LOCUS       NM_021898               1074 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus testis specific gene A8 (Tsga8), mRNA.
ACCESSION   NM_021898 XM_003085273 XM_912227
VERSION     NM_021898.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Kobayashi,Y., Tomizawa,S.I., Ono,M., Kuroha,K., Minamizawa,K.,
            Natsume,K., Dizdarevic,S., Dockal,I., Tanaka,H., Kawagoe,T.,
            Seki,M., Suzuki,Y., Ogonuki,N., Inoue,K., Matoba,S.,
            Anastassiadis,K., Mizuki,N., Ogura,A. and Ohbo,K.
  TITLE     Tsga8 is required for spermatid morphogenesis and male fertility in
            mice
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33766931
  REMARK    GeneRIF: Tsga8 is required for spermatid morphogenesis and male
            fertility in mice.
REFERENCE   3  (bases 1 to 1074)
  AUTHORS   Lu,Y., Liao,S., Tu,W., Yang,B., Liu,S., Pei,X., Tao,D., Lu,Y.,
            Ma,Y., Yang,Y. and Liu,Y.
  TITLE     DNA demethylation facilitates the specific transcription of the
            mouse X-linked Tsga8 gene in round spermatidsdagger
  JOURNAL   Biol Reprod 100 (4), 994-1007 (2019)
   PUBMED   30541061
  REMARK    GeneRIF: Details of a regulatory mechanism that includes DNA
            methylation and transcription factors to mediate the postmeiotic
            expression of an X-linked gene, Tsga8.
REFERENCE   4  (bases 1 to 1074)
  AUTHORS   Stouffs,K., Tournaye,H., Liebaers,I. and Lissens,W.
  TITLE     Male infertility and the involvement of the X chromosome
  JOURNAL   Hum Reprod Update 15 (6), 623-637 (2009)
   PUBMED   19515807
  REMARK    Review article
REFERENCE   5  (bases 1 to 1074)
  AUTHORS   Oka,A., Mita,A., Sakurai-Yamatani,N., Yamamoto,H., Takagi,N.,
            Takano-Shimizu,T., Toshimori,K., Moriwaki,K. and Shiroishi,T.
  TITLE     Hybrid breakdown caused by substitution of the X chromosome between
            two mouse subspecies
  JOURNAL   Genetics 166 (2), 913-924 (2004)
   PUBMED   15020476
REFERENCE   6  (bases 1 to 1074)
  AUTHORS   Uchida,K., Tsuchida,J., Tanaka,H., Koga,M., Nishina,Y., Nozaki,M.,
            Yoshinaga,K., Toshimori,K., Matsumiya,K., Okuyama,A. and
            Nishimune,Y.
  TITLE     Cloning and characterization of a complementary deoxyribonucleic
            acid encoding haploid-specific alanine-rich acidic protein located
            on chromosome-X
  JOURNAL   Biol Reprod 63 (4), 993-999 (2000)
   PUBMED   10993819
REFERENCE   7  (bases 1 to 1074)
  AUTHORS   Taketo,M.M., Araki,Y., Matsunaga,A., Yokoi,A., Tsuchida,J.,
            Nishina,Y., Nozaki,M., Tanaka,H., Koga,M., Uchida,K., Matsumiya,K.,
            Okuyama,A., Rochelle,J.M., Nishimune,Y., Matsui,M. and Seldin,M.F.
  TITLE     Mapping of eight testis-specific genes to mouse chromosomes
  JOURNAL   Genomics 46 (1), 138-142 (1997)
   PUBMED   9403069
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB032764.1 and CR382328.14.
            
            On or before Jan 17, 2011 this sequence version replaced
            XM_003085273.1, NM_021898.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032764.1, AB046267.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AB032764.1         3-19
            18-1070             AB032764.1         21-1073
            1071-1074           CR382328.14        122531-122534       c
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 38.38 cM"
     gene            1..1074
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /note="testis specific gene A8"
                     /db_xref="GeneID:100502723"
                     /db_xref="MGI:MGI:1194903"
     exon            1..354
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..963
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /note="haploid specific alanine rich acidic protein, X
                     chromosome; testis-specific protein A8; haploid-specific
                     alanine-rich acidic protein located on chromosome X"
                     /codon_start=1
                     /product="testis-specific gene A8 protein"
                     /protein_id="NP_068698.1"
                     /db_xref="CCDS:CCDS53124.1"
                     /db_xref="GeneID:100502723"
                     /db_xref="MGI:MGI:1194903"
                     /translation="MDVPTSSTDSQEVKPIMPIRTKAACKFYPPHILCGKGAKTNKRG
                     KRGGAQKASTKKDIGETTPPVAKKSKVAKEPTMLAAAAAPEAAASPESSAAAAAPEAA
                     ASPESSAAAAAPEAAASPESSAAAAAPEAAASLESSAAAAAPEAAAAPATPAAPEAAA
                     APEVAAAPATPAAPEATAAPAAPEAATTPAAPEAPAAAPAVEEEEMVWEAAAVVGEAA
                     VKPPEEEPTSGEAVATTTMT"
     exon            355..1074
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1051..1056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /note="hexamer: AATAAA"
     polyA_site      1074
                     /gene="Tsga8"
                     /gene_synonym="Halap-X; Halapx"
                     /note="major polyA site"
ORIGIN      
        1 ggtacattct gctccttcac caggcacgct cgtgcatcat agccatgctt tgtgacggct
       61 gagcacgtgc tcgcctacta ttggctgagg acctggtgac gaaccagact ttatgggtgt
      121 ggccttcaat gctctggcag ttgctcccga gctctcgagg tagcaacact tgaaagctgt
      181 tggcggcgag ggttactaag aaatcaaagg atttcgtgta agaaatcaaa ggatttcgga
      241 tcggaaatgg atgttccgac ttcatccacc gattctcagg aggtgaagcc tataatgcca
      301 attaggacca aggcagcctg caagttttat cctcctcaca ttctttgtgg aaagggtgcg
      361 aagaccaaca agcgtgggaa acgcggtggc gcccaaaagg cttccacaaa gaaagacatc
      421 ggagaaacta ctccacctgt ggctaagaag tccaaagtgg ccaaggagcc aaccatgctg
      481 gcggcagcag ccgcccctga ggcagcagcc tcacctgagt catcagccgc agcagccgcc
      541 cctgaggcag cagcctcacc tgagtcatca gccgcagcag ccgcacctga ggcagcagcc
      601 tcacctgagt catcagcggc agcagccgca cctgaggcag cagcctcact tgagtcatca
      661 gcggcagcag ccgcacctga ggcagcagcc gcacctgcta cacctgctgc acctgaggca
      721 gcagccgcac ctgaggtagc agccgcacct gctacacctg ccgcacctga ggcaacagct
      781 gcacctgccg cacctgaggc agcaactaca cctgctgcac ctgaggcacc agcagctgct
      841 ccagctgtag aggaggaaga aatggtctgg gaggcggcag cagtcgttgg ggaggcagct
      901 gtgaagcccc ccgaggagga accgaccagc ggagaagcag tcgctactac aaccatgacc
      961 taggcagata gccaataaga tggctatctc agtgctggga attccccaga accccagccc
     1021 ccaaataaaa attaacatat tgatgttaaa aataaactga ttaaaatttg aaaa
//
