LOCUS       NM_001164559            2159 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus tRNA methyltransferase 1 (Trmt1), transcript variant
            2, mRNA.
ACCESSION   NM_001164559
VERSION     NM_001164559.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2159)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CN528783.1, AK159467.1 and BB055133.2.
            
            Transcript Variant: This variant (2) uses a different splice site
            in the 5' UTR, compared to variant 1. This variant encodes isoform
            1. Variants 1 and 2 encode the same protein.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is also supported by available conservation
            data. The use of an alternative upstream start codon would result
            in a protein that is 38 aa longer.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159467.1, SRR1660819.51595.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                CN528783.1         382-417
            37-2138             AK159467.1         2-2103
            2139-2159           BB055133.2         694-714
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.02 cM"
     gene            1..2159
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /note="tRNA methyltransferase 1"
                     /db_xref="GeneID:212528"
                     /db_xref="MGI:MGI:1289155"
     exon            1..103
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            104..380
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2115
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /EC_number="2.1.1.216"
                     /note="isoform 1 is encoded by transcript variant 2;
                     n(2),N(2)-dimethylguanosine tRNA methyltransferase;
                     tRNA(m(2,2)G26)dimethyltransferase;
                     tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA
                     2,2-dimethylguanosine-26 methyltransferase; tRNA
                     (guanine(26)-N(2))-dimethyltransferase; TRM1 tRNA
                     methyltransferase 1 homolog"
                     /codon_start=1
                     /product="tRNA (guanine(26)-N(2))-dimethyltransferase
                     isoform 1"
                     /protein_id="NP_001158031.1"
                     /db_xref="CCDS:CCDS40410.2"
                     /db_xref="GeneID:212528"
                     /db_xref="MGI:MGI:1289155"
                     /translation="MSLARTILWLSRPLRPAHSLCRAQFMERKAQKPPSPPAMENGTR
                     SWEERPPADPVATVTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEFARIHLGAKG
                     IQIKVPGEKDSEKIAVDLSDQEEETAGKNENLAPGDWPRTAAVGEICEEGLRVLEGLA
                     ASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNGVAHLVQPNQADARMLMYQ
                     HQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGETCYSKY
                     GAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQAKV
                     KSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQL
                     GGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQL
                     SSTIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVK
                     RERLSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRARP
                     GGKAASEDLAGRRRLLQNKRKEPAEDPAQRAARLKTFPCKRFKEGTCQLGDQCCYSHS
                     PAAPVASGDIPIEECPETTTKISPGPKAAAGGIPGPGVD"
     exon            381..436
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            437..573
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            574..761
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            762..877
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            878..990
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1139
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1226
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1296
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1431
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1517
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1626
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1703
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1823
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1953
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2159
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2119..2124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /note="hexamer: AATAAA"
     polyA_site      2138
                     /gene="Trmt1"
                     /gene_synonym="6720406L13Rik; 6720477L24; D8Ertd812e"
                     /note="major polyA site"
ORIGIN      
        1 ctaattggtc agttattgtc atgtgattgg cccgccctct ttgccaacat ggcggctccc
       61 aggccctaat gcgagaagca tgcaccgtga ctcgttggat cctgcgggtt gaggcgcccg
      121 cggatgtccc tcgcgaggac gatcctgtgg ctaagccgtc ctctccgtcc ggcccacagc
      181 ctctgtagag ctcagttcat ggaacggaag gcccagaagc ctccctctcc accagcgatg
      241 gagaacggca cccggtcctg ggaagagcgc ccacccgcgg acccggttgc gactgtcact
      301 gagggcgccg ccaaaatcgt ctttcccagt gctaacgagg ttttctataa cccggtgcag
      361 gaattcaacc gggacctgac gtgtgctgtg atcacagagt ttgctcgaat tcatcttggg
      421 gccaagggaa tccagatcaa ggttccagga gagaaggact cagagaaaat agctgtggac
      481 ttgtctgatc aagaagagga aacagctgga aagaatgaaa atctggcccc aggggactgg
      541 ccacggactg ctgcagtggg tgagatctgt gaggaaggtc tacgagtgct ggaaggcctg
      601 gcagcttccg gtctgcgctc catccgtttt gccctggagg ttcctggcct ccagtctgtg
      661 gttgccaatg atgcctctgc ccgggctgta gagcttatgc acagaaatgt ggagctcaat
      721 ggtgtggcac atcttgttca gcccaaccag gcagatgccc ggatgctgat gtaccaacac
      781 caaaaggcac ctgagcgatt tgatgtcatt gacctggacc cctatggcag ccccgccccc
      841 ttcctggatg cagcagtgca ggctgtgagt gatggaggac ttctgtgtgt cacctgcaca
      901 gacatggcag tcctggcggg gaacagcgga gagacatgct acagcaagta tggggccatg
      961 gccctcaaga gccgagcctg ccatgaaatg gccctgcgga ttgtgctgca cagcctggac
     1021 ctgcacgcca actgctacca gcgctatatc gtgccgctgc tcagcatcag tgctgacttc
     1081 tatatccggg tctttgtacg tgttttcaca ggccaggcca aggttaagtc ctcagccagc
     1141 aaacaggccc tggtgttcca gtgtgtgggt tgtggagcct tctacctgca acgccttggc
     1201 aaagcatcag gagaccctgg tggcaggatc aagttctctg cagcctgtgg tcctccagta
     1261 actcccgagt gtgagcactg tggacagaga caccagcttg ggggccccat gtgggcagag
     1321 cccatccatg acctggactt tgtgggccga gttcttgacg cagtgaccac gaatcctggc
     1381 cgcttccaca cttccatgag gatccaaggc gtcctcagtg ttgttactga ggaacttcct
     1441 gatgtccctc tctactacac actggaccaa ctgagcagca ctatccactg caacacaccc
     1501 cgccttctgc agcttcggtc agctctcctt catgctggtt tccgagtctc tctctcccat
     1561 gcttgtaaga atgcagtgaa gacagatgct ccccctgagg ccctctggga catcatgcgt
     1621 tgctgggaaa aggaatgtcc agtgaaacgg gagcggctgt ctgaaagcag tccagcattc
     1681 cgaattcttg ctgtggagcc caggctgaag gccaacttca acatccggga agacgccaac
     1741 cccagctccc gccagcgagg acttaagcgc ttccaggcca atccagaagc caactggggt
     1801 ccccggcccc gtgcccggcc agggggcaag gcagcaagtg aagatctagc agggaggcgg
     1861 aggctgctac agaataagag gaaggagcca gctgaggacc cggcccagcg tgcagctcgc
     1921 cttaagacat ttccttgcaa acgattcaaa gagggtacct gtcaactagg ggaccagtgc
     1981 tgttattcac acagtcctgc agctcctgtg gcctctggtg atatccccat cgaagaatgt
     2041 ccagagacta ccaccaagat ctcccctgga ccaaaggctg ctgctggggg tattcctggg
     2101 ccaggtgtag actgagacaa taaagagatg taacctgctg gttgtcattt tgaattggg
//
