LOCUS       NM_011651               1828 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus testis-specific serine kinase substrate (Tsks),
            transcript variant 1, mRNA.
ACCESSION   NM_011651 XM_893754
VERSION     NM_011651.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Shimada,K., Park,S., Oura,S., Noda,T., Morohoshi,A., Matzuk,M.M.
            and Ikawa,M.
  TITLE     TSKS localizes to nuage in spermatids and regulates cytoplasmic
            elimination during spermiation
  JOURNAL   Proc Natl Acad Sci U S A 120 (11), e2221762120 (2023)
   PUBMED   36881620
  REMARK    GeneRIF: TSKS localizes to nuage in spermatids and regulates
            cytoplasmic elimination during spermiation.
REFERENCE   3  (bases 1 to 1828)
  AUTHORS   MacLeod,G., Shang,P., Booth,G.T., Mastropaolo,L.A.,
            Manafpoursakha,N., Vogl,A.W. and Varmuza,S.
  TITLE     PPP1CC2 can form a kinase/phosphatase complex with the
            testis-specific proteins TSSK1 and TSKS in the mouse testis
  JOURNAL   Reproduction 147 (1), 1-12 (2013)
   PUBMED   24088291
  REMARK    GeneRIF: In mouse testis, PPP1CC2 can form a complex with TSSK1
            mediated by the direct interaction of each with the kinase
            substrate protein TSKS. Interaction between PPP1CC2 and TSKS is
            mediated through an RVxF docking motif on the TSKS surface.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1828)
  AUTHORS   Shang,P., Baarends,W.M., Hoogerbrugge,J., Ooms,M.P., van
            Cappellen,W.A., de Jong,A.A., Dohle,G.R., van Eenennaam,H.,
            Gossen,J.A. and Grootegoed,J.A.
  TITLE     Functional transformation of the chromatoid body in mouse
            spermatids requires testis-specific serine/threonine kinases
  JOURNAL   J Cell Sci 123 (Pt 3), 331-339 (2010)
   PUBMED   20053632
  REMARK    GeneRIF: In elongating spermatids, data detected accumulation of
            the testis-specific TSSK1 and TSSK2, and the substrate TSKS, in a
            ring-shaped structure around the base of the flagellum and in a
            cytoplasmic satellite.
REFERENCE   5  (bases 1 to 1828)
  AUTHORS   Xu,B., Hao,Z., Jha,K.N., Zhang,Z., Urekar,C., Digilio,L.,
            Pulido,S., Strauss,J.F. 3rd, Flickinger,C.J. and Herr,J.C.
  TITLE     Targeted deletion of Tssk1 and 2 causes male infertility due to
            haploinsufficiency
  JOURNAL   Dev Biol 319 (2), 211-222 (2008)
   PUBMED   18533145
REFERENCE   6  (bases 1 to 1828)
  AUTHORS   Xu,B., Hao,Z., Jha,K.N., Zhang,Z., Urekar,C., Digilio,L.,
            Pulido,S., Strauss,J.F. 3rd, Flickinger,C.J. and Herr,J.C.
  TITLE     TSKS concentrates in spermatid centrioles during flagellogenesis
  JOURNAL   Dev Biol 319 (2), 201-210 (2008)
   PUBMED   18495105
REFERENCE   7  (bases 1 to 1828)
  AUTHORS   Kueng,P., Nikolova,Z., Djonov,V., Hemphill,A., Rohrbach,V.,
            Boehlen,D., Zuercher,G., Andres,A.C. and Ziemiecki,A.
  TITLE     A novel family of serine/threonine kinases participating in
            spermiogenesis
  JOURNAL   J Cell Biol 139 (7), 1851-1859 (1997)
   PUBMED   9412477
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ047818.1, CN831975.1,
            BC121827.1 and BX630702.1.
            
            On Nov 13, 2006 this sequence version replaced NM_011651.1.
            
            Summary: This gene is a member of a small family of testis-specific
            serine/threonine kinases. This gene may play a role in testicular
            physiology or spermiogenesis. Its expression is restricted to the
            testis during the last stages of spermatid maturation. Alternative
            splicing results in multiple transcript variants encoding distinct
            proteins. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer protein (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF025310.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-279               CJ047818.1         2-280
            280-786             CN831975.1         3-509
            787-1645            BC121827.1         739-1597
            1646-1828           BX630702.1         1-183               c
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.07 cM"
     gene            1..1828
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /note="testis-specific serine kinase substrate"
                     /db_xref="GeneID:22116"
                     /db_xref="MGI:MGI:1347560"
     exon            1..214
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..1802
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /note="isoform 1 is encoded by transcript variant 1; STK22
                     substrate 1; testis-specific kinase substrate;
                     serine/threonine kinase 22 substrate 1; testis-specific
                     serine/threonine kinase substrate 1; clone 4"
                     /codon_start=1
                     /product="testis-specific serine kinase substrate isoform
                     1"
                     /protein_id="NP_035781.2"
                     /db_xref="CCDS:CCDS52240.1"
                     /db_xref="GeneID:22116"
                     /db_xref="MGI:MGI:1347560"
                     /translation="MASVVVKTIWQSKEIHEAGDPPAGVESRAQLVPEAPGGVTSPAK
                     GITKKKKAVSFHGVEPRMSHEPMHWCLNLKRSSACTNVSLLNLAAVEPDSSGTDSTTE
                     DSGPLALPGPPASPTTPWAPEDPDITELLSGVNSGLVRAKDSITSLKEKTTRVNQHVQ
                     TLQSECSVLSENLERRRQEAEELEGYCSQLKGPRPDVLTQENCRKVTRSVEDAEIKTN
                     VLKQNSALLEEKLRYLQQQLQDETPRRQEAELQELEQKLEAGLSRHGLSPATPIQGCS
                     GPPGSPEEPPRQRGLSSSGWGMAVRTGEGPSLSEQELQKVSTGLEELRREVSSLAARW
                     HQEEGAVQEALRLLGGLGGRLDGFLGQWERAQREQAQSARGLQELRGRADELCTMVER
                     SAVSVASLRSELEALGPVKPILEELGRQLQNSRRGADHVLNLDRSAQGPCARCASQGQ
                     QLSTESLQQLLERALTPLVDEVKQKGLAPACPSCQRLHKKILELERQALAKHVRAEAL
                     SSTLRLAQDEAVRAKNLLLTDKMKPEEKVATLDYMHLKMCSLHDQLSHLPLEGSTGAM
                     GGGSNGGAPPKRGSPGSEQ"
     exon            215..437
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            438..533
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            534..617
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            618..728
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            729..1015
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1210
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1384
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1520
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1645
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1825
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1798..1803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /note="hexamer: AATAAA"
     polyA_site      1825
                     /gene="Tsks"
                     /gene_synonym="Stk22s1; Tssks1"
                     /note="major polyA site"
ORIGIN      
        1 agagtagatg gtgagtctcc ccctgggagc aggccccccg caccatggca agcgtggtgg
       61 tgaagacaat ctggcaatcc aaagagatcc acgaagcggg ggacccacct gcgggggtag
      121 aaagccgtgc ccagctggtc cccgaggctc ccgggggggt gaccagccct gccaaaggga
      181 taacgaaaaa aaagaaggct gtgtccttcc atggggtgga gccccggatg tcccacgagc
      241 cgatgcactg gtgcctgaac ctcaagcggt cctctgcctg caccaacgtg tccttgctca
      301 acctggctgc cgtggagccc gactcctcgg gcacagactc gaccacagag gacagtggtc
      361 cactggcact gccagggcca cctgcttccc ctacaacacc ctgggctcca gaggaccctg
      421 acatcacaga actactgagt ggggtcaaca gtggattggt acgtgccaaa gactccatca
      481 ccagcttgaa ggaaaagacc acgcgggtta atcagcacgt tcagactctg cagagcgagt
      541 gctctgtgct gagtgagaat ctggaaagaa gacggcagga ggcagaagag ttggaggggt
      601 actgcagtca gttgaagggc ccccgccctg atgtcctgac ccaggagaac tgccgcaagg
      661 tgacccgttc agtggaagac gctgaaatca aaaccaatgt cctgaagcag aactctgcct
      721 tgctggagga gaagctaaga tacctccagc agcaactgca ggatgagacg ccccggagac
      781 aggaggccga gttgcaggag ttggagcaaa aactggaggc tggcctctcc cgacatggtc
      841 tgagccctgc cactcccatt cagggctgct cgggtcctcc tggcagcccc gaggaacccc
      901 cgcggcagcg aggcctgtcc tccagtggct ggggcatggc agtccgcaca ggggagggac
      961 cctcgctgag cgagcaggag ttgcagaagg tctccaccgg cctggaggag ctgaggaggg
     1021 aggtgtcctc gctggcagcc cggtggcatc aggaggaggg ggcagtgcag gaggccctga
     1081 ggttgctggg aggccttggc ggccgtctgg atggcttcct gggccagtgg gagcgggcgc
     1141 agcgggaaca ggcacagtcc gcaaggggct tgcaggagct gcgaggacga gcagatgagt
     1201 tgtgcactat ggtggagagg tcagcagtgt ctgtcgcctc actgaggagt gaactggagg
     1261 cactgggccc agtaaaaccc attctggagg agctgggacg gcaacttcag aactcccgga
     1321 ggggagctga ccatgtcttg aacttggatc ggtctgccca aggcccctgt gcgcgctgtg
     1381 ccagccaggg gcagcagtta tccacggagt ccctgcagca gctgctggaa cgtgcgctga
     1441 ccccgctggt ggatgaggtg aagcagaagg gtctggctcc tgcctgcccc agttgccaga
     1501 ggctacacaa gaagattctg gagctggagc gccaggcctt ggccaaacac gtcagggcag
     1561 aggccctgag ctccaccctt cggttggccc aagacgaggc cgttcgggcc aagaacctac
     1621 tgctgacgga caagatgaag ccggaggaga aggtggccac tttggactat atgcatttga
     1681 agatgtgctc cctccacgac caactcagcc acctgccact tgaggggtcc acgggggcaa
     1741 tggggggagg aagcaatgga ggggctcccc ctaaacgtgg gagtccaggc tctgaacaat
     1801 aaatggcctc tcatgctggc atgaaaaa
//
