  JOURNAL   J Biol Chem 288 (23), 16308-16320 (2013)
   PUBMED   23599433
  REMARK    GeneRIF: HSP90 function abolished the catalytic activities of TSSK4
            and -6 but did not significantly alter the specific activities of
            TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK
            ubiquitination and proteasomal degradation
REFERENCE   6  (bases 1 to 1273)
  AUTHORS   Wei,Y., Wang,X., Fu,G. and Yu,L.
  TITLE     Testis specific serine/threonine kinase 4 (Tssk4) maintains its
            kinase activity by phosphorylating itself at Thr-197
  JOURNAL   Mol Biol Rep 40 (1), 439-447 (2013)
   PUBMED   23054012
  REMARK    GeneRIF: autophosphorylation at Thr-197 plays a critical role in
            maintaining Tssk4 kinase activity, which might be involved in
            spermiogenesis.
REFERENCE   7  (bases 1 to 1273)
  AUTHORS   Li,Y., Sosnik,J., Brassard,L., Reese,M., Spiridonov,N.A.,
            Bates,T.C., Johnson,G.R., Anguita,J., Visconti,P.E. and
            Salicioni,A.M.
  TITLE     Expression and localization of five members of the testis-specific
            serine kinase (Tssk) family in mouse and human sperm and testis
  JOURNAL   Mol Hum Reprod 17 (1), 42-56 (2011)
   PUBMED   20729278
REFERENCE   8  (bases 1 to 1273)
  AUTHORS   Wei,Y., Fu,G., Hu,H., Lin,G., Yang,J., Guo,J., Zhu,Q. and Yu,L.
  TITLE     Isolation and characterization of mouse testis specific
            serine/threonine kinase 5 possessing four alternatively spliced
            variants
  JOURNAL   J Biochem Mol Biol 40 (5), 749-756 (2007)
   PUBMED   17927909
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC174678.2.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the coding region, compared to variant 1. This
            results in a longer protein (isoform 3), compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF127820.1, DQ397205.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-390               AC174678.2         10592-10981         c
            391-605             AC174678.2         10050-10264         c
            606-999             AC174678.2         9313-9706           c
            1000-1273           AC174678.2         8626-8899           c
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.19 cM"
     gene            1..1273
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /note="testis-specific serine kinase 4"
                     /db_xref="GeneID:71099"
                     /db_xref="MGI:MGI:1918349"
     exon            1..390
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1182
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /EC_number="2.7.11.1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     testis-specific serine/threonine-protein kinase 4;
                     testis-specific serine/threonine protein kinase 5 variant
                     beta; testis-specific serine/threonine protein kinase 5
                     variant gamma; testis-specific serine/threonine protein
                     kinase 5 variant delta; testis-specific serine/threonine
                     protein kinase 5 variant alpha; testis-specific kinase 4"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     4 isoform 3"
                     /protein_id="NP_001240817.1"
                     /db_xref="CCDS:CCDS88669.1"
                     /db_xref="GeneID:71099"
                     /db_xref="MGI:MGI:1918349"
                     /translation="MGKGDTSETASATPAYRSVMEEYGYEVGKIIGHGSYGTVYEAYY
                     TKQKVMVAVKIISKKKASEDYLNKFLPREIQVMKVLRHKYLINFYQAIETTSRVYIIL
                     ELAQGGDVLEWIQRYGACAETLAGKWFSQMALGIAYLHSKGIVHRLTPSLSAAGRDLK
                     LENLLLDKRENVKISDFGFAKMVPSSQPVHSSPSYRQMNSLSHLSQTYCGSFAYACPE
                     ILLGLPYNPFLSDTWSMGVILYTLVVARLPFDDTNLKKLLRETQKEVTFPANLTISQE
                     CKNLILQLLRQSTKRATILDVLRDPWMLKFQPEQPSNEIRLLEAMYQPTSSAKRHQSL
                     EITT"
     exon            391..605
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /inference="alignment:Splign:2.1.0"
     exon            606..999
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1273
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1247..1252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /note="hexamer: AATAAA"
     polyA_site      1269
                     /gene="Tssk4"
                     /gene_synonym="1700020B19Rik; 4933424F08Rik; TSK-4;
                     TSSK-4"
                     /note="major polyA site"
ORIGIN      
        1 aagttcttag tgaagggccg agcccagcat cccagccttg tgtgacttgg taaacactgg
       61 gaccctaact tcacttcagt accctccctt caggagctca aggtggcttc tccgtagaca
      121 gaggcttcta agtatcaaag ctccccactg cgcaagccca acatcatggg gaagggagac
      181 acctcggaaa cagcatcagc caccccagcc tatcgctctg tcatggagga gtatggttat
      241 gaggtgggca agatcattgg ccatggctcc tatggaactg tctatgaggc atactacaca
      301 aagcagaagg tcatggtggc tgtcaagatc atctcgaaga agaaggcctc tgaagactat
      361 ctcaacaagt tcctaccacg tgagatacag gtaatgaaag tcctacggca caagtacctc
      421 atcaacttct atcaggccat tgagaccaca tcccgagtat acatcatttt ggagctggct
      481 cagggcggtg atgtcctcga atggatccaa cgatatgggg cctgtgctga gacccttgct
      541 ggcaagtggt tctcccagat ggctttgggc atcgcctacc tgcacagcaa gggcatcgtg
      601 caccgcctga cccccagcct ttctgctgct ggtagggatt taaagttgga gaacctgttg
      661 ctggacaagc gggagaatgt gaagatatcg gactttggct tcgccaagat ggtgccttct
      721 agccagcctg tgcatagtag cccttcttac cgccaaatga acagcctttc ccacctcagc
      781 cagacctact gtggcagctt tgcttacgcc tgcccggaga tcttgctagg cttgccctac
      841 aaccctttcc tgtctgacac ctggagcatg ggcgtcatcc tctacactct agtggttgca
      901 cggctgccct ttgatgacac caatctcaag aagctgctga gagaaaccca gaaggaggtc
      961 actttcccag ctaacttgac catctcccag gagtgcaaga acctgatcct ccagctgcta
     1021 cgccaatcta ccaagcgtgc caccatccta gatgtcctca gggacccctg gatgctcaag
     1081 ttccagcctg agcaaccttc gaatgaaatc aggctgctcg aggccatgta ccaacccacc
     1141 agctctgcta aacggcacca gtccttggaa atcacaacct gaaagtggct gaggcaggag
     1201 gccaaggaaa aaagcaagaa agactggggg gaaatctttt ctataaaata aacctaatta
     1261 gtttcaccac gta
//
