LOCUS       NM_153417               6762 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transient receptor potential cation channel, subfamily
            M, member 6 (Trpm6), transcript variant 1, mRNA.
ACCESSION   NM_153417 XM_911536
VERSION     NM_153417.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Adella,A., Gommers,L.M.M., Bos,C., Leermakers,P.A., de Baaij,J.H.F.
            and Hoenderop,J.G.J.
  TITLE     Characterization of intestine-specific TRPM6 knockout C57BL/6 J
            mice: effects of short-term omeprazole treatment
  JOURNAL   Pflugers Arch 477 (1), 99-109 (2025)
   PUBMED   39266724
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6762)
  AUTHORS   Funato,Y., Yamazaki,D., Okuzaki,D., Yamamoto,N. and Miki,H.
  TITLE     Importance of the renal ion channel TRPM6 in the circadian
            secretion of renin to raise blood pressure
  JOURNAL   Nat Commun 12 (1), 3683 (2021)
   PUBMED   34140503
  REMARK    GeneRIF: Importance of the renal ion channel TRPM6 in the circadian
            secretion of renin to raise blood pressure.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6762)
  AUTHORS   De Clercq,K., Perez-Garcia,V., Van Bree,R., Pollastro,F.,
            Peeraer,K., Voets,T. and Vriens,J.
  TITLE     Mapping the expression of transient receptor potential channels
            across murine placental development
  JOURNAL   Cell Mol Life Sci 78 (11), 4993-5014 (2021)
   PUBMED   33884443
REFERENCE   5  (bases 1 to 6762)
  AUTHORS   Franken,G.A.C., Seker,M., Bos,C., Siemons,L.A.H., van der
            Eerden,B.C.J., Christ,A., Hoenderop,J.G.J., Bindels,R.J.M.,
            Muller,D., Breiderhoff,T. and de Baaij,J.H.F.
  TITLE     Cyclin M2 (CNNM2) knockout mice show mild hypomagnesaemia and
            developmental defects
  JOURNAL   Sci Rep 11 (1), 8217 (2021)
   PUBMED   33859252
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6762)
  AUTHORS   Rondon,L.J., Groenestege,W.M., Rayssiguier,Y. and Mazur,A.
  TITLE     Relationship between low magnesium status and TRPM6 expression in
            the kidney and large intestine
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 294 (6), R2001-R2007 (2008)
   PUBMED   18385471
  REMARK    GeneRIF: In C57BL/6J mice, dietary Mg(2+)-restriction results in
            increased Mg(2+) (re)absorption, which is correlated with increased
            TRPM6 expression.
REFERENCE   7  (bases 1 to 6762)
  AUTHORS   Cao,G., Thebault,S., van der Wijst,J., van der Kemp,A.,
            Lasonder,E., Bindels,R.J. and Hoenderop,J.G.
  TITLE     RACK1 inhibits TRPM6 activity via phosphorylation of the fused
            alpha-kinase domain
  JOURNAL   Curr Biol 18 (3), 168-176 (2008)
   PUBMED   18258429
REFERENCE   8  (bases 1 to 6762)
  AUTHORS   Ramsey,I.S., Delling,M. and Clapham,D.E.
  TITLE     An introduction to TRP channels
  JOURNAL   Annu Rev Physiol 68, 619-647 (2006)
   PUBMED   16460286
  REMARK    Review article
REFERENCE   9  (bases 1 to 6762)
  AUTHORS   Voets,T., Nilius,B., Hoefs,S., van der Kemp,A.W., Droogmans,G.,
            Bindels,R.J. and Hoenderop,J.G.
  TITLE     TRPM6 forms the Mg2+ influx channel involved in intestinal and
            renal Mg2+ absorption
  JOURNAL   J Biol Chem 279 (1), 19-25 (2004)
   PUBMED   14576148
REFERENCE   10 (bases 1 to 6762)
  AUTHORS   Ryazanov,A.G., Pavur,K.S. and Dorovkov,M.V.
  TITLE     Alpha-kinases: a new class of protein kinases with a novel
            catalytic domain
  JOURNAL   Curr Biol 9 (2), R43-R45 (1999)
   PUBMED   10021370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134906.3 and AC140458.3.
            
            On Jul 25, 2018 this sequence version replaced NM_153417.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY135644.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-271               AC134906.3         1-271               c
            272-313             AC140458.3         7-48
            314-387             AC140458.3         22327-22400
            388-426             AC140458.3         26699-26737
            427-604             AC140458.3         28526-28703
            605-818             AC140458.3         33046-33259
            819-943             AC140458.3         33780-33904
            944-1115            AC140458.3         36680-36851
            1116-1284           AC140458.3         41992-42160
            1285-1408           AC140458.3         42842-42965
            1409-1481           AC140458.3         46259-46331
            1482-1582           AC140458.3         46447-46547
            1583-1717           AC140458.3         50941-51075
            1718-1771           AC140458.3         52155-52208
            1772-1912           AC140458.3         59538-59678
            1913-2047           AC140458.3         61685-61819
            2048-2328           AC140458.3         63355-63635
            2329-2557           AC140458.3         65247-65475
            2558-2710           AC140458.3         69044-69196
            2711-2857           AC140458.3         73339-73485
            2858-2986           AC140458.3         75757-75885
            2987-3238           AC140458.3         77377-77628
            3239-3413           AC140458.3         79837-80011
            3414-3528           AC140458.3         80211-80325
            3529-3722           AC140458.3         82475-82668
            3723-3855           AC140458.3         87979-88111
            3856-4997           AC140458.3         103333-104474
            4998-5107           AC140458.3         106161-106270
            5108-5225           AC140458.3         112177-112294
            5226-5317           AC140458.3         116204-116295
            5318-5376           AC140458.3         117960-118018
            5377-5402           AC140458.3         120506-120531
            5403-5453           AC140458.3         122218-122268
            5454-5519           AC140458.3         123835-123900
            5520-5806           AC140458.3         125842-126128
            5807-5889           AC140458.3         126759-126841
            5890-6095           AC140458.3         127573-127778
            6096-6147           AC140458.3         133906-133957
            6148-6254           AC140458.3         138941-139047
            6255-6762           AC140458.3         142010-142517
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 13.21 cM"
     gene            1..6762
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /note="transient receptor potential cation channel,
                     subfamily M, member 6"
                     /db_xref="GeneID:225997"
                     /db_xref="MGI:MGI:2675603"
     exon            1..313
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..6388
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /EC_number="2.7.11.1"
                     /note="transient receptor potential cation channel
                     6/channel-kinase 2; melastatin-related TRP cation channel
                     6; putative cation channel subfamily M member 6"
                     /codon_start=1
                     /product="transient receptor potential cation channel
                     subfamily M member 6"
                     /protein_id="NP_700466.1"
                     /db_xref="CCDS:CCDS29691.1"
                     /db_xref="GeneID:225997"
                     /db_xref="MGI:MGI:2675603"
                     /translation="MQVKKSWIEGVFYKRECNKFIPSSKDPHRCTPGCQICHNLVRCY
                     CGRLIEEHHGLDRAWNLSVTEGHGDEQWSVEKHTVKSPTDTFGTINFQDGEHIHHSKY
                     IRTSWDTKSDHLLHLMLKEWNMELPKLVISVHGGLQNFKISSKLKETFSQGLVKAAET
                     TGAWIITEGINSGVSKHVGDALKAHSSKSLRKIWTVGIPPWGVIENQRELVGKDVVCM
                     YQTLGNPLSKLTTLNCMHSHFILCDDGTVGMYGNEEKLRRNLEKHLSMQKIHTCSRQG
                     VPVVGLVMEGGPNVILWVWETVKNKEPVVVCEGTGRAADLLAFTYKHLEDGGILRPQV
                     KEELFCLIQNMFNFSLRQSKHLFQILMECMVHKDSITIFDADSEEHQDLDLAILTALL
                     KGTSLSISEQLNLAMAWDRMDIAKKHILTYGQHWKPGALEQAMLDALVMDRVDFVKLL
                     IENGVNLHRFLTIPRLEELYNTKQGPTNKFLRHLVQDVKQHTLLSSYRITLIDIGLVI
                     EYLIGGAYRSSYTRKSFRILYNNLYRKHKSVSSFAQGLSQHSLHQRHSLRNRKESSES
                     TLHSQFFRTAQPYKSKEKPEDSQKSKKKSKERQSLSEEPEAAGFIYPYNDLLVWAVLM
                     KRQNMAMFFWQHGEEATVKAVIASILYRAMAREAKESNMVDDTSEELKNYSEQFGQLA
                     LDVLEKAFKQNEPMAMKLLTYELKNWSNSTCLKLAVSGGLRPFVSHSCTQMLLTDMWM
                     GRLKMRKNSWLKIIISILLPPMILTLEFKSKAEMSHVPQSQDFQFTWNYSDQGLSNTK
                     ESACVKDYDLERGPDEKPDEPLHLDLRNVPQSLPWTRRVYEFYSAPFVKFWFYTMAYL
                     AFLMLFTYTVLVEMQPQPSVHEWLVIIYIFTNAIEKVREICISEPSKFKQKVKMWLSE
                     YWNLMETVAIGLFAVGFGLRWGHPPLQTAGRLIYCIDIIFWFSRLMDFFAVNQHAGPY
                     VTMIAKMAANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWRLARDIVFEPYWMMYGE
                     VYASDIDVCSNETSCPPGSFLTPFLQAVYLFVQYIIMVNLLIACFNNIYLDIKSISNK
                     LWKYNRYRYIMTYHQKPWLPPPFILLNHLCLLLRGLCCRPAPQDQEEGDGGLKLYLTK
                     DDLKKLHDFEEQCVEKYFHEKTEGLNCSFEEQIRMTSERVSEMFFQLKEMNEKVSFIK
                     DSLLSLDSQVGHLQDLSAITVDTLKVLSAVDTLQEDEILLANRKHSTCRKRPHSWTNV
                     ICAKVLSDMESCGKKKLQYYSMPPSLLRSLARSQLPPSVQRGALVEVTHSKREASHVR
                     EEQEEREMEQRTTASGISHVRQAHSKYGQFLLVPSSGKQVPLSLETPPHLFRSSEEAG
                     IDGLVLEHIHQSDLTTHLPQQTPAASHQALVAEHKDQHEAVTQMSDKPAKAEQDLLAF
                     SGTPAPMTVTSLPSRAISMQDEGGYVNWAFSENDETGVFSFKKKWKTCLASTCNSDSN
                     PGGDYFLHTGGRSGLDNSRRLAQSCECPAGPWTQARRSFWINPLCRDKALIKSHSFRF
                     HKEEKLRKTWKNNSHSKSLETRSTWLKAKLLTKTRSLSKKKRKTQGLQVPVITVNACY
                     QSDQLNAEPGETNTTEEFSKKWLSVSNFSQIGLEPYIYQKMKMKEIKRHTTQASDHLR
                     QPQENRDKTPTWNSGSTSLSRSFLTRSPNEVHKISTSLKSPQEPHHHYSAIERNNLMR
                     LSQTIPFTPIQLFTGEEVTIYKLEESSPLTLDKSMSSWSQHGRAAMIQVLSQEEMDGG
                     LRKAMRVISTWSEDDVLKPGQVFIVKSFLPEVVQTWYKIFQESTVLHLCLREIQQQRA
                     AQKLIYTFNQVKPQTIPYTPRFLEVSLVYCHSANQWLTIEKYMTGEFRKYNNNNGDEI
                     APTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPA
                     NLGEDAIRSFIAKHRCNSCCGKLRLPDLKRNDYSLSRTHCNLGFGQTIEPTEELPERD
                     KNRSSLEDHTRL"
     exon            314..387
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            388..426
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            427..604
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            605..818
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            819..943
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1115
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1284
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1408
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1481
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1582
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1717
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1771
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1912
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2047
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2328
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2557
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2558..2710
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2857
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..2986
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3238
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3413
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3414..3528
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3529..3722
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..3855
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4997
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            4998..5107
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5108..5225
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5226..5317
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5318..5376
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5377..5402
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5403..5453
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5454..5519
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5520..5806
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5807..5889
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            5890..6095
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            6096..6147
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            6148..6254
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     exon            6255..6762
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6741..6746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /note="hexamer: AATAAA"
     polyA_site      6762
                     /gene="Trpm6"
                     /gene_synonym="CHAK2; TRPM6/CHAK2"
                     /note="major polyA site"
ORIGIN      
        1 aggatttgcg aaatgctagc tttcaagtcc tctagtctcc acccgaacat gattatcgta
       61 gctgaggttt tggcaaccgc gtggaccctt ccgaagggca ggacccgcag tctttcttcc
      121 gggagttggg accggcctgg agctgggtca gggccgagcg gaaaccgaac ccagggtcga
      181 ggcggcaggg cgcgcgtgtg agtcccggac tccggagtcc gagtccagga ggaatcgagt
      241 cctgggaggt gtcccggacg aggaggaatt cgctgtcgct tcggctcctg tggtggtgag
      301 aatgcaggtc aagaaatcct ggatcgaagg ggtattctac aagagagagt gtaacaaatt
      361 catacccagc tcaaaagacc ctcacagatg cactccaggc tgccaaatct gccacaattt
      421 agtcaggtgt tactgtggcc ggctgattga agaacaccat gggctggatc gtgcctggaa
      481 cctctcagtc actgagggcc atggggatga gcagtggtct gtggagaagc acacagtgaa
      541 aagccctaca gataccttcg gcaccattaa tttccaagat ggagaacata tccaccactc
      601 caagtacatt agaacttctt gggatacaaa atcggatcat ctgttgcact taatgctgaa
      661 agagtggaat atggaactgc caaagcttgt gatctccgtc catgggggtc ttcagaactt
      721 caagatatcc tccaagctca aagagacctt cagccagggt ctggtcaaag ctgcagagac
      781 cacaggagca tggataatta cagaaggcat aaactcagga gtgtccaagc acgttgggga
      841 tgccctaaaa gcccattcct ctaagtcttt gaggaaaatc tggacagttg gaatccctcc
      901 ttggggagtc attgagaacc agagagagct ggtcggaaaa gatgtggtgt gtatgtacca
      961 gacactgggt aacccactca gcaagctcac cacgctaaac tgtatgcact ctcacttcat
     1021 cctgtgtgat gatgggactg tgggcatgta tggaaatgaa gagaagctca ggcggaacct
     1081 ggagaagcat ctctcaatgc agaagataca cacttgctca agacaaggtg tgcctgtggt
     1141 ggggctggtg atggaaggag gtcccaatgt catcctctgg gtatgggaga ccgtcaagaa
     1201 caaggagccc gtggtggtgt gcgaggggac aggcagagct gctgacctct tggccttcac
     1261 ttacaaacat ttggaggatg gagggattct acgccctcag gtgaaagaag agctcttctg
     1321 tttgattcag aacatgttca actttagcct tagacagtct aagcaccttt tccaaattct
     1381 aatggagtgc atggtacaca aggattctat caccatattt gatgctgatt ctgaagagca
     1441 ccaagatctt gatttggcca tcctcacggc tctactgaag ggtacaagct tatcaatatc
     1501 agaacaatta aatctggcaa tggcttggga tagaatggac attgccaaga aacatatcct
     1561 aacttatgga caacattgga agcctggtgc tctagaacaa gcaatgctag atgctttagt
     1621 gatggatcga gtggactttg tgaaactttt gatagagaat ggggtgaacc tccaccgctt
     1681 cctcaccatc cctcggctgg aagagctcta taacacaaaa caaggaccaa ctaataaatt
     1741 tttgcgtcat cttgtccaag acgtaaagca gcacaccctt ctctcgagct atagaataac
     1801 actcattgac attgggctgg taatagagta cctcattggt ggagcatatc ggagcagcta
     1861 cactaggaaa agtttcagaa ttctctacaa caacctctac agaaaacaca agagtgtgag
     1921 cagctttgct cagggcctct cccagcactc tcttcatcag agacattcac tgagaaatag
     1981 aaaggagtct tctgaaagca cactgcactc ccagttcttc aggacggccc agccctacaa
     2041 atccaaggag aagcctgagg actctcaaaa atcaaagaag aagtccaagg aaagacaaag
     2101 cctgtcagag gaacccgagg ctgctggctt tatctaccca tacaatgacc ttctggtgtg
     2161 ggcggtgctc atgaagaggc agaatatggc catgttcttc tggcagcatg gagaggaggc
     2221 cacagtcaag gcggtcattg ctagtatcct ctacagagcc atggcccggg aagccaagga
     2281 gagcaacatg gtggatgaca cttcagaaga gttgaaaaat tactcagagc agtttggcca
     2341 gctcgccctg gatgtattag agaaagcctt caagcagaac gagccgatgg ccatgaagct
     2401 gctgacctat gagctcaaga attggagtaa ttccacctgc ttgaaactgg cggtctctgg
     2461 aggtctgcgc ccttttgtgt cgcattcttg tacccaaatg ctgctaacag acatgtggat
     2521 ggggcgtctg aaaatgagaa aaaattcctg gctgaagata atcatcagta tcctcttacc
     2581 acccatgatt ttgacattgg aatttaaaag caaagcagag atgtcacatg tcccacagtc
     2641 ccaggatttc cagtttacat ggaattacag tgaccagggc ctgagtaaca ccaaagaaag
     2701 tgcttgtgtg aaagactatg atttggaaag aggccctgat gagaaaccgg atgaacctct
     2761 gcatttagac ttaagaaatg tgccccagag cctcccctgg acccggagag tctacgagtt
     2821 ctacagcgct ccgtttgtca agttttggtt ctacacgatg gcttatttgg cattcctcat
     2881 gctgttcact tacacagtgt tggtggagat gcagccccaa cccagcgtgc acgaatggct
     2941 tgtcatcatc tacatcttca ccaatgctat tgagaaggtc agggagatct gcatttcaga
     3001 accaagcaag tttaagcaga aggtgaagat gtggctcagt gagtactgga acctcatgga
     3061 aactgtggct attggcctgt ttgctgttgg ctttggccta cggtgggggc atccaccttt
     3121 gcaaactgca gggagactca tctactgcat agacatcata ttttggtttt ctcggctcat
     3181 ggacttcttt gctgtgaatc aacatgcagg tccatatgtg accatgattg ccaaaatggc
     3241 agcaaacatg ttctacattg tgatcatcat ggccattgtc ctgctgagct ttggcgtggc
     3301 gcgcaaagct atcctctcac cgaaggagcc tccttcatgg cgcctggctc gtgacatcgt
     3361 ctttgagccg tattggatga tgtatggaga agtctatgct tcagatatag atgtttgttc
     3421 aaatgagact tcttgccctc cgggttcttt cctgactcca ttcctgcaag ctgtctacct
     3481 ctttgtgcaa tacatcatca tggtgaacct tctgattgcc tgtttcaaca acatttactt
     3541 agatataaaa tccatctcga acaagctctg gaaatacaac cgctatcgct acatcatgac
     3601 ctaccaccag aagccctggc tgcctcctcc ctttatcctg ctgaatcacc tgtgtctcct
     3661 cctccgaggt ctgtgctgtc gcccagcccc acaggaccag gaagaggggg acggagggct
     3721 aaagctctat ctcactaagg atgacttgaa aaagcttcat gattttgaag agcagtgtgt
     3781 ggaaaaatat ttccacgaga agacggaagg tctgaattgt agttttgagg aacaaattcg
     3841 aatgacatca gaaagggtat cagagatgtt tttccaactg aaagaaatga atgaaaaagt
     3901 atcttttata aaggactccc tactatccct ggacagccag gtgggacacc ttcaggacct
     3961 ctcggccata actgttgaca ccttaaaggt cctttctgct gttgacacct tgcaagaaga
     4021 tgagattctt ttggccaaca gaaagcattc tacttgcaga aaacgtcccc acagctggac
     4081 caatgtcatc tgtgcaaagg ttctaagtga catggaaagc tgtgggaaga agaaacttca
     4141 gtattatagc atgccccctt ctttgctgag gagcctcgct agaagtcagc ttcccccaag
     4201 tgtgcagagg ggtgcacttg tagaggttac gcacagtaag agagaggcct cacatgtaag
     4261 agaagagcag gaggaacgag aaatggaaca gaggacaacg gcttctggga tttcccatgt
     4321 cagacaagca cactctaagt atggccagtt tctcctggtt ccatcttctg gaaagcaagt
     4381 tcctttgtct ttggaaaccc cgccacatct tttcagatca tctgaggagg caggtataga
     4441 tggtctggta ttggagcata tacaccaaag tgatctcacc acccacttgc ctcagcagac
     4501 ccctgctgcc tcccaccagg cattggtagc ggaacacaaa gaccaacatg aggcagttac
     4561 acagatgtca gacaaacctg ccaaggcaga acaagacttg ctcgcattca gtggcacacc
     4621 tgctcccatg acagtgactt ctcttccttc gagagccatc agcatgcaag atgaaggtgg
     4681 atatgtaaac tgggcatttt cagaaaatga tgaaacaggt gtgtttagct ttaaaaaaaa
     4741 gtggaaaact tgcttggcct ccacttgcaa tagtgactcc aatcctggtg gagactactt
     4801 cctgcacact ggaggtagat ccggactgga taactcaaga agactggccc agagttgtga
     4861 gtgcccagca ggaccgtgga cccaggcaag aagatccttt tggatcaatc ctctctgcag
     4921 agataaagct ctcattaaga gtcatagttt tagattccac aaggaggaga aactgaggaa
     4981 gacctggaag aacaacagtc attcaaaatc cttagagacc aggtccacat ggctcaaagc
     5041 aaaactgcta accaagaccc ggagcctgtc aaagaagaag aggaagaccc aagggctcca
     5101 ggtgccggta ataacagtca atgcctgcta tcaaagtgac cagttgaatg cagagccagg
     5161 agaaactaac accactgaag agttcagcaa gaaatggctc tctgtatcca acttcagcca
     5221 gataggttta gagccttaca tataccagaa aatgaaaatg aaggaaatca agcgacatac
     5281 tacacaagcc agtgaccacc taaggcagcc acaagagaac cgagataaaa cccccacatg
     5341 gaattctggg agcaccagcc tcagcaggag ttttctaaca agaagtccaa atgaagttca
     5401 caagatctca acctccttaa aaagccctca agagcctcac caccattatt cagccattga
     5461 aaggaataat ttaatgagac tgtctcagac cataccgttt acaccaatcc agctgttcac
     5521 aggagaggaa gtgaccatct acaagcttga agaaagttct cctctgaccc tggataagag
     5581 catgtcctct tggtcgcagc atggcagagc tgccatgatt caggtgctgt cacaagagga
     5641 aatggatggg ggtctccgca aagccatgcg agttatcagc acctggtctg aggatgatgt
     5701 tctcaagcct gggcaggttt tcattgtgaa gtcttttctc cccgaggttg tgcagacgtg
     5761 gtataaaatc ttccaggaga gcactgtgct tcatctttgc cttagggaaa ttcaacagca
     5821 aagagctgcc caaaaactca tctatacctt taaccaagta aaaccacaaa ccattcccta
     5881 tacaccaagg tttctcgagg tttccttggt ctactgccat tcagccaacc aatggttaac
     5941 cattgagaag tacatgacag gggagttccg gaaatacaat aacaacaatg gtgatgaaat
     6001 agctcccacc aataccctgg aagaactgat gttggctttc tctcactgga cctatgaata
     6061 tacccgggga gagctgctgg ttttagattt gcaaggtgtt ggagaaaatt tgacagatcc
     6121 gtctgttata aaaccggaag acaaacaatc aagagggatg gtgtttggac cggccaattt
     6181 aggggaagat gcaattagaa gcttcattgc aaaacatcgc tgcaactcct gctgtgggaa
     6241 gctcagactg ccggatttaa aaaggaatga ttactccctt tcaagaacac actgcaactt
     6301 gggatttggg caaaccattg aaccaactga ggagcttcca gaaagagaca aaaatagaag
     6361 ttccctggaa gatcacacac gcctttaaaa agatgatgaa ggaagacggt ggtcctttag
     6421 ctccttctgc catgacttct atagtgatgg acatagactg gcatgatcct gactatgtca
     6481 gaatctccat caccatgact ttacaatgtg gacctccctg gaagtgccct ctgagcctca
     6541 tctcccactg cctagagggc acagtgcata atggaggggt tctcctgggt attgacttct
     6601 gagaagaatg tgtggcatgc gttctcatct cccggttctg catgaagatg ctagatgagt
     6661 cagttgggaa tactttcctc tatacctcat tgcttcagct ggccacttgg agtagaatgc
     6721 tgtgcactaa cgaactaggg aataaattaa tttattttct ca
//
