LOCUS       NM_001404386            4030 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus tRNA methyltransferase 10A (Trmt10a), transcript
            variant 22, mRNA.
ACCESSION   NM_001404386
VERSION     NM_001404386.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Tresky,R., Miyamoto,Y., Nagayoshi,Y., Yabuki,Y., Araki,K.,
            Takahashi,Y., Komohara,Y., Ge,H., Nishiguchi,K., Fukuda,T.,
            Kaneko,H., Maeda,N., Matsuura,J., Iwasaki,S., Sakakida,K.,
            Shioda,N., Wei,F.Y., Tomizawa,K. and Chujo,T.
  TITLE     TRMT10A dysfunction perturbs codon translation of initiator
            methionine and glutamine and impairs brain functions in mice
  JOURNAL   Nucleic Acids Res 52 (15), 9230-9246 (2024)
   PUBMED   38950903
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4030)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4030)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4030)
  AUTHORS   White,J.K., Gerdin,A.K., Karp,N.A., Ryder,E., Buljan,M.,
            Bussell,J.N., Salisbury,J., Clare,S., Ingham,N.J., Podrini,C.,
            Houghton,R., Estabel,J., Bottomley,J.R., Melvin,D.G., Sunter,D.,
            Adams,N.C., Tannahill,D., Logan,D.W., Macarthur,D.G., Flint,J.,
            Mahajan,V.B., Tsang,S.H., Smyth,I., Watt,F.M., Skarnes,W.C.,
            Dougan,G., Adams,D.J., Ramirez-Solis,R., Bradley,A. and Steel,K.P.
  CONSRTM   Sanger Institute Mouse Genetics Project
  TITLE     Genome-wide generation and systematic phenotyping of knockout mice
            reveals new roles for many genes
  JOURNAL   Cell 154 (2), 452-464 (2013)
   PUBMED   23870131
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131122.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.8758506.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849382,
                                           SAMN00849383 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AC131122.4         48558-48701         c
            145-340             AC131122.4         44584-44779         c
            341-510             AC131122.4         43631-43800         c
            511-582             AC131122.4         42629-42700         c
            583-657             AC131122.4         41677-41751         c
            658-807             AC131122.4         39813-39962         c
            808-913             AC131122.4         37356-37461         c
            914-4030            AC131122.4         32333-35449         c
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 64.09 cM"
     gene            1..4030
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /note="tRNA methyltransferase 10A"
                     /db_xref="GeneID:108943"
                     /db_xref="MGI:MGI:1920421"
     exon            1..144
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            145..340
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            341..510
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            511..582
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            583..657
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             622..1152
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /EC_number="2.1.1.221"
                     /note="isoform 4 is encoded by transcript variant 22; RNA
                     (guanine-9-) methyltransferase domain containing 2; tRNA
                     methyltransferase 10 homolog A; tRNA
                     (guanine(9)-N(1))-methyltransferase TRMT10A"
                     /codon_start=1
                     /product="tRNA methyltransferase 10 homolog A isoform 4"
                     /protein_id="NP_001391315.1"
                     /db_xref="GeneID:108943"
                     /db_xref="MGI:MGI:1920421"
                     /translation="MDENDQGWVNWKDIHIKSEHYSELIKKEDLVYLTSDSPNVLKDL
                     DESKAYVIGGLVDHNHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVLAVNHVFEII
                     LEFLETRDWQEAFFTILPQRKGAVPAHKACESSPQDHQSLPEGWDSASEGESCRDNPV
                     SPQKDEQGQQSSPVLQ"
     exon            658..807
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..913
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     exon            914..4030
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2463..2468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /note="hexamer: AATAAA"
     polyA_site      2486
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /note="major polyA site"
     regulatory      4004..4009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
                     /note="hexamer: AATAAA"
     polyA_site      4030
                     /gene="Trmt10a"
                     /gene_synonym="3110023L08Rik; Rg9mtd2; Rnmtd2"
ORIGIN      
        1 gtaagtgccg taaccgggcg cgcaaccacc gcctctgcct ctgggagttg aagtttctga
       61 aggaggacga ccgcgtggag ccgagaacac ttcagaaagg ctttctaacc gcaccccggc
      121 gttccccacc ggccccgcgg cacgagacaa tgaatatgtc atctgaaatg ctgccagcat
      181 ccatcgagtc tactaatgtg gaggaaaagc tgggtacgag tgacggtgaa gaggagagac
      241 aggagcctag agtggatgca ggggctgagc caatatctaa acggcaactg aagaagctga
      301 tgaaacaaaa acagtgggaa gagcaacgtg aacagcgcaa ggagaaacga aaagaaaaac
      361 ggaagagaaa gaaattagaa cgacgccagc tggagtccaa ctcagatggc aacgacagga
      421 agcgcgttcg gagagatgtt gctcgcagca gcctccgcct ggtcatcgac tgtagctttg
      481 atgacttgat ggtgttaaag gtatcatgag gatattaaga aacttcataa acagattcaa
      541 cgatgttatg cagaaaaccg ccgagcctct catccagtgc agttttactt gacaagccat
      601 ggaggtcaac tgaagaagaa catggatgag aacgaccaag gatgggtcaa ctggaaggat
      661 attcacatca agtcagagca ctatagtgaa ctcataaaaa aagaagacct ggtttatctg
      721 acgtcagact cacctaatgt actgaaggac ttagatgagt caaaggccta cgtgattgga
      781 gggctagtgg accacaacca tcacaaggga ctcacattta aacaagcaac cagttacgga
      841 atcgaacatg cacagctccc tcttgcagat tttgtgaaga tgaacagtcg aaaagtcctg
      901 gccgttaacc atgtgtttga aattatcctg gagttcttgg aaacaagaga ctggcaagag
      961 gcattcttta caatcttacc ccaacggaaa ggggccgttc ctgctcacaa ggcctgcgaa
     1021 agctctcctc aggaccacca gtccctgcca gaaggatggg acagtgccag cgagggagag
     1081 agttgcaggg ataatcctgt ctcaccacag aaagacgagc agggccagca gagcagcccg
     1141 gttctgcagt gagccccgtc ccacagtaac cttcccctgt cctcctctcg cttaagagaa
     1201 accaggcagt gcggtgcttt acagaatgct cagactgcaa gaaaactttc tctagtctat
     1261 gttgtggaat tttaaatctg ttttaagagt taaataataa tgaaaaaaac cctttaaaca
     1321 ctaagaaagg atcgtttggt tttgtgtaag aagacttaaa gatatgattt caaaattgct
     1381 ttaagcctca aatcagtcat ggaggacgtg gaatacgagt ctgcctcggt tcgtcactca
     1441 ctggagcata tccttcagga cgtgtcttct gacccttgag cttggttctt taagttccta
     1501 cctgtatctt tttagtccga tgtctatttg gtggagcaga tattcataac tgacctgtgt
     1561 gtcttctaga atgttgaact ttggaagcta tgcttttttc tgtagctttg gtgttttgtc
     1621 taagaaatac attaattcta ggagaactac cagttgtttg ctgggctcta caattcattc
     1681 cttttggaca atggagtcta ttcctgtgcc tcccttgtga taccttgtta tgcttctgaa
     1741 atagtcacaa gaataatctt aaatcctgta atgacaataa cttattcaat tacacagcta
     1801 agttttgctg gctagattat tatataagct ttttttgaag gatcaatttg tctttacctc
     1861 agtttaaaac aaaacaaaaa agttatcctt ctcatatatt ttccatatat atatatacca
     1921 tatatttcca taatgttatc ttttaatgtg aatattatac atctgcctcc aactaatata
     1981 aatgtttctg taaatagcat atgcatgtag gaaaatgagc atgtaataat gtttacatct
     2041 gtccatatca catttagcct tcatgcctta atccacaatg acaggactga tgcattcttt
     2101 gcggaatggg tgtgttgaat tcactagttt gaattcgtta aatggaattg aacagtggtt
     2161 tggaaaccga tggagacaga gcagagggca ctgcactgtt gggcttctgc agagagtttg
     2221 cagagtagta ttcaggtcac aagcccttcg cacgagtgca gtggagctcc aggggctctc
     2281 attggtcttt tctctttatg atacaatgtg tgcgctgtgt tagcatctca cttacacaga
     2341 cgctcatgac atttggtgtt ggtggtcagt gcctattgct ttattcttag ttacctattg
     2401 tagctagctc tttcaaagtc tataaatgaa gtatgtaaac agtcacagta tagttctaaa
     2461 tgaataaagt tcatgttgtt actgtacttt tctaaaattc aggttttgtg aggtacaccc
     2521 catgggcccg tgtgtttgga cacttggtct ccagctgatg gtgccgtttt cgaggttgtg
     2581 gggcctttca aaggggaagc tttgctggag ggagcatgtc acttagggtg ggcttttctt
     2641 tcttccttct ttctttcttt ctttctttct ttctttcttt ctctttcttt ctttctcttt
     2701 ctttcttttc tttccttcct tccttccttc cttccttcct tctttccttc tttctttctt
     2761 tctttctttc tttctttttt tttttttttt tttggttttc tgagacaggg tttctctgtg
     2821 tatctctggc tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagagatcc
     2881 gcctgcctct gcctcccaag tgctgggatc aaaggtgtga gccaccactg cctggtgggc
     2941 ttttcaactc tatagccagt cctgtttttt gtgtcttctt tcctttatac aggcaaatgt
     3001 gatcaccacg tcctgacatc caaaccaacc tgacaatccc actgccaggc ctcccctgca
     3061 gtgagggtct gtatacctcc tcagagctct agaccaaaat aaccctccct tccttattgg
     3121 ccttttgtca gggtgttttg tcatgaagta acataaaagt atctaacaaa aacatatcat
     3181 tttatatggg agaggaccct ataaaagtga aggaaatcat ttcaagtgcc acatggtttc
     3241 agctcactca ttgcaaaagc tctgttgatt tcctgggcat gctataagaa aacaccacag
     3301 acctagtggg ctaaaataac cacaacttat tttctacagt gtcagaggct gcaagttcag
     3361 aatcaagcct ggtagggtca tgctccctct ggctcacaag acgttctttt taaccttaat
     3421 gtattttttt ctgtcaactt tttgttttcc agtacagcag cagatgaaaa ggagaaaata
     3481 ggtaacaatg atttgaatta atggcagcag agaccatgag ctcagtgagt gagctagctc
     3541 atggaggtct ttctccctct ttctagctca tttcgagggc atgcgccgtg agcttaatat
     3601 gtgtgaagaa gacagtgatg gtcgcctcaa gagtcgggat tgttgctggg gagatcaggg
     3661 gattggatga ggcgtaagcc ttgtactctt atgcttgtat tgtgacactg ggtatgttag
     3721 aactcagtgt ccctatttta aatgtgactg ttacatattg gtcctgtcag cagcgtgtga
     3781 ggtaggcaat ggggacttgg tgactttctt cctctccttg tttctcttct ctcttgacct
     3841 tgccagctct aaaatatcag taaacctgct tcagactttt tgttcttttg gggtacaagt
     3901 cttgacaaga ttttaaaaat ctgctttctg ccagaacagg ggcaaagaac taaatgcata
     3961 gcatttcata aaggctttat tacaacagta aaaacaatgc taaaataaaa cacaaccatg
     4021 aaagaccaaa
//
