LOCUS       NM_001024619            2510 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tsukushi, small leucine rich proteoglycan (Tsku),
            transcript variant 3, mRNA.
ACCESSION   NM_001024619 XM_485965
VERSION     NM_001024619.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Istiaq,A., Umemoto,T., Ito,N., Suda,T., Shimamura,K. and Ohta,K.
  TITLE     Tsukushi proteoglycan maintains RNA splicing and developmental
            signaling network in GFAP-expressing subventricular zone neural
            stem/progenitor cells
  JOURNAL   Front Cell Dev Biol 10, 994588 (2022)
   PUBMED   36478736
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Wang,Q., Qiu,X., Liu,T., Ahn,C., Horowitz,J.F. and Lin,J.D.
  TITLE     The hepatokine TSK maintains myofiber integrity and exercise
            endurance and contributes to muscle regeneration
  JOURNAL   JCI Insight 7 (4), e154746 (2022)
   PUBMED   35025761
  REMARK    GeneRIF: The hepatokine TSK maintains myofiber integrity and
            exercise endurance and contributes to muscle regeneration.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2510)
  AUTHORS   Miwa,T., Ito,N. and Ohta,K.
  TITLE     Tsukushi is essential for the formation of the posterior
            semicircular canal that detects gait performance
  JOURNAL   J Cell Commun Signal 15 (4), 581-594 (2021)
   PUBMED   34061311
REFERENCE   4  (bases 1 to 2510)
  AUTHORS   Wang,Q., Zhang,P., Cakir,I., Mi,L., Cone,R.D. and Lin,J.D.
  TITLE     Deletion of the Feeding-Induced Hepatokine TSK Ameliorates the
            Melanocortin Obesity Syndrome
  JOURNAL   Diabetes 70 (9), 2081-2091 (2021)
   PUBMED   34183373
  REMARK    GeneRIF: Deletion of the Feeding-Induced Hepatokine TSK Ameliorates
            the Melanocortin Obesity Syndrome.
REFERENCE   5  (bases 1 to 2510)
  AUTHORS   Ito,N., Riyadh,M.A., Ahmad,S.A.I., Hattori,S., Kanemura,Y.,
            Kiyonari,H., Abe,T., Furuta,Y., Shinmyo,Y., Kaneko,N., Hirota,Y.,
            Lupo,G., Hatakeyama,J., Abdulhaleem,M. FA, Anam,M.B., Yamaguchi,M.,
            Takeo,T., Takebayashi,H., Takebayashi,M., Oike,Y., Nakagata,N.,
            Shimamura,K., Holtzman,M.J., Takahashi,Y., Guillemot,F.,
            Miyakawa,T., Sawamoto,K. and Ohta,K.
  TITLE     Dysfunction of the proteoglycan Tsukushi causes hydrocephalus
            through altered neurogenesis in the subventricular zone in mice
  JOURNAL   Sci Transl Med 13 (587) (2021)
   PUBMED   33790026
  REMARK    GeneRIF: Dysfunction of the proteoglycan Tsukushi causes
            hydrocephalus through altered neurogenesis in the subventricular
            zone in mice.
REFERENCE   6  (bases 1 to 2510)
  AUTHORS   Niimori,D., Kawano,R., Felemban,A., Niimori-Kita,K., Tanaka,H.,
            Ihn,H. and Ohta,K.
  TITLE     Tsukushi controls the hair cycle by regulating TGF-beta1 signaling
  JOURNAL   Dev Biol 372 (1), 81-87 (2012)
   PUBMED   22995554
  REMARK    GeneRIF: TSK controls the hair cycle by regulating TGF-beta1
            signaling.
REFERENCE   7  (bases 1 to 2510)
  AUTHORS   Ohta,K., Ito,A., Kuriyama,S., Lupo,G., Kosaka,M., Ohnuma,S.,
            Nakagawa,S. and Tanaka,H.
  TITLE     Tsukushi functions as a Wnt signaling inhibitor by competing with
            Wnt2b for binding to transmembrane protein Frizzled4
  JOURNAL   Proc Natl Acad Sci U S A 108 (36), 14962-14967 (2011)
   PUBMED   21856951
  REMARK    GeneRIF: targeted inactivation of the TSK gene in mice causes
            expansion of the ciliary body and up-regulation of Wnt2b and Fzd4
            expression in the developing peripheral eye
REFERENCE   8  (bases 1 to 2510)
  AUTHORS   Ito,A., Shinmyo,Y., Abe,T., Oshima,N., Tanaka,H. and Ohta,K.
  TITLE     Tsukushi is required for anterior commissure formation in mouse
            brain
  JOURNAL   Biochem Biophys Res Commun 402 (4), 813-818 (2010)
   PUBMED   21055390
  REMARK    GeneRIF: a crucial role for TSK for anterior commissure formation
            in the mouse brain was shown.
REFERENCE   9  (bases 1 to 2510)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   10 (bases 1 to 2510)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK137208.1 and AC111084.12.
            
            On Dec 10, 2009 this sequence version replaced NM_001024619.2.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. All four variants encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035461.1, AK137208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1620              AK137208.1         2-1621
            1621-2510           AC111084.12        115633-116522       c
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.66 cM"
     gene            1..2510
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /note="tsukushi, small leucine rich proteoglycan"
                     /db_xref="GeneID:244152"
                     /db_xref="MGI:MGI:2443855"
     exon            1..47
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            48..2510
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..1120
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /note="leucine-rich repeat-containing protein 54; leucine
                     rich repeat containing 54; tsukushin"
                     /codon_start=1
                     /product="tsukushi precursor"
                     /protein_id="NP_001019790.1"
                     /db_xref="CCDS:CCDS21469.1"
                     /db_xref="GeneID:244152"
                     /db_xref="MGI:MGI:2443855"
                     /translation="MLCSLFLLLLAVGRVQTTRPCFPGCQCEEETFGLFDSFSLIRVD
                     CSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLAGPGYTTLAGLDLSYNLLTSIMP
                     SAFSRLRYLESLDLSHNGLAALPAEIFTSSPLSDINLSHNRLREVSISAFTTHSQGRA
                     LHVDLSHNLIHRLLPHPARASLPAPTIQSLNLSWNRFRAVPDLRDLPLRYLSLDGNPL
                     ATINPDAFMGLAGLTHLSLASLQGILHLPPHGFRELPGLQVLDLSGNPKLKWAGAEVF
                     SGLGLLQELDLSGSSLVPLPEMLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSP
                     KVVLHCGDTQESAARGPDIL"
     regulatory      2476..2481
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /note="hexamer: AATAAA"
     polyA_site      2510
                     /gene="Tsku"
                     /gene_synonym="9530051K01Rik; E2ig4; Lrrc54; Tsk"
                     /note="major polyA site"
ORIGIN      
        1 gggtcgccta ggtgagcgtg ccgaggcgac aactgcagac ctgccaggtg tcaccatgct
       61 gtgctctctg ttcctgctgc tgctggccgt gggcagagtg cagacgactc ggccgtgttt
      121 ccctggctgc cagtgtgaag aagagacatt cggcctcttt gacagtttca gcctgatccg
      181 tgtggactgc agcagcctgg gcccccacat tgtgcctgtg cccatccctt tggatacagc
      241 ccacctggac ctgtcttcca accggctaga aaccgtgaat gagtcagtct tggcagggcc
      301 aggctatacc acactggctg gcctggatct cagttacaac ctgctcacca gcatcatgcc
      361 ctctgccttc tcccgactgc gctacctgga gtcacttgac ctcagccaca atggcctggc
      421 agccctgccg gcagagattt tcaccagctc ccccttgagt gacatcaatc tgagccataa
      481 ccgactacga gaggtctcga tatctgcctt caccacccac agccagggcc gggcactgca
      541 cgtggaccta tcccacaatc ttatccatcg cctgcttccc catccagccc gggccagcct
      601 gcctgcacct accattcaga gcctgaacct gtcctggaac cgattccgag ctgtgcccga
      661 tctccgagac ctacccctgc gttacctgag cctggatggg aaccctctgg ctaccatcaa
      721 cccagatgcc ttcatggggc tggcaggcct cacccacctg tcactggcca gcctgcaggg
      781 catcctccat ctaccacccc acggcttccg ggagctccca ggccttcagg tcctggactt
      841 gtcaggcaac cccaagctca agtgggcagg agctgaggtg ttttcaggcc tgggtttgct
      901 gcaggaacta gacctgtcag gctccagctt ggtgcccctg cctgagatgc tgctgcatca
      961 cctccctgct ttacaaagtg tcagcgtagg ccaggatgtg cagtgccggc gcctggtgcg
     1021 ggagggcgcc taccaccggc agcctggctc cagccctaag gtagtcctac actgtggaga
     1081 cacccaggaa tctgctgcca ggggcccaga cattttgtga cgaatggtgt ggcctgaggc
     1141 cacttgacag actgctgcct gggcttgctc aggtccccac taatatttta atgtgccaat
     1201 gcctgtggag agtctacagg cctaagtggc ggccatctca gtaaagatat gggccaagga
     1261 tagtttttgt acctggggcc tatacccaat aacaaaatct gccggtttgt cacagtggct
     1321 cacctcccag aactctgagc aaagggactg tgatgccaaa ccagactttg gtcccttcct
     1381 gagccctgct ccagttgtcc ctaagtgcct tcccttttgt ctggactgac ctgacccatg
     1441 atgtttggac tacatgtagg tgagcgcccg cattgcaaag ctgactcagg tccactgggc
     1501 tgaaggtccc ttggagccca gtgtctagtc acaccttttt gctctgggcc catgaggcct
     1561 acttcttcct tttctcatgg aatcctgggt agaatcttaa taatagaagg acttagagag
     1621 aaaatcaaat ccaccctcct tgtggggaag atggtaatat gggaccaaga gcagcaaacg
     1681 tgctcacctg gtgtcttgga gcccagcctc ctgctgaggt tgggggctct gccatccctc
     1741 ccctgctggg taccctgtta ccattcattg tctgtgctgt ttcccattgt gcccagcagt
     1801 agcaacaaca agatcacatg ggtggggacc ctaggtccgg tggccagctt atggcaggag
     1861 caaagtcagt ccccctttca gagcctcagg aagcttggaa cacctcagtc acaagcctgt
     1921 cagtttcttc ctctgggatg ggagtcccgc agcatcgaga ctggaaacgt acacatttcc
     1981 cccagggacc ctgtcttacg atgcttttct acgtcaatgc aattgtggcc atctggccac
     2041 aagctccaag ggacttacaa gctcaagggc cagagcccag agacccattt tcccctccaa
     2101 ggagtggggc aggaagggtc tcccaggcac gctgtgctca cccccagggc cacatctcag
     2161 ctctccagcc ctggattctt tcctgtcttc attttatgag ttgttgccct tttttttttt
     2221 tttttttttt ttttaatgaa ctgtcacttt taaccccctc ctctgctggc cgggggatgg
     2281 aaaggaagat aaatgttatt tgttcacttt tgtaagactc tgttctgggt gtgggccagg
     2341 aggtcttaga aagaagctgg ctgtcagtga ccatgtgggc attgaggctg gtcccagaga
     2401 tgctcgaaag tcagtgaaag ctatgccttc tcatgctagc ccagcccagc ccaccctgcg
     2461 actggttctt ggtttaataa acacatgtaa gagttctttc caccactgaa
//
