LOCUS       NP_705825                249 aa            linear   ROD 28-APR-2025
DEFINITION  tRNA 2'-phosphotransferase 1 [Mus musculus].
ACCESSION   NP_705825
VERSION     NP_705825.2
DBSOURCE    REFSEQ: accession NM_153597.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 249)
  AUTHORS   Yang,X., Wang,J., Li,S., Li,X., Gong,J., Yan,Z., Zhou,H., Wu,C. and
            Liu,X.
  TITLE     Structural and biochemical insights into the molecular mechanism of
            TRPT1 for nucleic acid ADP-ribosylation
  JOURNAL   Nucleic Acids Res 51 (14), 7649-7665 (2023)
   PUBMED   37334830
  REMARK    GeneRIF: Structural and biochemical insights into the molecular
            mechanism of TRPT1 for nucleic acid ADP-ribosylation.
REFERENCE   2  (residues 1 to 249)
  AUTHORS   Harding,H.P., Lackey,J.G., Hsu,H.C., Zhang,Y., Deng,J., Xu,R.M.,
            Damha,M.J. and Ron,D.
  TITLE     An intact unfolded protein response in Trpt1 knockout mice reveals
            phylogenic divergence in pathways for RNA ligation
  JOURNAL   RNA 14 (2), 225-232 (2008)
   PUBMED   18094117
  REMARK    GeneRIF: Inactivation of the Trpt1 gene, encoding the only known
            mammalian homolog of Tpt1p, eliminates all detectable
            2'-phosphotransferase activity from cultured mouse cells but has no
            measurable effect on spliced Xbp-1 translation.
REFERENCE   3  (residues 1 to 249)
  AUTHORS   Kuo,B.Y., Chen,Y., Bohacec,S., Johansson,O., Wasserman,W.W. and
            Simpson,E.M.
  TITLE     SAGE2Splice: unmapped SAGE tags reveal novel splice junctions
  JOURNAL   PLoS Comput Biol 2 (4), e34 (2006)
   PUBMED   16683015
REFERENCE   4  (residues 1 to 249)
  AUTHORS   Tanaka,K., Tamura,H., Tanaka,H., Katoh,M., Futamata,Y., Seki,N.,
            Nishimune,Y. and Hara,T.
  TITLE     Spermatogonia-dependent expression of testicular genes in mice
  JOURNAL   Dev Biol 246 (2), 466-479 (2002)
   PUBMED   12051830
REFERENCE   5  (residues 1 to 249)
  AUTHORS   Spinelli,S.L., Malik,H.S., Consaul,S.A. and Phizicky,E.M.
  TITLE     A functional homolog of a yeast tRNA splicing enzyme is conserved
            in higher eukaryotes and in Escherichia coli
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14136-14141 (1998)
   PUBMED   9826666
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK038830.1 and BC027575.1.
            
            On Mar 14, 2009 this sequence version replaced NP_705825.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and is protein-coding.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027575.1, SRR7652917.678266.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19"
     Protein         1..249
                     /product="tRNA 2'-phosphotransferase 1"
                     /EC_number="2.7.1.160"
                     /note="mTPT1; EST-MNCb3719; tRNA splicing 2'
                     phosphotransferase 1 homolog"
                     /calculated_mol_wt=27222
     Region          1..25
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K3A2.2)"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q86TN4; propagated from
                     UniProtKB/Swiss-Prot (Q8K3A2.2)"
     Region          26..198
                     /region_name="PTS_2-RNA"
                     /note="RNA 2'-phosphotransferase, Tpt1 / KptA family;
                     pfam01885"
                     /db_xref="CDD:460371"
     Region          220..249
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K3A2.2)"
     CDS             1..249
                     /gene="Trpt1"
                     /gene_synonym="Gm35838; Tpt1; Tpt1h"
                     /coded_by="NM_153597.2:335..1084"
                     /db_xref="CCDS:CCDS50375.1"
                     /db_xref="GeneID:107328"
                     /db_xref="MGI:MGI:1333115"
ORIGIN      
        1 mnapggrrke grrthrpreq drnvqlskal syalrhgalk lglpmradgf vplqallqlp
       61 qfhsfsiedv qlvvntnekq rftlqpgeps tglliranqg hslqvpelel tpletpqalp
      121 ltlvhgtfwk hwpsillkgl srqgrthihl asglpgdpgv isgirpncev avfidgplal
      181 tdgipffcsa ngviltpgna egfllpkyfk ealqlrptrk plslagdket etqsgpklss
      241 rggrrkiqq
//
