LOCUS       NM_001347095            1467 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus tRNA methyltransferase O (Trmo), transcript variant 2,
            mRNA.
ACCESSION   NM_001347095 XM_006538325
VERSION     NM_001347095.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1467)
  AUTHORS   Eckey,M., Kraft,F., Kob,R., Escher,N., Asim,M., Fischer,H.,
            Fritsche,M.K., Melle,C. and Baniahmad,A.
  TITLE     The corepressor activity of Alien is controlled by CREB-binding
            protein/p300
  JOURNAL   FEBS J 280 (8), 1861-1868 (2013)
   PUBMED   23441852
REFERENCE   4  (bases 1 to 1467)
  AUTHORS   Moreno,L.M., Mansilla,M.A., Bullard,S.A., Cooper,M.E., Busch,T.D.,
            Machida,J., Johnson,M.K., Brauer,D., Krahn,K., Daack-Hirsch,S.,
            L'heureux,J., Valencia-Ramirez,C., Rivera,D., Lopez,A.M.,
            Moreno,M.A., Hing,A., Lammer,E.J., Jones,M., Christensen,K.,
            Lie,R.T., Jugessur,A., Wilcox,A.J., Chines,P., Pugh,E., Doheny,K.,
            Arcos-Burgos,M., Marazita,M.L., Murray,J.C. and Lidral,A.C.
  TITLE     FOXE1 association with both isolated cleft lip with or without
            cleft palate, and isolated cleft palate
  JOURNAL   Hum Mol Genet 18 (24), 4879-4896 (2009)
   PUBMED   19779022
REFERENCE   5  (bases 1 to 1467)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL806523.14.
            
            On Nov 15, 2016 this sequence version replaced XM_006538325.3.
            
            Transcript Variant: This variant (2) differs in the 3' UTR and
            coding sequence compared to variant 1. The resulting isoform (2)
            has a shorter and distinct C-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092543.1, AK052584.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AL806523.14        216274-216393       c
            121-295             AL806523.14        214524-214698       c
            296-453             AL806523.14        213050-213207       c
            454-1080            AL806523.14        209015-209641       c
            1081-1467           AL806523.14        206880-207266       c
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 24.71 cM"
     gene            1..1467
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /note="tRNA methyltransferase O"
                     /db_xref="GeneID:74753"
                     /db_xref="MGI:MGI:1922003"
     exon            1..120
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..1340
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     nef-associated protein 1; likely ortholog of H. sapiens
                     chromosome 9 open reading frame 156 (C9orf156);
                     thioesterase NAP1; tRNA
                     (adenine(37)-N6)-methyltransferase"
                     /codon_start=1
                     /product="tRNA (adenine(37)-N6)-methyltransferase isoform
                     2"
                     /protein_id="NP_001334024.1"
                     /db_xref="CCDS:CCDS84727.1"
                     /db_xref="GeneID:74753"
                     /db_xref="MGI:MGI:1922003"
                     /translation="MRGLEKQGSCATAAPCGCVQPALEAGNLLTEPIGYLESCFPAKI
                     GTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHKNGHLNYKAKVQ
                     PPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVYLSGVDMIDGTPVLDIKPYI
                     ADYDSPQNLSVHNDHHKLRAEAQVDGTANSCDQLLLSGRGKVQPRQSTKERPKCLEDR
                     TSGENSQKSRDMSEIQHTLPEDRERALDLALEPSRGESMDMPENQLGPPELKSFLEEG
                     TDRPRKVEGALVLPGSSAETQWDASYRARTADRVPYSVVPSWVTEAPVAPLQVRFTPH
                     AEMDLRKLNSGDASQPSFKYFHSAEEAKRAIEAVLSADPRSVYRRKLCEDRLFFFTVD
                     TAHVTCWFGDGFAEVVRIKLASESVQVADPEESLAALGS"
     exon            121..295
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            296..453
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            454..1080
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1467
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1441..1446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /note="hexamer: TATAAA"
     polyA_site      1465
                     /gene="Trmo"
                     /gene_synonym="5830415F09Rik; Nap1"
                     /note="major polyA site"
ORIGIN      
        1 ggaaatcagc gttgggcgcg cggacgactc agcggcctgt aaccatgcgc ggcttggaga
       61 agcagggctc gtgtgctacg gcggccccat gtgggtgcgt tcagccggcc ctggaggcag
      121 ggaatctttt aactgagcca atagggtact tggaatcatg tttcccagcc aagattggca
      181 ctccaaggca gccatccatc tgtagccact ccagagcctg tttgaagatc agaaagaaca
      241 tctttaataa ccctgagcat tccttgatgg gcctagaaga gttttctcat gtttggattt
      301 tgtttgtttt tcacaaaaat ggccatttga actacaaggc aaaagtgcag ccgcccaggc
      361 tgaatggggc aaagactgga gtgttctcca cacggagccc tcatcgccct aatgccatag
      421 ggctgacgct ggccaagctg gagaaggtgg aaggtggagc tgtctacctg tctggagttg
      481 acatgataga cggcacacct gtgctagaca taaagcccta cattgctgac tacgactcac
      541 cacagaacct cagtgtacac aatgaccacc ataagctgag ggctgaggcc caggttgatg
      601 gcacggccaa cagttgtgac cagctgctgc tctcagggcg tgggaaagta cagcctcgcc
      661 agagcaccaa ggagagaccg aaatgccttg aagacaggac ctcaggagaa aactctcaga
      721 agtccagaga catgtcagaa atccagcaca ctttgccaga agacagagag agagcgttgg
      781 atctggcact ggaaccaagc cgaggtgaga gcatggatat gcccgaaaat cagcttggcc
      841 caccggaact gaagagcttt ctggaagaag gcacagatag accaaggaaa gtagaaggtg
      901 ccttggtcct gccggggagc agtgcagaaa cacagtggga tgcttcctac cgtgccagga
      961 cagctgacag agtgccctac agtgttgtcc ccagctgggt gacagaggct cctgtagccc
     1021 ctttacaagt ccggtttact cctcacgcag agatggatct gaggaagctc aattcaggag
     1081 atgctagtca gccgtcattt aagtacttcc actccgcaga ggaagccaag cgtgccattg
     1141 aggctgtgct gtcagcagat cctcggtctg tgtaccgccg gaagctctgc gaggaccgcc
     1201 ttttcttctt cactgtggac acagcacatg tcacctgctg gtttggtgat ggcttcgcag
     1261 aggttgtgag gattaaactg gcttctgagt ctgttcaggt ggctgaccct gaggaatcct
     1321 tggcggccct ggggtcttga gcccactgtg ttttcagaag ctccattggc atctgatgtg
     1381 cctgctcttg tcatcttact gctttggttg gtgctggttt ttgtttttct ttttttcttt
     1441 tataaagaca ttatttattt tattata
//
