LOCUS       NM_199033               1787 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus tRNA splicing endonuclease subunit 2 (Tsen2),
            transcript variant 1, mRNA.
ACCESSION   NM_199033 XM_355804
VERSION     NM_199033.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Markiewicz-Potoczny,M., Lobanova,A., Loeb,A.M., Kirak,O.,
            Olbrich,T., Ruiz,S. and Lazzerini Denchi,E.
  TITLE     TRF2-mediated telomere protection is dispensable in pluripotent
            stem cells
  JOURNAL   Nature 589 (7840), 110-115 (2021)
   PUBMED   33239785
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Bopp,S.E., Ramachandran,V., Henson,K., Luzader,A., Lindstrom,M.,
            Spooner,M., Steffy,B.M., Suzuki,O., Janse,C., Waters,A.P., Zhou,Y.,
            Wiltshire,T. and Winzeler,E.A.
  TITLE     Genome wide analysis of inbred mouse lines identifies a locus
            containing Ppar-gamma as contributing to enhanced malaria survival
  JOURNAL   PLoS One 5 (5), e10903 (2010)
   PUBMED   20531941
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153828.8.
            
            On Sep 1, 2022 this sequence version replaced NM_199033.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061473.1, AK134697.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC153828.8         165415-165452
            39-247              AC153828.8         168546-168754
            248-329             AC153828.8         170637-170718
            330-366             AC153828.8         174715-174751
            367-874             AC153828.8         180317-180824
            875-952             AC153828.8         182104-182181
            953-1003            AC153828.8         186601-186651
            1004-1142           AC153828.8         189420-189558
            1143-1179           AC153828.8         195287-195323
            1180-1291           AC153828.8         196586-196697
            1292-1381           AC153828.8         197620-197709
            1382-1787           AC153828.8         198655-199060
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.58 cM"
     gene            1..1787
                     /gene="Tsen2"
                     /note="tRNA splicing endonuclease subunit 2"
                     /db_xref="GeneID:381802"
                     /db_xref="MGI:MGI:2141599"
     exon            1..38
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            39..247
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1441
                     /gene="Tsen2"
                     /EC_number="4.6.1.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tRNA-splicing endonuclease subunit Sen2; tRNA-intron
                     endonuclease Sen2; tRNA splicing endonuclease 2 homolog"
                     /codon_start=1
                     /product="tRNA-splicing endonuclease subunit Sen2 isoform
                     1"
                     /protein_id="NP_950198.1"
                     /db_xref="CCDS:CCDS20440.1"
                     /db_xref="GeneID:381802"
                     /db_xref="MGI:MGI:2141599"
                     /translation="MAEAVFRAPKRKRRVYESYESPLPIPFGQDQGPRKEFRIFQAEM
                     ISNNVVVRGTEDMEQLYGKGYFGKGILSRSRPNFTIANPTLAARWKGVQTDMPIITSE
                     KYQHRVEWARDFLRRQGHDESTVQKILTDYTEPLELPCREEKEETPQHEPLSSKADSS
                     LEGRVEKDELPVTPGGAGQSDDLPGLGTHSDCLQEGPGHATLAAASPSSHNGHVAEDP
                     EVLPQETLVPQGGLWPEASSQAAGEKRAAHEYVLIEEELCGAQEEEAAAASDEKLLKR
                     KKLVCRRNPYRIFEYLQLSLEEAFFLAYALGCLSIYYEKEPLTIVKLWQAFTAVQPTF
                     RTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRR
                     PFSWKSLAALSRVSGNVSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVS
                     SRERSDQDEL"
     exon            248..329
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            330..366
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            367..874
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            875..952
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1003
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1142
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1179
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1291
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1381
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1787
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1769..1774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsen2"
                     /note="hexamer: AATAAA"
     polyA_site      1787
                     /gene="Tsen2"
                     /note="major polyA site"
ORIGIN      
        1 ggttgccggg taacggtggg cgcgcgtggc ggcgaaagag acctgttatc cttgaagaat
       61 ggcagaggca gtcttccgtg ctcctaagag gaaaagaaga gtctatgaga gttacgagtc
      121 accactgccc atcccctttg gtcaggacca aggtcccagg aaggaattcc gaatattcca
      181 ggctgaaatg attagcaaca atgtggtggt gaggggcaca gaggacatgg agcagctgta
      241 tgggaagggt tactttggaa aggggattct ttccagaagc cgtccaaact tcacaatcgc
      301 caaccccacg ctggctgcca gatggaaagg tgtgcagaca gacatgccta taatcacctc
      361 agagaagtat cagcatcggg tggaatgggc cagggacttc ctgcgtaggc agggccacga
      421 tgagagcact gtgcagaaga tccttacaga ctacacagag ccactggagc ttccttgcag
      481 agaggagaaa gaagagaccc cacagcacga gccactgagc tccaaggccg actcctccct
      541 ggaaggcaga gtggaaaagg atgaactccc cgtgacccct ggaggtgctg gacagtcaga
      601 tgacctaccc ggtctcggca cacactcaga ctgcctgcag gaggggcctg gccatgccac
      661 actcgctgcg gctagtccat ctagccataa tgggcatgtg gcagaggacc ccgaggttct
      721 cccccaggaa accctcgttc cacagggtgg cctttggcct gaagccagca gccaggccgc
      781 aggagagaaa agggctgctc acgagtatgt gctcatagag gaagagctgt gtggtgccca
      841 ggaggaggag gcggcggcgg catcagacga gaagttgctg aaaaggaaga aacttgtgtg
      901 cagaagaaat ccatacagga tctttgagta tctgcagctc agcctagaag aggctttctt
      961 cctggcatac gccctgggat gcctaagtat ttactatgag aaggagcctc tgacaatagt
     1021 gaaactctgg caagccttca ctgcagtcca gcccaccttc aggacaacct acatggccta
     1081 ccactacttc cgaagtaaag gctgggtgcc caaagtgggg ctcaagtatg gaacagatct
     1141 gctgctgtat cggaaaggcc ctccatttta ccatgcaagc tactcggtca tcattgagct
     1201 cctggatgac aactatgagg gctccctccg ccggcctttc agctggaagt cgctggccgc
     1261 cctgagcaga gtctcgggga acgtctccaa ggagctgatg ctgtgttatt tgattaagcc
     1321 ctctaccatg actgctgaag acatggagac cccagaatgc atgaagagga ttcaagttca
     1381 ggaggtgatt ctcagccgat gggtttcttc ccgagagaga agtgaccaag atgagctttg
     1441 accttccaat cccagagtcc acagttcacc aacagctctg actgtgtgcc atcggcctgc
     1501 cccgaaggag caccggcaca tttggggatg cacaggaccc cgtggtctgt tgctgtaatg
     1561 gaaaaatcta ctctgtagct cagcttaccc aaggacttca accagttacc cacgtgactg
     1621 gaaacccatt gacggtttgg aaaccttgaa atactgagtg acgggtgcat tctcagtgaa
     1681 ctcactattt gacaggttgg tgcattgaac acaaaactat gtagtactgg ctgtgagaga
     1741 tgcatcttgc ttggagaact tcttataaaa taaaagtgag aagtgaa
//
