LOCUS       NM_001411747            2520 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus tetraspanin 12 (Tspan12), transcript variant 3, mRNA.
ACCESSION   NM_001411747 XM_030255427
VERSION     NM_001411747.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Zhang,L., Abedin,M., Jo,H.N., Levey,J., Dinh,Q.C., Chen,Z.,
            Angers,S. and Junge,H.J.
  TITLE     A Frizzled4-LRP5 agonist promotes blood-retina barrier function by
            inducing a Norrin-like transcriptional response
  JOURNAL   iScience 26 (8), 107415 (2023)
   PUBMED   37559903
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2520)
  AUTHORS   Collin,G.B., Gogna,N., Chang,B., Damkham,N., Pinkney,J., Hyde,L.F.,
            Stone,L., Naggert,J.K., Nishina,P.M. and Krebs,M.P.
  TITLE     Mouse Models of Inherited Retinal Degeneration with Photoreceptor
            Cell Loss
  JOURNAL   Cells 9 (4), 931 (2020)
   PUBMED   32290105
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2520)
  AUTHORS   Cho,C., Wang,Y., Smallwood,P.M., Williams,J. and Nathans,J.
  TITLE     Dlg1 activates beta-catenin signaling to regulate retinal
            angiogenesis and the blood-retina and blood-brain barriers
  JOURNAL   Elife 8, e45542 (2019)
   PUBMED   31066677
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2520)
  AUTHORS   Wang,Y., Cho,C., Williams,J., Smallwood,P.M., Zhang,C., Junge,H.J.
            and Nathans,J.
  TITLE     Interplay of the Norrin and Wnt7a/Wnt7b signaling systems in
            blood-brain barrier and blood-retina barrier development and
            maintenance
  JOURNAL   Proc Natl Acad Sci U S A 115 (50), E11827-E11836 (2018)
   PUBMED   30478038
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3934. doi:
            10.1073/pnas.1901942116.. PMID: 30782804]
REFERENCE   6  (bases 1 to 2520)
  AUTHORS   Zhang,C., Lai,M.B., Pedler,M.G., Johnson,V., Adams,R.H.,
            Petrash,J.M., Chen,Z. and Junge,H.J.
  TITLE     Endothelial Cell-Specific Inactivation of TSPAN12 (Tetraspanin 12)
            Reveals Pathological Consequences of Barrier Defects in an
            Otherwise Intact Vasculature
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (11), 2691-2705 (2018)
   PUBMED   30354230
  REMARK    GeneRIF: TSPAN12 functions in endothelial cells to promote vascular
            morphogenesis and blood-retinal barrier formation in developing
            mice and BRB maintenance in adult mice.
REFERENCE   7  (bases 1 to 2520)
  AUTHORS   Ye,M., Wei,T., Wang,Q., Sun,Y., Tang,R., Guo,L. and Zhu,W.
  TITLE     TSPAN12 promotes chemoresistance and proliferation of SCLC under
            the regulation of miR-495
  JOURNAL   Biochem Biophys Res Commun 486 (2), 349-356 (2017)
   PUBMED   28302484
  REMARK    GeneRIF: TSPAN12 promotes chemoresistance and proliferation of
            small cell lung carcinoma under the regulation of miR-495.
REFERENCE   8  (bases 1 to 2520)
  AUTHORS   Poulter,J.A., Ali,M., Gilmour,D.F., Rice,A., Kondo,H., Hayashi,K.,
            Mackey,D.A., Kearns,L.S., Ruddle,J.B., Craig,J.E., Pierce,E.A.,
            Downey,L.M., Mohamed,M.D., Markham,A.F., Inglehearn,C.F. and
            Toomes,C.
  TITLE     Mutations in TSPAN12 cause autosomal-dominant familial exudative
            vitreoretinopathy
  JOURNAL   Am J Hum Genet 86 (2), 248-253 (2010)
   PUBMED   20159112
  REMARK    Erratum:[Am J Hum Genet. 2016 Mar 3;98(3):592. doi:
            10.1016/j.ajhg.2016.02.017.. PMID: 28863275]
REFERENCE   9  (bases 1 to 2520)
  AUTHORS   Junge,H.J., Yang,S., Burton,J.B., Paes,K., Shu,X., French,D.M.,
            Costa,M., Rice,D.S. and Ye,W.
  TITLE     TSPAN12 regulates retinal vascular development by promoting Norrin-
            but not Wnt-induced FZD4/beta-catenin signaling
  JOURNAL   Cell 139 (2), 299-311 (2009)
   PUBMED   19837033
  REMARK    GeneRIF: Data indicate that Norrin multimers and TSPAN12
            cooperatively promote multimerization of FZD4 and its associated
            proteins to elicit physiological levels of signaling.
REFERENCE   10 (bases 1 to 2520)
  AUTHORS   Garcia-Espana,A., Chung,P.J., Sarkar,I.N., Stiner,E., Sun,T.T. and
            Desalle,R.
  TITLE     Appearance of new tetraspanin genes during vertebrate evolution
  JOURNAL   Genomics 91 (4), 326-334 (2008)
   PUBMED   18291621
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122421.4 and AC163104.2.
            
            On Sep 1, 2022 this sequence version replaced XM_030255427.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.5080856.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC122421.4         53931-53959
            30-136              AC122421.4         80134-80240
            137-292             AC122421.4         81322-81477
            293-428             AC122421.4         82344-82479
            429-511             AC122421.4         96994-97076
            512-647             AC122421.4         97966-98101
            648-722             AC163104.2         28240-28314
            723-830             AC163104.2         34567-34674
            831-974             AC163104.2         38832-38975
            975-2520            AC163104.2         61617-63162
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 8.73 cM"
     gene            1..2520
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /note="tetraspanin 12"
                     /db_xref="GeneID:269831"
                     /db_xref="MGI:MGI:1889818"
     exon            1..29
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            30..136
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            137..292
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            293..428
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     CDS             363..1280
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /note="isoform 1 is encoded by transcript variant 3;
                     transmembrane 4 superfamily member 12"
                     /codon_start=1
                     /product="tetraspanin-12 isoform 1"
                     /protein_id="NP_001398676.1"
                     /db_xref="GeneID:269831"
                     /db_xref="MGI:MGI:1889818"
                     /translation="MAREDSVKCLRCLLYALNLLFWLMSISVLAVSAWMRDYLNNVLT
                     LTAETRVEEAVILTYFPVVHPVMIAVCCFLIIVGMLGYCGTVKRNLLLLAWYFGTLLV
                     IFCVELACGVWTYEQEVMVPVQWSDMVTLKARMTNYGLPRYRWLTHAWNYFQREFKCC
                     GVVYFTDWLEMTEMDWPPDSCCVREFPGCSKQAHQEDLSDLYQEGCGKKMYSFLRGTK
                     QLQVLRFLGISIGVTQILAMILTITLLWALYYDRREPGTDQMLSLKNDTSQHLSCHSV
                     ELLKPSLSRIFEHTSMANSFNTHFEMEEL"
     exon            429..511
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            512..647
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            648..722
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            723..830
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            831..974
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     exon            975..2520
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2498..2503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /note="hexamer: AATAAA"
     polyA_site      2520
                     /gene="Tspan12"
                     /gene_synonym="9030619E17; Tm4sf12"
                     /note="major polyA site"
ORIGIN      
        1 gcaagtgctg ggagaggcgc aagtggtttc agacaggtga tgatggatac agaccacagc
       61 tgcatgttgc cccgaagact ccgattatta gtttttctca aagatgttgc ttggatctaa
      121 ggtaacacaa tttttggagt tagcgacgcc gacggttgga gagaggaaga caaggaagcg
      181 tctgctgctg gcgccgatgc atccctttgg ctctccggga ggagcgtccc tgcccggatg
      241 agtgtctgct gtgtgtcccc gactgttccc cggcaggagc gcagctcgaa cgatcgccgg
      301 ggctctgcct ggaccagtag aatgtggtga gaggatgggg tttctccctc ccaagactca
      361 caatggccag agaagattcc gtgaagtgtt tgcgctgctt gctctacgcc ctcaacctgc
      421 tcttttggtt aatgtccatc agtgtcttgg cagtttctgc ttggatgagg gactacctga
      481 ataatgtttt gactttaact gcagaaacaa gggtagaaga agcggtcatc ttaacttact
      541 tccccgtggt tcaccccgtc atgattgctg tctgctgctt ccttatcatc gtgggaatgc
      601 tgggatactg tggaacggtg aaaagaaatc tgttgcttct tgcatggtac ttcggaacgt
      661 tactcgtcat cttctgtgta gaactggctt gcggtgtgtg gacatacgag caggaggtta
      721 tggtgccagt acagtggtca gatatggtta ctttgaaagc cagaatgaca aattacggct
      781 taccgaggta tagatggctt acacacgctt ggaattattt tcagagagag tttaagtgct
      841 gtggagtggt gtacttcact gactggttgg aaatgacaga aatggactgg cctcccgact
      901 cctgctgtgt cagggagttc ccagggtgct ccaagcaggc tcaccaggaa gatctcagtg
      961 acctttacca agagggttgt gggaagaaga tgtattcctt tttgagagga accaaacaat
     1021 tgcaagttct aaggtttctg ggaatctcca ttggtgtgac acaaatcctg gccatgattc
     1081 tcaccattac tctgctctgg gctctgtatt atgatagaag ggagcctgga acagaccaaa
     1141 tgctatctct gaaaaatgat acgtctcagc acttgtcatg tcattctgtg gaactgttaa
     1201 aaccaagtct ttcaaggatc tttgagcata catcaatggc aaacagcttc aatacacatt
     1261 ttgagatgga agaattatga agaatgtcaa agaaggaaat cacaaacttg tgggactagt
     1321 gggttttggg gtacacagtt atctatttca gaaattttta gacatattaa tattctgaaa
     1381 aggaatgtct aagaatttct ccactcttta aaacaaaggt tccacatggt aatacacaga
     1441 caataattgg tgcccttcaa aatgccgaac ccagacatta taacaactgt gtgtgctttc
     1501 tttcttttct ttttttctga gcacaattat gttttgcagt gatacagttt gaccagcatt
     1561 tctgcatcca ttcacagaaa tgaaccgtca tggtggcact ggagctgata tgaaggttaa
     1621 ctgactcctc taactaacct ataaactatc cactgactgc taacataggg atttagtgct
     1681 aaagaccttt attagctggt gatgtattgt tctgatggtt atttgattat atgcaaaaat
     1741 ctttcaagat tggtgactac ctaaatatga tttttgttgg ttactaaaat aatcttatta
     1801 ataacacttg taagagctaa catgttgtct taaacttatc agggatatat atgtatataa
     1861 gtctgtgccg tgtctgtata attcagtaga tttcagttcc tataatatta tgaaaaacaa
     1921 tatttgaaaa tggcaaaatt gtcttacaca gtaccattac ttttgtctct ttcagcaaca
     1981 gaacttttaa attccgtctt ggttctgtac tatatctata actgttaatt taagttgtta
     2041 accactaatt ttggaaatta ccagtttgac acataggaat ctctgtaagt ctggctgccg
     2101 gaagtattaa ctacagaatt tacacatagt tgattcatca aaagttcaga tttttccccc
     2161 ctcccaaaaa acactttgtc aaagagtgtt tttcgttttt atttttaatg ttttcatgta
     2221 tttcttttgc aatcaagaag caacaacttg aagttgatgt aattttacag agaaacaaca
     2281 ttctctgaag agatgctcat aggaatctct aaaataagtg gtagcctaac cagagaaaat
     2341 tcttgatttc tttcatcaat taatacactg tagcaaatga tgcagaatat taattttttt
     2401 acaagagtag aagatattta tttgaaacgg gaaaagtgca ttttactgta tcttgtgcat
     2461 tctgttcatt tcttggatta cagaaagaaa attaatcaat aaatattttc tagatcatga
//
