LOCUS       NM_001290721            2723 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus testis specific 10 (Tsga10), transcript variant 3,
            mRNA.
ACCESSION   NM_001290721
VERSION     NM_001290721.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Luo,G., Hou,M., Wang,B., Liu,Z., Liu,W., Han,T., Zhang,D., Zhou,X.,
            Jia,W., Tan,Y., Wu,Y., Wang,J. and Zhang,X.
  TITLE     Tsga10 is essential for arrangement of mitochondrial sheath and
            male fertility in mice
  JOURNAL   Andrology 9 (1), 368-375 (2021)
   PUBMED   32790169
  REMARK    GeneRIF: Tsga10 is essential for arrangement of mitochondrial
            sheath and male fertility in mice.
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Tajaddini Mahani,S., Behnam,B., Abbassi,M., Asgari,H., Nazmara,Z.,
            Shirinbayan,P., Joghataei,M.T. and Koruji,M.
  TITLE     Tsga10 expression correlates with sperm profiles in the adult
            formalin-exposed mice
  JOURNAL   Andrologia 48 (10), 1092-1099 (2016)
   PUBMED   26791599
  REMARK    GeneRIF: This study also shows that Tsga10 expression protects
            sperm dysfunction in formalin plus manganese-exposed mice resulting
            in better preservation of spermatozoa and improvement of male
            fertility.
REFERENCE   3  (bases 1 to 2723)
  AUTHORS   Hagele,S., Behnam,B., Borter,E., Wolfe,J., Paasch,U., Lukashev,D.,
            Sitkovsky,M., Wenger,R.H. and Katschinski,D.M.
  TITLE     TSGA10 prevents nuclear localization of the hypoxia-inducible
            factor (HIF)-1alpha
  JOURNAL   FEBS Lett 580 (15), 3731-3738 (2006)
   PUBMED   16777103
  REMARK    GeneRIF: HIF-1alpha nuclear localization and HIF-1 transcriptional
            activity were significantly affected by overexpressed TSGA10
REFERENCE   4  (bases 1 to 2723)
  AUTHORS   Behnam,B., Modarressi,M.H., Conti,V., Taylor,K.E., Puliti,A. and
            Wolfe,J.
  TITLE     Expression of Tsga10 sperm tail protein in embryogenesis and neural
            development: from cilium to cell division
  JOURNAL   Biochem Biophys Res Commun 344 (4), 1102-1110 (2006)
   PUBMED   16643851
  REMARK    GeneRIF: Tsga10 expression starts in 4.5-7.5 dpc mouse embryos and
            continues throughout embryogenesis.
REFERENCE   5  (bases 1 to 2723)
  AUTHORS   Modarressi,M.H., Behnam,B., Cheng,M., Taylor,K.E., Wolfe,J. and van
            der Hoorn,F.A.
  TITLE     Tsga10 encodes a 65-kilodalton protein that is processed to the
            27-kilodalton fibrous sheath protein
  JOURNAL   Biol Reprod 70 (3), 608-615 (2004)
   PUBMED   14585816
  REMARK    GeneRIF: Tsga10 plays a role in the sperm tail fibrous sheath.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ046680.1, AK030254.1, BC053407.1, AC123068.4, CK789086.1 and
            BX637247.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and has
            an alternate 3' structure and 3' terminal exon, compared to variant
            1. The encoded protein (isoform 3) has a shorter and distinct
            C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF530050.3, SRR7974084.3373.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-385               CJ046680.1         57-441
            386-1114            AK030254.1         579-1307
            1115-1255           BC053407.1         1098-1238
            1256-1478           AK030254.1         1449-1671
            1479-1479           AC123068.4         173528-173528       c
            1480-1932           AK030254.1         1673-2125
            1933-2097           CK789086.1         502-666
            2098-2142           AC123068.4         150252-150296       c
            2143-2597           AK030254.1         2126-2580
            2598-2718           AK030254.1         2581-2701
            2719-2723           BX637247.1         3-7                 c
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 15.88 cM"
     gene            1..2723
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /note="testis specific 10"
                     /db_xref="GeneID:211484"
                     /db_xref="MGI:MGI:2685063"
     exon            1..129
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            130..345
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            346..411
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            412..579
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     CDS             529..2601
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /note="isoform 3 is encoded by transcript variant 3;
                     testis-specific gene 10 protein"
                     /codon_start=1
                     /product="testis-specific gene 10 protein isoform 3"
                     /protein_id="NP_001277650.1"
                     /db_xref="CCDS:CCDS78571.1"
                     /db_xref="GeneID:211484"
                     /db_xref="MGI:MGI:2685063"
                     /translation="MMRNRSKSPRRPSPTSRAANCDVELLKSTARDREELKCMLEKYE
                     RHLAEIQGNVKVLTSERDKTFLLYEQAQEEIARLRREMMKSCKSPKSTTAHAILRRVE
                     TERDVAFTDLRRMTTERDSLRERLKIAQETAFNEKAHLEQRIEELECTVHNLDDERME
                     QMANMTLMKETITTVEKEMKSLARKAMDTESELGRQKAENNSLRLLYENTEKDLSDTQ
                     RHLAKKKYELQLTQEKIMCLDEKIDNFTRQNIAQREEISILGATLNDLAKEKECLQAC
                     LDKKSENIASLGESLAMKEKTISGMKNIIAEMEQASRQSTEALIMCEQDISRMRRQLD
                     ETNDELGQIARERDILAHENDNLQEQFAKVKQENQALSKKLNDTHNELSDIKQKVQDT
                     NLEVNKLKNILKSEESENRQIMEQLRKANEDAENWENKARQTEAENNTLKLELITAEA
                     EGNRLKEKVDALNREVEQHLNAERSYKSQIATLHKSLVKMEEELQKVQFEKVSALADL
                     SSTRELCIKLDSSKELLNRQLVAKDQEIEMMENELDSARSEIELLRSQMTNERISMQN
                     LEALLVANRDKEYQSQIALQEKESEIQLLKEHLCLAENKMAIQSRDVAQFRNVVTQLE
                     ADLDITKRQLGTERFERERAVQELRRQNYSSNAYNLGPMKPNTKCHSPERAHHRSPDR
                     GLDRSLEE"
     exon            580..738
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            739..909
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            910..987
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1139
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1255
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1410
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1466
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1635
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1746
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1932
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2142
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2345
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2450
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2723
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2701..2706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /note="hexamer: AATAAA"
     polyA_site      2723
                     /gene="Tsga10"
                     /gene_synonym="4933432N21Rik; Gm217; Mtsga10"
                     /note="major polyA site"
ORIGIN      
        1 gttaacacca aagaacatca gtttggtctt ggggctccag tttctgaagc tggaagaaac
       61 cagaatactt tccaactaga acaagaagtg agaacccaag atagattcat ctctacactg
      121 aaattacaga ttgaagatct caaacagaca aatcatgact tggaagaata tgttaggaaa
      181 ctcttggata gtaaagaggc ggtaagcact caagtagatg acttagccaa ccacaatgag
      241 cacctttgta aagagttgat taaacttgac caactagcag agaaattaca aaaagaaaaa
      301 aattttgtgg tggacactgc cgacaaggaa cttgaagaag caaagattga actcatttgc
      361 cagcaaaata atataacagt attagaagat acaatccaaa ggcttaagtc cataatttta
      421 gagactgaaa aggcacaaaa tacatctcct tctagactgg attcctttgt caagacttta
      481 gaagcagaca gagattatta taagactgaa gctcagaatt taagaaaaat gatgagaaat
      541 agatctaaga gtccgagacg cccatcgcca acttcccggg ctgcaaactg tgatgtagag
      601 cttttgaagt caactgcaag agaccgtgaa gagctaaagt gtatgctgga aaaatatgaa
      661 cgtcatttgg cagaaatcca gggcaacgtc aaggttctca catctgagag agacaagacc
      721 ttcctccttt atgagcaggc acaggaagaa attgctcgac ttcgacgaga aatgatgaaa
      781 agctgtaagt ctcctaagtc aaccacagca catgctattc ttcgtcgggt agagacggag
      841 agagatgtag ccttcactga cctacgaaga atgaccacag agcgagacag tctgagagaa
      901 aggctcaaga ttgctcaaga gacagcgttc aacgagaagg ctcacttgga acagcggata
      961 gaggagctgg agtgcacagt tcacaacctt gatgatgagc gcatggaaca gatggcaaac
     1021 atgactttga tgaaggaaac cataaccact gtggaaaaag aaatgaaatc attagcaaga
     1081 aaggcaatgg acaccgagag tgagcttggc agacaaaaag cagagaataa ttctttgaga
     1141 cttttatatg aaaacacaga aaaagatctc tctgatactc agcgacatct tgctaagaaa
     1201 aaatatgagc tacagcttac tcaggagaaa attatgtgct tggatgaaaa aattgataat
     1261 ttcacgaggc aaaatattgc acagcgagaa gaaatcagca ttcttggtgc aaccctcaat
     1321 gacctggcta aagaaaagga atgcctgcaa gcgtgtctgg ataaaaagtc tgagaacatt
     1381 gcatcccttg gcgagagctt ggcaatgaaa gaaaaaacca tttcaggcat gaagaatatc
     1441 attgctgaga tggaacaggc atcaagacag tctactgaag ccctcattat gtgcgaacaa
     1501 gatatttcca ggatgcgccg gcagttggac gagacaaatg atgagctggg tcagattgcc
     1561 agggagagag atatcttggc tcatgagaat gacaatcttc aagaacagtt tgccaaagtc
     1621 aaacaagaaa accaggcact gtccaaaaaa ctgaatgata ctcataacga actcagtgac
     1681 ataaagcaga aggtccagga cacgaatctg gaggttaaca agttgaagaa catattaaag
     1741 tctgaggaat ctgagaaccg gcaaataatg gaacaactcc gaaaagccaa tgaagatgct
     1801 gaaaactggg aaaataaggc ccgccagaca gaggcagaaa acaacaccct caaactggaa
     1861 cttatcactg ctgaagcaga gggcaacaga ttaaaggaaa aagttgatgc ccttaacaga
     1921 gaggttgagc aacacctaaa cgcagagcgc tcttacaaat cccagattgc aactttacac
     1981 aagtctcttg tgaagatgga ggaggagctt cagaaggttc agtttgagaa ggtgtctgct
     2041 ctcgcagatt tgtcttccac aagggaactc tgcataaaac tcgactcaag caaagaactt
     2101 cttaatcgac agctggttgc caaagatcag gaaatagaaa tgatggagaa tgagctggac
     2161 tcagcgcgct ctgaaattga actgctccgg agtcagatga caaacgagag gatctccatg
     2221 cagaatctcg aggctctgct ggtggccaac cgggacaaag agtaccagtc tcagatagca
     2281 ctgcaggaaa aggagtctga gatccagttg ctgaaggagc acctctgcct ggctgagaac
     2341 aaaatggcca tccagagcag agacgtggca cagttcagaa atgttgtaac gcagttagaa
     2401 gcagatttgg acattaccaa aaggcaactc gggacagaac gttttgaaag ggaaagggct
     2461 gtccaagaac ttcgccgcca gaattactca agcaatgcct ataatttggg tccaatgaag
     2521 ccaaatacaa aatgccactc accagagcgt gctcaccatc gatctcctga ccgaggcctc
     2581 gaccgatcat tggaagagta agtgtgcaca ggacctggag tgtagccatg ttcacagtga
     2641 gatttgagga agcgaggaaa ctgatactaa acctggttaa taagacatga tgacaccaaa
     2701 aataaagggt tttcatctat aaa
//
