LOCUS       NM_020286               1697 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tetraspanin 32 (Tspan32), transcript variant 2, mRNA.
ACCESSION   NM_020286
VERSION     NM_020286.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Qiu,Q., Sun,Y., Yang,L., Li,Q., Feng,Y., Li,M., Yin,Y., Zheng,L.,
            Li,N., Qiu,H., Cui,X., He,W., Wang,B., Pan,C., Wang,Z., Huang,J.,
            Sample,K.M., Li,Z. and Hu,Y.
  TITLE     TSPAN32 suppresses chronic myeloid leukemia pathogenesis and
            progression by stabilizing PTEN
  JOURNAL   Signal Transduct Target Ther 8 (1), 90 (2023)
   PUBMED   36854750
  REMARK    GeneRIF: TSPAN32 suppresses chronic myeloid leukemia pathogenesis
            and progression by stabilizing PTEN.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1697)
  AUTHORS   Makkawi,M., Howells,D., Wright,M.D. and Jackson,D.E.
  TITLE     A complementary role for tetraspanin superfamily member TSSC6 and
            ADP purinergic P2Y12 receptor in platelets
  JOURNAL   Thromb Res 161, 12-21 (2018)
   PUBMED   29178985
  REMARK    GeneRIF: Study demonstrates a complementary role between TSSC6 and
            P2Y12 receptor in platelets in regulating 'outside in' integrin
            alphaIIbbeta3 signalling thrombus growth and stability.
REFERENCE   4  (bases 1 to 1697)
  AUTHORS   Gartlan,K.H., Belz,G.T., Tarrant,J.M., Minigo,G., Katsara,M.,
            Sheng,K.C., Sofi,M., van Spriel,A.B., Apostolopoulos,V.,
            Plebanski,M., Robb,L. and Wright,M.D.
  TITLE     A complementary role for the tetraspanins CD37 and Tssc6 in
            cellular immunity
  JOURNAL   J Immunol 185 (6), 3158-3166 (2010)
   PUBMED   20709950
REFERENCE   5  (bases 1 to 1697)
  AUTHORS   Mohammad,F., Mondal,T., Guseva,N., Pandey,G.K. and Kanduri,C.
  TITLE     Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by
            interacting with Dnmt1
  JOURNAL   Development 137 (15), 2493-2499 (2010)
   PUBMED   20573698
REFERENCE   6  (bases 1 to 1697)
  AUTHORS   Engemann,S., Strodicke,M., Paulsen,M., Franck,O., Reinhardt,R.,
            Lane,N., Reik,W. and Walter,J.
  TITLE     Sequence and functional comparison in the Beckwith-Wiedemann
            region: implications for a novel imprinting centre and extended
            imprinting
  JOURNAL   Hum Mol Genet 9 (18), 2691-2706 (2000)
   PUBMED   11063728
REFERENCE   7  (bases 1 to 1697)
  AUTHORS   Onyango,P., Miller,W., Lehoczky,J., Leung,C.T., Birren,B.,
            Wheelan,S., Dewar,K. and Feinberg,A.P.
  TITLE     Sequence and comparative analysis of the mouse 1-megabase region
            orthologous to the human 11p15 imprinted domain
  JOURNAL   Genome Res 10 (11), 1697-1710 (2000)
   PUBMED   11076855
  REMARK    Erratum:[Genome Res 2001 Feb;11(2):308]
REFERENCE   8  (bases 1 to 1697)
  AUTHORS   Nicholson,R.H., Pantano,S., Eliason,J.F., Galy,A., Weiler,S.,
            Kaplan,J., Hughes,M.R. and Ko,M.S.
  TITLE     Phemx, a novel mouse gene expressed in hematopoietic cells maps to
            the imprinted cluster on distal chromosome 7
  JOURNAL   Genomics 68 (1), 13-21 (2000)
   PUBMED   10950922
REFERENCE   9  (bases 1 to 1697)
  AUTHORS   Paulsen,M., El-Maarri,O., Engemann,S., Strodicke,M., Franck,O.,
            Davies,K., Reinhardt,R., Reik,W. and Walter,J.
  TITLE     Sequence conservation and variability of imprinting in the
            Beckwith-Wiedemann syndrome gene cluster in human and mouse
  JOURNAL   Hum Mol Genet 9 (12), 1829-1841 (2000)
   PUBMED   10915772
REFERENCE   10 (bases 1 to 1697)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY208601.1 and AF291425.1.
            
            On Jun 3, 2008 this sequence version replaced NM_020286.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154694.1, AF291425.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BY208601.1         19-50
            33-1697             AF291425.1         1-1665
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.09 cM"
     gene            1..1697
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /note="tetraspanin 32"
                     /db_xref="GeneID:27027"
                     /db_xref="MGI:MGI:1350360"
     exon            1..360
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..950
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /note="isoform b is encoded by transcript variant 2;
                     tspan-32; protein Phemx; AML1-regulated transmembrane
                     protein 1; tumor-suppressing subchromosomal transferable
                     fragment 6; pan hematopoietic expression"
                     /codon_start=1
                     /product="tetraspanin-32 isoform b"
                     /protein_id="NP_064682.1"
                     /db_xref="CCDS:CCDS22037.1"
                     /db_xref="GeneID:27027"
                     /db_xref="MGI:MGI:1350360"
                     /translation="MATMVVVIHFGDHFTVIGHASLERNPYETLRYWAFYVGISLAGL
                     LSLGAALSTIATVREAHGLMAAGFLCFALSFCILVQVAFWRFYNPTQVEDAVLDTYDF
                     VYDQAMKSPSSNWWQELAVIQDTFLCCGKKSPFGLLVSTGAIMCQGREAMREDCLQSI
                     RNVLWTHYSIASILTCTSLALTVYAMMLCAFLWFAIHSYHGLDRKGRYSLTPPRSHGF
                     QTQEPSLFRWT"
     exon            361..458
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            459..533
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            534..635
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            636..722
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            723..806
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            807..901
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1697
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1672..1677
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /note="hexamer: AATAAA"
     polyA_site      1697
                     /gene="Tspan32"
                     /gene_synonym="Art-1; D7Wsu37e; Phemx; Tssc6"
                     /note="major polyA site"
ORIGIN      
        1 gagaggagct gagcaccatg gggcactggc tagtgtgttt tctacttatc tctgtgtgta
       61 gtacactatc tgtgagtgcc tgcccacaga aatatgtagg cctcctgtag ctgccttgtt
      121 cctgtgcacc aaggaagctg gagggagcca ggctgggggc ctaagaagcc cagaccctat
      181 attctgtcag ggtgaggggc ctctgcaact ccccagcgct gacagtgagc ccacttcacc
      241 ctcagctgct gggcctctct atggccacca tggtggttgt cattcacttt ggagaccact
      301 ttactgtgat tggccatgca tcgttggaga ggaaccccta tgaaacactg cgctattggg
      361 ccttctatgt ggggatcagc ctagcggggc tcctgagcct gggagctgct ctgagcacca
      421 tagccactgt gagggaggcc catggcctca tggctgcggg tttcctgtgc tttgccctgt
      481 cattctgcat cctggtgcag gtggccttct ggagattcta caaccccacc caggtggagg
      541 atgcggtgtt ggacacctat gacttcgtgt atgatcaggc aatgaagagc ccatctagca
      601 actggtggca ggagctggcc gtcatccagg acacgtttct gtgttgtggg aagaaatctc
      661 cttttgggct tctggtgagc actggagcca tcatgtgtca gggccgggag gccatgagag
      721 aggactgcct ccagagcatc aggaacgttc tgtggacaca ctacagtatt gcttccatcc
      781 tgacctgcac cagcctggcc ctcacggtat atgctatgat gctctgtgcc ttcctgtggt
      841 ttgccattca ctcctaccat ggcttggata gaaaaggcag atactccctg acaccaccaa
      901 gatcccatgg tttccagacc caggaaccca gcctcttcag atggacctag gatgggctgg
      961 tgcctcaatg ccctgctgaa gtgcacatgt ttgctaactg ttcaacaacc ctctaaagca
     1021 tgagtgtttg ctgacaattg ggtctagcag gatgcttgct gcctgcttgc ccacagagat
     1081 gaagtctttg gcttcacatg atcctcaaag gagtagataa actctgtctt gcacatggtg
     1141 gggcaagaga aggggagaat atcccaccag gtgtgttagt taatcttaca ttgttatggc
     1201 cagatactta ccagaaacag gaacgattta ttctggctca tgatttcaga gacagactct
     1261 ggtccatcac aacaggggag gcatggagga gtggctatgt ctatggcatc aggaacataa
     1321 ggttgaagct agatgttgag gtcaaccaat ttcctgtggg tcatcatgga gaaaagcatg
     1381 actttggctc tgtagtcatg ctactgaaat gtgcagaacc ccatggtgac agccatgttt
     1441 tggaaatggc catggggctg ctaatgcttc ctagtttaga ggtcactgta tcttcacctg
     1501 gattccagct acctctccct cctgctcagt aactgttagg gacttgggag atgctggact
     1561 gtgacaagtt agctgaagcc aggactccat gatccagaat ccctgaggtg aggcgtttag
     1621 ttgatgcgtg tttgggatta cccacactcc ttataagtaa ctcctctccc taataaaccc
     1681 attgattcta attgaaa
//
