LOCUS       NM_145520               4003 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TruB pseudouridine (psi) synthase family member 2
            (Trub2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_145520
VERSION     NM_145520.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4003)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   4  (bases 1 to 4003)
  AUTHORS   McIntyre,R.E., Nicod,J., Robles-Espinoza,C.D., Maciejowski,J.,
            Cai,N., Hill,J., Verstraten,R., Iyer,V., Rust,A.G., Balmus,G.,
            Mott,R., Flint,J. and Adams,D.J.
  TITLE     A Genome-Wide Association Study for Regulators of Micronucleus
            Formation in Mice
  JOURNAL   G3 (Bethesda) 6 (8), 2343-2354 (2016)
   PUBMED   27233670
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4003)
  AUTHORS   Dequeant,M.L., Glynn,E., Gaudenz,K., Wahl,M., Chen,J., Mushegian,A.
            and Pourquie,O.
  TITLE     A complex oscillating network of signaling genes underlies the
            mouse segmentation clock
  JOURNAL   Science 314 (5805), 1595-1598 (2006)
   PUBMED   17095659
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK090004.1, AK167294.1, AL845323.18 and AK081579.1.
            
            On Mar 13, 2014 this sequence version replaced NM_145520.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015285.1, AK167294.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-782               AK090004.1         833-1614
            783-2576            AK167294.1         759-2552
            2577-3643           AL845323.18        188166-189232       c
            3644-4003           AK081579.1         3574-3933
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 20.57 cM"
     gene            1..4003
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="TruB pseudouridine (psi) synthase family member 2"
                     /db_xref="GeneID:227682"
                     /db_xref="MGI:MGI:2442186"
     exon            1..169
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1056
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /EC_number="5.4.99.25"
                     /note="isoform a is encoded by transcript variant 1;
                     probable tRNA pseudouridine synthase 2; TruB pseudouridine
                     (psi) synthase homolog 2; mitochondrial mRNA pseudouridine
                     synthase Trub2; psi55 synthase TRUB2; tRNA pseudouridine
                     55 synthase TRUB2; truB pseudouridine synthase homolog 2;
                     pseudouridylate synthase TRUB2, mitochondrial"
                     /codon_start=1
                     /product="pseudouridylate synthase TRUB2, mitochondrial
                     isoform a"
                     /protein_id="NP_663495.3"
                     /db_xref="CCDS:CCDS15856.1"
                     /db_xref="GeneID:227682"
                     /db_xref="MGI:MGI:2442186"
                     /translation="MGSSGLARLQGLFAVYKPPGLKWLHLRETVELQLLKGLNAQQPP
                     APDQRVRFLLGPVEGSEEKKLTLRATNVPSLTTHRLVRGPAFTNLKIGVGHRLDVQAS
                     GVLVLAVGHGRSLLTDMYDAHLTKDYTVRGLLGKATDNFCEDGRLIEKTTYDHVTRER
                     LDRILAVIQGSHQKALVMYSNLDLKSQEAYEMAVQGVIRPMNKSPMLISGIRCLHFAP
                     PEFLLEVQCMHETQQQLRKLVHEIGLELKTSAVCTQVRRTRDGFFGLDDALLRTQWDL
                     HNIQDAIQAAAPRVAAELQKNLSLKLGHHQLPSTGQPWGLKDPSSTLELESCSGQ"
     exon            170..301
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            302..376
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            377..438
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            439..520
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            521..593
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            594..730
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            731..4003
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2550..2555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="hexamer: TATAAA"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="hexamer: AATACA"
     polyA_site      2577
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="major polyA site"
     polyA_site      2581
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
     regulatory      2832..2837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="hexamer: GATAAA"
     polyA_site      2860
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
     regulatory      3795..3800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3813
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
     regulatory      3836..3841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      3853
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
     polyA_site      4003
                     /gene="Trub2"
                     /gene_synonym="G430055L02Rik"
ORIGIN      
        1 acgcagcact tccgcccgca tccccgcttc tggatgatgt tgaaccgctg agttccagcc
       61 atgggttctt ctggcctagc tcggctgcag gggctcttcg ctgtctataa acctccgggg
      121 ctgaagtggc tgcacttgcg tgagaccgtg gagttgcaac tgcttaaagg tctcaatgcc
      181 cagcagcctc ctgctcctga tcagcgtgtt cgcttcttgc taggccctgt ggaaggcagt
      241 gaagagaaga agttgactct cagagccacc aatgtaccct ccctcaccac ccacagactt
      301 gtacgtggtc cagcattcac aaacctgaag atcggtgtgg gacatcgctt ggatgtccag
      361 gcttctggtg tgcttgtgct cgccgtggga catggacgca gcctcctcac tgacatgtat
      421 gatgctcatc tcaccaagga ttacacagtg cgtggcctcc tgggcaaagc tacagacaac
      481 ttctgtgaag atggacggct gatagagaag acaacgtatg accacgtgac cagagagcgg
      541 ctggaccgga tcctggctgt gatccaaggc tcccaccaaa aagctctggt gatgtactcc
      601 aacctggacc tgaaatccca ggaggcctat gagatggctg tgcaaggtgt gatcaggccc
      661 atgaacaagt cccccatgct catctctggc atccgctgcc tgcactttgc acctcccgag
      721 tttcttttgg aggtgcagtg tatgcacgag acgcagcagc agctgaggaa gctggtgcat
      781 gaaatcggcc tggagctgaa gaccagcgct gtctgcacgc aagtgcggcg gactcgggat
      841 ggcttcttcg gcctggatga tgccctcctg agaacccagt gggacctaca caacatccag
      901 gatgctatcc aggcggcagc tccccgggtg gcagcggaac tgcaaaagaa cttgagccta
      961 aagctgggcc accatcagct ccccagcact gggcaacctt ggggtctcaa ggacccaagc
     1021 tccactttgg agttggaaag ctgttcaggg cagtaaaggg ccaggtagca accagatgga
     1081 gagcaaacag gctcttcaca ggtcagaact ggtcactccg caggagtaat gagatcaggg
     1141 gcccctgagc cattatcctt gagacacaga ctaactgcag aggcaagcag gtgtggccat
     1201 gggggcctat cgtcccagct aattgggagg cccaggttag aatcttaaca gttcaaggcc
     1261 agcctagggt ttcttagtaa aaccttgact cagagtcagg catgatgtct caggcctgta
     1321 atcttagccc tccagaggta gaaaggaaaa tcgtgagttc aaggccagcc tgggctactt
     1381 gcagtgttca gcttttcaac tgatgggaaa tgccgctgcc tcagcactcc ttggccgtga
     1441 gcgatgatct tttcacagag ggaaagtctt ttttgctgtt tcaaggtgtc tcacagctga
     1501 gatctcagta ctactcagct gagccaggag gatggctctg agtttaaggc cagtctgaac
     1561 aagtctcctg gagtactttg tggtatgtgt cagtatttaa tcctgccagt caatcctgca
     1621 aacctgtaat tagcacctgg gtggtggaga ctggaagatc tggagttcaa ggtcatcttt
     1681 ggctacataa gttccaggct acatgaaacg ttatctctaa gacaatataa aaagcccaga
     1741 attatttttt aaaaggtttt ctttcttatg catgtgagta tttacatgca tgtatgtcca
     1801 cacctggtgc atcatggagg ctaggaaagg atgtaagagc cctgtggctg ggcttaaagg
     1861 cagttgtgag ccaccaagaa gatgctgaca atggaccact agtcctctgg aagacagctt
     1921 aaccactggt ccatttcttc agaatttaat ttaattgcct gccctgtgat acaatgctgt
     1981 aactatcccg tgccccggct gtaaacacag gctgatgatt gttggtgtct gcagtgtgaa
     2041 cactaacagg ctgatgattg ttggtgtctg cagtgtaaac actaacaggc tgatgattgt
     2101 tggtgtctgc agtgtgaacg aacactaaca ggctgatgat tgttggtgtc tgcagtgtga
     2161 acacttacag gctgatgatt gttggtgtct gcagtgtgaa cacttacagg ctgatgattg
     2221 ttggtgtctg cagtgtgaac acttacaggc tgatgattgt tggtgtctgc agtgtgaaca
     2281 ctaacaggct gatgattgtt ggtgtctgca gtgtgaacac taacaggctg atgattgttg
     2341 gtgtctgcag tgtgaacact tacaggctga tgattgttgg tgtctgcagt gtgaacacta
     2401 acaggctgat gattgttggt gtctgcagtg tgaacactaa caggctgatg attgttggtg
     2461 tctgcagtgt gaacactaac aggacagcaa gggatggtgt tcactcagtg gtgccggtgg
     2521 aaggcgtggt gcagagaaga catcgggttt ataaaataca gtggttttcc tctttgagaa
     2581 aggcatgtga tgttttaatt accctccaac tctcccttga ccccattgcc atcatgtccc
     2641 cctcttagcc tcaagtccgc tatctttgta agtccctgag accagttagt gtagggcaag
     2701 tgcatgggta tggggccatt tacagcctac cccctccgcc agcctcaagg tggtggggtc
     2761 catgagcccc tttgtgtggt aaataatatt aattgggggt ttccagcatg ccttagatta
     2821 cttagttccc agataaaggg cactgccttt gcacatgcaa gaagcctctg ggtttatttc
     2881 atctacttcc ttgccaagaa cccagagacg ccacctgcca tgttctgcct gggccagcca
     2941 ctcttactcc cctggacacc ctttggccag tttttactac cccttacccc atggtgtctt
     3001 cctctcttct ctgatctctc catgagtcta tccctcagac cccaagccct acctcctata
     3061 gactgtaggc atttttatta accagtcaga gataacttgg ggacaaggtt ccatagactt
     3121 ggtgcacatt gaagatcttc tcttcctgag gcaaccagac cttgggggcc agtatttagc
     3181 attacgacac ataacagacc aaacctcaac accccttcca tccctgccgg gaggttgact
     3241 ggctggttgt gtacagataa ccagaggtgc tttgagacta tcatgcccag aagtcagttt
     3301 tccagtactg tcccctgtcc ctggcttctg agacttgctc ctctccctgt gtaacctgcg
     3361 cagcaccttc cagtgttagg agaggtttcc aggtctgttc ttcatatgct gtcaccaagt
     3421 gtggtgtcct cagcagcatg gtctgtcatg ttctgctggg taaccaagag caatagcatt
     3481 agcttggatt gtttgaagga tctgggtgac ctgacaaagg gacgtatccc acagtggaca
     3541 ggagcatttt aaaaacctat gacttttggg acagagcttt agtccctgtg tagtgtacct
     3601 ccattcaagc ctctatatat atatatatat atatatatat atacacacac acacacacac
     3661 acacacatac atacatgtga atataaatgt ttaaatattt gttttcagga cagttttgct
     3721 gtgtaatctt ggaactcact ttgtagatta tgctgacctt gagctctgag cacctttgcc
     3781 tcctgagtgt taggattaaa gtgtgtgcca ctatatcagc ttaattatat tttttagtaa
     3841 acttagaaaa ctagtttcta tgtggctttt caaatatcct taatgttagt gacccctctc
     3901 cctgccacct aggggttttg taaagctgtt tttctccctg attttatttc tctctgtagt
     3961 aggtactttg cccccatgtg tagatagata ccttttcact ttc
//
