LOCUS       NM_021884               1952 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus tumor susceptibility gene 101 (Tsg101), transcript
            variant 1, mRNA.
ACCESSION   NM_021884
VERSION     NM_021884.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Munir,J., Sadri,M. and Zempleni,J.
  TITLE     Tsg101 knockout in the mammary gland leads to a decrease in small
            extracellular vesicles in milk from C57BL/6J dams and contributes
            to leakiness of the gut mucosa and reduced postnatal weight gain in
            suckling pups
  JOURNAL   J Nutr Biochem 135, 109782 (2025)
   PUBMED   39424203
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   He,Y., Wang,H., Hong,T., Hu,L., Gao,C., Gao,L., Cui,Y., Tan,R.,
            Pu,D. and Wu,J.
  TITLE     HFM1 is essential for the germ cell intercellular bridge transport
            in primordial follicle formation in mice
  JOURNAL   Cell Mol Life Sci 82 (1), 28 (2024)
   PUBMED   39725823
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1952)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1952)
  AUTHORS   Jeong,J., Kang,I., Kim,Y., Ku,K.B., Park,J.H., Kim,H.J., Kim,C.W.,
            La,J., Jung,H.E., Kim,H.C., Choi,Y.J., Kim,J., Kim,J. and Lee,H.K.
  TITLE     Regulation of c-SMAC formation and AKT-mTOR signaling by the
            TSG101-IFT20 axis in CD4+ T cells
  JOURNAL   Cell Mol Immunol 20 (5), 525-539 (2023)
   PUBMED   37029318
  REMARK    GeneRIF: Regulation of c-SMAC formation and AKT-mTOR signaling by
            the TSG101-IFT20 axis in CD4[+] T cells.
REFERENCE   5  (bases 1 to 1952)
  AUTHORS   Jiang,Y., Ou,Y. and Cheng,X.
  TITLE     Role of TSG101 in cancer
  JOURNAL   Front Biosci (Landmark Ed) 18 (1), 279-288 (2013)
   PUBMED   23276921
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1952)
  AUTHORS   Rountree,M.R., Bachman,K.E. and Baylin,S.B.
  TITLE     DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex
            at replication foci
  JOURNAL   Nat Genet 25 (3), 269-277 (2000)
   PUBMED   10888872
REFERENCE   7  (bases 1 to 1952)
  AUTHORS   Wagner,K.U., Dierisseau,P. and Hennighausen,L.
  TITLE     Assignment of the murine tumor susceptibility gene 101 (tsg101) and
            a processed tsg101 pseudogene (tsg101-ps1) to mouse chromosome 7
            band B5 and chromosome 15 band D1 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 84 (1-2), 87-88 (1999)
   PUBMED   10343110
REFERENCE   8  (bases 1 to 1952)
  AUTHORS   Wagner,K.U., Dierisseau,P., Rucker,E.B. 3rd, Robinson,G.W. and
            Hennighausen,L.
  TITLE     Genomic architecture and transcriptional activation of the mouse
            and human tumor susceptibility gene TSG101: common types of shorter
            transcripts are true alternative splice variants
  JOURNAL   Oncogene 17 (21), 2761-2770 (1998)
   PUBMED   9840940
REFERENCE   9  (bases 1 to 1952)
  AUTHORS   Li,L. and Cohen,S.N.
  TITLE     Tsg101: a novel tumor susceptibility gene isolated by controlled
            homozygous functional knockout of allelic loci in mammalian cells
  JOURNAL   Cell 85 (3), 319-329 (1996)
   PUBMED   8616888
REFERENCE   10 (bases 1 to 1952)
  AUTHORS   Maucuer,A., Camonis,J.H. and Sobel,A.
  TITLE     Stathmin interaction with a putative kinase and coiled-coil-forming
            protein domains
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3100-3104 (1995)
   PUBMED   7724523
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC090123.68.
            
            On Mar 4, 2023 this sequence version replaced NM_021884.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150369.1, SRR7345562.1358223.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC090123.68        57454-57601
            149-233             AC090123.68        63960-64044
            234-299             AC090123.68        66612-66677
            300-463             AC090123.68        68354-68517
            464-590             AC090123.68        70245-70371
            591-657             AC090123.68        73834-73900
            658-749             AC090123.68        76304-76395
            750-952             AC090123.68        84862-85064
            953-1192            AC090123.68        86261-86500
            1193-1952           AC090123.68        87718-88477
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 30.62 cM"
     gene            1..1952
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="tumor susceptibility gene 101"
                     /db_xref="GeneID:22088"
                     /db_xref="MGI:MGI:106581"
     exon            1..148
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1282
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="isoform 1 is encoded by transcript variant 1; tumor
                     susceptibility gene 101 protein; ESCRT-I complex subunit
                     TSG101"
                     /codon_start=1
                     /product="tumor susceptibility gene 101 protein isoform 1"
                     /protein_id="NP_068684.1"
                     /db_xref="CCDS:CCDS21291.1"
                     /db_xref="GeneID:22088"
                     /db_xref="MGI:MGI:106581"
                     /translation="MAVSESQLKKMMSKYKYRDLTVRQTVNVIAMYKDLKPVLDSYVF
                     NDGSSRELVNLTGTIPVRYRGNIYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKH
                     VDANGKIYLPYLHDWKHPRSELLELIQIMIVIFGEEPPVFSRPTVSASYPPYTATGPP
                     NTSYMPGMPSGISAYPSGYPPNPSGYPGCPYPPAGPYPATTSSQYPSQPPVTTVGPSR
                     DGTISEDTIRASLISAVSDKLRWRMKEEMDGAQAELNALKRTEEDLKKGHQKLEEMVT
                     RLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYA
                     EENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY"
     exon            149..233
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            234..299
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            300..463
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            464..590
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            591..657
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            658..749
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            750..952
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1192
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1952
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1505..1510
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="hexamer: AATAAA"
     polyA_site      1523
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="major polyA site"
     regulatory      1584..1589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="hexamer: AACAAG"
     polyA_site      1607
                     /gene="Tsg101"
                     /gene_synonym="CC2"
     regulatory      1918..1923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsg101"
                     /gene_synonym="CC2"
                     /note="hexamer: AACAAA"
     polyA_site      1952
                     /gene="Tsg101"
                     /gene_synonym="CC2"
ORIGIN      
        1 ggcttcctgc ttcgcagggg cggggcggcg gcggcggcga ttgtctggcg gcctggggcg
       61 gccccgtggc gcctaccctc tgcctgtggg gacggaggag cgcgccatgg cggtgtccga
      121 gagtcagctg aagaagatga tgtccaagta caaatataga gatctaaccg tccgtcaaac
      181 tgtcaatgtc atcgctatgt acaaagatct caaacctgta ttggattcat atgtttttaa
      241 tgatggcagt tccagggagc tggtgaacct cactggtaca atcccagtgc gttatcgagg
      301 taatatatat aatattccaa tatgcctgtg gctgctggac acatacccat ataacccccc
      361 tatctgtttt gttaagccta ctagttcaat gactattaaa acaggaaagc atgtggatgc
      421 aaatgggaaa atctacctac cttatctaca tgactggaaa catccacggt cagagttgct
      481 ggagcttatt caaatcatga ttgtgatatt tggagaggag cctccagtgt tctcccggcc
      541 tactgtttct gcatcctacc caccatacac agcaacaggg ccaccaaata cctcctacat
      601 gccaggcatg ccaagtggaa tctctgcata tccatctgga taccctccca accccagtgg
      661 ttatcctggc tgtccttacc cacctgctgg cccataccct gccacaacaa gctcacagta
      721 cccttcccag cctcctgtga ccactgttgg tcccagcaga gatggcacaa tcagtgagga
      781 cactatccgt gcatctctca tctcagcagt cagtgacaaa ctgagatggc ggatgaagga
      841 ggaaatggat ggtgcccagg cagagcttaa tgccttgaaa cgaacagagg aagatctgaa
      901 aaaaggccac cagaaactgg aagagatggt cacccgctta gatcaagaag tagctgaagt
      961 tgataaaaac atagaacttt tgaaaaagaa ggatgaagaa ctaagttctg ctctggagaa
     1021 aatggaaaat caatctgaaa ataatgatat tgatgaagtt atcattccca cagccccact
     1081 gtataaacag attctaaatc tgtatgcaga ggaaaatgct attgaagaca ctatctttta
     1141 ccttggagaa gctttgcggc ggggagtcat agacctggat gtgttcctga aacacgtccg
     1201 cctcctgtcc cgtaaacagt tccagctaag ggcactaatg caaaaggcaa ggaagactgc
     1261 gggccttagt gacctctact gacatgtgct gtcagctgga gaccgacctc tccgtaaagc
     1321 attcttttct tcttcttttt ctcatcagta gaacccacaa taagttattg cagtttatca
     1381 ttcaagtgtt aaatattttg aatcaataat atattttctg tttcctttgg gtaaaaactg
     1441 gcttttatta atgcactttc taccctctgt aagcgtctgt gctgtgctgg gactgactgg
     1501 gctaaataaa atttgttgca taatttttct ctttcttgga cttgagataa gatgcacaaa
     1561 agaagcctgg cttctcatta ataaacaagg cacagtgttc atcttaagtt gcagttcagt
     1621 attctgtagg ttaaagataa gttcccttta gtgtagaatg tcatgcagag aaatgtatat
     1681 attggaggat atgtttattt cattctagaa ccaactgaac catggttcaa aactttttta
     1741 atcaagaatt caattcccgc catttgcatt aggcagctca caactgtatt ctagctttgg
     1801 ggatccaata ttgtcttctg gtctctgtgg ccactacact catatgtaca tagacataga
     1861 tacatacttt aaaaaaaaaa aaaaacttgg gaaaatgtag taagactgtt tcaaataaac
     1921 aaacactgag catgctgggt gatttaaagg aa
//
