LOCUS       NM_030208               3784 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tRNA methyltransferase 44 (Trmt44), mRNA.
ACCESSION   NM_030208
VERSION     NM_030208.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132402.1 and AC116705.11.
            
            On Jul 23, 2009 this sequence version replaced NM_030208.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132402.1, AK046957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2677              AK132402.1         2-2678
            2678-3784           AC116705.11        194880-195986       c
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 18.25 cM"
     gene            1..3784
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /note="tRNA methyltransferase 44"
                     /db_xref="GeneID:78890"
                     /db_xref="MGI:MGI:1926140"
     exon            1..512
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..2167
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /EC_number="2.1.1.211"
                     /note="methyltransferase-like protein 19"
                     /codon_start=1
                     /product="probable tRNA (uracil-O(2)-)-methyltransferase"
                     /protein_id="NP_084484.2"
                     /db_xref="CCDS:CCDS19230.1"
                     /db_xref="GeneID:78890"
                     /db_xref="MGI:MGI:1926140"
                     /translation="MEELGRVRLSDPDALLPAGFWAAVTVWLERPQVANKRLCGARME
                     ARGRTLRSHAQAECGPRQGQGHGLEREPGQASPKGEPESGPRASREGTAPAADLNSLW
                     DRVSQSLVHANPEMLAFLCGPSLGPQPEAAQELDLILRTVIPKASPHSPLTEPKKELV
                     VQDVSSGSVTFLPLEEDNEGNLEVKTSNVYQLHLHHNEGEWFISVLIFCPERWHSDGV
                     VYPKPAWLGEELLSKLARWAVENRKSEFKSTLSLVSILRYSRMYQELKEKYRDMVKVW
                     PEVTDPEKFVYEDVAIATYLLILWEEERAEKGVTTKQSFVDLGCGNGLLVHILSNEGH
                     PGRGIDIRRRKIWDMYGPQTQLEEGSITPSDETLFPGVDWLIGNHSDELTPWIPVIAA
                     RSSYTCRFFVLPCCFFDFVGRYQRQQSRKTQYREYLDFVLEVGLSCGFHVQEDCLRIP
                     STKRVCLIGKSRTYPPSAEVWMDEQRTRYLHSRQGHPQSRPGGAHAPSAPQTAAHDAG
                     LQDSCRTVNAGSECVLEGLAAERGAGAPAPGLWVPGFCPREKAERVRNCAALPRDFVD
                     QVVLQVANLLLDRKKFNTGNSEARSLKPWNGGGSLSLAEVAAELNSETLQRLKRECGG
                     LQTLLKNSHQVFEVLNGRVHIRDWRQELQRGKPPEAKQNLSAAVFKTRICWFFAHHPD
                     GCVLPAAQCPFAHGPEELRLSQTLKKQRQAP"
     exon            513..627
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            628..847
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            848..916
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1024
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1096
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1203
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1387
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1820
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1937
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..3784
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2680
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
     regulatory      2765..2770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /note="hexamer: AATAAA"
     polyA_site      2787
                     /gene="Trmt44"
                     /gene_synonym="2310079F23Rik; Mettl19"
                     /note="major polyA site"
ORIGIN      
        1 atcccaacgc gccgagcgcg cagccatgga ggagctgggt agagtgcggc taagcgatcc
       61 tgatgccctg ctacctgcag gcttctgggc cgccgtgacc gtgtggctgg agaggccaca
      121 ggtggccaac aagaggctgt gcggcgcccg catggaagcc cgcgggagaa ctttgcggtc
      181 ccacgcccag gcggagtgtg gaccgcggca gggacaggga cacgggctgg agagggaacc
      241 cggacaggct tctcctaaag gagagccaga gtcgggacct agggcgagcc gcgaaggcac
      301 cgctcccgcg gctgacctga actcgctatg ggaccgagtc tcccaaagcc tcgtccacgc
      361 caatccggaa atgctggcct tcctctgtgg cccctcgttg ggtcctcagc ctgaagccgc
      421 gcaggagctc gacctgattc tcaggactgt catcccgaaa gcgagcccgc actccccact
      481 cacagagccg aagaaagaac tggtagtgca agatgtctcc agtgggagtg tcaccttttt
      541 gcccttggaa gaagataatg aggggaattt ggaggttaaa acgagcaacg tgtatcaact
      601 ccatctccat cacaacgaag gagaatggtt catatctgtt ttaattttct gtccagagag
      661 atggcattca gatggtgttg tctatcctaa gcctgcctgg ctcggagaag aactgctgtc
      721 caagctggcc aggtgggcgg tggagaacag gaagagcgag tttaaaagca cactttccct
      781 ggtgtctatc ctgcgctata gcaggatgta ccaggagctc aaagagaaat acagagacat
      841 ggtaaaggtt tggcctgaag taacagatcc tgaaaagttt gtctatgaag atgtggccat
      901 tgctacgtat ctgctgatcc tatgggaaga ggaaagggct gagaagggag tcaccaccaa
      961 gcagtctttc gtggacttgg ggtgtggaaa tggccttctg gtccacatct tgagcaacga
     1021 ggggcaccca ggtagaggga ttgatatccg aagaagaaaa atctgggaca tgtatggacc
     1081 ccaaactcag ctggaggaag gctccatcac tccgagtgac gagaccctgt tccctggtgt
     1141 tgattggctg attggtaacc attctgatga gctcaccccc tggatccctg tcattgcagc
     1201 caggtcttcc tacacctgtc gtttctttgt cctgccctgc tgttttttcg actttgtggg
     1261 gcgataccag cggcagcaga gcaggaagac acagtaccga gagtatctgg actttgtgct
     1321 ggaggtgggg ctctcctgtg gcttccacgt gcaggaggac tgcctcagga ttccttctac
     1381 taagcgggtc tgcctcattg ggaaatccag aacatacccg ccttctgcag aggtctggat
     1441 ggatgagcag agaacgcgat acctgcacag cagacagggc catccacaga gccggcctgg
     1501 aggggcacat gcaccttctg caccgcagac tgctgctcat gacgctggtc tccaggacag
     1561 ttgcaggacc gtcaatgctg ggtctgagtg cgtgctggag ggcctggctg ctgagcgagg
     1621 agctggagcc ccagccccag gactctgggt gcctgggttc tgccctagag aaaaggccga
     1681 gcgtgtgagg aattgtgcag ccctccctcg agacttcgtt gaccaagtgg ttttgcaggt
     1741 ggcaaacttg ttgttagata gaaagaagtt caacacagga aactctgaag ccaggagtct
     1801 gaagccctgg aatggaggag ggagcctttc cttggcagaa gtggctgcag aactaaactc
     1861 agagacgctg cagcggctga agcgggagtg tggaggccta cagacccttc tcaagaatag
     1921 ccatcaagtg tttgaagttc tgaatgggcg cgttcacatc cgtgactggc ggcaggagct
     1981 gcagagggga aagcctccag aagccaagca gaacctgtct gcagcagtgt tcaagactcg
     2041 gatctgctgg ttcttcgccc accatcctga tggctgcgta ctgcctgctg cccaatgccc
     2101 ctttgcccat gggcccgaag agctgcgcct gtcacagacc ttgaagaagc agaggcaggc
     2161 tccctgagtc acctcttcag tgttctctag ctgttggttt cccttgtcct gatgacttca
     2221 cgctggggct gggttggctt cagtatgagc tgttggtgtg cacagccact gtcagtcact
     2281 gggacgtgtc tgcaggcagt ggagcctgct cagcccttta tgactgctct ggtgccagag
     2341 ctatcatgtc agcctctgca gatctgacct tctcattctt ggggctgtgt gccccaggtc
     2401 tcctctgcag agaagctctc tgcagcatgg tcacaggatc gctgcaagca gaggcacagg
     2461 gctttctgaa gtcattatca gagtcctaca gagaacagct tccagagtca ctcccagcca
     2521 cccggtgttc gaggcatgac tgtgaacatt gtctccacct gatccttctc tttagaacct
     2581 tctccccagg gctattggac agtttttcct ttcaggaaaa atcatttcca tttaaatttg
     2641 ttttcagtct tacacaaaaa ggagcactca ctacatctga gctaaatgtc ctgggatcag
     2701 tctttgaaac cccacggggt agaagtactt ctgatgacca atctggtgtc atctgagtag
     2761 tgagaataaa gttagttatt ctgaagagcc aagggcttca cagcatccca gagcagggtg
     2821 gccagcatgg gtctcaggcc cagctgctga gaactacagc ttgctcccac ctcttcccta
     2881 gacactttag tagtcccagc tcccagagca gtgatgacag tctgccccag gcagtgctgt
     2941 caccagtctc ggatggacta tgcctataat tgccttttct cccatgtcca taggggccta
     3001 gaagctgtag agccctttcg cagcctggcc tcctgtctga gaggcagggt ttggtctcgg
     3061 gccccgtagg tgcctggaaa tctaatggtt cccaggaatt tcatgaggca aggttcccag
     3121 aggtgttggg tctggcttca gatttttctg gagacccatg ttaagacagc taaaccgaga
     3181 ggtgggacag tcttaggttc ccaccttttc caggtcacaa agctgaagcc ggggttgctc
     3241 tgggagaaga caccacagaa gtgccttcaa gatgagctga gggctccctc cctcgcctgc
     3301 gcagggcctg cattcagtga ggatagcttc cagagcccct gcatgagtgt gcaggattag
     3361 ggaagtgctc gtggggggtg gtcatgccta tctctctgct gcattgctgt tctgttaaac
     3421 caggtcctac catgtgagta atgagatcag aggagcaaga gagctcagag ctgaaggact
     3481 gtgctaactc actgccgtga tggctgactg ctgagcacca tcccaggctt gctcgacctg
     3541 caaacacgct tgttcaggaa ctggagcatg gccaccttgg ccatttggtt tctgagtctt
     3601 caaatggttg tggcccacac ggttagagct cagggttgct ggtgagaact gctgtgagtt
     3661 ccagagcact tacaaggtaa gggccctggc agcttccctg ctttgggtgg tggtttcttt
     3721 tttcttttct ttttttttgt gagagatgta catattttaa tatatataaa gctaagaaaa
     3781 gaac
//
