LOCUS       NM_009436               1403 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus testis-specific serine kinase 2 (Tssk2), mRNA.
ACCESSION   NM_009436
VERSION     NM_009436.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Jha,K.N., Coleman,A.R., Wong,L., Salicioni,A.M., Howcroft,E. and
            Johnson,G.R.
  TITLE     Heat shock protein 90 functions to stabilize and activate the
            testis-specific serine/threonine kinases, a family of kinases
            essential for male fertility
  JOURNAL   J Biol Chem 288 (23), 16308-16320 (2013)
   PUBMED   23599433
  REMARK    GeneRIF: HSP90 function abolished the catalytic activities of TSSK4
            and -6 but did not significantly alter the specific activities of
            TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK
            ubiquitination and proteasomal degradation
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Yang,M.H., Su,J.Z., Liu,J. and Li,Y.H.
  TITLE     [Cloning and expression of Tssk1 & Tssk2 in mice and the presence &
            localization of them in mature sperm]
  JOURNAL   Dongwuxue Yanjiu 33 (4), 381-388 (2012)
   PUBMED   22855445
  REMARK    GeneRIF: Tssk1 and Tssk2 play an important role in the regulation
            of sperm function.
REFERENCE   3  (bases 1 to 1403)
  AUTHORS   Li,Y., Sosnik,J., Brassard,L., Reese,M., Spiridonov,N.A.,
            Bates,T.C., Johnson,G.R., Anguita,J., Visconti,P.E. and
            Salicioni,A.M.
  TITLE     Expression and localization of five members of the testis-specific
            serine kinase (Tssk) family in mouse and human sperm and testis
  JOURNAL   Mol Hum Reprod 17 (1), 42-56 (2011)
   PUBMED   20729278
REFERENCE   4  (bases 1 to 1403)
  AUTHORS   Shang,P., Baarends,W.M., Hoogerbrugge,J., Ooms,M.P., van
            Cappellen,W.A., de Jong,A.A., Dohle,G.R., van Eenennaam,H.,
            Gossen,J.A. and Grootegoed,J.A.
  TITLE     Functional transformation of the chromatoid body in mouse
            spermatids requires testis-specific serine/threonine kinases
  JOURNAL   J Cell Sci 123 (Pt 3), 331-339 (2010)
   PUBMED   20053632
  REMARK    GeneRIF: Results reveal TSSK1- and TSSK2-dependent functions of a
            transformed chromatoid body in post-meiotic cytodifferentiation of
            spermatids.
REFERENCE   5  (bases 1 to 1403)
  AUTHORS   Xu,B., Hao,Z., Jha,K.N., Zhang,Z., Urekar,C., Digilio,L.,
            Pulido,S., Strauss,J.F. 3rd, Flickinger,C.J. and Herr,J.C.
  TITLE     Targeted deletion of Tssk1 and 2 causes male infertility due to
            haploinsufficiency
  JOURNAL   Dev Biol 319 (2), 211-222 (2008)
   PUBMED   18533145
  REMARK    GeneRIF: TSSK1 and TSKS persist in sperm, define a critical role of
            TSKS' N-terminus in enzyme interaction, identify Ser 281 as a TSKS
            phosphorylation site and indicate an indispensable role for TSSK 1
            and 2 in spermiogenesis.
REFERENCE   6  (bases 1 to 1403)
  AUTHORS   Sutherland,H.F., Kim,U.J. and Scambler,P.J.
  TITLE     Cloning and comparative mapping of the DiGeorge syndrome critical
            region in the mouse
  JOURNAL   Genomics 52 (1), 37-43 (1998)
   PUBMED   9740669
REFERENCE   7  (bases 1 to 1403)
  AUTHORS   Nayak,S., Galili,N. and Buck,C.A.
  TITLE     Immunohistochemical analysis of the expression of two
            serine-threonine kinases in the maturing mouse testis
  JOURNAL   Mech Dev 74 (1-2), 171-174 (1998)
   PUBMED   9651519
  REMARK    GeneRIF: The expression patterns of Tsk2 is documented throughout
            spermiogenesis showing a late association of Tsk2 with tail-like
            structures in the lumen of the seminiferous tubule.
REFERENCE   8  (bases 1 to 1403)
  AUTHORS   Kueng,P., Nikolova,Z., Djonov,V., Hemphill,A., Rohrbach,V.,
            Boehlen,D., Zuercher,G., Andres,A.C. and Ziemiecki,A.
  TITLE     A novel family of serine/threonine kinases participating in
            spermiogenesis
  JOURNAL   J Cell Biol 139 (7), 1851-1859 (1997)
   PUBMED   9412477
REFERENCE   9  (bases 1 to 1403)
  AUTHORS   Reeves,R.H., Rue,E.E., Citron,M.P. and Cabin,D.E.
  TITLE     High-resolution recombinational map of mouse chromosome 16
  JOURNAL   Genomics 43 (2), 202-208 (1997)
   PUBMED   9244437
REFERENCE   10 (bases 1 to 1403)
  AUTHORS   Galili,N., Baldwin,H.S., Lund,J., Reeves,R., Gong,W., Wang,Z.,
            Roe,B.A., Emanuel,B.S., Nayak,S., Mickanin,C., Budarf,M.L. and
            Buck,C.A.
  TITLE     A region of mouse chromosome 16 is syntenic to the DiGeorge,
            velocardiofacial syndrome minimal critical region
  JOURNAL   Genome Res 7 (1), 17-26 (1997)
   PUBMED   9037598
  REMARK    Erratum:[Genome Res. 1997 Apr;7(4):399.. PMID: 9110179]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY714760.1, BC061175.1 and BC049618.1.
            
            On Aug 18, 2006 this sequence version replaced NM_009436.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BY714760.1         1-72
            73-1293             BC061175.1         72-1292
            1294-1403           BC049618.1         1277-1386
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 11.09 cM"
     gene            1..1403
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /note="testis-specific serine kinase 2"
                     /db_xref="GeneID:22115"
                     /db_xref="MGI:MGI:1347559"
     exon            1..1388
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1175
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /EC_number="2.7.11.1"
                     /note="serine/threonine kinase 22B (spermiogenesis
                     associated); TSSK-2; testis-specific kinase 2;
                     serine/threonine-protein kinase 22B; TSK-2; testis
                     specific serine/ threonine kinase 2"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     2"
                     /protein_id="NP_033462.2"
                     /db_xref="CCDS:CCDS28012.1"
                     /db_xref="GeneID:22115"
                     /db_xref="MGI:MGI:1347559"
                     /translation="MDDAAVLRKKGYIVGINLGKGSYAKVKSAYSERLKFNVAVKIID
                     RKKTPTDFVERFLPREMDILATVNHRSIIKTYEIFETSDGRIYIVMELGVQGDLLEFI
                     KCRGALHEDVARKMFRQLSSAVKYCHDLDVVHRDLKCENLLLDKDFNIKLSDFGFSKR
                     CLRDGSGRIVLSKTFCGSAAYAAPEVLQGIPYQPKVYDIWSLGVILYIMVCGSMPYDD
                     SDIKKMLRIQKEHRVDFPRSKNLTGECKDLIYRILQPDVNRRLHIDEILSHSWLQPPK
                     PKAMSSASFKREGEGKYRADCKLDTRPGSRPEHRPDHKLATKPQQRMLVTPENEDRME
                     DRLAETSRAKDHHISGAEVEKAST"
     regulatory      1288..1293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /note="hexamer: ATTAAA"
     polyA_site      1308
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /note="major polyA site"
     regulatory      1363..1368
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
                     /note="hexamer: AATAAA"
     polyA_site      1388
                     /gene="Tssk2"
                     /gene_synonym="DGS-G; SPOGA2; Stk22b; Tsk2"
ORIGIN      
        1 tagccagacg cctccccaca gtgggaatga ggacaatgcc tgctggcccg tgtggggagg
       61 ggatgtagag ggcggcggca ccggccgctc ctggcagcat ggacgatgcg gcggtcctaa
      121 ggaagaaggg ttacatcgtg ggaatcaatc tgggcaaggg ctcctacgca aaagtcaaat
      181 ctgcctactc agagcgcctc aagttcaatg tggcagtcaa gatcatagac cgcaagaaaa
      241 cacccactga ctttgtggag agattccttc ctagggagat ggacatcctg gcgaccgtca
      301 accaccgttc catcattaag acctacgaga tctttgagac ctctgatgga cgcatctaca
      361 ttgtcatgga gctgggcgtc cagggcgacc tcctcgagtt catcaagtgc cgaggagccc
      421 tgcatgagga tgtggcacgc aagatgttcc gccagctctc ctcagccgtc aagtactgcc
      481 acgacctgga tgtcgtccac cgagacctca aatgtgagaa ccttctgctt gacaaggact
      541 tcaacatcaa actgtctgac ttcggcttct ccaagcgctg cctgcgggac gggagtgggc
      601 gcatcgtcct cagcaaaacc ttctgtgggt cagcagctta tgcagccccc gaggtgctgc
      661 agggcatccc ctaccagccc aaggtgtatg acatctggag cctgggtgtg attctctaca
      721 tcatggtctg tggctccatg ccctacgatg actctgacat caaaaagatg ctgcgcatcc
      781 agaaggagca ccgagtggac ttcccacgct ccaagaacct aactggtgag tgcaaggacc
      841 tcatctaccg catcctacag ccagatgtca accggcggct gcacattgat gagatcctta
      901 gccactcatg gctacagccc cccaagccca aagccatgtc ttctgcctcc ttcaagaggg
      961 agggggaagg caagtatcga gctgattgca agttggatac tcgaccaggc tccagacccg
     1021 aacaccggcc tgaccacaag ctggcgacca aaccccagca acggatgctg gtgacacccg
     1081 agaatgaaga caggatggaa gacaggctgg ctgagacttc cagagctaaa gaccatcaca
     1141 tttctggagc tgaggtggag aaagcaagta cctagtggtg gagagggccc tgggggtatc
     1201 gtggtgtgca gtctgcaccc acataagcta agtaggcagg taggagctga agaaggcaca
     1261 ggtgcaagga acaagtaaaa ttcgtcaatt aaaccactat tttgattacg ttctattagc
     1321 tttcttccac ttagtagcaa agccattaat tactgaccac caaataaacc acaaagtgta
     1381 tacaagtaaa aaaaaaaaaa aaa
//
