LOCUS       NM_028063               1520 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Mus musculus tRNA 5-methylaminomethyl-2-thiouridylate
            methyltransferase (Trmu), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_028063
VERSION     NM_028063.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   He,Q., Zhao,Q., Li,Q., Pan,R., Li,X. and Chen,Y.
  TITLE     Mtu1 defects are correlated with reduced osteogenic differentiation
  JOURNAL   Cell Death Dis 12 (1), 61 (2021)
   PUBMED   33431792
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wu,Y., Wei,F.Y., Kawarada,L., Suzuki,T., Araki,K., Komohara,Y.,
            Fujimura,A., Kaitsuka,T., Takeya,M., Oike,Y., Suzuki,T. and
            Tomizawa,K.
  TITLE     Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is
            Required for Mitochondrial Translation and Is Involved in
            Reversible Infantile Liver Injury
  JOURNAL   PLoS Genet 12 (9), e1006355 (2016)
   PUBMED   27689697
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1520)
  AUTHORS   Yan,Q. and Guan,M.X.
  TITLE     Identification and characterization of mouse TRMU gene encoding the
            mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase
  JOURNAL   Biochim Biophys Acta 1676 (2), 119-126 (2004)
   PUBMED   14746906
  REMARK    GeneRIF: plays a role in mitochondrial tRNA modification pathway
            and the pathogenesis of mitochondrial disorders
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139513.10.
            
            On Nov 17, 2006 this sequence version replaced NM_028063.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005541.1, BC061026.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-132               AC139513.10        98459-98590
            133-298             AC139513.10        101806-101971
            299-405             AC139513.10        104218-104324
            406-528             AC139513.10        109337-109459
            529-701             AC139513.10        111699-111871
            702-755             AC139513.10        112958-113011
            756-822             AC139513.10        113086-113152
            823-923             AC139513.10        114058-114158
            924-1068            AC139513.10        115492-115636
            1069-1151           AC139513.10        115967-116049
            1152-1520           AC139513.10        116157-116525
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 40.42 cM"
     gene            1..1520
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /note="tRNA 5-methylaminomethyl-2-thiouridylate
                     methyltransferase"
                     /db_xref="GeneID:72026"
                     /db_xref="MGI:MGI:1919276"
     exon            1..132
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1304
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /EC_number="2.8.1.14"
                     /note="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase 1"
                     /codon_start=1
                     /product="mitochondrial tRNA-specific 2-thiouridylase 1"
                     /protein_id="NP_082339.1"
                     /db_xref="CCDS:CCDS27726.1"
                     /db_xref="GeneID:72026"
                     /db_xref="MGI:MGI:1919276"
                     /translation="MSALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDE
                     QGVCAADKDCEDAYKVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDINCN
                     KHIKFSCFYHYAVDNLGADAVATGHYARTSLEDEEVFEQKHTKKPDGLFRNRFEVRNP
                     VKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGELTKDFVKKIAAENSLHHVLQKRES
                     MGICFIGKRNLEHFLLQYLQPRPGKFVSIEDNTVLGTHKGWFLYTLGQRAKISGLREP
                     WYVVEKDGTKGDVLVAPRVDHPALYRDLLRTNRVHWIAEEPPAALVRDKMMECHFRFR
                     HQMALVPCVLTLNQDGTVWVTAVKAVRGLALGQFAVFYKGEECLGSGKILRLGPSAYT
                     LQKGKNRTRVAPEASSDSPGLHPTS"
     exon            133..298
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            299..405
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            406..528
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            529..701
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            702..755
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            756..822
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            823..923
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1068
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1151
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1520
                     /gene="Trmu"
                     /gene_synonym="1110005N20Rik; 1600025P05Rik; Mtu1; Trmt1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggacttccgg cgtagcttgg acgcagccag aaagctcgcg gtgtagcctg atgtcggcgc
       61 tgcggcacgt cgtgtgcgcc ctgtctggcg gtgtggacag cgcggtggcg gcgctgctgt
      121 tgcggcggag aggttaccag gtgacagggg tatttatgaa gaactgggac tcgctggatg
      181 agcagggcgt ttgtgctgct gacaaggact gtgaagatgc gtataaagtt tgtcagatct
      241 tagacatccc tttccaccag gtgtcctatg tgaaggagta ttggaatgat gtgttcagtg
      301 actttttgaa cgagtatgag aaaggaagga ctccaaaccc cgacatcaac tgcaataagc
      361 atatcaagtt cagttgcttt tatcactatg ctgtggacaa tcttggagca gatgccgttg
      421 ccactggcca ctacgccagg acctcactgg aggatgagga agtctttgag caaaagcaca
      481 ctaagaagcc tgacgggctg ttcagaaatc gatttgaagt cagaaacccc gtaaaacttc
      541 tccaagcagc tgacagcttt aaagaccaga ccttctttct cagccaggtt tcccaggatg
      601 ccctgagaag gaccatcttt cccctggggg aactaacaaa agattttgta aagaaaatag
      661 ccgcggagaa tagcctccat cacgtgcttc agaagagaga gagcatgggc atctgcttca
      721 ttggtaagag aaacttggag catttccttc ttcagtattt gcagcctcgg cctggaaagt
      781 ttgtttctat cgaggataat acagttctgg gaacacacaa aggttggttc ctgtacactt
      841 tgggccagag agctaagatc agcggcttga gagagccctg gtacgtggtg gagaaggatg
      901 gcaccaaggg tgacgtgctt gtggctcccc gggtggacca cccagctctg tacagggacc
      961 tgctcaggac caaccgagta cactggattg cggaggagcc acctgctgcc ttggtcagag
     1021 acaagatgat ggagtgccat ttccgattcc gccaccagat ggcactagtg ccctgcgtgc
     1081 tgactctcaa tcaggatggc actgtgtggg taacggctgt gaaggctgtg aggggccttg
     1141 ccctgggaca gtttgcagtg ttctacaagg gtgaggagtg cctcggcagt gggaaaatcc
     1201 tgcggctagg gccgtctgcc tacacactcc agaagggtaa gaacagaact cgagtggctc
     1261 ccgaggcctc cagtgacagc ccaggcctgc accccacatc ctgacaaagg ccaaggagga
     1321 ggcattagag cagagtcgtc agtgatgggt gttagtgctg ccagaatcaa taggcgggct
     1381 ggctgcagag gaaggagggt ctacgctgct gctccaggaa accagagcgt gtgctggctg
     1441 tgggccctga tgcagctgct cttccacagc atccaggagg ccaatggaga aaccgcatca
     1501 aataaaactg tcaaagtctg
//
