LOCUS       NM_029580               2096 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus TRM5 tRNA methyltransferase 5 (Trmt5), transcript
            variant 1, mRNA.
ACCESSION   NM_029580
VERSION     NM_029580.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Perniss,A., Liu,S., Boonen,B., Keshavarz,M., Ruppert,A.L., Timm,T.,
            Pfeil,U., Soultanova,A., Kusumakshi,S., Delventhal,L., Aydin,O.,
            Pyrski,M., Deckmann,K., Hain,T., Schmidt,N., Ewers,C., Gunther,A.,
            Lochnit,G., Chubanov,V., Gudermann,T., Oberwinkler,J., Klein,J.,
            Mikoshiba,K., Leinders-Zufall,T., Offermanns,S., Schutz,B.,
            Boehm,U., Zufall,F., Bufe,B. and Kummer,W.
  TITLE     Chemosensory Cell-Derived Acetylcholine Drives Tracheal Mucociliary
            Clearance in Response to Virulence-Associated Formyl Peptides
  JOURNAL   Immunity 52 (4), 683-699 (2020)
   PUBMED   32294408
  REMARK    GeneRIF: Chemosensory Cell-Derived Acetylcholine Drives Tracheal
            Mucociliary Clearance in Response to Virulence-Associated Formyl
            Peptides.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159813.2 and AC122025.3.
            
            On Mar 15, 2023 this sequence version replaced NM_029580.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012521.1, AK049127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AC159813.2         192655-192713
            60-700              AC122025.3         201572-202212       c
            701-825             AC122025.3         199549-199673       c
            826-1477            AC122025.3         197942-198593       c
            1478-2096           AC122025.3         196951-197569       c
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 30.42 cM"
     gene            1..2096
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /note="TRM5 tRNA methyltransferase 5"
                     /db_xref="GeneID:76357"
                     /db_xref="MGI:MGI:1923607"
     exon            1..59
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1560
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /EC_number="2.1.1.228"
                     /note="isoform 1 is encoded by transcript variant 1; tRNA
                     (guanine-N(1)-)-methyltransferase; TRM5 tRNA
                     methyltransferase 5 homolog; M1G-methyltransferase; tRNA
                     [GM37] methyltransferase; tRNA
                     (guanine(37)-N1)-methyltransferase; tRNA
                     (guanine(37)-N(1))-methyltransferase"
                     /codon_start=1
                     /product="tRNA (guanine(37)-N(1))-methyltransferase
                     isoform 1"
                     /protein_id="NP_083856.1"
                     /db_xref="CCDS:CCDS36474.1"
                     /db_xref="GeneID:76357"
                     /db_xref="MGI:MGI:1923607"
                     /translation="MRIVWKLFGFSRRLLQVEWCHPSESILLFTLVPRLRKAPSVFLL
                     GQRQGLSTMPEIEASVRDSELFSPPSDVRGMRELDRTAFKKTVSIPVLKARKEVVNRL
                     MRALRRVALQRPGIKRVIEDPKDEDSRLIMLDPYRMLTADSFDKAELGVLKELDVSPQ
                     LSQYNLELTYENFKSEEILKAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFKHLIGQV
                     MVDKNPGITSAVNKTSNIDNTYRNFQMEVLCGEENMLTKVRENNYTYEFDFSKVYWNP
                     RLSTEHGRITELLNPGDVLFDVFAGVGPFAIPAARKNCTVFANDLNPESHKWLLHNCK
                     LNKVDQKVKVFNMDGKDFIQGPVREELMLRLGLSAEAKPSVHIVMNLPAKAIEFLSVF
                     RSLLDGQPCSTELLPTVHCYCFSKDSDPAKDVRQQAEAVLGVSLETSSSVHLVRNVAP
                     NKEMLCITFQIPTATLYRNQSLSLQNDQEPPLKRQKTGDPFSGEPQIASDS"
     exon            60..700
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /inference="alignment:Splign:2.1.0"
     exon            701..825
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1477
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..2096
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1692..1697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /note="hexamer: AGTAAA"
     polyA_site      1712
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /note="major polyA site"
     regulatory      2074..2079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
                     /note="hexamer: AATAAA"
     polyA_site      2096
                     /gene="Trmt5"
                     /gene_synonym="2610027O18Rik; mKIAA1393"
ORIGIN      
        1 agcagtgatg acgtcaccag cctccgacgc cctatttccg agctgcacca gagcatgaga
       61 atcgtatgga agctatttgg attctcaaga agacttttgc aagtggaatg gtgtcacccc
      121 agtgaatcaa tattgttgtt taccctggtg ccgagactta ggaaagcgcc ttctgttttc
      181 ttgttgggtc aaagacaagg actctcaacc atgccagaaa tagaagcaag tgtgagagat
      241 agtgaattat tttcaccacc ctctgacgtt cgaggaatga gagaacttga tagaacagct
      301 tttaagaaga cagtgtccat ccctgtgctt aaagcgagga aagaagtagt caacagattg
      361 atgcgagccc tcaggagagt agccctgcag cgcccgggca taaagcgagt gattgaggat
      421 cccaaggatg aagacagtag gctgatcatg ttggacccct acaggatgct tactgctgat
      481 tcctttgaca aagcagaact cggagtttta aaagaacttg atgtcagccc acagttatcc
      541 cagtacaact tggaactaac atatgaaaat tttaagtcag aagaaatttt gaaagctgtg
      601 cttcctgaag gtcaagatgt aacctctgga ttcagcaggg ttgggcatat tgcacacctg
      661 aatctccgag atcaccagct gcccttcaag catttaattg gccaagttat ggttgacaaa
      721 aacccaggaa tcacctcagc agtaaataaa accagcaaca ttgacaatac gtaccgaaat
      781 ttccaaatgg aagtgctgtg tggagaggaa aatatgctga ccaaggttcg agaaaacaac
      841 tacacgtatg agtttgattt ctctaaagtc tactggaatc ctcgtctctc tactgaacat
      901 ggccggatca ctgaacttct caaccctgga gatgtcttgt ttgatgtttt tgctggggtt
      961 gggccctttg ccattccagc agcaaggaaa aactgcacag tgtttgccaa tgatctcaat
     1021 cctgagtccc ataaatggct gttgcacaat tgtaaactga ataaagttga ccagaaagtg
     1081 aaagtcttta acatggatgg aaaagacttc atccaagggc cagtcagaga agagttaatg
     1141 cttcggctag gattgtcagc tgaagctaaa ccctctgtcc acattgtcat gaacttgcca
     1201 gctaaggcca tagagtttct cagtgttttc agatcacttt tggatgggca gccttgcagc
     1261 acggagctcc tccccacagt gcactgttac tgcttctcca aagatagcga tcctgctaag
     1321 gatgttcgtc agcaggcgga agctgtgctg ggcgtttcct tggagaccag tagttcagtt
     1381 catctcgtaa ggaatgtggc ccctaacaag gagatgctgt gcatcacctt ccagattccc
     1441 actgctacac tctacaggaa ccaaagcctc agtctacaga atgaccaaga accacctctt
     1501 aaacggcaga agacaggtga cccgttctca ggagaaccac agatcgcatc agattcttag
     1561 tttgaaatgt tttctccatc tccccagtag actttatgta aagaagtaca atttgtaaga
     1621 ggtaaactct accacacaga tatttgttac taaacactta cgatttgctc ttataaatgg
     1681 aacattacca aagtaaactg aaataagagg aacttggata ttgtatagac tgattttttg
     1741 tttccatcca tttttccttt cttttttatt attttttgtg gcaggatctg tgtaactcag
     1801 ggcttagaac tctatcttcc tgcttcagcc tcccagctgt tggcatttcc agcgtgcacc
     1861 gctgttattc agcatagttt taaaacagaa tcggcatggg ccagtcagac cagtcagatg
     1921 gctcactggg tgaaggcact tgctgccaag cctgatagcc tgagcctgcc taggaacaac
     1981 gtggaaagga agaaatcctg aaagtcttct tctgacctca tgcgtgcacc aaggtactca
     2041 tgtatacact tgcacaaaat aagcgaacaa attaataaac ttggaaattt atgtaa
//
