LOCUS       NM_001286720            6348 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 7,
            mRNA.
ACCESSION   NM_001286720
VERSION     NM_001286720.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Chen,H., Liu,C., Cui,S., Xia,Y., Zhang,K., Cheng,H., Peng,J.,
            Yu,X., Li,L., Yu,H., Zhang,J., Zheng,J.S. and Zhang,B.
  TITLE     Intermittent fasting triggers interorgan communication to suppress
            hair follicle regeneration
  JOURNAL   Cell 188 (1), 157-174 (2025)
   PUBMED   39674178
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Rossetti,C.L., Alves,B.L., Pecanha,F.L.M., Franco,A.T., Nose,V.,
            Carneiro,E.M., Lew,J., Bernal-Mizrachi,E. and
            Werneck-de-Castro,J.P.
  TITLE     Defining the In Vivo Role of mTORC1 in Thyrocytes by Studying the
            TSC2 Conditional Knockout Mouse Model
  JOURNAL   Thyroid 34 (8), 1047-1057 (2024)
   PUBMED   38661550
  REMARK    GeneRIF: Defining the In Vivo Role of mTORC1 in Thyrocytes by
            Studying the TSC2 Conditional Knockout Mouse Model.
REFERENCE   3  (bases 1 to 6348)
  AUTHORS   Marques,P., Burillo,J., Gonzalez-Blanco,C., Jimenez,B., Garcia,G.,
            Garcia-Aguilar,A., Iglesias-Fortes,S., Lockwood,A. and Guillen,C.
  TITLE     Regulation of TSC2 lysosome translocation and mitochondrial
            turnover by TSC2 acetylation status
  JOURNAL   Sci Rep 14 (1), 12521 (2024)
   PUBMED   38822085
  REMARK    GeneRIF: Regulation of TSC2 lysosome translocation and
            mitochondrial turnover by TSC2 acetylation status.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6348)
  AUTHORS   Ye,J., Xu,Y., Ren,Q., Liu,L. and Sun,Q.
  TITLE     Nutrient deprivation induces mouse embryonic diapause mediated by
            Gator1 and Tsc2
  JOURNAL   Development 151 (7) (2024)
   PUBMED   38603796
  REMARK    GeneRIF: Nutrient deprivation induces mouse embryonic diapause
            mediated by Gator1 and Tsc2.
REFERENCE   5  (bases 1 to 6348)
  AUTHORS   Alesi,N., Khabibullin,D., Rosenthal,D.M., Akl,E.W., Cory,P.M.,
            Alchoueiry,M., Salem,S., Daou,M., Gibbons,W.F., Chen,J.A.,
            Zhang,L., Filippakis,H., Graciotti,L., Miceli,C., Monfregola,J.,
            Vilardo,C., Morroni,M., Di Malta,C., Napolitano,G., Ballabio,A. and
            Henske,E.P.
  TITLE     TFEB drives mTORC1 hyperactivation and kidney disease in Tuberous
            Sclerosis Complex
  JOURNAL   Nat Commun 15 (1), 406 (2024)
   PUBMED   38195686
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6348)
  AUTHORS   Olsson,P.G., Lohning,C., Horsley,S., Kearney,L., Harris,P.C. and
            Frischauf,A.
  TITLE     The mouse homologue of the polycystic kidney disease gene (Pkd1) is
            a single-copy gene
  JOURNAL   Genomics 34 (2), 233-235 (1996)
   PUBMED   8661056
REFERENCE   7  (bases 1 to 6348)
  AUTHORS   Xu,L., Sterner,C., Maheshwar,M.M., Wilson,P.J., Nellist,M.,
            Short,P.M., Haines,J.L., Sampson,J.R. and Ramesh,V.
  TITLE     Alternative splicing of the tuberous sclerosis 2 (TSC2) gene in
            human and mouse tissues
  JOURNAL   Genomics 27 (3), 475-480 (1995)
   PUBMED   7558029
REFERENCE   8  (bases 1 to 6348)
  AUTHORS   Pilz,A., Glenister,P., Povey,S., Lyon,M. and Abbott,C.
  TITLE     The mouse homologue of the human tuberous sclerosis 2 (TSC2) gene
            maps to chromosome 17, but does not fall within the tw18 or th20
            deletions
  JOURNAL   Genomics 26 (2), 420-421 (1995)
   PUBMED   7601476
REFERENCE   9  (bases 1 to 6348)
  AUTHORS   Olsson,P.G., Sutherland,H.F., Nowicka,U., Korn,B., Poustka,A. and
            Frischauf,A.M.
  TITLE     The mouse homologue of the tuberin gene (TSC2) maps to a conserved
            synteny group between mouse chromosome 17 and human 16p13.3
  JOURNAL   Genomics 25 (1), 339-340 (1995)
   PUBMED   7774953
REFERENCE   10 (bases 1 to 6348)
  AUTHORS   Kim,K.K., Pajak,L., Wang,H. and Field,L.J.
  TITLE     Cloning, developmental expression, and evidence for alternative
            splicing of the murine tuberous sclerosis (TSC2) gene product
  JOURNAL   Cell Mol Biol Res 41 (6), 515-526 (1995)
   PUBMED   8777431
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132367.3.
            
            On Mar 30, 2022 this sequence version replaced NM_001286720.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                AC132367.3         11865-11947
            84-250              AC132367.3         12374-12540
            251-337             AC132367.3         13486-13572
            338-448             AC132367.3         14506-14616
            449-593             AC132367.3         15399-15543
            594-711             AC132367.3         16377-16494
            712-760             AC132367.3         17742-17790
            761-886             AC132367.3         18114-18239
            887-960             AC132367.3         19144-19217
            961-1087            AC132367.3         20883-21009
            1088-1231           AC132367.3         21817-21960
            1232-1369           AC132367.3         22721-22858
            1370-1473           AC132367.3         23322-23425
            1474-1555           AC132367.3         23654-23735
            1556-1711           AC132367.3         24915-25070
            1712-1828           AC132367.3         25503-25619
            1829-1951           AC132367.3         28886-29008
            1952-2058           AC132367.3         29695-29801
            2059-2209           AC132367.3         29983-30133
            2210-2332           AC132367.3         31172-31294
            2333-2467           AC132367.3         33914-34048
            2468-2657           AC132367.3         35352-35541
            2658-2751           AC132367.3         36297-36390
            2752-2854           AC132367.3         36684-36786
            2855-2949           AC132367.3         36954-37048
            2950-3078           AC132367.3         38133-38261
            3079-3243           AC132367.3         39493-39657
            3244-3396           AC132367.3         39827-39979
            3397-3509           AC132367.3         40067-40179
            3510-3722           AC132367.3         41187-41399
            3723-3926           AC132367.3         42188-42391
            3927-3995           AC132367.3         43028-43096
            3996-4126           AC132367.3         44020-44150
            4127-4617           AC132367.3         44438-44928
            4618-4693           AC132367.3         45130-45205
            4694-4780           AC132367.3         45374-45460
            4781-4967           AC132367.3         46115-46301
            4968-5107           AC132367.3         46454-46593
            5108-5186           AC132367.3         47202-47280
            5187-5395           AC132367.3         47422-47630
            5396-6348           AC132367.3         47719-48671
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.41 cM"
     gene            1..6348
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="TSC complex subunit 2"
                     /db_xref="GeneID:22084"
                     /db_xref="MGI:MGI:102548"
     exon            1..83
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            84..250
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..5557
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="isoform 5 is encoded by transcript variant 7;
                     tuberin; tuberous sclerosis 2 protein homolog"
                     /codon_start=1
                     /product="tuberin isoform 5"
                     /protein_id="NP_001273649.1"
                     /db_xref="GeneID:22084"
                     /db_xref="MGI:MGI:102548"
                     /translation="MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEI
                     LRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVL
                     TLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEEL
                     AEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVS
                     LQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNM
                     CRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVV
                     SYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVE
                     ELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLME
                     RFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLA
                     TQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLG
                     LLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRAD
                     SLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLG
                     YLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSM
                     LSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIY
                     RCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHL
                     YRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITK
                     GLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAA
                     EAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMM
                     ARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDL
                     QGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSA
                     PYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTG
                     NTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAK
                     PPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDF
                     EAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSF
                     QPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATG
                     EESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAE
                     KVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYV
                     GEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTY
                     CWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQF
                     NFVHVIITPLDYKCNLLTLQCRKDGPACKCEWWRQPGEIVVWALPVVMELTVTILLCH
                     LQMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKA
                     PAQAPEATPTYETGQRKRLISSVDDFTEFV"
     exon            251..337
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            338..448
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            449..593
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            594..711
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            712..760
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            761..886
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            887..960
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1087
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1231
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1369
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1473
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1555
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1711
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1828
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1951
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2058
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2209
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2332
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2467
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2657
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2751
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2752..2854
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..2949
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3078
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3079..3243
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3244..3396
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3397..3509
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3722
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..3926
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..3995
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            3996..4126
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            4127..4617
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            4618..4693
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            4694..4780
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            4781..4967
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            4968..5107
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            5108..5186
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            5187..5395
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     exon            5396..6348
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5626..5631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="hexamer: AATAAA"
     polyA_site      5674
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="major polyA site"
     regulatory      6087..6092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="hexamer: AATATA"
     polyA_site      6107
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
     regulatory      6321..6326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
                     /note="hexamer: AATAAA"
     polyA_site      6348
                     /gene="Tsc2"
                     /gene_synonym="Nafld; Tcs2"
ORIGIN      
        1 gcgcttccgg cagctgccct gggccggggg tgtgcgccct tccctgggtg caggaggacc
       61 tgcgcgcgaa aggcgcgcgc gggagcagtt ctctagtact gccctgccca ccatggccaa
      121 accaacaagc aaagattccg gcttgaagga gaagttcaag atactgttgg gattgggaac
      181 atcgaggcca aatcccaggt gtgcagaagg caaacagact gagtttatca tcacatcgga
      241 aatcttgaga gaactgagtg gtgaatgcgg cctcaacaat cgcatccgaa tgatagggca
      301 gatctgtgat gtggcaaaaa ctaagaagct tgaggagcat gcagtggagg cactttggaa
      361 ggctgtctca gacttgctac agccagagcg gccaccagag gcccggcatg cagttctcac
      421 cttattgaag gccattgtac agggacaggg tgatcgtttg ggagttctca gagccctctt
      481 cttcaaagtg atcaaggact acccctccaa tgaagacctc catgaaaggc tagaagtttt
      541 taaggccctc acagacaatg ggaggcacat tacctatttg gaagaagaac tggcagagtt
      601 tgtcctgcag tggatggatg ttggcttgtc ctcagaattc cttctggtac ttgtcaacct
      661 ggtcaagttc aacagctgtt accttgacga atacattgca tcaatggttc acatgatttg
      721 tctgctatgc atccggacag tgtcctctgt ggacattgag gtgtccttgc aagtgctgga
      781 tgctgtggtc tgctacaact gcttaccagc cgagagcctg cctctgttca ttatcaccct
      841 gtgccgcacc atcaatgtca aggagctgtg tgagccttgc tggaagttga tgcgtaacct
      901 tctgggcacc cacctgggcc acagcgccat ctacaacatg tgccgcatca tggaggacag
      961 atcctacatg gaagatgccc cactgctgag aggagctgtg ttctttgtag ggatggcact
     1021 ctggggagct caccggctct actctctcaa gaactccccc acatctgtgc tgccgtcttt
     1081 ttatgaggct atgacctgtc ccaatgaggt ggtgtcatat gagattgttc tgtccataac
     1141 aagactcatc aagaagtata ggaaggagct ccaggctgtg acatgggata ttctgctgga
     1201 catcattgaa cgactacttc agcaactcca gaacctggac agcccggaac tcaagaccat
     1261 cgtccatgac ctgctgacca ctgtggagga gctatgtgac cagaacgagt tccatggctc
     1321 gcaggaaaga tactatgaac tggtggagag ctatgcagac cagagacctg aatcctctct
     1381 cttaaacttg atatcctaca gagcccagtc catccaccct gccaaggatg gctggatcca
     1441 gaacttgcag ttgttgatgg agaggttctt caggaatgag tgccgcagcg ccgtgcgcat
     1501 caaggtgttg gatgttctgt ccttcgtgct gctgatcaac aggcagttct atgaggagga
     1561 gctgattaac tcggtggtca tctcgcagct ctcccacatt cccgaggata aggaccatca
     1621 ggtccgaaag ctggctactc agctgctggt ggacctggca gaggggtgcc acacccacca
     1681 cttcaacagt ctgctggaca tcattgaaaa ggtgatggcg cgctcactct ctccaccccc
     1741 ggagctggaa gaaagggacc tggccatgca ctcggcctcc ctggaggacg tgaagaccgc
     1801 ggtcctgggg ctcctggtca tccttcagac caagctctac accttgcctg ccagccacgc
     1861 cactcgagtg tatgagagcc tcattagtca catccagctc cattacaagc acggctactc
     1921 cctgcccatt gctagcagca tccgactaca ggcctttgac ttcctgctgc tactgcgggc
     1981 tgactcgctg catcgactgg gcctgcccaa caaggatggg gtcgtgagat tcagccctta
     2041 ctgcctctgt gactgcatgg aactggatag agcctcagag aagaaagcca gtgggcccct
     2101 ttcacctcca actgggcccc ctagccctgt gcctatgggc cctgctgtgc ggctcggcta
     2161 cctaccctac tccctgctct tccgtgtcct gttgcagtgt ttgaagcagg agagcgactg
     2221 gaaggtgctg aagctggtgc tcagcaggct gccggagtca ctgcgctaca aagtcctcat
     2281 cttcacctcc ccctgcagtg tcgaccagct gtcttctgcc ctctgctcca tgctttcagc
     2341 tccaaagacc cttgagcggc tccgaggtac cccagaaggc ttctcgagaa ctgacctgca
     2401 cttggctgtg gttcccgtgc taacagcatt aatatcttat cacaactatt tggacaagac
     2461 cagacagcgt gagatggtgt actgcctgga acaaggcctt atctaccgct gcgccagcca
     2521 gtgcgtggta gccttggcca tctgcagtgt ggagatgccc gacatcatca tcaaggcgct
     2581 tcctgtcctg gtggtgaagc tcacacacat ttctgccaca gccagcatgg ccatcccgct
     2641 cctggagttc ctgtctactt tggccaggct gccccacctc tacaggaact ttgctgcaga
     2701 gcagtatgcc agcgtgtttg ccatctcctt gccatacacc aacccctcca agttcaacca
     2761 gtacattgtg tgtctggctc accacgtcat agccatgtgg ttcatcaggt gccgactgcc
     2821 ctttcggaag gatttcgtcc cttatatcac taagggtttg cgttccaatg tcctcctgtc
     2881 ttttgatgat acccctgaga aggacagttt cagagcacgg agcaccagtc ttaatgagag
     2941 acccaaaagt ttgaggatag ccagagcccc caaacaaggc ctgaataact ctccacctgt
     3001 gaaagaattc aaagagagct gtgcagccga ggccttccgg tgccgcagca tcagtgtatc
     3061 tgaacatgtg gtccgcagca gaatacagac atcccttacg agcgccagcc tggggtctgc
     3121 ggatgagaac tctatggccc aggctgatga caacttgaag aatctccact tggagctcac
     3181 agaaacatgt ctggacatga tggcccgata tgtgttctcc aatttcactg cagtccccaa
     3241 gaggtcccct gtgggagagt tcctcctggc agggggtagg accaaaacct ggctggttgg
     3301 aaacaagctt gtcactgtga caacaagtgt gggaactggt acacggtcgc tgctgggcct
     3361 ggactctggg gacctgcagg gtggctcgga ttcaagctct gatcctagca cacatgtgag
     3421 gcagacaaag gaagcaccgg ccaaactgga gtcccaggct gggcagcagg tgtcccgtgg
     3481 ggcccgggac cgggtccgct ccatgtcagg gggccatggc cttcgagttg gtgtcctgga
     3541 tacttcagct ccctattccc caggtggctc tgcttctctg ggaccacaga cggcggtggc
     3601 agcgaagccc gagaagcccc ctgcaggagc ccagcttcca acagcagaga agacgaatct
     3661 ggcagcctat gtgcctttgt taacccaggg ctgggcagaa atcttagtcc gcagacccac
     3721 aggaaacacc agctggctga tgagcttgga gaacccgctc agccccttct cctcagacat
     3781 caacaacatg cccctgcaag agctgtccaa tgcccttatg gctgctgaac gtttcaagga
     3841 gcaccgggac acggccctgt acaagtcatt gtcagtgcca gcggctggca ccgccaagcc
     3901 tcctactctc ccacgctcta atacagtggc ttctttctcc tccctgtacc agcccagctg
     3961 ccaaggacag ctgcacagga gcgtttcctg ggcagactca gccatggtcc ttgaggaggg
     4021 aagtccagga gagactcagg tgccagtgga gccccccgaa ttggaggatt tcgaggcggc
     4081 gttaggtaca gacaggcact gccagcggcc tgacacctac agccggtcat cctcagcatc
     4141 tagccaggaa gaaaagtccc atttggagga gcttgctgca gggggtatcc ccattgagcg
     4201 ggccatctcc tctgaggggg ctcggcctgc cgtggacctc tccttccaac cctcacagcc
     4261 tttgagcaag tctagctctt ctccggagtt gcagacctta caggacatcc ttggagacct
     4321 aggggacaag attgatattg gacggctgag tcctgaggct aaggtccggt cccagtcagg
     4381 gatcctggat ggggaagcag ctacctggtc agctacgggt gaagagagcc gtatcacagt
     4441 cccacctgaa ggtcctctgc cttccagttc tccccgctcc cccagtggcc tccggccccg
     4501 aggctatacc atctctgatt ccgctccatc acgaagggga aagagggtag aaagggataa
     4561 cttcaagagc agagctgcgg cctccagtgc tgagaaggtg ccaggcatca accctagctt
     4621 tgtgttccta cagctctacc attcgccctt ttttggtgat gagtccaata agcccatcct
     4681 attgcccaat gagtcctttg aacggtcagt acagctgctt gaccagattc catcctatga
     4741 cactcacaag attgctgtcc tgtatgtggg agaaggccag agcagcagtg agctggccat
     4801 cctgtcaaat gagcatggct cttacaggta cacggagttt ctgacaggcc tgggtcggct
     4861 tattgagctc aaggactgcc aaccagacaa ggtgtactta ggtggactgg atgtatgtgg
     4921 cgaggatgga cagttcacct actgctggca tgatgacatc atgcaagctg ttttccacat
     4981 tgccaccctg atgcccacca aggatgtgga caagcaccgc tgtgacaaga aacggcacct
     5041 gggcaatgac tttgtttcta tcatctacaa tgattctggt gaggacttca aactgggcac
     5101 cattaagggc cagttcaact ttgtccatgt gatcatcaca ccgctggact acaagtgcaa
     5161 cctattgacc ctgcagtgca ggaaagatgg ccctgcatgc aaatgtgagt ggtggcggca
     5221 gcctggggag atagtcgtgt gggcgctccc tgttgtgatg gagctcactg tcaccatcct
     5281 gctctgtcac ctccagatgg cctcacaggt acaccacagc cgatccaacc ccactgacat
     5341 ctatccctcc aagtggatcg caagactccg ccacattaag cgtctccgcc agaggatccg
     5401 tgaagaggtg cactattcca acccaagctt gcctctgatg caccctccag cccacaccaa
     5461 agccccagct caagcccctg aggctacacc cacctatgaa acaggccagc ggaagcgcct
     5521 catttcctcc gtggatgact tcacagagtt tgtgtgaggc cagcagatcc atgactagag
     5581 tcctgtggcc agccatgtag ctatcctgac tgtactccta catgaaataa actgcacacg
     5641 cacatctggc ccgtggagtt cagagagcag cacagacagc agtcagacag cttctttatt
     5701 tgactttgtc ttgtctgttt ggggttgggg cagaatccag gctagagctg cagatgagcc
     5761 cacagaagca ggcaggtgcc atcacataca ttctctttcc cccacccgcc atgggggcaa
     5821 gccccattgg tgggcttgtc cagtaacttc ttggcatctt tcatcccaca gtagtcctgc
     5881 ccttgctcag atctgaaacc agcagatctg cgtgctgcct ggccccagac aagggaggaa
     5941 aacaaggcca cggtggcccc aattgctgtc cagcacctgc ccgaggccag actcggatcc
     6001 agtaatagct gcagcttccc ctgtacccct gccctgggta acaatggacc cattttcagc
     6061 ccaagtagaa aatactgaca tacaaaaata tatacatttt aaccccatat aaattattga
     6121 tggtggacat agaaagcaga tggtagctag agggcaagta tgctgcagaa acaagagagg
     6181 taataactta ggggacggat aaaggagaag tgtagcttag ccctccctcc agcttagatt
     6241 tttagcaaga ggagatggcc acctaggggt agagctgggc gcagcccgct gaacctgagg
     6301 acttgggggg agggggggcg aataaattag catcttggag gctgggta
//
