LOCUS       NM_001099793            2000 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tRNA methyltransferase 61A (Trmt61a), transcript
            variant 3, mRNA.
ACCESSION   NM_001099793
VERSION     NM_001099793.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Liu,Y., Zhou,J., Li,X., Zhang,X., Shi,J., Wang,X., Li,H., Miao,S.,
            Chen,H., He,X., Dong,L., Lee,G.R., Zheng,J., Liu,R.J., Su,B.,
            Ye,Y., Flavell,R.A., Yi,C., Wu,Y. and Li,H.B.
  TITLE     tRNA-m1A modification promotes T cell expansion via efficient MYC
            protein synthesis
  JOURNAL   Nat Immunol 23 (10), 1433-1444 (2022)
   PUBMED   36138184
  REMARK    GeneRIF: tRNA-m[1]A modification promotes T cell expansion via
            efficient MYC protein synthesis.
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Ozanick,S., Krecic,A., Andersland,J. and Anderson,J.T.
  TITLE     The bipartite structure of the tRNA m1A58 methyltransferase from S.
            cerevisiae is conserved in humans
  JOURNAL   RNA 11 (8), 1281-1290 (2005)
   PUBMED   16043508
  REMARK    GeneRIF: Shows conservation of the yeast TRM61 (GCD14) protein in
            mouse, human and Drosophila, and demonstrates functional activity
            of the human ortholog.
REFERENCE   3  (bases 1 to 2000)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB868135.1, BC051186.1 and AI447734.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC051186.1, AK032824.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849374, SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-332               BB868135.1         2-333
            333-1791            BC051186.1         728-2186
            1792-2000           AI447734.1         1-209               c
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 61.09 cM"
     gene            1..2000
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /note="tRNA methyltransferase 61A"
                     /db_xref="GeneID:328162"
                     /db_xref="MGI:MGI:2443487"
     exon            1..57
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /inference="alignment:Splign:2.1.0"
     exon            58..417
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..959
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /EC_number="2.1.1.220"
                     /note="tRNA (adenine-N(1)-)-methyltransferase catalytic
                     subunit TRM61; tRNA(m1A58)-methyltransferase subunit
                     TRM61; tRNA(m1A58)MTase subunit TRM61; tRNA
                     (adenine-N(1)-)-methyltransferase catalytic subunit
                     TRMT61A; tRNA(m1A58)MTase subunit TRMT61A;
                     tRNA(m1A58)-methyltransferase subunit TRMT61A; tRNA
                     methyltransferase 61 homolog A; mRNA
                     methyladenosine-N(1)-methyltransferase catalytic subunit
                     TRMT61A"
                     /codon_start=1
                     /product="tRNA (adenine(58)-N(1))-methyltransferase
                     catalytic subunit TRMT61A"
                     /protein_id="NP_001093263.1"
                     /db_xref="CCDS:CCDS26184.1"
                     /db_xref="GeneID:328162"
                     /db_xref="MGI:MGI:2443487"
                     /translation="MSFVAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLR
                     HSVDLIGRPFGSKVICSRGGWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELR
                     PGSVVCESGTGSGSVSHAIIRSVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVH
                     TQDVCCSGFGVVHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQ
                     ALAAHGFTELSTLEVLPQVYNVRTVSLPLPDLGANNLETNMGSDASPFRSGTPMKETV
                     GHTGYLTFATKTPG"
     exon            418..684
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /inference="alignment:Splign:2.1.0"
     exon            685..1992
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1971..1976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /note="hexamer: AATAAA"
     polyA_site      1992
                     /gene="Trmt61a"
                     /gene_synonym="6720458F09Rik; Gcd14; Trm61"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgcta cggccgccgt ggagccacgt gtggagccgg gagccggttt atgctgcgtc
       61 cctgaccctt ggcaaacgtt aacaccatga gtttcgtggc atacgaggag ctgatcaagg
      121 aaggcgacac agccatcctg tcactggggc acggctcgat ggtagcagtg cgtgtgcagc
      181 gtggggcaca gacccagacc cggcatggcg tcctgaggca ctcggtggac ctcattggcc
      241 gcccatttgg ctccaaggtg atttgcagca gaggcggctg ggtgtatgtg ctgcacccca
      301 ctcctgagct ctggacagtg aacctgcccc accgcacgca gatcctctac tccaccgaca
      361 ttgccttgat caccatgatg ctggagctgc gtcccggttc tgtggtctgt gaatcaggca
      421 cgggcagtgg ttctgtatcc cacgcaatca tccgcagcgt tgcccccact ggccacctac
      481 acacagtaga gttccaccag caacgggcgg acaaggcccg ggaggaattc caggagcatc
      541 ggctgagcca gtgggtgact gtgcacaccc aggatgtgtg ctgcagtggc ttcggtgtgg
      601 tccacgtggc tgacgctgtc ttcttggata tcccatcacc ctgggaagct gtgggccatg
      661 cctgggatgc cctcaaggtt gaaggtgggc gtttctgctc cttctctccg tgcattgagc
      721 aggtgcagcg gacatgccag gcgctggcag cccatggctt cacagagctt agcaccctgg
      781 aggtactgcc acaggtctac aatgtgcgca ctgtcagtct gcccttgccc gacctggggg
      841 ccaacaactt agagaccaac atgggctctg atgccagccc cttccgtagt ggcacaccca
      901 tgaaggagac tgtgggccac actggctacc tgacttttgc cactaaaacc ccaggctagc
      961 aggcctccaa atgaacagca caggcaagca ggcagggagg ccagaggctg ccttatatgg
     1021 tcagccactg ccctcgtggc ttgtgtttag aaagagatgg gggcggggtg gggcaggggc
     1081 ctcctgggtg gctgaggggg gtgagtaggc tggccctgct cagctgggag gcattcctgt
     1141 cttctgagag ggaagtctcc acttcatcac tgctcagcgc aagctctgtc ctggctcttt
     1201 gttgtcaaca tgagtctttc ctctccatag tccttcctgg gttttgagac taaatggtaa
     1261 aagttgaggg cgtcggaagc tttgggcact gcctatagcc tgggctgacc tacgggagcg
     1321 tgggttgtcc catccctggc gcaagtctcc aggccctggt gctccagagc ttagccagaa
     1381 gctgtggttg agctaaatta accagaaagc ccagccctgc cctcctctcc ctatacttcc
     1441 ctaaggctga tggagggacc aggctggccc aggaacaaag gaggtgatga taatcaataa
     1501 tcatgtgctg cacaggccac gtgtgtgtca gcacgaccgt gtgtgtgtgt gtgtgtgtgt
     1561 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg agttcctggt
     1621 gtgctggtgt gctgtcatct ggggttaggc atcatcctga gggatagaac acagcaggat
     1681 tggtccctgc ttccgtgacc ttccagtcct tgttggagct ggggtcaaag cggatcatag
     1741 aggagcctgt gcatgggcta agagcctggc tgtggacttg ccacatgtgt caccttggcc
     1801 tggtgtccat tctcggtatg tggctagctt caagggagtt tcagtgctcg gtgctcagat
     1861 catcttggtg tgaggctgcc agtcagctgc tgctgctgcg tggaagcagc catcgcgaga
     1921 ctcaccagtg tcctactgtc atatgtcata cccgagggca cccaaaggat aataaatatt
     1981 ttccccactg tgaaaaaaaa
//
