LOCUS       NM_146173               2018 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus tetraspanin 33 (Tspan33), transcript variant 1, mRNA.
ACCESSION   NM_146173
VERSION     NM_146173.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Shah,J., Rouaud,F., Guerrera,D., Vasileva,E., Popov,L.M.,
            Kelley,W.L., Rubinstein,E., Carette,J.E., Amieva,M.R. and Citi,S.
  TITLE     A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to
            Promote alpha-Toxin Cytotoxicity
  JOURNAL   Cell Rep 25 (8), 2132-2147 (2018)
   PUBMED   30463011
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Ruiz-Garcia,A., Lopez-Lopez,S., Garcia-Ramirez,J.J., Baladron,V.,
            Ruiz-Hidalgo,M.J., Lopez-Sanz,L., Ballesteros,A., Laborda,J.,
            Monsalve,E.M. and Diaz-Guerra,M.J.
  TITLE     The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage
            Activation through Modulation of NOTCH Signaling
  JOURNAL   J Immunol 197 (8), 3371-3381 (2016)
   PUBMED   27574297
  REMARK    GeneRIF: The results suggest that TSPAN33 represents a new control
            element in the development of inflammation by macrophages that
            could constitute a potential therapeutic target.
REFERENCE   3  (bases 1 to 2018)
  AUTHORS   Haining,E.J., Yang,J., Bailey,R.L., Khan,K., Collier,R., Tsai,S.,
            Watson,S.P., Frampton,J., Garcia,P. and Tomlinson,M.G.
  TITLE     The TspanC8 subgroup of tetraspanins interacts with A disintegrin
            and metalloprotease 10 (ADAM10) and regulates its maturation and
            cell surface expression
  JOURNAL   J Biol Chem 287 (47), 39753-39765 (2012)
   PUBMED   23035126
REFERENCE   4  (bases 1 to 2018)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2018)
  AUTHORS   Garcia-Espana,A., Chung,P.J., Sarkar,I.N., Stiner,E., Sun,T.T. and
            Desalle,R.
  TITLE     Appearance of new tetraspanin genes during vertebrate evolution
  JOURNAL   Genomics 91 (4), 326-334 (2008)
   PUBMED   18291621
REFERENCE   6  (bases 1 to 2018)
  AUTHORS   Heikens,M.J., Cao,T.M., Morita,C., Dehart,S.L. and Tsai,S.
  TITLE     Penumbra encodes a novel tetraspanin that is highly expressed in
            erythroid progenitors and promotes effective erythropoiesis
  JOURNAL   Blood 109 (8), 3244-3252 (2007)
   PUBMED   17158226
  REMARK    GeneRIF: Penumbra has a positive function in erythropoiesis and its
            deletion or mutation may result in anemia.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF460925.1, AK004954.1,
            BE861472.1, AC069469.5 and BE949216.1.
            
            On Aug 19, 2014 this sequence version replaced NM_146173.2.
            
            Summary: This gene encodes a member of the tetraspanin family which
            typically have four transmembrane domains. The encoded protein may
            be involved in the regulation of erythropoiesis. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024685.1, AF276890.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BF460925.1         1-26
            27-698              AK004954.1         1-672
            699-699             BE861472.1         308-308
            700-935             AK004954.1         673-908
            936-936             BE861472.1         545-545
            937-1943            AK004954.1         910-1916
            1944-1944           AC069469.5         92238-92238
            1945-2000           AK004954.1         1918-1973
            2001-2018           BE949216.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.36 cM"
     gene            1..2018
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /note="tetraspanin 33"
                     /db_xref="GeneID:232670"
                     /db_xref="MGI:MGI:1919012"
     exon            1..331
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..1081
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /note="isoform 1 is encoded by transcript variant 1;
                     penumbra; proerythroblast new membrane"
                     /codon_start=1
                     /product="tetraspanin-33 isoform 1"
                     /protein_id="NP_666285.1"
                     /db_xref="CCDS:CCDS19964.1"
                     /db_xref="GeneID:232670"
                     /db_xref="MGI:MGI:1919012"
                     /translation="MARRPGVPAAYGDEFSFVSPLVKYLLFFFNMLFWVISMVMVAVG
                     VYARLMKHAEAALACLAVDPAILLIVVGVLMFLLTFCGCIGSLRENICLLQTFSLCLT
                     IVFLLQLAAGILGFVFSDKARGKVSEIINNAIVHYRDDLDLQNLIDFGQKKFSCCGGI
                     SYRDWSQNMYFNCSEDNPSRERCSVPYSCCLPTPNQAVINTMCGQGMQALDYLEASKV
                     IYTNGCIDKLVNWIHSNLFLLGGVALGLAIPQLVGILLSQVLVNQIKDQIKLQLYNQQ
                     HRADPWY"
     exon            332..389
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            390..517
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            518..592
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            593..688
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            689..817
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            818..979
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     exon            980..2001
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /note="hexamer: AATAAA"
     polyA_site      2000
                     /gene="Tspan33"
                     /gene_synonym="1300010A20Rik; Pen"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgcgg tggagtctcc gctgctcacg ctgcccgccg cggcttcagc ggcggcgccg
       61 ttccctggcc gcgcggctcc ccgacgcacg gttcgggcgg ctcccggcgc ggcgcggctg
      121 ccccggcgtt cgggtccccg tggccccgcg gctcccgccc cgggctgcag ggcgcacagg
      181 tgggccggtg gccgctggga gataggcccc ccgggggcag cggcggacca tggcgcggag
      241 acctggcgtg ccggctgcct acggggacga gttctccttc gtcagcccgc tggtgaaata
      301 cctgctcttc ttcttcaaca tgctcttctg ggtaatctcc atggtcatgg tggccgtggg
      361 tgtctatgcc aggctaatga agcacgcaga agcagccttg gcttgtctgg cggtggaccc
      421 tgctatcctg ctgatcgtgg tgggcgtcct catgttcctg ctcaccttct gcggctgtat
      481 cggatccctt cgggagaaca tctgccttct gcagacgttc tccctctgcc tcaccatcgt
      541 gttcctgctg cagctggctg ctggcatcct gggcttcgtc ttctcagaca aggctcgggg
      601 gaaagtgagc gagatcatca acaatgctat cgtgcattat cgagatgacc tggatctgca
      661 gaacctcatc gactttggcc agaagaagtt cagctgctgt ggagggatct cctacagaga
      721 ctggtctcag aatatgtact tcaactgctc ggaggacaac cctagccggg agcgctgctc
      781 tgtgccttat tcctgttgct tgcccacccc caaccaggca gtgatcaaca cgatgtgtgg
      841 ccaaggtatg caggctctgg actacttgga agctagtaag gtcatctaca ccaatggctg
      901 tattgacaag ttggtcaact ggatacacag caacctgttc ctgcttggtg gtgtggcact
      961 aggcctggcc atcccccagc tggtgggaat cctgctatcc caggtcctcg tgaatcaaat
     1021 aaaagatcag atcaagctac agctctacaa ccaacagcac cgagctgacc cgtggtactg
     1081 agaatccttc ctgcgcctcc ttaccatggc gacgagcatc tcacggacca gcagcagagg
     1141 gtgctgagag cacccagtgc agatttggat tccagccccc aaatgagggc ccagtggaaa
     1201 gaagcaagct ccagaaggca ggacacagat ggctcaggtc tccgaaagat gtgcctcatc
     1261 tccccatcct agcctcagca ttgtccgagg tttatttcat gctgtttgta accttgaaca
     1321 tttgagtttg tgtttttagt ttcctctggg cagaggtgtg gaaactgcag cggcacagaa
     1381 agctgtagga ttactagctg catactgagg cacaacagac agctctgtta gggctgctgc
     1441 tgagctaggt ggacagagtc tgctgccagg gagtcctggc tgtgcacaga tactggcagc
     1501 cctgcaggag tccagttggc acaattccac ctccagagag gctgggagtg cagcgggcag
     1561 agcagcggga gcctagggaa cctaggggtc ggatcgggac cgcagggcac atgggggata
     1621 ggagtgaagg gggtatgttt accaaatgct tgcctagccg cactcccagg tagtcagagt
     1681 gagctatatc ctgctcctcc ctcgtctcca tggaaacaag gctgcaaggg caaggggtat
     1741 aacagcagcc aaattcatcc tcacccgctc tgtaaaatgt ttggaaccgt ggtccctaaa
     1801 ttctgcgtgc cggactaagc catacatgcc cttgaattcc tctctgtctg gccttcccgc
     1861 cccagcaagt ggggttttct ttaacttggc agaggagtgg caccaccacc cacccgcatc
     1921 ccctgcacca gaactcagtg tttctacagt gtaagaatcg ggaatctggc agaaatggca
     1981 ataaaagttt gttgacatgg gaaaaaaaaa aaaaaaaa
//
