LOCUS       NM_027533               4261 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tetraspanin 2 (Tspan2), transcript variant 1, mRNA.
ACCESSION   NM_027533
VERSION     NM_027533.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Liu,J., Chanumolu,S.K., White,K.M., Albahrani,M., Otu,H.H. and
            Nawshad,A.
  TITLE     Transcriptional analysis of cleft palate in TGFbeta3 mutant mice
  JOURNAL   Sci Rep 10 (1), 14940 (2020)
   PUBMED   32913205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   de Monasterio-Schrader,P., Patzig,J., Mobius,W., Barrette,B.,
            Wagner,T.L., Kusch,K., Edgar,J.M., Brophy,P.J. and Werner,H.B.
  TITLE     Uncoupling of neuroinflammation from axonal degeneration in mice
            lacking the myelin protein tetraspanin-2
  JOURNAL   Glia 61 (11), 1832-1847 (2013)
   PUBMED   24038504
  REMARK    GeneRIF: the activation of astrocytes and microglia was strongly
            enhanced in Tspan2(null) *Plp1(null) double-mutants compared with
            either single-mutant
REFERENCE   3  (bases 1 to 4261)
  AUTHORS   Tripathi,P., Wang,Y., Casey,A.M. and Chen,F.
  TITLE     Absence of canonical Smad signaling in ureteral and bladder
            mesenchyme causes ureteropelvic junction obstruction
  JOURNAL   J Am Soc Nephrol 23 (4), 618-628 (2012)
   PUBMED   22282597
REFERENCE   4  (bases 1 to 4261)
  AUTHORS   Wansleeben,C., Feitsma,H., Montcouquiol,M., Kroon,C., Cuppen,E. and
            Meijlink,F.
  TITLE     Planar cell polarity defects and defective Vangl2 trafficking in
            mutants for the COPII gene Sec24b
  JOURNAL   Development 137 (7), 1067-1073 (2010)
   PUBMED   20215345
REFERENCE   5  (bases 1 to 4261)
  AUTHORS   Gobert,R.P., Joubert,L., Curchod,M.L., Salvat,C., Foucault,I.,
            Jorand-Lebrun,C., Lamarine,M., Peixoto,H., Vignaud,C., Fremaux,C.,
            Jomotte,T., Francon,B., Alliod,C., Bernasconi,L., Abderrahim,H.,
            Perrin,D., Bombrun,A., Zanoguera,F., Rommel,C. and Hooft van
            Huijsduijnen,R.
  TITLE     Convergent functional genomics of oligodendrocyte differentiation
            identifies multiple autoinhibitory signaling circuits
  JOURNAL   Mol Cell Biol 29 (6), 1538-1553 (2009)
   PUBMED   19139271
REFERENCE   6  (bases 1 to 4261)
  AUTHORS   Garcia-Espana,A., Chung,P.J., Sarkar,I.N., Stiner,E., Sun,T.T. and
            Desalle,R.
  TITLE     Appearance of new tetraspanin genes during vertebrate evolution
  JOURNAL   Genomics 91 (4), 326-334 (2008)
   PUBMED   18291621
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134871.3, AK079560.1 and AK018178.1.
            
            On Jul 27, 2011 this sequence version replaced NM_027533.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK079560.1, AK049888.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AC134871.3         136392-136587       c
            197-899             AK079560.1         1-703
            900-3182            AK018178.1         703-2985
            3183-4261           AC134871.3         99047-100125        c
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 45.25 cM"
     gene            1..4261
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="tetraspanin 2"
                     /db_xref="GeneID:70747"
                     /db_xref="MGI:MGI:1917997"
     exon            1..296
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..893
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tetraspan 2"
                     /codon_start=1
                     /product="tetraspanin-2 isoform 1"
                     /protein_id="NP_081809.2"
                     /db_xref="CCDS:CCDS17688.1"
                     /db_xref="GeneID:70747"
                     /db_xref="MGI:MGI:1917997"
                     /translation="MGRFRGGLRCIKYLLLGFNLLFWLAGSAVIAFGLWFRFGGTMKD
                     LSSEDKSPEYFYVGLYVLVGAGALMMTVGFFGCCGAMRESQCVLGSFFTCLLVIFAAE
                     VTTGVFAFIGKDVAIRHVQSMYEEAYSDYLKDRARGNGTLITFHSAFQCCGKESSEQV
                     QPTCPKELPGHKNCIDKIETVISAKLQLIGIVGIGIAGLTIFGMIFSMVLCCAIRNSR
                     DVI"
     exon            297..399
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            400..497
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            498..572
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            573..671
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            672..743
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            744..827
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     exon            828..4261
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3161..3166
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="hexamer: ATTAAA"
     polyA_site      3183
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="major polyA site"
     regulatory      4164..4169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="hexamer: AACAAA"
     polyA_site      4188
                     /gene="Tspan2"
                     /gene_synonym="6330415F13Rik; B230119D02Rik; tspan-2"
                     /note="major polyA site"
ORIGIN      
        1 gaaggtggtg ctacctccaa gggccggagc gggtgcgtgg gaccgggatc tccaccccag
       61 ggcgccccag ccacagccag ctggccgctc gcgaccaaat ccccgcccgc agaccacgcc
      121 cacaggtcac gcccctccca acccgccccg cccctccacc ggcgcccgcc gctcccgggg
      181 ctcggcgcgg gcctggggaa tcgggagccc gccgccgggg tcgcagcatg gggcgttttc
      241 gcgggggcct gcggtgcatc aagtatctgc tgctcggctt caacctgctg ttctggctag
      301 ccggatcagc cgttattgca tttggactat ggtttcgatt tggaggtacc atgaaggatt
      361 tatcatcaga ggacaagtcc ccagagtatt tctatgtggg gctgtatgtg ctggttggtg
      421 ctggggccct gatgatgact gtgggtttct tcgggtgctg tggagccatg cgtgagtcac
      481 agtgtgtgct cggatcattt tttacctgtc tgttggtgat atttgctgct gaagtaacca
      541 ctggagtatt tgcatttata ggcaaggatg tagctatacg acatgtacag agcatgtatg
      601 aggaggccta cagcgactac cttaaagaca gggcaagagg aaatggaact ctcattacct
      661 tccactcagc atttcagtgc tgtggaaaag aaagctctga gcaagtccag cccacgtgtc
      721 caaaggagct cccaggccac aagaattgca tcgacaaaat tgaaaccgta atcagtgcta
      781 aactgcagct cattggaatt gttggtattg ggattgcggg tctcacgatc tttggcatga
      841 tattcagcat ggtgctttgc tgtgcaatac ggaactcacg cgatgtgatc tgatgatact
      901 tcatcaggaa aattgcaatc tagagttgtc gtgctaatta tcacaagagc tctctcggct
      961 cgtttttaat attcaggata ttagaagcta aaataatttt ctgtcaactg tatatttgca
     1021 catgtatgta agtctgactc attactgatt tgccccttga gtgaatgtct gtttctggta
     1081 tatgcattgc acatactgat atctgtttcc tgggaaaccc tagttcttgt atgcaagaat
     1141 aacctattta agtaccagaa aaaaagcaat gagagatttt taaaactcgg ctattcagga
     1201 aatgtaataa tgggtccatt tctcagattt tagccatgaa aattagatac ataaagagaa
     1261 tgcaggattt ctagttaatg gaagcaataa gatagaagaa ttgaagggcc atagattgac
     1321 tgtgtctgag ttgagtgtaa agatttcttg aaatatatat atatatatac atatattctc
     1381 cccagaatcc agtaaaccac agttttagaa ctaaacacat ctgtaaaagt aaatatagca
     1441 tggaaaaatc taattcaaaa aagtcatgtt ttcttagcat ttaaaaacag aaagcctctc
     1501 tctggaaaga gaagtcacgc agacagacaa gcatgtgccc cagaaaagtg agtgctttaa
     1561 agactaaaac taacaacttt gaagggggag aaacactgct ataggatgac atgcaaacgt
     1621 tgttttctcc ctcttctctg ccaactctgc tttgaactag tgaaggcctc gccttcccac
     1681 aaggcctttc cttcaccctc cttactgttt ttttcagatg tggtgacaga ggaaagcagg
     1741 cactgtgcag cgtgacagtg ggggactctg gctggcactg tctgtacctc tggaatacct
     1801 catgacttga gtttacatac tttcctaata tttttccatt tttcttattt atctgtgcag
     1861 agaattcttt atagattaag aacatagttt tattctttaa aataaataaa tcgccccata
     1921 actcagatat tatgttatga gtaccctgtc taaaccaata taaacacctt cttcaatatg
     1981 tatgcacaca acaaaggcaa gttaatggat caaaagtact ttcgtgcata cagctgcagc
     2041 acaactatat tctttctcag cgaacccctg tggggatgaa tagatgctca ggttctttgg
     2101 gagcctgagt gattgatttt aagtggggaa acccaggcac tgctcggcct tttggcagca
     2161 agtgggaaac cccacagcag ggtcgaaatt gatgtatttg tcataatgga acagcgtata
     2221 aaggagtctt aggattatat tgaccacata aaagtgaagg catgaggaat tctggaaacc
     2281 gtcaggctag cttctatgtc cgctaagaac ctaagacttt ggtatgtgct agaacaaagt
     2341 gccatgcttc aggaaacttt taccaagtgt tttgctgagg acacacaact cctgggatgt
     2401 ctcacccaca gagcgccacc tctctctttt tcagatattc ttgctactgc tactagcttt
     2461 ggagaaaaaa actgagggag ttaaaagtat aactgtgtat gactacttta cctatgtatg
     2521 caaaagtatt taaaaaaaat cactctgtga tgtttatacc ccagtggttg acaaatgcca
     2581 gctttttaag caaacaaaga gtgatcagtc cttcttatag ctttagttta tctgtcttcc
     2641 tgaagacaga gaacaggctc aagtgtgtac tcaggttcct ccagctcaga gttatttccc
     2701 gttcctcaga agtgagtttc attccctggg tgagtgaata agtttaagga aaaccacagt
     2761 aactatagac agaagagtaa aataattttt taatgcagaa gaataagtct agaaacccat
     2821 gtcctgtgtg attacctctc tgagggtgaa gctttccaaa gaatgtgctg ggtaaaatta
     2881 gagaattcca tctttgagta aagatggata tatatcttta tgtgaatgga taaagctata
     2941 tctatagaga tatataaccc ctatgtcctg aacttataca tatcttaaat acatatctct
     3001 taaggctaaa gagatatgta atttaataca tgtgtacccg tttgaaacta tggagataaa
     3061 tgtttgtata catacacatg tacatgtata tacatttaca gttatttctg tttacctgaa
     3121 ctaatttgag atcagaccca aacattttaa gttattttaa attaaatttt ctatgtactt
     3181 acactgcttg agttatctta aaatcttagt gtatcttctt aacaagttca cattttctta
     3241 tttaaatgtg ttacctacca atttacccaa acagtatagt aaaaacaaac tggtaatcct
     3301 ttcatgctgt tgttgtgcag tgggttcaaa agcaacaccc ctagggttct aattacatat
     3361 agttgcgttt cagctaacac cagttcaccc aaattgtttc tccttcctcc agaaaaactt
     3421 ggttacctat ggcttagcct ttcgagttaa tcctgggtcc agtgtggttc tcctgtacca
     3481 acagccatcg tttccctctg aggaatctaa ccgatgtagc tgggtgagaa tgagacccag
     3541 agaaagatga taataatctc aataataatc aataaagaag atagcatgtt ctcccttctg
     3601 aaataagttt tctgaatatt caaagggtat ttgctctctt gtcataggtg aaggtggttt
     3661 ctatggccca gaggcaagcc actagtactc cccacagccc agctttcaag gccccactgg
     3721 catgctcttg aacaccaagc ccgtttcccc acacccagga aagagcaatt tatgtctctt
     3781 gagggtagaa ccagcgtaag ctcatctgaa tcaagttggt ttttgtgtgg attttaaaga
     3841 ctaaagattt cctactttct ttaaatatcc tcccccccct ttttttttac tataaaaggt
     3901 attttgtata tagatgtctt ttaaaagtga aaatactaca gcaatacaaa atgttattgt
     3961 ttggtgtcct tataacaagc aagggattca cagttcagac gctgccccct ggaaacaggg
     4021 aagacacagc aagtacaagt aacatgccct gtgcttccca gagcttggct gcagaaatgt
     4081 ggagttaata caacaggaac ctttttgtgt gttttgtggt gtttatcctg tatattttgt
     4141 gatttctttt actcctatta aacaacaaaa aaaaattagt taaagaaatt taaattgcat
     4201 gttaattatt aaataagtca ataaccaata tataagtact tacaatacag aggaactact
     4261 g
//
