LOCUS       NM_030016               3767 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus tRNA methyltransferase 13 (Trmt13), transcript variant
            1, mRNA.
ACCESSION   NM_030016
VERSION     NM_030016.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Swinehart,W.E. and Jackman,J.E.
  TITLE     Diversity in mechanism and function of tRNA methyltransferases
  JOURNAL   RNA Biol 12 (4), 398-411 (2015)
   PUBMED   25626150
  REMARK    Review article
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Gu,C., Begley,T.J. and Dedon,P.C.
  TITLE     tRNA modifications regulate translation during cellular stress
  JOURNAL   FEBS Lett 588 (23), 4287-4296 (2014)
   PUBMED   25304425
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK039588.1 and AC131038.6.
            
            On Nov 18, 2017 this sequence version replaced NM_030016.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039588.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3159              AK039588.1         2-3160
            3160-3767           AC131038.6         33578-34185         c
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 50.4 cM"
     gene            1..3767
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /note="tRNA methyltransferase 13"
                     /db_xref="GeneID:229780"
                     /db_xref="MGI:MGI:1925219"
     exon            1..829
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1784
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     CDS             1638..3083
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /EC_number="2.1.1.225"
                     /note="tRNA guanosine-2'-O-methyltransferase TRM13
                     homolog; tRNA [Gm4] methyltransferase; coiled-coil
                     domain-containing protein 76; coiled-coil domain
                     containing 76; tRNA methyltransferase 13 homolog"
                     /codon_start=1
                     /product="tRNA:m(4)X modification enzyme TRM13 homolog"
                     /protein_id="NP_084292.1"
                     /db_xref="CCDS:CCDS38612.1"
                     /db_xref="GeneID:229780"
                     /db_xref="MGI:MGI:1925219"
                     /translation="MEAPAATSPSIGFPIDGRCNYFVEKKKRFCRMVAAAGKRFCGEH
                     AGSAEEENTRKRILCPLDPKHTVYEDQLAKHLKKCNSREKPKPDFFIQDINAGLKDET
                     EIPEQLVPFSSLSEEQLENLIKKLRKASEGLNSTHEDHIMSHPALHDALNDPRNGDCA
                     VKHLKQQASILGNIEKLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAENVHFILVERV
                     TTRFKVDGKHRKKDSVFERLQIDIQHLCLNRVPVLREGRLPVVGIGKHLCGVATDVAL
                     RCLVETYAASFEEKDEEPLAKRIKNDKTEKESNTLAKEGSEKDVPETWTPVAGIVIAL
                     CCHHRCDWRHYVGKEYFKALGLGAVEFYYFQRMSSWATCGMRTSLEGSDVTPERKDAQ
                     RDENEEHDDGGDRLTDGNTDSLPGILTVEEKKKIGHLCKLLIDQGRLQYLQQKGFSPA
                     LQYYTDPLVSLENVLLTAVPAHPSSQEKHHQ"
     exon            1785..1831
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1898
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..1961
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2031
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2138
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2306
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2379
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2454
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2884
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3767
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3375..3380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /note="hexamer: AATAAA"
     regulatory      3384..3389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /note="hexamer: TATAAA"
     polyA_site      3402
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /note="major polyA site"
     polyA_site      3406
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
     regulatory      3734..3739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
                     /note="hexamer: AATAAA"
     polyA_site      3767
                     /gene="Trmt13"
                     /gene_synonym="4631408H19Rik; A330067P21; A930028L21Rik;
                     Ccdc76"
ORIGIN      
        1 gagtaacaac agaactctgt gttgcagcct cagcatctct gcagtgaggt taagggtggc
       61 acctaaatac ggggttcatt tctggaagtc acttccacag tgtctgtggt tacctcgcac
      121 atgctcagta agcaacagct actgtctagc agtagtatcc tcactggtcg ttccacttca
      181 tagagctgct ggactacact tgccaacgct gtgagacaca gacactgcag taatattaaa
      241 aggaactgaa aggatctgag ggctgctccc gtgaatattc caactccaca ctaaaatacc
      301 cagtctgttt ccatgctgtc ctgaaccagc tccgtcatca cacttgccac tccctgtttt
      361 ctctgcagtg agatgatcac tgtagggaca gaagttctgt gcagagcatc caagggaaca
      421 gcagccactg agcagcacag tagccacact aaagccgagg gagcccgcaa cagcagccag
      481 cacgggatac ctcttccacg ccagccagct tcgcctttag ctctcggaca gtcgagtctc
      541 ctttatactt cctctcggtg agctctttat tagctgcctc taactcggac aatctgcttt
      601 gtagctggtg gatattccgt tgatggaggg tttccagttc ctttttctgt tgctcgtgct
      661 gctgttggca ctgaacttgg gtctattata gagcagtatg tcaaaggaga aagagcagaa
      721 gtataaagat cagaaaaaac aaaacatcat ttcttcatcc taatttcttc atcgttttcc
      781 aatttccctg cttagaagaa gtctaattac agatttcacg aaagagcaga tggagttcta
      841 aagcttcacc cccagtgttc ttcaaaggag agcatgagtg aagggccggg cgacctgaat
      901 cctagtgcgg acagctccct gaagtcaccg tgaagctttt gtggaaatga cagggagaat
      961 cagagttgcc acccagaaag gacaggctgg gctctgtccc gctgagggcc agaacacgga
     1021 cgtgaaggag tcggcttgcc atttcctcat tccctttcct cgcgtcccct ccgcaacggc
     1081 cggcccggcc acaaacaaag gtatccatta agtccgcagg gtcccttccc gattcccggt
     1141 gtgacagtcc gacacccgcg gaccgaccga tccccacgga acccagccgc catcttcccc
     1201 attggcgcga ccccaaccgc cacccgccgg ctccgggagc tcacctctcc tcgcagtctt
     1261 tgcatttcac tagcaaggga acaagctgct ggaacagaac ttgactcatg tcggaccgct
     1321 gccccgacgg gagggtgaag aagcccagta ggcccggcct tcggtcccgg ctgcaaagaa
     1381 gagccggtcc cgatgagttt gagtttggcg ctcagctccc gcggaggagg agggaaacga
     1441 taaacagagg gaacgaagga gctttggacc tcgtcagcca ccgtcacctt tttccggttc
     1501 gggcaccgac cgagccgggc tgatgcgtca tttccgtgga cccacccccc tcaggcggcc
     1561 acgtttgtta agggaagtgg gaccaaaggg gactgacaac tttgggctga gccaggcttt
     1621 caaaaggacc cagcgttatg gaggctccgg cggcgacttc gccctcgatc ggatttccca
     1681 tcgacggcag atgcaactac ttcgtagaaa agaagaagcg cttctgcagg atggtggcgg
     1741 ccgcggggaa aaggttctgt ggcgaacacg ctggctccgc cgaggaagaa aatactcgga
     1801 aaagaatctt gtgtccttta gatccaaaac acacagtgta tgaagaccaa ctagcaaaac
     1861 atttgaaaaa atgtaattca agagagaagc ctaagcctga tttctttatt caagatataa
     1921 atgcaggatt aaaggatgaa acagagattc ctgagcaatt agttccattt tcttctctat
     1981 ctgaagagca gttggagaac ttaattaaga aattgagaaa agcaagtgaa ggcttgaatt
     2041 ctacacatga agatcacatc atgtcccatc cagcattgca tgatgccctt aatgacccaa
     2101 ggaatggcga ttgtgcagtc aaacacctga aacagcaggc ttctattcta ggcaacattg
     2161 aaaagttaaa gttacttggc ccaagaagat gctttgttga gtttggagca ggaaagggga
     2221 aattatctca ctgggttgat attgccttaa aagatgctga aaatgttcac ttcatcctgg
     2281 tagaaagagt gaccacaaga ttcaaggtag atggtaaaca cagaaaaaag gactcagtgt
     2341 ttgaaaggct tcaaattgat atccagcact tgtgtttgaa cagggttcct gtgctaagag
     2401 aaggaagact gcccgtggta ggaattggga agcacctgtg tggggtggcg acagatgttg
     2461 cattacgatg tttggttgaa acctatgctg ccagttttga ggaaaaggat gaagaacctt
     2521 tagccaaacg cataaagaat gataagacag aaaaagaaag taacactttg gccaaggaag
     2581 gaagtgaaaa agatgtccca gagacatgga ccccggtggc tggcattgtg attgcgctct
     2641 gttgtcacca caggtgtgac tggagacatt atgtgggcaa agagtatttc aaggctcttg
     2701 gacttggggc ggtagagttc tactatttcc agagaatgag tagttgggca acgtgtggaa
     2761 tgaggacatc gttggaaggc tcggatgtta ctccagagag aaaagatgct caaagggatg
     2821 agaatgaaga gcacgatgat ggaggagaca gactcacaga tggcaacact gacagtttgc
     2881 ctgggattct tactgttgaa gaaaagaaga aaataggaca tctttgtaaa ctgctgattg
     2941 accaaggcag actccagtat ttacagcaga aaggcttcag tcctgcctta cagtactata
     3001 cggacccatt ggtgtctctg gaaaacgtat tattaactgc cgtaccagct catccgtcat
     3061 cgcaggaaaa acatcatcaa tgaaaggaaa catgaacgtg acgtttgtct tccacaaaca
     3121 aaaaaattta aattattttt aaattatatt tcgatattca caaaaatatt caccaagtag
     3181 tcaaagtatt ccttttcagc agggctcttt caattacttt ctccatcaga atgtatagag
     3241 cttggcattt gagtcagaca gttgacttgg tcttctgaaa gatataacac cagtatataa
     3301 ttatagtgtg aactctgtgt attaatctgg taaaggtttt atttgatttt tttcattttt
     3361 tgagctccag ttataataaa cagtataaat ttataacaag gatcaatctg agtgtgaggc
     3421 tttttttttt ttcttttttt ctttttttct tttttctttt ttccccgaga cagggtttct
     3481 ctgtgtagcc ctcaatgtcc tggaactcac tttgtagacc aggttggcct caaactcaga
     3541 aatccgcctg cctctacctc ccaagtgctg ggactaaagg cgtgcgccac cacgcctgag
     3601 tgaggctttt tttttttttc aatataggtc tagaaagaat aattgtaatt atcttgaaca
     3661 cagacaaatt tagaaattta agaattggca gccgggcgtg tgtggtggca cacgccttta
     3721 attctaagtg ttgaataaag tccatgttct cagtattttt cacccca
//
