LOCUS       NM_153597               1168 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant
            1, mRNA.
ACCESSION   NM_153597
VERSION     NM_153597.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Yang,X., Wang,J., Li,S., Li,X., Gong,J., Yan,Z., Zhou,H., Wu,C. and
            Liu,X.
  TITLE     Structural and biochemical insights into the molecular mechanism of
            TRPT1 for nucleic acid ADP-ribosylation
  JOURNAL   Nucleic Acids Res 51 (14), 7649-7665 (2023)
   PUBMED   37334830
  REMARK    GeneRIF: Structural and biochemical insights into the molecular
            mechanism of TRPT1 for nucleic acid ADP-ribosylation.
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Harding,H.P., Lackey,J.G., Hsu,H.C., Zhang,Y., Deng,J., Xu,R.M.,
            Damha,M.J. and Ron,D.
  TITLE     An intact unfolded protein response in Trpt1 knockout mice reveals
            phylogenic divergence in pathways for RNA ligation
  JOURNAL   RNA 14 (2), 225-232 (2008)
   PUBMED   18094117
  REMARK    GeneRIF: Inactivation of the Trpt1 gene, encoding the only known
            mammalian homolog of Tpt1p, eliminates all detectable
            2'-phosphotransferase activity from cultured mouse cells but has no
            measurable effect on spliced Xbp-1 translation.
REFERENCE   3  (bases 1 to 1168)
  AUTHORS   Kuo,B.Y., Chen,Y., Bohacec,S., Johansson,O., Wasserman,W.W. and
            Simpson,E.M.
  TITLE     SAGE2Splice: unmapped SAGE tags reveal novel splice junctions
  JOURNAL   PLoS Comput Biol 2 (4), e34 (2006)
   PUBMED   16683015
REFERENCE   4  (bases 1 to 1168)
  AUTHORS   Tanaka,K., Tamura,H., Tanaka,H., Katoh,M., Futamata,Y., Seki,N.,
            Nishimune,Y. and Hara,T.
  TITLE     Spermatogonia-dependent expression of testicular genes in mice
  JOURNAL   Dev Biol 246 (2), 466-479 (2002)
   PUBMED   12051830
REFERENCE   5  (bases 1 to 1168)
  AUTHORS   Spinelli,S.L., Malik,H.S., Consaul,S.A. and Phizicky,E.M.
  TITLE     A functional homolog of a yeast tRNA splicing enzyme is conserved
            in higher eukaryotes and in Escherichia coli
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14136-14141 (1998)
   PUBMED   9826666
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK038830.1 and BC027575.1.
            
            On Mar 14, 2009 this sequence version replaced NM_153597.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and is protein-coding.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027575.1, SRR7652917.678266.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-202               AK038830.1         3-204
            203-1168            BC027575.1         1-966
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19"
     gene            1..1168
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /note="tRNA phosphotransferase 1"
                     /db_xref="GeneID:107328"
                     /db_xref="MGI:MGI:1333115"
     exon            1..394
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     CDS             335..1084
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /EC_number="2.7.1.160"
                     /note="mTPT1; EST-MNCb3719; tRNA splicing 2'
                     phosphotransferase 1 homolog"
                     /codon_start=1
                     /product="tRNA 2'-phosphotransferase 1"
                     /protein_id="NP_705825.2"
                     /db_xref="CCDS:CCDS50375.1"
                     /db_xref="GeneID:107328"
                     /db_xref="MGI:MGI:1333115"
                     /translation="MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLP
                     MRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHS
                     LQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGV
                     ISGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPT
                     RKPLSLAGDKETETQSGPKLSSRGGRRKIQQ"
     exon            395..476
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     exon            477..646
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     exon            647..821
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     exon            822..878
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     exon            879..989
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1144
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1080..1085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /note="hexamer: AATAAA"
     regulatory      1096..1101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /note="hexamer: ATTAAA"
     polyA_site      1115
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /note="major polyA site"
     polyA_site      1127
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
ORIGIN      
        1 ctggcttccc attagccagt ccgtgttcag gtccggatcc cgccctccgc agtggagccc
       61 ttcagatcta tgcagacccg ccgacctact tgtcaacgca tacgccagcc cggtgcccgg
      121 ggcagccgtg ctgtgatctg atttcagggc ccggggtctg gtccggatcc ctcgggcctc
      181 gaggctgcca gtgtgacctt gagcaagtct cgcccgctgg gcctcaagtt cccttgctga
      241 actagggtgg gatgagcagt gcggttctta tcctgagctt ttgtaacttt ctgggcagag
      301 aacccacact ccctgctccc tacaggtctt gatcatgaac gcccctggag gaaggaggaa
      361 ggaaggtaga aggactcata gaccccggga acaggaccga aatgtgcagc tgtctaaggc
      421 tctgtcctat gcccttcgcc acggggcctt gaagctggga cttcccatgc gagctgatgg
      481 ctttgtgccc ctgcaagccc tcctgcagct gccccagttc cacagctttt ccattgaaga
      541 tgtgcagctc gtggtcaata ccaacgaaaa gcagcggttt accctgcagc caggcgagcc
      601 gagcactggg ctcctcatca gggccaatca gggccactcc ctacaagtcc ctgagttgga
      661 actgacgccc ctggagacac cacaagccct gcccttgaca ctagtccatg gtacattctg
      721 gaagcactgg ccatctattc tactaaaggg cttgtcacgc caaggaagga cacacatcca
      781 cttggcctca ggattgcctg gggaccctgg tgtcatcagc ggcattcgtc caaattgtga
      841 ggtggcggtg ttcatcgatg gacccctagc cctcacagat ggaatccctt tcttctgctc
      901 cgccaatggt gtgattctga ctccagggaa tgctgaaggc ttcctgcttc ccaagtactt
      961 caaggaggcc ctgcagcttc gacctacacg aaagcctctc tccttggctg gtgataaaga
     1021 gacagagact cagagtggcc ccaagctcag ttccagagga ggaagaagaa agatccaaca
     1081 ataaaatatt tatttattaa aaaacctgaa acagacacag aaagaaagtc cagcttcaaa
     1141 ccagaaaaaa aaaaaaaaaa aaaaaaaa
//
