LOCUS       NM_028115               3795 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TruB pseudouridine (psi) synthase family member 1
            (Trub1), transcript variant 1, mRNA.
ACCESSION   NM_028115
VERSION     NM_028115.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Klein,A.D., Ferreira,N.S., Ben-Dor,S., Duan,J., Hardy,J., Cox,T.M.,
            Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Identification of Modifier Genes in a Mouse Model of Gaucher
            Disease
  JOURNAL   Cell Rep 16 (10), 2546-2553 (2016)
   PUBMED   27568557
REFERENCE   3  (bases 1 to 3795)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 3795)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY094716.1, AK031674.1 and AC140413.3.
            
            On Nov 18, 2009 this sequence version replaced NM_028115.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039287.1, AK031674.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY094716.1         2-23
            23-1718             AK031674.1         1-1696
            1719-3795           AC140413.3         151322-153398
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 52.25 cM"
     gene            1..3795
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /note="TruB pseudouridine (psi) synthase family member 1"
                     /db_xref="GeneID:72133"
                     /db_xref="MGI:MGI:1919383"
     exon            1..291
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1058
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /EC_number="5.4.99.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable tRNA pseudouridine synthase 1; TruB pseudouridine
                     (psi) synthase homolog 1; psi55 synthase TRUB1; tRNA
                     pseudouridine 55 synthase TRUB1; truB pseudouridine
                     synthase homolog 1; pseudouridylate synthase TRUB1"
                     /codon_start=1
                     /product="pseudouridylate synthase TRUB1 isoform 1"
                     /protein_id="NP_082391.1"
                     /db_xref="CCDS:CCDS29926.1"
                     /db_xref="GeneID:72133"
                     /db_xref="MGI:MGI:1919383"
                     /translation="MAAAEAEVVSPLIVDTAPDTSGTAEASVAASVAEAARTESQAPA
                     SKAALAAKLMSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWNKRQKQTLKV
                     GHGGTLDSAAQGVLVVGIGRGTKMLTSMLSGSKRYITIGELGKATDTLDSTGKVTEEK
                     PYDKITREDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKKGKVVEARPARPVTV
                     HSISLLKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCASVLELTRTKQGPFTLAQH
                     ALPEDRWTIDDIEQSLERCTSLLPEELTFKKLKSDNSSDQVISCGYITLRDTKREDDA
                     VKTL"
     exon            292..390
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            391..446
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            447..528
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            529..601
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            602..741
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            742..798
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            799..3795
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1675..1680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      1700
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /note="major polyA site"
     regulatory      3773..3778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3795
                     /gene="Trub1"
                     /gene_synonym="2610009I02Rik; 9030425C13Rik"
ORIGIN      
        1 ggtgtcatct ctgctcgcct cttggcccag gccgtgaaag catggcagct gcagaggcag
       61 aggtggtttc acctctgatt gtggacacag ctcctgacac ttcaggaaca gccgaggcct
      121 ctgtagcagc atcggtggcg gaagcggcca ggactgaatc gcaagccccg gcctccaagg
      181 ccgccctggc tgctaagctc atgtcgctga gcggcgtgtt cgccgtgcac aagcccaaag
      241 ggcccacttc agccgagctg ctgaaccggc tgaaggagaa gctgctggca gaagctggaa
      301 tgccttctcc agaatggaac aagaggcaaa agcagacatt gaaagtcgga cacggaggga
      361 cgctggacag tgcagcgcaa ggtgttctgg tggttgggat tggaagagga acaaaaatgt
      421 tgaccagtat gttgtcgggg tccaagaggt atatcaccat cggagaactg gggaaagcca
      481 cggacaccct ggattctact gggaaggtaa cggaagagaa gccgtatgat aaaataacac
      541 gagaagatat cgaaggcatt ctgcagaagt ttactgggaa tataatgcaa gtacccccgc
      601 tctattctgc gctgaagaag gatgggcaga gactctcaac gctaatgaag aaaggcaagg
      661 tggtggaggc cagaccggcc cggcccgtga ctgtccacag tatctccctc ctgaagttcc
      721 agcctccgtt cttcacgctg gatgttgaat gcggaggagg cttttacatc cggagcctgg
      781 tcagtgacat cggcaaagag ctctcttcct gtgccagcgt gctggagctg acccgcacca
      841 aacaggggcc cttcacactt gcacagcacg ccctccccga agacaggtgg accatcgatg
      901 acatcgaaca gtccctcgag cgctgcacgt ctcttctgcc ggaggagctg acatttaaaa
      961 aactaaaatc tgacaactcc agtgaccagg ttataagctg tggatatata actctcaggg
     1021 acacaaagag agaagatgac gcagttaaga cactgtgagg ccggcctggg aggatcattt
     1081 cctggttggt gtctgtcccg accaggaatg cccatacacc tcttgagttc tttgcaagat
     1141 gtcttaatgt ctctgaatga tggatctgat tggattcaga taaataagtt ttctgaattt
     1201 gatcttccct tagtcttttt cctatgaaaa agttaaacaa tattctaatg acagaatcct
     1261 gaagtctgag ctgcaggttc cactggtctc acagaagtgt caaacttgct ttgcttagac
     1321 tcggggaggg ggcgttcgtt gttttgaaat tctctgaata catttgaagt gttcagtgtc
     1381 gagctgtcct gtgtgtttgc agagcctatg gctgctggaa ggtgctggtg ggctgtactc
     1441 agctgtgact gtgtttctta aatcccggga atcaatacgt tgtcctaata catatctgta
     1501 aatgcacaaa ctcctgcttt ggccactgac agaatgtaaa tagcgtgaga cgtgattcat
     1561 ttcagatcct gtattcagca gagctcagct cctgtagccg tggactgtgg cagctatttc
     1621 tgtggtcaac cacatggtat caaagtacca aaaatgtgat tttctagaat ataaactaaa
     1681 gaatgtatct tgaattaaaa tgctctcctg cctgttcgtt agcgcctctc tgtgactgcg
     1741 gaaccctggt ccagtgacat ggtttctgta gttatgaaga atatgatcac attttcaaac
     1801 agcagatttt atgagtgctt cattgttttg ttatagtaat atctattctt ttattgtgtg
     1861 ttttgagttc ttgaagacaa ggaaactgag gacactggag aggcgaagac acactgcctg
     1921 tccagcaggc acggagtgta gattctgcac ccacccacag caggcggctc tcgctacttc
     1981 agctccaagg gacccagcac ccacttgtgg cctgtgggcc cctgtgaacg aacacccatg
     2041 cctccacaca catacataaa ataattactt taagatatcg agatttaacg caggatggcc
     2101 ccactcatta tgtagccaag actcctcctg tatctacctc ccaagtgtag aggttagagg
     2161 cataggccac tgactcctgc aggaatgtgg aaaggtattg tacagctgtg gcaatagtca
     2221 aggcttccct catttactaa cgtatatttt caaaaagaag atttattaaa cattccgtaa
     2281 agtacaagtt tttatatttt tcataccttt cctcttagag gttgttgcct ctaaaatatt
     2341 ttaaataagc agactgacgg ttttattgtg gttaatgtaa tgcaaaccta atataaacag
     2401 ttgggtgctt caacagggca ttctcagcag actggcaacg tgttttaata aaaacgttaa
     2461 tgttaactca gggcaggtaa cctggctcag ctggtgacac caggaccaat gacttgagct
     2521 ttgacccctg ggcaaccttt gtgagaacct ctaccccagc aagttgtcca caaacactct
     2581 gcagtgcgcg tacaccgtgc ccatccccca cacataaaaa accaaaagaa tgaaatgttt
     2641 ttaaatttaa aaacgaattc ttagtatgag actgaaaaat acaagtgttg tattatggta
     2701 aattgatgaa ttggaagtta cacagctttg agcctgatgt cacggtgaag ctttaatccc
     2761 agcactcagg aggcgtggcc atgtggagtt ctgtgagttc caggtcagcc aggactacac
     2821 aggacttcgg agtgggaccc tgtatgttta tatttttata tattcctatc taatgtcctg
     2881 taattcgtgt aaccaaaggt gatgttagat cctgctgccc gttagaacgg actaggctgt
     2941 acatggtatg tcttcatcat gaacagctca agcctgaata gaccatacag cctagaggtt
     3001 tcttttttct tttttctttt actggatatt ttatttattt acatttcaaa tgttatcccc
     3061 ttccccggtt tcccctccag aaacccccaa tcccaccatc cctccccctg cttctatgag
     3121 ggtgttcccc cacccaccca cccactccca tatccctgcc ctggcattct ggggcatcga
     3181 gccttcacag gaccaagggc ctctcctccc attgatgccc tacaaggctg tcctctgcta
     3241 catatgcggc tgcagccatg ggtcaatcta tactcttctc atagtgaaga gtaaaattta
     3301 tgaaaaagca agcaagttca agaaatggca gtcagtcttt tccttttgtt tggaaatgct
     3361 ggagaaatta tgcaggtttt aaaaatgccc ataatgttca ccatcttaat gcactcattt
     3421 tggattttat ttttatattg cattttcctc tttatttaca taattcaggc tcctctgctc
     3481 ttactgattc ttgagacgat tttatctggg cacatttagt cttcacgaat atatctgtta
     3541 tatctctgta ctcagttcat ttaaactact ttcccggggc tacacacata agcagccttt
     3601 ttttttttct aggacacatt cttgtaatgt ggttatttta ggggaagcaa taagagcttg
     3661 tgatcccctg ggctgtaaca tatagtacca ccattgtttg ggattttata cattgttgct
     3721 aaagtttccc tgcttttgtg tgacctacct tcacaccctg ttcatttgtc taaataaatg
     3781 tttctttgta tgtaa
//
