LOCUS       NM_001242358            2327 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tRNA nucleotidyl transferase, CCA-adding, 1 (Trnt1),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001242358
VERSION     NM_001242358.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Nagaike,T., Suzuki,T., Tomari,Y., Takemoto-Hori,C., Negayama,F.,
            Watanabe,K. and Ueda,T.
  TITLE     Identification and characterization of mammalian mitochondrial tRNA
            nucleotidyltransferases
  JOURNAL   J Biol Chem 276 (43), 40041-40049 (2001)
   PUBMED   11504732
REFERENCE   3  (bases 1 to 2327)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK135791.1, AK152739.1, AK139764.1 and BU755617.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152739.1, SRR1660811.172150.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK135791.1         21-50
            31-1299             AK152739.1         2-1270
            1300-2283           AK139764.1         1217-2200
            2284-2327           BU755617.1         1-44                c
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 49.24 cM"
     gene            1..2327
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /note="tRNA nucleotidyl transferase, CCA-adding, 1"
                     /db_xref="GeneID:70047"
                     /db_xref="MGI:MGI:1917297"
     exon            1..119
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            120..293
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1450
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /EC_number="2.7.7.72"
                     /note="isoform a is encoded by transcript variant 1; tRNA
                     adenylyltransferase, mitochondrial;
                     tRNA-nucleotidyltransferase 1, mitochondrial; CCA tRNA
                     nucleotidyltransferase 1, mitochondrial; mt CCA-adding
                     enzyme; mt tRNA CCA-diphosphorylase; mt tRNA
                     adenylyltransferase; mt tRNA CCA-pyrophosphorylase;
                     mitochondrial tRNA nucleotidyl transferase, CCA-adding"
                     /codon_start=1
                     /product="CCA tRNA nucleotidyltransferase 1, mitochondrial
                     isoform a"
                     /protein_id="NP_001229287.1"
                     /db_xref="CCDS:CCDS20397.1"
                     /db_xref="GeneID:70047"
                     /db_xref="MGI:MGI:1917297"
                     /translation="MQSVLYPWHRQVLRCSWSRLCLLKRYLFTMKLQSPEFQSLFTEG
                     LKSLTELFAKENHELRIAGGAVRDLLNGVKPQDVDFATTATPTQMKEMFQSAGIRMIN
                     NKGEKHGTITARLHEENFEVTTLRIDVTTDGRHAEVEFTTDWQKDAERRDLTINSMFL
                     GFDGTLFDYFNGYADLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVDRPGDHDHE
                     TLEAIAENAKGLAGISGERIWVELKKILTGDHVNHLIHLIYDLGVAPHIGLPANANLE
                     EFNKVSKNVEGFSPKPMTLLASLFKVQDDVTKLDLRLKISKEEKNLGLFIVKNRKDLI
                     KATDSSEPLKPYQDFVIDSREPDATARVCELLKYQGEHGLLKEMQQWSVPPFPVSGHD
                     IRKVGISSGKEIGALLQQLREQWKKSGYRMEKDELLSYIKKT"
     exon            294..487
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            488..626
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            627..753
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            754..947
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1201
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1483
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..2311
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2286..2291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /note="hexamer: AATAAA"
     polyA_site      2304
                     /gene="Trnt1"
                     /gene_synonym="2410043H24Rik; 2610044E04Rik;
                     9830143O18Rik; CGI-47; mt-Trat"
                     /note="major polyA site"
ORIGIN      
        1 tgacgtcact ggtccgcgac ccggaagtgc gcgtgcggcc cgggcagcag gcgggtaagc
       61 gggtcttcgg agtaggagcg ccacgtttct aagacacctt ggcgacctgg ctgtgcagca
      121 tgtgtgattg gcgacttgtc tccagatgca gagcgtcctg tatccttggc acaggcaggt
      181 cctgaggtgt agttggagta gattgtgcct tctgaagcga tacctgttta caatgaagtt
      241 gcagtcccca gaattccagt cccttttcac cgaaggattg aagagcctga cagaattatt
      301 tgccaaagag aatcatgaat taagaatagc aggaggagca gtgagggatt tactgaatgg
      361 ggtgaagcct caagatgtgg atttcgccac cactgccacc cctactcaga tgaaggagat
      421 gttccagtca gctggcattc gcatgatcaa caacaaagga gagaaacatg ggactatcac
      481 tgccaggctt catgaagaaa attttgaagt tactacactc cgaattgatg ttaccactga
      541 tggaagacat gcagaggtag aattcacaac tgactggcag aaagacgctg aacgaagaga
      601 tctcactata aattctatgt ttctaggttt tgatggtaca ctgtttgatt acttcaatgg
      661 ttatgcagat ttaaaaaata aaaaggttcg atttgttgga catgcaaaac agagaattca
      721 ggaagattat cttcgaattt taaggtattt caggttttat ggcagaattg tcgacagacc
      781 tggtgaccat gaccatgaga ctctagaagc aattgcagaa aatgccaaag gtttggctgg
      841 aatatcagga gagaggattt gggtagaact gaagaaaatt cttactggtg accatgtaaa
      901 ccatttgatc cacctgatct atgatcttgg tgtggctcct cacataggtt tacctgctaa
      961 tgcaaattta gaagaattta acaaagtcag taaaaatgtt gaaggctttt caccaaaacc
     1021 aatgactctt ttggcttctt tattcaaagt acaagatgat gtcacaaaat tggacttgag
     1081 attgaaaatt tcaaaagagg agaaaaacct gggtttattt atagttaaaa acaggaaaga
     1141 cttgattaaa gcaacagata gttcagaacc attgaaacca taccaagact ttgtgataga
     1201 ctctagggaa ccagatgcaa ctgcccgtgt ttgtgagctg ctgaagtacc aaggagagca
     1261 tggtcttctc aaagagatgc agcagtggtc tgtcccgccg ttccctgtga gtggacatga
     1321 catcaggaaa gtgggtattt cttcagggaa ggaaattggg gccctgctac agcagttgcg
     1381 tgaacagtgg aagaaaagtg gttaccggat ggaaaaagat gaactactaa gttacataaa
     1441 gaagacctaa agatgagccc tgtatgaaac aggggaagtc ttgaaagttc atctaaaact
     1501 aaatgaagag tcaacaagaa aagtaaacag ctgtattacc cgggaaacca gctgtgcact
     1561 gtagaaggcc ggcctatcag attcaggttg gacgctttat acacagtcag tgtctcatgt
     1621 acatatccat gaggattcac atatgctgcc attggaccac ataaggataa actacagaca
     1681 aagaaataat gtggagaata agacaatgaa aagaaaaata gtaatcccag ttttttactt
     1741 atttgggaca taagttatgc tttttaacct ttgaatttta gatattatat agtactttgt
     1801 gtaaaatgta agacaagtat acaggaggtt ggcagatatt ctgttgctta aaaatgttct
     1861 ttgatgattc aaaggtaaat atatgtgttt acaaagccta gtgaggtcat gttttcatct
     1921 ttggacattt agttacttta cctacatatc agtttcctga ccttcttgat ctgtagttct
     1981 aatgaattca tcaaagtgtt aagcttcaca gcatcagcac atgttcagag acccacccaa
     2041 ttttggtccc gtgtagcaga actagctgaa gtataatgct tgaagcattc ctattatgct
     2101 ttaagatagg atgtgtctaa acaaaaattg aggggaaaaa gactgtatta cagatgtttc
     2161 caggctgtaa gacagcttgc cctgtgttta tctcctttac tgagataaac gcatattttt
     2221 taggcactgg agaaaatatg gaaagtggaa ccacagaagg gaatatggaa atatattaaa
     2281 aatgtaataa agatcttacc taaatatttc aaaaaaaaaa aaaaaaa
//
