LOCUS       NM_021450               7145 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Mus musculus transient receptor potential cation channel, subfamily
            M, member 7 (Trpm7), transcript variant 1, mRNA.
ACCESSION   NM_021450
VERSION     NM_021450.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Akizawa,H., Lopes,E.M. and Fissore,R.A.
  TITLE     Zn2+ is essential for Ca2+ oscillations in mouse eggs
  JOURNAL   Elife 12, 88082 (2023)
   PUBMED   38099643
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7145)
  AUTHORS   Martinez-Gil,N., Kutsyr,O., Fernandez-Sanchez,L., Sanchez-Saez,X.,
            Albertos-Arranz,H., Sanchez-Castillo,C., Vidal-Gil,L., Cuenca,N.,
            Lax,P. and Maneu,V.
  TITLE     Ischemia-Reperfusion Increases TRPM7 Expression in Mouse Retinas
  JOURNAL   Int J Mol Sci 24 (22), 16068 (2023)
   PUBMED   38003256
  REMARK    GeneRIF: Ischemia-Reperfusion Increases TRPM7 Expression in Mouse
            Retinas.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7145)
  AUTHORS   Bai,Y., Bentley,L., Ma,C., Naveenan,N., Cleak,J., Wu,Y.,
            Simon,M.M., Westerberg,H., Canas,R.C., Horner,N., Pandey,R.,
            Paphiti,K., Schulze,U., Mianne,J., Hough,T., Teboul,L., de
            Baaij,J.H.F. and Cox,R.D.
  TITLE     Cleft palate and minor metabolic disturbances in a mouse global
            Arl15 gene knockout
  JOURNAL   FASEB J 37 (11), e23211 (2023)
   PUBMED   37773757
REFERENCE   5  (bases 1 to 7145)
  AUTHORS   Gupta,N., Soriano-Ubeda,C., Stein,P., Savy,V., Papas,B.N.,
            Ardestani,G., Carvacho,I., Alfandari,D., Williams,C.J. and
            Fissore,R.A.
  TITLE     Essential role of Mg2+ in mouse preimplantation embryo development
            revealed by TRPM7 chanzyme-deficient gametes
  JOURNAL   Cell Rep 42 (10), 113232 (2023)
   PUBMED   37824328
  REMARK    GeneRIF: Essential role of Mg[2+] in mouse preimplantation embryo
            development revealed by TRPM7 chanzyme-deficient gametes.
REFERENCE   6  (bases 1 to 7145)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7145)
  AUTHORS   Monteilh-Zoller,M.K., Hermosura,M.C., Nadler,M.J.,
            Scharenberg,A.M., Penner,R. and Fleig,A.
  TITLE     TRPM7 provides an ion channel mechanism for cellular entry of trace
            metal ions
  JOURNAL   J Gen Physiol 121 (1), 49-60 (2003)
   PUBMED   12508053
  REMARK    GeneRIF: The Trpm7 ion channel possesses the unique ability to
            efficiently conduct many essential trace metal ions such as Zn2+,
            Fe2+, Cu2+, Mn2+, and Co2+ into cells, along with several toxic
            metal ions such as Cd2+, Ba2+, and Sr2+ as well.
REFERENCE   8  (bases 1 to 7145)
  AUTHORS   Nadler,M.J., Hermosura,M.C., Inabe,K., Perraud,A.L., Zhu,Q.,
            Stokes,A.J., Kurosaki,T., Kinet,J.P., Penner,R., Scharenberg,A.M.
            and Fleig,A.
  TITLE     LTRPC7 is a Mg.ATP-regulated divalent cation channel required for
            cell viability
  JOURNAL   Nature 411 (6837), 590-595 (2001)
   PUBMED   11385574
  REMARK    Erratum:[Nature 2001 Aug 9;412(6847):660]
REFERENCE   9  (bases 1 to 7145)
  AUTHORS   Yamaguchi,H., Matsushita,M., Nairn,A.C. and Kuriyan,J.
  TITLE     Crystal structure of the atypical protein kinase domain of a TRP
            channel with phosphotransferase activity
  JOURNAL   Mol Cell 7 (5), 1047-1057 (2001)
   PUBMED   11389851
REFERENCE   10 (bases 1 to 7145)
  AUTHORS   Runnels,L.W., Yue,L. and Clapham,D.E.
  TITLE     TRP-PLIK, a bifunctional protein with kinase and ion channel
            activities
  JOURNAL   Science 291 (5506), 1043-1047 (2001)
   PUBMED   11161216
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732330.14.
            
            On Aug 28, 2009 this sequence version replaced NM_021450.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY032951.1, AF149013.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-288               AL732330.14        184824-185111       c
            289-368             AL732330.14        167263-167342       c
            369-407             AL732330.14        163493-163531       c
            408-606             AL732330.14        160193-160391       c
            607-820             AL732330.14        158749-158962       c
            821-945             AL732330.14        157347-157471       c
            946-1117            AL732330.14        154924-155095       c
            1118-1292           AL732330.14        153150-153324       c
            1293-1416           AL732330.14        151706-151829       c
            1417-1489           AL732330.14        149594-149666       c
            1490-1590           AL732330.14        146185-146285       c
            1591-1725           AL732330.14        144306-144440       c
            1726-1779           AL732330.14        142538-142591       c
            1780-1920           AL732330.14        140047-140187       c
            1921-2055           AL732330.14        138979-139113       c
            2056-2336           AL732330.14        137860-138140       c
            2337-2565           AL732330.14        135565-135793       c
            2566-2721           AL732330.14        134484-134639       c
            2722-2865           AL732330.14        134262-134405       c
            2866-2994           AL732330.14        132826-132954       c
            2995-3273           AL732330.14        131320-131598       c
            3274-3448           AL732330.14        130014-130188       c
            3449-3575           AL732330.14        128687-128813       c
            3576-3760           AL732330.14        125610-125794       c
            3761-3893           AL732330.14        123538-123670       c
            3894-4606           AL732330.14        121546-122258       c
            4607-4674           AL732330.14        119013-119080       c
            4675-4765           AL732330.14        116139-116229       c
            4766-4836           AL732330.14        113867-113937       c
            4837-4871           AL732330.14        111436-111470       c
            4872-4897           AL732330.14        109973-109998       c
            4898-4948           AL732330.14        108163-108213       c
            4949-5014           AL732330.14        108019-108084       c
            5015-5298           AL732330.14        106512-106795       c
            5299-5381           AL732330.14        106317-106399       c
            5382-5587           AL732330.14        105961-106166       c
            5588-5639           AL732330.14        104293-104344       c
            5640-5746           AL732330.14        104082-104188       c
            5747-7145           AL732330.14        100408-101806       c
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.76 cM"
     gene            1..7145
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /note="transient receptor potential cation channel,
                     subfamily M, member 7"
                     /db_xref="GeneID:58800"
                     /db_xref="MGI:MGI:1929996"
     exon            1..288
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            289..368
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            369..407
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            408..606
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            607..820
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            821..945
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1117
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1292
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1416
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1489
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1590
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1725
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1779
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1920
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2055
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2336
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2337..2565
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2721
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2865
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..2994
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3273
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3274..3448
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3575
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3576..3760
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3761..3893
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4606
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4607..4674
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4675..4765
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4766..4836
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4837..4871
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4872..4897
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4898..4948
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4949..5014
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5015..5298
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5299..5381
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5382..5587
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5588..5639
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5640..5746
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     exon            5747..7145
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7117..7122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /note="hexamer: ATTAAA"
     polyA_site      7136
                     /gene="Trpm7"
                     /gene_synonym="2310022G15Rik; 4833414K03Rik;
                     5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7;
                     TRPPLIK"
                     /note="major polyA site"
ORIGIN      
        1 cactcgacgg ccccgtcacg tggctgccgc ggccccgtct ccggcggagg cgggcgcggg
       61 cgcgtccctg tggccagtca cccggcggag ctggtcgcac aattatgaaa gactcgactt
      121 ctgctgctag cgctggagct gagttagttc tgagaaggtt tcccggggct gtccttgttc
      181 ggtggcccgt gccaccgcct ccggagacgc tttccgatag atggctgcag gccgcggagg
      241 tggaggagga gccgctgccc ttccggagtc cgccccgtga ggagaatgtc ccagaaatcc
      301 tggatagaga gcactttgac caagagggag tgtgtatata ttataccaag ctccaaagac
      361 cctcacagat gtcttccagg atgtcagatt tgtcagcaac ttgtcagatg tttctgtggt
      421 cgtttggtca agcaacatgc atgctttact gcaagtcttg ccatgaaata ctcagatgtg
      481 aaattgggtg aacactttaa ccaggcaata gaagaatggt ctgtggaaaa gcacacggag
      541 cagagcccaa cagatgctta tggagtcatc aattttcaag ggggttctca ttcctacaga
      601 gctaagtatg tgagactatc atatgatacc aaacctgaaa tcattctgca acttctgctt
      661 aaagaatggc aaatggagtt acccaaactt gttatttctg tacatggagg catgcagaag
      721 tttgaacttc atccaagaat caagcagttg cttggaaagg gtcttattaa agctgcagtt
      781 acaaccggag cttggatttt aactggagga gtcaatacag gtgtggcaaa acatgttggt
      841 gatgccctca aagaacatgc ttccagatca tctcgaaaaa tttgcactat tggaatagct
      901 ccatggggag tgatagaaaa cagaaatgat cttgttggga gagatgtggt tgctccttat
      961 caaaccctat tgaatccctt gagcaaattg aatgttctga ataatctaca ctcccatttc
     1021 atcttggtgg atgatggcac tgttggaaag tatggggcag aagtcagact gagaagagaa
     1081 cttgaaaaaa ccattaatca gcaaagaatt catgctagaa ttgggcaagg agttcctgtg
     1141 gtggctttga tatttgaagg cgggccaaat gtcatcctta cagtactgga gtaccttcag
     1201 gaaagccccc cagttccagt tgttgtgtgt gaagggacag gcagagctgc agatttacta
     1261 gcctatatcc acaaacagac agaggaagga ggaaatcttc ctgatgcagc agagcctgat
     1321 attatatcaa ctatcaagaa aacatttaac tttggccaga gtgaagcagt tcatttattt
     1381 caaacaatga tggagtgtat gaaaaaaaaa gagcttatca ctgtttttca cattggatca
     1441 gaggatcatc aagatataga tgtggccata ctcactgcac tgctgaaagg tactaatgca
     1501 tctgcatttg accagcttat ccttacactg gcatgggaca gagttgatat tgccaaaaat
     1561 catgtatttg tttatggaca acagtggctg gttggatcct tggaacaggc tatgcttgat
     1621 gctcttgtaa tggacagagt ttcatttgta aaacttctta ttgaaaacgg agtaagcatg
     1681 cataaattcc ttaccattcc cagactggaa gaactttata acactaaaca aggtccaacc
     1741 aatccaatgt tgttccatct cattcgggat gtcaagcagg gtaatctccc cccggggtac
     1801 aagatcactt taattgatat aggacttgtg attgagtatc tcatgggagg aacctacaga
     1861 tgcacataca cacgaaaacg ttttcgattg atatataata gtcttggtgg aaataaccgg
     1921 aggtcaggtc gaaatacctc cagcagcacc cctcagttgc gaaagagtca tgaaactttt
     1981 ggcaatagag ctgataaaaa ggaaaaaatg agacacaatc atttcattaa aacagcccaa
     2041 ccctacagac caaagatgga tgcatctatg gaagaaggaa agaagaaaag aaccaaagat
     2101 gaaattgtag atatagatga tccagagacc aagcgctttc cttatcctct taatgaatta
     2161 ttaatttggg cttgccttat gaagaggcag gtcatggccc gctttttatg gcagcatggt
     2221 gaagaatcaa tggctaaagc attagttgcc tgtaaaatct atcgttcaat ggcttatgag
     2281 gcaaagcaga gtgacctggt agatgatact tcagaggaac tgaagcagta ttccaatgat
     2341 tttggccaac tggcagttga attactggaa cagtccttca gacaggatga aacgatggct
     2401 atgaaattac tcacttatga actcaaaaac tggagtaatt caacctgcct caagttagca
     2461 gtttcttcaa gacttagacc ttttgtagct cacacttgta cacagatgtt gttatctgat
     2521 atgtggatgg gacggctgaa tatgagaaaa aattcctggt ataaggtcat attaagcatt
     2581 ttagttccac ctgccatatt aatgctagag tataaaacca aggctgaaat gtcccatatc
     2641 ccacaatctc aagatgctca tcaaatgacg atggaggata gtgaaaacaa ttttcacaac
     2701 ataacagaag agatacccat ggaagtattt aaagaagtaa agattttgga cagcagtgat
     2761 ggaaagaatg aaatggagat acatattaaa tcaaaaaagc ttccaatcac acgaaaattt
     2821 tatgcctttt atcatgcacc aattgtaaag ttctggttta acacattggc atatttagga
     2881 tttctgatgc tttatacatt tgtagttctt gtaaaaatgg aacagttacc ttcagttcaa
     2941 gaatggattg ttatcgctta tatttttacc tatgctattg aaaaagtccg tgaggtcttc
     3001 atgtctgaag ctgggaaaat cagccagaag attaaagtat ggtttagtga ctacttcaat
     3061 gtcagtgaca caattgccat catttctttc tttgttggat ttggactaag atttggagca
     3121 aaatggaact atattaatgc atatgataat catgtttttg tggctggaag attaatttac
     3181 tgtcttaata taatattttg gtatgtgcgt ttgctagact ttctagccgt aaatcaacag
     3241 gcaggacctt atgtaatgat gattggaaaa atggtggcca atatgttcta cattgtagtg
     3301 ataatggctc ttgtattgct tagttttggt gttcccagaa aagcaatact ttatccacat
     3361 gaagaaccat cttggtctct tgctaaagat atagtttttc atccatactg gatgattttt
     3421 ggtgaagttt atgcatatga aattgatgtg tgtgcaaatg actccactct cccgacaatc
     3481 tgtggtcctg gaacttggtt gactccattt cttcaagcag tctacctctt tgtacagtat
     3541 atcattatgg ttaatctcct tatcgcattt ttcaataatg tatatttaca agtgaaggca
     3601 atttccaata ttgtatggaa gtatcagcgg tatcatttta ttatggctta tcatgaaaaa
     3661 ccagtcctgc ctcctcctct tatcatcctc agccatatag tttcactgtt ttgctgtgta
     3721 tgcaaaagaa gaaagaaaga taagacttcc gatgggccaa aacttttctt aacagaagaa
     3781 gatcaaaaga aactccatga ttttgaagag cagtgtgttg agatgtactt tgatgagaaa
     3841 gatgacaaat tcaattctgg gagtgaagag agaatccggg tcacttttga aagagtggag
     3901 cagatgagca ttcagattaa agaagttgga gatcgtgtca actacataaa aagatcatta
     3961 cagtctttag attctcaaat tggtcatctg caagatctct cagccctaac agtagataca
     4021 ttgaaaacac ttacagccca gaaagcttca gaagctagta aagtgcacaa tgagatcaca
     4081 cgagaattga gtatttccaa acacttggct cagaatctta ttgatgatgt tcctgtaaga
     4141 cctttgtgga agaaacctag tgctgtaaac acactgagtt cctctcttcc tcaaggtgat
     4201 cgggaaagta ataatccttt tctttgtaat atttttatga aagatgaaaa agacccccaa
     4261 tataatctgt ttggacaaga tttgcccgtg ataccccaga gaaaagaatt caacattcca
     4321 gaggctggtt cctcctgtgg tgccttattc ccaagtgctg tttctccccc agaattacga
     4381 cagagacgac atggggtaga aatgttaaaa atatttaata aaaatcaaaa attaggcagt
     4441 tcacctaata gttcaccaca tatgtcctcc ccaccaacca aattttctgt gagtacccca
     4501 tcccagccaa gttgcaaaag tcacttggaa tccacaacca aagatcaaga acccattttc
     4561 tataaagctg cagaagggga taacatagaa tttggagcat ttgtgggaca cagagatagt
     4621 atggacttac agaggtttaa agaaacatca aacaaaataa gagaactgtt atctaatgat
     4681 actcctgaaa acactctgaa acatgtgggt gctgctggat atagtgaatg ttgtaagact
     4741 tctacttctc ttcactcggt gcaagcagaa agctgtagta gaagagcgtc gacggaagac
     4801 tctccagaag tcgattctaa agcagctttg ttaccggatt ggttacgaga tagaccatca
     4861 aacagagaaa tgccatctga aggaggaaca ttaaatggtc ttgcttctcc atttaagccc
     4921 gttttggata caaattacta ttattcagct gtggaaagaa ataacctgat gaggttgtca
     4981 cagagtattc ccttcgttcc tgtacctcca cgaggcgagc ctgtcacagt gtaccgtctg
     5041 gaggagagtt ctcccagtat actgaataac agcatgtctt catggtctca gctaggcctc
     5101 tgtgccaaaa ttgagttttt aagtaaagag gaaatgggag gtggtttacg aagagcagtc
     5161 aaagtgctgt gtacctggtc agagcacgat atcctgaagt cagggcatct ctatatcatt
     5221 aagtcatttc ttcctgaggt gataaacaca tggtcaagca tttataaaga agatacggtt
     5281 ctacatctct gtctcagaga aatacaacaa cagagagcag cacaaaagct cacatttgcc
     5341 tttaatcaga tgaaacccaa atccatacca tattctccaa ggttccttga agttttcctg
     5401 ttgtactgcc attcagcagg gcagtggttt gctgtagaag agtgcatgac tggtgaattt
     5461 agaaaataca acaacaataa tggtgatgaa atcattccta caaatactct agaagagatc
     5521 atgctagcct ttagccactg gacctatgaa tataccagag gggagttact ggtacttgac
     5581 ttacaaggag tgggagaaaa cttgactgac ccatctgtaa taaaagctga agaaaaaaga
     5641 tcctgtgaca tggtttttgg ccctgccaat ctaggagaag atgcaataaa aaacttcaga
     5701 gccaaacatc actgtaattc ttgctgtcga aagcttaaac ttccagattt gaagaggaat
     5761 gactacacgc ctgataaaat tatatttcct caggatgagt catcagattt gaatcttcaa
     5821 tctggaaatt ccaccaaaga atcagaagca acaaattctg ttcgtctgat gttatagtgc
     5881 tgagtcattg gtttttgcct acacttcaca aaagtgtaac tgtcagtttt cctttcgggg
     5941 gaattgatga tataggaaga tgtgtgcaaa atgagcttgc tggccccaca catagtctag
     6001 aggtaatgtt ctcattgaaa aacgcctgga ggctgcagat gacagctgga aagtgctagc
     6061 tggcagagag tcagtgctct cggctggtga agggcgggaa ccttgctgct gagagtggtg
     6121 gttctctcac ctggtgcagg accattaacc aaagtcaagt cttcagattt gattggctgc
     6181 tcagtcacag ccattcagct aaggaaacta aattgcgcag ctttttaaat ggctgaagtc
     6241 ttcctcagtt tgtgctctat gataatgatg ttagctctca actaggtgtt tgtggccacg
     6301 ggagaactac tccttacaat tttgcttcac aggcatgtta caaagcctgc actgaaaacc
     6361 gtttgtcttc cctctctccc tccctctttt ccctgtagta ttgaggatca aacccagggc
     6421 ctcatgaaga ccattttcta agagacattt tatttaagaa tcaactatag agtctatgtt
     6481 tatggataca gccagttttt gttaaacaaa acctgaattg tgcaaaaggg ttttttaaca
     6541 tttatcaatg ttaagtaaaa gaaagccatg ataaataaga attaactcac tgttcaatgg
     6601 gtgtttcctg tgaggaaggt tacagttgta acagcctgca gttgcataca tctccaaaga
     6661 tttacagact tagtgtatca aatcagagtg tcatgtgagc tctcacattg aaaattctat
     6721 aggaatgtgt caatgtgaat tctatttctg gtacttaaga aatcagttgt tggattatcc
     6781 ttatacagta tagggagatc acaatacaac tttatgccaa taaaatctaa cttaattgcc
     6841 cagatatttt tgcatattta gcaacaagaa aagcttatca tttgactcaa gttttatgct
     6901 ttctctttct tttcatttcc taggtactaa ttttaatttt tatttggaag gagcagtgta
     6961 aagcttactt gtattcaata gtgtatctca tagatacaga caaggccgca gagataagct
     7021 gttaaatagt gtttaatgtt gatgtggaga gaaaggtgta ttacttaaaa atactatacc
     7081 atatacgttt tgtatatcat taaatcttta aaagaaatta aatttattct tgtttacaga
     7141 tgttt
//
