LOCUS       NM_172540               3261 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TRM2 tRNA methyltransferase 2B (Trmt2b), transcript
            variant 2, mRNA.
ACCESSION   NM_172540
VERSION     NM_172540.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Laptev,I., Shvetsova,E., Levitskii,S., Serebryakova,M.,
            Rubtsova,M., Bogdanov,A., Kamenski,P., Sergiev,P. and Dontsova,O.
  TITLE     Mouse Trmt2B protein is a dual specific mitochondrial
            metyltransferase responsible for m5U formation in both tRNA and
            rRNA
  JOURNAL   RNA Biol 17 (4), 441-450 (2020)
   PUBMED   31736397
  REMARK    GeneRIF: Mouse Trmt2B protein is a dual specific mitochondrial
            metyltransferase responsible for m(5)U formation in both tRNA and
            rRNA.
REFERENCE   3  (bases 1 to 3261)
  AUTHORS   Gendrel,A.V., Tang,Y.A., Suzuki,M., Godwin,J., Nesterova,T.B.,
            Greally,J.M., Heard,E. and Brockdorff,N.
  TITLE     Epigenetic functions of smchd1 repress gene clusters on the
            inactive X chromosome and on autosomes
  JOURNAL   Mol Cell Biol 33 (16), 3150-3165 (2013)
   PUBMED   23754746
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046928.1, AK049523.1 and AK028999.1.
            
            On Nov 21, 2009 this sequence version replaced NM_172540.1.
            
            Transcript Variant: This variant differs in the 5' UTR compared to
            variant 1. Variants 1 and 2 both encode isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028999.1, AK049523.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AK046928.1         1-2
            3-2115              AK049523.1         1-2113
            2116-3261           AK028999.1         2072-3217
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 55.95 cM"
     gene            1..3261
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /note="TRM2 tRNA methyltransferase 2B"
                     /db_xref="GeneID:215201"
                     /db_xref="MGI:MGI:2442530"
     exon            1..139
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            140..370
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            371..425
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            426..560
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            561..660
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            661..731
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            732..878
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            879..973
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1188
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1290
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1410
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1510
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..3261
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3094..3099
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3121
                     /gene="Trmt2b"
                     /gene_synonym="4732479N06Rik"
                     /note="major polyA site"
ORIGIN      
        1 gaggagcctg agcaggcggg ctggagcgct gcgcaccagg aagtgactgg cccctgcaac
       61 cccttcgcgc catccgctgg tattttagtt tgccactgca cgtccgtcca aggccttagt
      121 gactcccaag gagtcccggg catcaagagt cctaaatgca caacccaaga ctattccttt
      181 ccagagcggg tttcttctcc aaaccaggac tgcttccctg ggatactgga agaccaccag
      241 atttgtcaca gtccttattg ggcagagcat ataaagggag tttcaccagc gtgatagcta
      301 agaagcaccc aagcagacaa aaatatcaga agaaacaaag gcattgtaga gatgactcct
      361 ggcaggagag gctggctgat gttgtgactc cactctggag gctgagttat gaagcacaac
      421 tcaaggtgaa atttgaagct cagaagaaac ttttacaaag cctagagtct cacctaaaag
      481 tgctccatgg agtcagtgac acagtggctg cacatcagtc tgaggggctc cgctgtctcc
      541 tccatcccat tataccctct cctaccacca ctggctaccg gaacaagtcc accttttctg
      601 tgtacaggag cccagatggc aatccaaaga ctgtgggcta ctacctagga acttggaaag
      661 atggaaatgt cgtctgtcta ccatgtaatc atctgaaaaa catcccagag aaacacagtc
      721 aagtggctca gtactatgaa gtattccttc gacagtcttc agtggaaccc tgccttctct
      781 ttcatgaagg tggacactgg cgtgaactcg tagttcgtac caataggcaa ggacacacaa
      841 tggctattgt cacttttcat ccccagggat taagtgagga ggaggtctgt gttcagaagg
      901 tgaccttgaa ggattttttc actaaaggcc ctggagcgat ctgtgaattg acctcgctct
      961 atttccagga aagcaccatg acccgttgca gccatcagca gtccccctac cagctcctgt
     1021 ttggggagcc ccacatcttt gaggatcttc tgggtttgaa gattcgaatt tctccagatg
     1081 ccttcttcca gattaatact gctggggcag agatgctgta tcggatcatt ggggagctga
     1141 gtggagtgaa ctctgagagc ctcctcctag acatttgctg tggcacgggt gtgattggcc
     1201 tctctgtggc tcagcgtgcc tcccaggttc atggcattga gctagtggag caggcagtag
     1261 aggatgccag gtggactgca gccttcaatg gtgtcaccaa ctgtgagttt cacgctggca
     1321 gagcagagac gattttgcca cagctgttaa agtcacagaa agatgagaag ttgactgttg
     1381 ctgtggtgaa ccctgcccga gctggattgc attacagagt ggtgcgagcc attcggaact
     1441 gcaggaccat ccacactcta gtgtttgttt cctgcaagcc ccatggtgaa agcacaagga
     1501 acttcattga actatgctgt cctccaaatt ctgcaaagca gctcctaggt gatccattcg
     1561 tcctgagaga agctgtacct gtggatttgt ttccccacac cccacactgt gagctggttc
     1621 tcctcttcac tcggtaagac ctggacgctg caggctgata aggctgaagt ttccgagaca
     1681 cccaggttgc cataggagac agtggaagca tcaccagaaa gaccaatttt gaatcctggc
     1741 caaaagaatt tgactggcct ccttctctga ggcagattgg actgtagctt gtcctagtcc
     1801 tttggggtta tccatattcc cttagctttg attgctccta actccttgaa cctgaatttg
     1861 tgttgctcca tccttttgct gtctatacat cattcaatct acagtcttgt ccccgccatg
     1921 ggattctgct tttgttgtgg ggacaactgc ttttttgagc agccggcagt aatttcatat
     1981 tgactgggac ctgtatattt actctgagat tttaccattt gcattttttg ttgttttgtt
     2041 ttgttttgtt ttgaaacaag gtctcacata gtgcacacta gccttgctct cacaatataa
     2101 ctgaggttga cttcgaactt gtaagccttc tgcctacact ttgtgagtgt tgggcttaca
     2161 ggcatgtgcc accacacatg gctttatgca gtgatgggga tcaaagccag ggaaagatgc
     2221 gtgtgaggga aacccgttta ctaactgagc tacagcccta gccccttgca aatattttta
     2281 ggaagtagaa ccacattgag ctttttacaa agcagaataa gaggctaggg agatggctaa
     2341 ctgggtaaag tgcttatcgc acatacacac aagaagctga atttggatct agcacataat
     2401 aacaaggcat agtggtacct gcctgttctt gtgctgagac gtgtatgcag tgggatcctg
     2461 agggctttcc tggacagtca atttagctaa tagtggttta atgagagacc ctatctcaaa
     2521 aaaagttgaa aaatgataag acacctgata gcgacatctg tcctccacaa gtataatcac
     2581 acacacatac tacacacatg caatgcagac aaagttatag aatgattaca gggttgttgg
     2641 gctttctaga atttgacact tctagaatgt cctttcatga tggggttatc tctggggcta
     2701 gagagatggc tcagtgttca caagcatata atgctcttgc agaggccctg agtttgattg
     2761 ttagtatcca tgtcaagttg ctcattactg cctatgactc tagttccagg gtatctgata
     2821 cccttggctg gtctccatgg acatggtact tatattcaca cacatgcaaa tacacatagt
     2881 ttaaaaataa cttttaaaaa gcttaaagag aaaatgtggt tatctctagg gacaataatg
     2941 ccattcatct cttacctgta gaaggagaat cagtcattag agatccccaa aagggttaaa
     3001 aactcggaaa ggaaggactg aatacttaga ttgtcctgtg tattctggag gaaggaagca
     3061 ccagtggtcc tatagtaatc cagtatgtgt tataataaag tgaatgttga aattaactgt
     3121 attcactatc tctagtccat gtttttttca attctggagt tgtccttgag tacttgtcta
     3181 acattgtctt ttgaatattc taatggtctg aatatatgga agttatgtgt gttgccagaa
     3241 ttacaaaata ataatcaggt c
//
